MyabA.00950.b

Probable lipoprotein aminopeptidase LpqL

CENTER ID: MyabA.00950.b
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00950.b.A2.GE30357 Mature protein( MyabA.00950.b ) 23 500
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.4292
RefSeq: YP_001704940.1
UniProt: B1MIR5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MATNRVLYAV AVSAACALAT VTACDRDAAE APPRSAPQAG SEEAVGFAHS LHEKVTVDNV VKHLSALQEI ADKNNNTRAA GTAGFDQSVD YVVKALKDKG FDVQTPEFSF KYFQAKSLDL TVGPKKVDAG VLSYSPGGRV EGRLVPARAE ESPGCTVEDY DGLDVKGAVV LVDRGSCPFA DKERVAAERG AVAVIIADNV DENKTSGTLG EDSNPKIPVV SVTKSVGADL RAHPDKVVLN VDAETKDVKA RNVIAQTKTG ATTDVVMAGA HLDSVPEGPG INDNGTGTAA VLETALQLGP SPDVKNAVRF AFWGAEEEGL IGSTDYVKSL DVAALKNIAL YLNYDMLGSP NAAYLTYDGD QSNEPDPNEI PVRIPEGSAG IERTEVAYLA EQGKKAHDTG YDGRSDYDAF SRAGIPTGGI FSGAEDKMSD EEAKQWGGKA GQPFDPNYHQ AGDTLANVNK DALKINAGGV AYTVGLYAQS IDGRNGVPVH EDRTRHQLKG
NT Sequence
ATGGCGACCA ATCGGGTTCT GTATGCGGTC GCGGTGTCCG CGGCGTGCGC GCTGGCAACG GTCACGGCGT GCGACCGTGA TGCCGCGGAG GCGCCGCCTC GCTCGGCGCC TCAGGCAGGA TCCGAAGAAG CCGTCGGTTT CGCGCACTCC CTGCATGAGA AGGTGACCGT CGACAACGTC GTGAAGCATC TGTCGGCCCT CCAGGAAATC GCCGACAAGA ACAACAACAC CAGGGCGGCG GGGACCGCCG GATTTGACCA GAGCGTCGAC TACGTGGTGA AGGCTTTGAA GGACAAGGGG TTCGATGTGC AAACCCCGGA ATTCAGCTTC AAATACTTCC AGGCCAAGTC CCTTGATCTG ACGGTCGGAC CCAAGAAGGT GGACGCAGGG GTGCTGTCCT ACTCGCCGGG TGGTCGCGTC GAGGGACGGC TGGTGCCGGC CCGCGCGGAG GAGTCGCCCG GTTGTACGGT CGAGGATTAC GACGGGCTTG ACGTCAAGGG CGCAGTGGTG CTTGTCGACC GCGGATCATG CCCATTCGCC GACAAGGAAA GGGTCGCCGC CGAACGCGGC GCGGTCGCCG TGATCATCGC GGACAACGTC GACGAGAATA AGACGAGCGG CACCCTTGGT GAGGACTCCA ACCCCAAGAT CCCGGTGGTG AGTGTTACGA AATCGGTCGG TGCGGACCTG AGGGCCCATC CGGACAAGGT TGTCCTCAAT GTCGATGCGG AGACCAAGGA CGTCAAGGCG CGCAACGTGA TCGCGCAGAC CAAGACGGGC GCCACGACAG ATGTCGTCAT GGCAGGCGCG CATCTGGACA GTGTTCCGGA GGGGCCGGGA ATCAACGACA ATGGCACCGG CACCGCCGCG GTGCTGGAGA CGGCGCTGCA ACTGGGGCCG AGCCCCGATG TGAAGAACGC GGTGCGATTC GCGTTCTGGG GCGCGGAGGA AGAAGGGCTG ATCGGCTCCA CCGACTACGT CAAATCGCTT GATGTGGCTG CGCTGAAAAA CATTGCCCTG TACTTGAATT ACGACATGCT CGGTTCGCCG AACGCCGCGT ACCTGACGTA CGACGGCGAT CAGTCTAACG AGCCCGACCC GAACGAGATT CCGGTGCGGA TTCCCGAGGG CTCGGCAGGT ATCGAGCGAA CCGAGGTCGC CTATCTGGCC GAGCAGGGCA AGAAGGCCCA TGACACCGGC TATGACGGCC GCTCGGACTA TGACGCCTTC AGCCGCGCCG GCATTCCGAC CGGCGGCATT TTCTCTGGCG CCGAAGACAA GATGTCGGAC GAAGAGGCCA AACAATGGGG CGGTAAGGCG GGGCAGCCGT TCGATCCCAA TTACCACCAG GCGGGCGACA CGCTGGCCAA CGTGAACAAG GATGCGCTCA AGATCAACGC GGGCGGAGTG GCCTACACCG TCGGGTTGTA CGCGCAGAGC ATTGACGGAC GCAACGGTGT TCCCGTTCAT GAGGACCGCA CCCGCCACCA GCTCAAGGGG
Details for MyabA.00950.b.A2.GE30357
HARVESTED ON: 9/8/2010
SEQUENCED ON: 9/17/2010
EXPECTED MW: 52kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 82
PERCENT COVERAGE: 76
Validated AA Sequence
MAHHHHHHMG TLEAQTXGPG SMACDRDAAE APPRSAPQAG SEEAVGFAHS LHEKVTVDNV VKHLSALQEI ADKNNNTRAA GTAGFDQSVD YVVKALKDKG FDVQTPEFSF KYFQAKSLDL TVGPKKVDAG VLSYSPGGRV EGRLVPARAE ESPGCTVEDY DGLDVKGAVV LVDRGSCPFA DKERVAAERG AVAVIIADNV DENKTSGTLG EDSNPKIPVV SVTKSVGADL XAHPDKVVLN VDAETKDVKA RNVIAQTKTG ATTDVVMAGA HLDSVPEGPG INDNGTGTAA VLEXALQLGP SPXVXXAXRX XFWGAXIXSX DYXNRXXXLR XKXXXXXXXX HXXXXXXLXX YXXIXLXXXX XXXXPXXXXX XXXXXXXXXX
Validated NT Sequence
tnnnnntttg tttactttaa gaaggagata taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccanggncc tggttcgatg gcgtgcgacc gtgatgccgc ggaggcgccg cctcgctcgg cgcctcaggc aggatccgaa gaagccgtcg gtttcgcgca ctccctgcat gagaaggtga ccgtcgacaa cgtcgtgaag catctgtcgg ccctccagga aatcgccgac aagaacaaca acaccagggc ggcggggacc gccggatttg accagagcgt cgactacgtg gtgaaggctt tgaaggacaa ggggttcgat gtgcaaaccc cggaattcag cttcaaatac ttccaggcca agtcccttga tctgacggtc ggacccaaga aggtggacgc aggggtgctg tcctactcgc cgggtggtcg cgtcgaggga cggctggtgc cggcccgcgc ggaggagtcg cccggttgta cggtcgagga ttacgacggg cttgacgtca agggcgcagt ggtgcttgtc gaccgcggat catgcccatt cgccgacaag gaaagggtcg ccgccgaacg cggcgcggtc gccgtgatca tcgcggacaa cgtcgacgag aataagacga gcggcaccct tggtgaggac tccaacccca agatcccggt ggtgagtgtt acgaaatcgg tcggtgcgga cctganggcc catccggaca aggttgtcct caatgtcgat gcggagacca aggacgtcaa ggcgcgcaac gtgatcgcgc agaccaagac gggcgccacg acagatgtcg tcatggcagg cgcgcatctg gacagtgttc cggaggggcc gggaatcaac gacaatggca ccggcaccgc cgcggtgctg gaganngcgc tgcaactggg gccgagcccc natgtganna angcggngcg attnncgttc tggggcgcgn tgatcngctc cnccgactac ntcaatcgcn tgnngtngct gcgctnaaaa nattgncnng nnnntnannt acnacatgnt cngnncnnnn aangnctann nanntacgan gnnatcantc tnnnnncnnn anccnnnngn ncnnnnccnn nnnnnnnnnn nnnnannnnn nnnnnannnn nnnnnnnnnn nnnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMACDRDAAE APPRSAPQAG SEEAVGFAHS LHEKVTVDNV VKHLSALQEI ADKNNNTRAA GTAGFDQSVD YVVKALKDKG FDVQTPEFSF KYFQAKSLDL TVGPKKVDAG VLSYSPGGRV EGRLVPARAE ESPGCTVEDY DGLDVKGAVV LVDRGSCPFA DKERVAAERG AVAVIIADNV DENKTSGTLG EDSNPKIPVV SVTKSVGADL RAHPDKVVLN VDAETKDVKA RNVIAQTKTG ATTDVVMAGA HLDSVPEGPG INDNGTGTAA VLETALQLGP SPDVKNAVRF AFWGAEEEGL IGSTDYVKSL DVAALKNIAL YLNYDMLGSP NAAYLTYDGD QSNEPDPNEI PVRIPEGSAG IERTEVAYLA EQGKKAHDTG YDGRSDYDAF SRAGIPTGGI FSGAEDKMSD EEAKQWGGKA GQPFDPNYHQ AGDTLANVNK DALKINAGGV AYTVGLYAQS IDGRNGVPVH EDRTRHQLKG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgtgcgac cgtgatgccg cggaggcgcc gcctcgctcg gcgcctcagg caggatccga agaagccgtc ggtttcgcgc actccctgca tgagaaggtg accgtcgaca acgtcgtgaa gcatctgtcg gccctccagg aaatcgccga caagaacaac aacaccaggg cggcggggac cgccggattt gaccagagcg tcgactacgt ggtgaaggct ttgaaggaca aggggttcga tgtgcaaacc ccggaattca gcttcaaata cttccaggcc aagtcccttg atctgacggt cggacccaag aaggtggacg caggggtgct gtcctactcg ccgggtggtc gcgtcgaggg acggctggtg ccggcccgcg cggaggagtc gcccggttgt acggtcgagg attacgacgg gcttgacgtc aagggcgcag tggtgcttgt cgaccgcgga tcatgcccat tcgccgacaa ggaaagggtc gccgccgaac gcggcgcggt cgccgtgatc atcgcggaca acgtcgacga gaataagacg agcggcaccc ttggtgagga ctccaacccc aagatcccgg tggtgagtgt tacgaaatcg gtcggtgcgg acctgagggc ccatccggac aaggttgtcc tcaatgtcga tgcggagacc aaggacgtca aggcgcgcaa cgtgatcgcg cagaccaaga cgggcgccac gacagatgtc gtcatggcag gcgcgcatct ggacagtgtt ccggaggggc cgggaatcaa cgacaatggc accggcaccg ccgcggtgct ggagacggcg ctgcaactgg ggccgagccc cgatgtgaag aacgcggtgc gattcgcgtt ctggggcgcg gaggaagaag ggctgatcgg ctccaccgac tacgtcaaat cgcttgatgt ggctgcgctg aaaaacattg ccctgtactt gaattacgac atgctcggtt cgccgaacgc cgcgtacctg acgtacgacg gcgatcagtc taacgagccc gacccgaacg agattccggt gcggattccc gagggctcgg caggtatcga gcgaaccgag gtcgcctatc tggccgagca gggcaagaag gcccatgaca ccggctatga cggccgctcg gactatgacg ccttcagccg cgccggcatt ccgaccggcg gcattttctc tggcgccgaa gacaagatgt cggacgaaga ggccaaacaa tggggcggta aggcggggca gccgttcgat cccaattacc accaggcggg cgacacgctg gccaacgtga acaaggatgc gctcaagatc aacgcgggcg gagtggccta caccgtcggg ttgtacgcgc agagcattga cggacgcaac ggtgttcccg ttcatgagga ccgcacccgc caccagctca aggggtaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa