MyabA.00937.a
tRNA (guanine-N(1)-)-methyltransferase
| CENTER ID: | MyabA.00937.a |
| ORGANISM: | Mycobacterium abscessus ATCC 19977 / DSM 44196 |
| ASSOCIATED DISEASE: | Skin abscess |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyabA.00937.a.A1.GE30355 | Full length( MyabA.00937.a ) | 1 | 242 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyabA.00937.a.A1.PW30232 | Full length( MyabA.00937.a ) | 1 | 242 |
Structures
3QUV
DEPOSITED: 2/24/2011
DETERMINATION: XRay
CLONE: MyabA.00937.a.A1.GE30355
PROTEIN: MyabA.00937.a.A1.PW30232
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|36809.5.peg.3284 |
| RefSeq: | YP_001703956.1 |
| UniProt: | B1MDI3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKIDVVTIFP EYLQPVRQSL PGKAIDAGLV DVAVHDLRRW THDVHKSVDD SPYGGGPGMV MKPTVWGDAL DEICTSETLL VVPTPAGYPF TQETAWQWST EDHLVIACGR YEGIDQRVAD DAATRMRVRE VSIGDYVLNG GEAAALVIIE AVLRLVPGVL GNALSAQEDS HSEGMASLLE GPSYTRPPSW RGMDVPPVLL SGDHAKIAAW RAEQSRQRTI ERRPDLLGFD SPTGEHGGDG LS
NT Sequence
ATGAAAATAG ACGTCGTCAC GATCTTCCCG GAATATCTGC AACCCGTGCG GCAGTCCTTG CCGGGCAAGG CTATTGACGC TGGTTTGGTC GATGTCGCGG TGCATGATCT GCGGCGCTGG ACGCACGACG TGCACAAGTC CGTCGATGAC TCGCCATACG GTGGTGGGCC CGGCATGGTG ATGAAGCCGA CGGTCTGGGG CGATGCGCTG GATGAGATCT GCACATCTGA AACGCTTTTG GTGGTGCCGA CACCCGCTGG CTACCCGTTC ACCCAGGAGA CCGCCTGGCA GTGGTCCACC GAAGATCATC TGGTGATCGC ATGTGGGCGG TACGAGGGAA TCGACCAGCG CGTCGCCGAC GACGCCGCGA CACGGATGCG GGTGCGCGAG GTGTCGATCG GTGACTACGT ACTCAACGGC GGCGAGGCCG CTGCTCTGGT GATCATCGAA GCGGTGTTGC GTCTGGTTCC CGGTGTGCTG GGCAACGCGC TTTCCGCACA GGAAGATTCA CACTCCGAGG GAATGGCCAG CCTCTTGGAG GGGCCGTCGT ACACCAGACC GCCGAGCTGG CGGGGGATGG ATGTGCCACC GGTGCTGCTC TCGGGAGATC ACGCCAAGAT CGCGGCCTGG CGGGCCGAAC AGTCGCGTCA GCGCACTATT GAGCGGCGCC CAGATCTACT CGGTTTCGAT TCGCCCACCG GGGAACATGG TGGCGACGGC CTGAGT
Details for MyabA.00937.a.A1.GE30355
| HARVESTED ON: | 9/8/2010 |
| SEQUENCED ON: | 7/10/2015 |
| EXPECTED MW: | 28kDa |
| OBSERVED MW: | 29kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKIDVVTIF PEYLQPVRQS LPGKAIDAGL VDVAVHDLRR WTHDVHKSVD DSPYGGGPGM VMKPTVWGDA LDEICTSETL LVVPTPAGYP FTQETAWQWS TEDHLVIACG RYEGIDQRVA DDAATRMRVR EVSIGDYVLN GGEAAALVII EAVLRLVPGV LGNALSAQED SHSEGMASLL EGPSYTRPPS WRGMDVPPVL LSGDHAKIAA WRAEQSRQRT IERRPDLLGF DSPTGEHGGD GLS
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaaa tagacgtcgt cacgatcttc ccggaatatc tgcaacccgt gcggcagtcc ttgccgggca aggctattga cgctggtttg gtcgatgtcg cggtgcatga tctgcggcgc tggacgcacg acgtgcacaa gtccgtcgat gactcgccat acggtggtgg gcccggcatg gtgatgaagc cgacggtctg gggcgatgcg ctggatgaga tctgcacatc tgaaacgctt ttggtggtgc cgacacccgc tggctacccg ttcacccagg agaccgcctg gcagtggtcc accgaagatc atctggtgat cgcatgtggg cggtacgagg gaatcgacca gcgcgtcgcc gacgacgccg cgacacggat gcgggtgcgc gaggtgtcga tcggtgacta cgtactcaac ggcggcgagg ccgctgctct ggtgatcatc gaagcggtgt tgcgtctggt tcccggtgtg ctgggcaacg cgctttccgc acaggaagat tcacactccg agggaatggc cagcctcttg gaggggccgt cgtacaccag accgccgagc tggcggggga tggatgtgcc accggtgctg ctctcgggag atcacgccaa gatcgcggcc tggcgggccg aacagtcgcg tcagcgcact attgagcggc gcccagatct actcggtttc gattcgccca ccggggaaca tggtggcgac ggcctgagtt aataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKIDVVTIF PEYLQPVRQS LPGKAIDAGL VDVAVHDLRR WTHDVHKSVD DSPYGGGPGM VMKPTVWGDA LDEICTSETL LVVPTPAGYP FTQETAWQWS TEDHLVIACG RYEGIDQRVA DDAATRMRVR EVSIGDYVLN GGEAAALVII EAVLRLVPGV LGNALSAQED SHSEGMASLL EGPSYTRPPS WRGMDVPPVL LSGDHAKIAA WRAEQSRQRT IERRPDLLGF DSPTGEHGGD GLS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaaatagac gtcgtcacga tcttcccgga atatctgcaa cccgtgcggc agtccttgcc gggcaaggct attgacgctg gtttggtcga tgtcgcggtg catgatctgc ggcgctggac gcacgacgtg cacaagtccg tcgatgactc gccatacggt ggtgggcccg gcatggtgat gaagccgacg gtctggggcg atgcgctgga tgagatctgc acatctgaaa cgcttttggt ggtgccgaca cccgctggct acccgttcac ccaggagacc gcctggcagt ggtccaccga agatcatctg gtgatcgcat gtgggcggta cgagggaatc gaccagcgcg tcgccgacga cgccgcgaca cggatgcggg tgcgcgaggt gtcgatcggt gactacgtac tcaacggcgg cgaggccgct gctctggtga tcatcgaagc ggtgttgcgt ctggttcccg gtgtgctggg caacgcgctt tccgcacagg aagattcaca ctccgaggga atggccagcc tcttggaggg gccgtcgtac accagaccgc cgagctggcg ggggatggat gtgccaccgg tgctgctctc gggagatcac gccaagatcg cggcctggcg ggccgaacag tcgcgtcagc gcactattga gcggcgccca gatctactcg gtttcgattc gcccaccggg gaacatggtg gcgacggcct gagttaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for MyabA.00937.a.A1.PW30232
| PURIFICATION DATe: | 11/29/2010 |
| CONCENTRATION: | 24.41mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKIDVVTIF PEYLQPVRQS LPGKAIDAGL VDVAVHDLRR WTHDVHKSVD DSPYGGGPGM VMKPTVWGDA LDEICTSETL LVVPTPAGYP FTQETAWQWS TEDHLVIACG RYEGIDQRVA DDAATRMRVR EVSIGDYVLN GGEAAALVII EAVLRLVPGV LGNALSAQED SHSEGMASLL EGPSYTRPPS WRGMDVPPVL LSGDHAKIAA WRAEQSRQRT IERRPDLLGF DSPTGEHGGD GLS
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaaa tagacgtcgt cacgatcttc ccggaatatc tgcaacccgt gcggcagtcc ttgccgggca aggctattga cgctggtttg gtcgatgtcg cggtgcatga tctgcggcgc tggacgcacg acgtgcacaa gtccgtcgat gactcgccat acggtggtgg gcccggcatg gtgatgaagc cgacggtctg gggcgatgcg ctggatgaga tctgcacatc tgaaacgctt ttggtggtgc cgacacccgc tggctacccg ttcacccagg agaccgcctg gcagtggtcc accgaagatc atctggtgat cgcatgtggg cggtacgagg gaatcgacca gcgcgtcgcc gacgacgccg cgacacggat gcgggtgcgc gaggtgtcga tcggtgacta cgtactcaac ggcggcgagg ccgctgctct ggtgatcatc gaagcggtgt tgcgtctggt tcccggtgtg ctgggcaacg cgctttccgc acaggaagat tcacactccg agggaatggc cagcctcttg gaggggccgt cgtacaccag accgccgagc tggcggggga tggatgtgcc accggtgctg ctctcgggag atcacgccaa gatcgcggcc tggcgggccg aacagtcgcg tcagcgcact attgagcggc gcccagatct actcggtttc gattcgccca ccggggaaca tggtggcgac ggcctgagtt aataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKIDVVTIF PEYLQPVRQS LPGKAIDAGL VDVAVHDLRR WTHDVHKSVD DSPYGGGPGM VMKPTVWGDA LDEICTSETL LVVPTPAGYP FTQETAWQWS TEDHLVIACG RYEGIDQRVA DDAATRMRVR EVSIGDYVLN GGEAAALVII EAVLRLVPGV LGNALSAQED SHSEGMASLL EGPSYTRPPS WRGMDVPPVL LSGDHAKIAA WRAEQSRQRT IERRPDLLGF DSPTGEHGGD GLS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAAATAGAC GTCGTCACGA TCTTCCCGGA ATATCTGCAA CCCGTGCGGC AGTCCTTGCC GGGCAAGGCT ATTGACGCTG GTTTGGTCGA TGTCGCGGTG CATGATCTGC GGCGCTGGAC GCACGACGTG CACAAGTCCG TCGATGACTC GCCATACGGT GGTGGGCCCG GCATGGTGAT GAAGCCGACG GTCTGGGGCG ATGCGCTGGA TGAGATCTGC ACATCTGAAA CGCTTTTGGT GGTGCCGACA CCCGCTGGCT ACCCGTTCAC CCAGGAGACC GCCTGGCAGT GGTCCACCGA AGATCATCTG GTGATCGCAT GTGGGCGGTA CGAGGGAATC GACCAGCGCG TCGCCGACGA CGCCGCGACA CGGATGCGGG TGCGCGAGGT GTCGATCGGT GACTACGTAC TCAACGGCGG CGAGGCCGCT GCTCTGGTGA TCATCGAAGC GGTGTTGCGT CTGGTTCCCG GTGTGCTGGG CAACGCGCTT TCCGCACAGG AAGATTCACA CTCCGAGGGA ATGGCCAGCC TCTTGGAGGG GCCGTCGTAC ACCAGACCGC CGAGCTGGCG GGGGATGGAT GTGCCACCGG TGCTGCTCTC GGGAGATCAC GCCAAGATCG CGGCCTGGCG GGCCGAACAG TCGCGTCAGC GCACTATTGA GCGGCGCCCA GATCTACTCG GTTTCGATTC GCCCACCGGG GAACATGGTG GCGACGGCCT GAGTTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA