MyabA.00951.b

Putative amidase AmiC

CENTER ID: MyabA.00951.b
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00951.b.A1.GE30379 Full length( MyabA.00951.b ) 1 471
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.765
RefSeq: YP_001701474.1
UniProt: B1MHZ8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTRIHAFTED ALGDLDATGV AERIASGEVT AAECAEAAIV RLERVNPTLN AVEFKDFPRA RERAEQADAA SSTSGTFRGV PTATKCNIHV AGMPLTEGSA AITPAPQTDD GPFARQFLST GVIPIASTTM PEYGWSATTE RAGGSATRNP WHTDFSAGGS SGGSAALVAA GVVPIAHGND GGGSIRIPAA ACGLVGLKPS RGRLLGDPSS SSAPVKIVAE GVLVRSVRDC ARFFESAELH YRNPKLTPIG HVDRPVQRRL RIGLCMDSPF TPATDNPTRQ AVNSAAELLE SLGHTVEPYT PAVLPSFKID FEDYWAFLAF AVAKGGPRLF EGFDPAKLDR LTLGLSRRFV RRSYRTPLFL ARLAAAARVY ERGFTTGIDA VLTPVLTHTT PRIGYLTPDQ DPDVLLDKLS SYVGFTPVHN ASGAPAISLP LGQDPDGLPI GVMFSGRRGS ERTLLELAME IEEAQPFSIL G
NT Sequence
ATGACCCGCA TCCACGCGTT TACCGAGGAC GCCCTGGGCG ACCTGGATGC CACCGGAGTC GCCGAGCGAA TCGCCTCCGG CGAGGTCACT GCCGCCGAAT GCGCCGAGGC CGCCATCGTC CGTCTCGAGC GGGTCAACCC GACGCTCAAC GCAGTGGAAT TCAAGGATTT TCCGCGCGCC CGCGAGCGCG CCGAACAGGC AGACGCCGCG TCCAGCACTT CCGGCACTTT TCGGGGTGTG CCCACCGCGA CGAAATGCAA TATCCACGTG GCGGGTATGC CACTGACGGA AGGCTCGGCG GCGATCACGC CGGCACCGCA GACTGACGAT GGACCGTTCG CACGACAATT CCTCAGCACC GGCGTGATCC CCATCGCCTC GACGACCATG CCCGAATACG GCTGGTCTGC CACCACGGAG CGCGCAGGTG GCTCGGCTAC CCGCAATCCG TGGCATACGG ACTTCTCTGC CGGCGGATCC TCCGGCGGGT CGGCGGCACT GGTAGCCGCC GGTGTGGTGC CCATCGCACA CGGCAATGAC GGCGGCGGCT CGATCCGGAT CCCCGCGGCG GCCTGCGGAC TCGTCGGCCT CAAGCCGAGC CGCGGAAGGC TTTTGGGCGA CCCGTCGAGC AGTTCGGCCC CAGTGAAAAT CGTCGCCGAG GGGGTGCTGG TGCGCAGTGT GCGGGACTGT GCCCGCTTCT TCGAGTCGGC CGAACTGCAC TATCGCAACC CAAAGTTGAC ACCGATCGGG CACGTGGATC GCCCGGTCCA GCGCCGGCTG CGCATCGGCC TATGCATGGA TTCCCCCTTC ACTCCCGCCA CCGACAACCC AACCCGGCAG GCAGTGAACT CCGCCGCGGA ATTGCTTGAA TCCCTTGGCC ACACCGTCGA ACCGTACACA CCCGCCGTGC TTCCGTCGTT CAAGATCGAC TTCGAGGACT ACTGGGCCTT CTTGGCCTTC GCCGTGGCTA AGGGTGGTCC CCGGCTGTTC GAGGGGTTCG ACCCCGCTAA GTTGGACCGG CTGACCCTCG GGCTCAGCCG CAGATTCGTC AGGCGCTCGT ACCGCACGCC GCTCTTCTTG GCACGCCTGG CCGCCGCCGC CCGGGTATAC GAACGCGGCT TCACCACCGG TATCGATGCG GTGCTCACGC CCGTGCTCAC CCACACCACG CCACGCATCG GGTATCTCAC CCCGGACCAG GATCCCGACG TACTGCTGGA CAAGCTCAGC TCGTACGTGG GATTCACTCC GGTGCACAAC GCCTCCGGTG CGCCAGCGAT ATCGCTGCCG CTCGGCCAAG ACCCCGACGG CCTGCCCATC GGAGTGATGT TCAGCGGCCG CCGAGGCAGT GAACGAACGT TGCTCGAGCT GGCGATGGAA ATCGAGGAGG CACAGCCCTT CTCGATACTG GGC
Details for MyabA.00951.b.A1.GE30379
HARVESTED ON: 9/8/2010
SEQUENCED ON: 9/17/2010
EXPECTED MW: 52kDa
OBSERVED MW: 52kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 4
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTR
Validated NT Sequence
ttttgtttac tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgacccg ctgagcaata actannnnng acgccctggg cgacctggat gccaccggan tcnccnagcg aatcgcctcc ggcgaggtcn ctgcnncnaa tgcnccgagg ccgccatcnn nngtctcnag cgggtcannn nnacnctcaa nnnnnnggaa ttcaaggatt ttccgcgcgc ccgcgagcgc gccgaacagg cagacgccgc gtccagcact tccggcactt ttcggggtgt gcccaccgcg acgaaatgca atatccacgt ggcgggtatg ccactgacgg aaggctcggc ggcgatcacg ccggcaccgc agactgacga tggaccgttc gcacgacaat tcctcagcac cggcgtgatc cccatcgcct cgacgaccat gcccgaatac ggctggtctg ccaccacgga gcgcgcaggt ggctcggcta cccgcaatcc gtggcatacg gacttctctg ccggcggatc ctccggcggg tcggcggcac tggtagccgc cggtgtggtg cccatcgcac acggcaatga cggcggcggc tcgatccgga tccccgcggc ggcctgcgga ctcgtcgncc tcaagccgag ccgcggaagg cttttgggcg acccgtcnag cagttcggcc ccagtgaaaa tcgtcgccga gggggtgctg gtgcgcagtg tgcgnnantg tgcccgcttc ttcgagtcgg ccgaactgca ctatcgcaac ccaaagttga caccgatcgg gnacgtggat cgcccggtcc agcgccngct gcgcatcggn ctatgcatgn nntccccctt cactcccgcc accgacaacc caacccggca ggcagtgaac tccgncgcnn nttgctngnn tcccttggcc acnccnntcg aacntacann cccngccnng cttncgncnn ncaanatcna cttnnangac ngggnntnnn nnnnnnncnn nnggntnnnn ngnccccnnn ngntcnangg nnncganccn nctannnnnn nnnnnnntng gnnnnnnnnn nagnnnnnnn nncnnnnncc nnannnnnnt nncanngnnn nnnaannnnn ng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTRIHAFTE DALGDLDATG VAERIASGEV TAAECAEAAI VRLERVNPTL NAVEFKDFPR ARERAEQADA ASSTSGTFRG VPTATKCNIH VAGMPLTEGS AAITPAPQTD DGPFARQFLS TGVIPIASTT MPEYGWSATT ERAGGSATRN PWHTDFSAGG SSGGSAALVA AGVVPIAHGN DGGGSIRIPA AACGLVGLKP SRGRLLGDPS SSSAPVKIVA EGVLVRSVRD CARFFESAEL HYRNPKLTPI GHVDRPVQRR LRIGLCMDSP FTPATDNPTR QAVNSAAELL ESLGHTVEPY TPAVLPSFKI DFEDYWAFLA FAVAKGGPRL FEGFDPAKLD RLTLGLSRRF VRRSYRTPLF LARLAAAARV YERGFTTGID AVLTPVLTHT TPRIGYLTPD QDPDVLLDKL SSYVGFTPVH NASGAPAISL PLGQDPDGLP IGVMFSGRRG SERTLLELAM EIEEAQPFSI LG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacccgcatc cacgcgttta ccgaggacgc cctgggcgac ctggatgcca ccggagtcgc cgagcgaatc gcctccggcg aggtcactgc cgccgaatgc gccgaggccg ccatcgtccg tctcgagcgg gtcaacccga cgctcaacgc agtggaattc aaggattttc cgcgcgcccg cgagcgcgcc gaacaggcag acgccgcgtc cagcacttcc ggcacttttc ggggtgtgcc caccgcgacg aaatgcaata tccacgtggc gggtatgcca ctgacggaag gctcggcggc gatcacgccg gcaccgcaga ctgacgatgg accgttcgca cgacaattcc tcagcaccgg cgtgatcccc atcgcctcga cgaccatgcc cgaatacggc tggtctgcca ccacggagcg cgcaggtggc tcggctaccc gcaatccgtg gcatacggac ttctctgccg gcggatcctc cggcgggtcg gcggcactgg tagccgccgg tgtggtgccc atcgcacacg gcaatgacgg cggcggctcg atccggatcc ccgcggcggc ctgcggactc gtcggcctca agccgagccg cggaaggctt ttgggcgacc cgtcgagcag ttcggcccca gtgaaaatcg tcgccgaggg ggtgctggtg cgcagtgtgc gggactgtgc ccgcttcttc gagtcggccg aactgcacta tcgcaaccca aagttgacac cgatcgggca cgtggatcgc ccggtccagc gccggctgcg catcggccta tgcatggatt cccccttcac tcccgccacc gacaacccaa cccggcaggc agtgaactcc gccgcggaat tgcttgaatc ccttggccac accgtcgaac cgtacacacc cgccgtgctt ccgtcgttca agatcgactt cgaggactac tgggccttct tggccttcgc cgtggctaag ggtggtcccc ggctgttcga ggggttcgac cccgctaagt tggaccggct gaccctcggg ctcagccgca gattcgtcag gcgctcgtac cgcacgccgc tcttcttggc acgcctggcc gccgccgccc gggtatacga acgcggcttc accaccggta tcgatgcggt gctcacgccc gtgctcaccc acaccacgcc acgcatcggg tatctcaccc cggaccagga tcccgacgta ctgctggaca agctcagctc gtacgtggga ttcactccgg tgcacaacgc ctccggtgcg ccagcgatat cgctgccgct cggccaagac cccgacggcc tgcccatcgg agtgatgttc agcggccgcc gaggcagtga acgaacgttg ctcgagctgg cgatggaaat cgaggaggca cagcccttct cgatactggg ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa