MyabA.00951.a

Putative amidase

CENTER ID: MyabA.00951.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00951.a.A1.GE30358 Full length( MyabA.00951.a ) 1 491
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.2220
RefSeq: YP_001702916.1
UniProt: B1MAJ1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDFSDYSQHD ATGLAELVAT KQVTPAELLA AARARAADVN NKIGAIVRTL PEADQQVQGD LTGPFAGVPF LIKDLGQDYK GHPTSGGCRA LSTLPAPEHS TIVRRWLDAG LVIFGKTNTP EFGSKGITEP ELFGASRNPW DLGRTPGGSS GGSAAAVAAG IVPVAGASDG GGSIRIPAGC CGLVGLKPGR GITPMGPTAG EPMHGAAVNG TVSRTVRDTA SMLDIIAGPE PTSPYLSAMP QDSYASQVGR DPGRLRIGVR VPTVITPNPD PAAVAAVETA IRLLTHLGHE VEEVSAPYDD GQLARDFLLT WFVHAAYEVD YTKRLTGAGD EGFERDTLLI AALGRTTSGP EYVAAVERRH DYVRELATFF GDYDLLLTPT LAKAPPRIGE FNLPAPLAAV SDLLLRTKTA RVLKYANIVD SMINDNLGWV PYTQLANLTG RPAISLPLYQ TPKGLPLGVQ FVAPLGGESL LIRLAAQLEA TEPWAHRRPT L
NT Sequence
GTGGATTTCT CCGACTACTC CCAACATGAC GCCACCGGCC TGGCCGAGCT GGTCGCCACC AAACAGGTCA CCCCAGCCGA GCTGCTGGCC GCGGCCCGCG CTCGCGCTGC CGACGTCAAC AACAAGATCG GTGCGATAGT CCGAACTCTC CCCGAAGCCG ACCAGCAAGT GCAGGGCGAC CTGACCGGGC CGTTCGCCGG GGTGCCGTTT CTCATCAAGG ACCTCGGCCA GGACTACAAG GGCCATCCCA CATCGGGAGG ATGCCGGGCG TTGTCCACAC TCCCCGCGCC GGAGCACTCC ACCATCGTGC GACGCTGGCT CGATGCCGGC CTGGTCATCT TCGGCAAGAC CAACACCCCG GAGTTCGGGT CCAAGGGCAT CACCGAGCCG GAATTGTTTG GGGCGAGCCG TAACCCGTGG GACCTAGGGC GCACACCCGG CGGCTCCTCG GGCGGCTCGG CCGCCGCGGT CGCCGCCGGA ATCGTGCCGG TGGCGGGAGC CAGCGATGGC GGCGGATCGA TCCGGATCCC CGCCGGATGT TGCGGCCTGG TTGGGCTCAA GCCCGGCCGC GGTATCACCC CGATGGGTCC CACAGCCGGC GAGCCGATGC ATGGCGCGGC GGTCAACGGC ACCGTCTCCC GAACCGTCCG CGATACCGCG TCCATGCTCG ATATCATCGC CGGGCCCGAG CCCACCTCGC CGTATTTGTC TGCGATGCCC CAGGATTCGT ACGCATCGCA AGTAGGACGT GATCCTGGCA GGCTCCGTAT CGGGGTGCGC GTGCCAACCG TGATCACTCC GAACCCCGAT CCCGCCGCGG TGGCCGCGGT CGAGACCGCC ATCCGTCTGC TGACCCATCT GGGGCACGAG GTAGAGGAGG TGAGCGCACC CTATGACGAT GGTCAGCTCG CCCGCGACTT CTTGTTGACG TGGTTCGTGC ATGCCGCCTA CGAGGTGGAC TACACCAAGC GGCTGACCGG AGCGGGAGAC GAGGGGTTTG AGCGCGACAC GCTCCTCATC GCGGCATTAG GACGCACCAC CAGCGGACCC GAATATGTTG CGGCCGTGGA ACGCCGACAC GACTACGTAC GGGAGCTGGC GACCTTCTTC GGCGACTACG ATCTGCTGCT CACGCCGACA CTCGCGAAGG CGCCGCCGCG GATCGGCGAG TTCAATCTCC CCGCGCCACT CGCCGCCGTG TCTGATCTGT TGCTGCGCAC CAAGACCGCC CGCGTCCTCA AATACGCGAA CATTGTCGAC AGCATGATCA ACGACAACCT CGGCTGGGTC CCCTACACCC AGTTGGCGAA TCTGACAGGG CGCCCAGCGA TCTCATTGCC GCTCTATCAG ACACCGAAGG GGCTGCCCCT CGGTGTGCAG TTTGTCGCAC CGCTCGGCGG CGAATCACTC CTGATTCGGC TGGCTGCCCA GCTCGAGGCA ACCGAACCGT GGGCACACCG GCGCCCAACC CTA
Details for MyabA.00951.a.A1.GE30358
HARVESTED ON: 9/8/2010
SEQUENCED ON: 9/17/2010
EXPECTED MW: 54kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 55
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVDFSDYSQ HDATGLAELV ATKQVTPAEL LAAARARAAD VNNKIGAIVR TLPEADQQVQ GDLTGPFAGV PFLIKDLGQD YKGHPTSGGC RALSTLPAPE HSTIVRRWLD AGLVIFGKTN TPEFGSKGIT EPELFGASRN PWDLGRTPGG SSGGSAAAVA AGIVPVAGAS DGGGSIRIPA GCCGLVGLKP GRGITPMGPT AGEPMHGAAV NGTVSRTVRD TASMLDIIAX PEPTSPYLSA MPXDSYXSQV XRDPGXXRIG VRVPP
Validated NT Sequence
tttgtttant ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggtggat ttctccgact actcccaaca tgacgccacc ggcctggccg agctggtcgc caccaaacag gtcaccccag ccgagctgct ggccgcggcc cgcgctcgcg ctgccgacgt caacaacaag atcggtgcga tagtccgaac tctccccgaa gccgaccagc aagtgcaggg cgacctgacc gggccgttcg ccggggtgcc gtttctcatc aaggacctcg gccaggacta caagggccat cccacatcgg gaggatgccg ggcgttgtcc acactccccg cgccggagca ctccaccatc gtgcgacgct ggctcgatgc cggcctggtc atcttcggca agaccaacac cccggagttc gggtccaagg gcatcaccga gccggaattg tttggggcga gccgtaaccc gtgggaccta gggcgcacac ccggcggctc ctcgggcggc tcggccgccg cggtcgccgc cggaatcgtg ccggtggcgg gagccagcga tggcggcgga tcgatccgga tccccgccgg atgttgcggc ctggttgggc tcaagcccgg ccgcggtatc accccgatgg gtcccacagc cggcgagccg atgcatggcg cggcggtcaa cggcaccgtc tcccgaaccg tccgcgatac cgcgtccatg ctcgatatca tcgccnggcc cgagcccacc tcgccgtatt tgtctgcgat gccnngngat tcgtacncat cncaagtang acgtgatcct ggcangntcc gtatcggggt gcgcgtgcca ccgtgatcac tccnaacccc natcccgcnn cggtggccgc ggtcnagacc gccatccgtc tgctgannca tctggggnan ganntagang aggngagcca ccctatgacg atggtcancn nnncnnnnnn ttcntnnnna nntgntgcat gnnnnctnnn nnnnnnnnnn ccnannngnc tgannnnagc nggnannann nnnnnnannn nnnncnnnnn cntcnnnnnn nnnntnngna ncnnncnnnn nnnnnnnnnn ntnnngnnnn nnnnnnnnnc nnnnnanntc nnnnnnnnnn nnnannnnnn nngnnannnn nngna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVDFSDYSQ HDATGLAELV ATKQVTPAEL LAAARARAAD VNNKIGAIVR TLPEADQQVQ GDLTGPFAGV PFLIKDLGQD YKGHPTSGGC RALSTLPAPE HSTIVRRWLD AGLVIFGKTN TPEFGSKGIT EPELFGASRN PWDLGRTPGG SSGGSAAAVA AGIVPVAGAS DGGGSIRIPA GCCGLVGLKP GRGITPMGPT AGEPMHGAAV NGTVSRTVRD TASMLDIIAG PEPTSPYLSA MPQDSYASQV GRDPGRLRIG VRVPTVITPN PDPAAVAAVE TAIRLLTHLG HEVEEVSAPY DDGQLARDFL LTWFVHAAYE VDYTKRLTGA GDEGFERDTL LIAALGRTTS GPEYVAAVER RHDYVRELAT FFGDYDLLLT PTLAKAPPRI GEFNLPAPLA AVSDLLLRTK TARVLKYANI VDSMINDNLG WVPYTQLANL TGRPAISLPL YQTPKGLPLG VQFVAPLGGE SLLIRLAAQL EATEPWAHRR PTL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggatttc tccgactact cccaacatga cgccaccggc ctggccgagc tggtcgccac caaacaggtc accccagccg agctgctggc cgcggcccgc gctcgcgctg ccgacgtcaa caacaagatc ggtgcgatag tccgaactct ccccgaagcc gaccagcaag tgcagggcga cctgaccggg ccgttcgccg gggtgccgtt tctcatcaag gacctcggcc aggactacaa gggccatccc acatcgggag gatgccgggc gttgtccaca ctccccgcgc cggagcactc caccatcgtg cgacgctggc tcgatgccgg cctggtcatc ttcggcaaga ccaacacccc ggagttcggg tccaagggca tcaccgagcc ggaattgttt ggggcgagcc gtaacccgtg ggacctaggg cgcacacccg gcggctcctc gggcggctcg gccgccgcgg tcgccgccgg aatcgtgccg gtggcgggag ccagcgatgg cggcggatcg atccggatcc ccgccggatg ttgcggcctg gttgggctca agcccggccg cggtatcacc ccgatgggtc ccacagccgg cgagccgatg catggcgcgg cggtcaacgg caccgtctcc cgaaccgtcc gcgataccgc gtccatgctc gatatcatcg ccgggcccga gcccacctcg ccgtatttgt ctgcgatgcc ccaggattcg tacgcatcgc aagtaggacg tgatcctggc aggctccgta tcggggtgcg cgtgccaacc gtgatcactc cgaaccccga tcccgccgcg gtggccgcgg tcgagaccgc catccgtctg ctgacccatc tggggcacga ggtagaggag gtgagcgcac cctatgacga tggtcagctc gcccgcgact tcttgttgac gtggttcgtg catgccgcct acgaggtgga ctacaccaag cggctgaccg gagcgggaga cgaggggttt gagcgcgaca cgctcctcat cgcggcatta ggacgcacca ccagcggacc cgaatatgtt gcggccgtgg aacgccgaca cgactacgta cgggagctgg cgaccttctt cggcgactac gatctgctgc tcacgccgac actcgcgaag gcgccgccgc ggatcggcga gttcaatctc cccgcgccac tcgccgccgt gtctgatctg ttgctgcgca ccaagaccgc ccgcgtcctc aaatacgcga acattgtcga cagcatgatc aacgacaacc tcggctgggt cccctacacc cagttggcga atctgacagg gcgcccagcg atctcattgc cgctctatca gacaccgaag gggctgcccc tcggtgtgca gtttgtcgca ccgctcggcg gcgaatcact cctgattcgg ctggctgccc agctcgaggc aaccgaaccg tgggcacacc ggcgcccaac cctataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa