MyabA.00896.a

Putative citrate synthase CitA

CENTER ID: MyabA.00896.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00896.a.A1.GE34909 Full length( MyabA.00896.a ) 1 378
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00896.a.A1.PW35524 Full length( MyabA.00896.a ) 1 378
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_208928
PATRIC ID: fig|36809.5.peg.968
RefSeq: YP_001701678.1
UniProt: B1MJ68
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTVAAPLPAD FAPGLEGVTA FATEIAEPDK DGGSLRYRGV DIEDLVAQRV TFGDVWALLV DGKFGQGLPP AEPFPIPVHT GDVRVDVQAG LAMLAPIWGF EPLLDTEDSV ARSQLARASV MALSYVAQSA RGNSLPAVPQ HIVDECSTVT SRFMTRWQGD PDPKHVEAID AYWVSAAEHG MNASTFTARV IASTGADVAA SLSGAVGAMS GPLHGGAPAR VLPMIEQAEK TGDARGVIRG ILDRREKLMG FGHRVYRAED PRARVLRSTA KRLGAARYEV AAALEQAALA ELRERRPDRV IETNVEFWAA VILDFAQVPT RMMPAMFTCA RTAGWSAHIM EQKRLGKLVR PAALYVGPGP RPIESVEGFG LLHSRVGA
NT Sequence
ATGACGGTTG CAGCGCCGCT ACCAGCGGAT TTCGCACCTG GACTGGAGGG CGTAACCGCC TTCGCCACCG AGATCGCCGA GCCGGACAAG GACGGTGGTT CACTGCGCTA TCGCGGTGTC GACATCGAGG ATCTGGTCGC CCAGCGGGTG ACCTTCGGTG ATGTATGGGC ACTGTTGGTC GACGGCAAGT TCGGACAGGG GCTCCCACCG GCCGAACCTT TCCCCATCCC CGTGCACACC GGCGACGTCC GCGTGGACGT ACAGGCCGGG CTGGCCATGC TGGCACCTAT CTGGGGTTTC GAGCCGCTGC TGGACACCGA GGATTCGGTG GCGCGCAGTC AGCTCGCGCG CGCCTCGGTC ATGGCGCTCT CGTACGTCGC GCAGTCGGCG CGAGGCAATT CCCTGCCCGC CGTTCCGCAA CACATTGTCG ACGAATGTTC AACCGTCACA TCGCGTTTCA TGACCCGCTG GCAGGGTGAT CCCGATCCCA AGCATGTCGA GGCCATTGAC GCGTACTGGG TATCGGCCGC CGAGCACGGC ATGAACGCCT CGACGTTCAC TGCCCGCGTG ATCGCCTCCA CCGGCGCAGA TGTGGCCGCG TCGCTCTCCG GCGCGGTCGG AGCGATGAGC GGGCCATTGC ATGGCGGCGC CCCCGCCCGC GTACTGCCGA TGATCGAGCA GGCCGAGAAG ACCGGCGATG CCCGCGGCGT CATCAGGGGC ATTCTGGACC GCCGCGAAAA GCTCATGGGC TTCGGCCACC GGGTGTATCG CGCCGAAGAC CCGCGCGCGC GGGTGCTGCG CAGCACCGCC AAGCGCCTGG GCGCCGCGCG CTATGAGGTT GCCGCCGCCC TTGAGCAGGC TGCCCTGGCC GAGCTGCGAG AACGCCGCCC CGATCGCGTC ATCGAGACCA ATGTCGAGTT CTGGGCGGCG GTCATTCTCG ACTTCGCGCA GGTGCCGACG CGGATGATGC CCGCCATGTT CACCTGCGCC CGCACCGCGG GCTGGAGTGC GCACATCATG GAGCAGAAAC GTCTCGGCAA GTTGGTGCGC CCGGCCGCGC TGTATGTGGG GCCCGGACCG CGCCCCATCG AATCCGTCGA GGGATTCGGG CTGCTGCACT CTCGCGTCGG TGCG
Details for MyabA.00896.a.A1.GE34909
HARVESTED ON: 4/17/2012
SEQUENCED ON: 4/17/2012
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTVAAPLPA DFAPGLEGVT AFATEIAEPD KDGGSLRYRG VDIEDLVAQR VTFGDVWALL VDGKFGQGLP PAEPFPIPVH TGDVRVDVQA GLAMLAPIWG FEPLLDTEDS VARSQLARAS VMALSYVAQS ARGNSLPAVP QHIVDECSTV TSRFMTRWQG DPDPKHVEAI DAYWVSAAEH GMNASTFTAR VIASTGADVA ASLSGAVGAM SGPLHGGAPA RVLPMIEQAE KTGDARGVIR GILDRREKLM GFGHRVYRAE DPRARVLRST AKRLGAARYE VAAALEQAAL AELRERRPDR VIETNVEFWA AVILDFAQVP TRMMPAMFTC ARTAGWSAHI MEQKRLGKLV RPAALYVGPG PRPIESVEGF GLLHSRV
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacgg ttgcagcgcc gctaccagcg gatttcgcac ctggactgga gggcgtaacc gccttcgcca ccgagatcgc cgagccggac aaggacggtg gttcactgcg ctatcgcggt gtcgacatcg aggatctggt cgcccagcgg gtgaccttcg gtgatgtatg ggcactgttg gtcgacggca agttcggaca ggggctccca ccggccgaac ctttccccat ccccgtgcac accggcgacg tccgcgtgga cgtacaggcc gggctggcca tgctggcacc tatctggggt ttcgagccgc tgctggacac cgaggattcg gtggcgcgca gtcagctcgc gcgcgcctcg gtcatggcgc tctcgtacgt cgcgcagtcg gcgcgaggca attccctgcc cgccgttccg caacacattg tcgacgaatg ttcaaccgtc acatcgcgtt tcatgacccg ctggcagggt gatcccgatc ccaagcatgt cgaggccatt gacgcgtact gggtatcggc cgccgagcac ggcatgaacg cctcgacgtt cactgcccgc gtgatcgcct ccaccggcgc agatgtggcc gcgtcgctct ccggcgcggt cggagcgatg agcgggccat tgcatggcgg cgcccccgcc cgcgtactgc cgatgatcga gcaggccgag aagaccggcg atgcccgcgg cgtcatcagg ggcattctgg accgccgcga aaagctcatg ggcttcggcc accgggtgta tcgcgccgaa gacccgcgcg cgcgggtgct gcgcagcacc gccaagcgcc tgggcgccgc gcgctatgag gttgccgccg cccttgagca ggctgccctg gccgagctgc gagaacgccg ccccgatcgc gtcatcgaga ccaatgtcga gttctgggcg gcggtcattc tcgacttcgc gcaggtgccg acgcggatga tgcccgccat gttcacctgc gcccgcaccg cgggctggag tgcgcacatc atggagcaga aacgtctcgg caagttggtg cgcccggccg cgctgtatgt ggggcccgga ccgcgcccca tcgaatccgt cgagggattc gggctgctgc actctcgcgt cg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTVAAPLPA DFAPGLEGVT AFATEIAEPD KDGGSLRYRG VDIEDLVAQR VTFGDVWALL VDGKFGQGLP PAEPFPIPVH TGDVRVDVQA GLAMLAPIWG FEPLLDTEDS VARSQLARAS VMALSYVAQS ARGNSLPAVP QHIVDECSTV TSRFMTRWQG DPDPKHVEAI DAYWVSAAEH GMNASTFTAR VIASTGADVA ASLSGAVGAM SGPLHGGAPA RVLPMIEQAE KTGDARGVIR GILDRREKLM GFGHRVYRAE DPRARVLRST AKRLGAARYE VAAALEQAAL AELRERRPDR VIETNVEFWA AVILDFAQVP TRMMPAMFTC ARTAGWSAHI MEQKRLGKLV RPAALYVGPG PRPIESVEGF GLLHSRVGA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacggttgca gcgccgctac cagcggattt cgcacctgga ctggagggcg taaccgcctt cgccaccgag atcgccgagc cggacaagga cggtggttca ctgcgctatc gcggtgtcga catcgaggat ctggtcgccc agcgggtgac cttcggtgat gtatgggcac tgttggtcga cggcaagttc ggacaggggc tcccaccggc cgaacctttc cccatccccg tgcacaccgg cgacgtccgc gtggacgtac aggccgggct ggccatgctg gcacctatct ggggtttcga gccgctgctg gacaccgagg attcggtggc gcgcagtcag ctcgcgcgcg cctcggtcat ggcgctctcg tacgtcgcgc agtcggcgcg aggcaattcc ctgcccgccg ttccgcaaca cattgtcgac gaatgttcaa ccgtcacatc gcgtttcatg acccgctggc agggtgatcc cgatcccaag catgtcgagg ccattgacgc gtactgggta tcggccgccg agcacggcat gaacgcctcg acgttcactg cccgcgtgat cgcctccacc ggcgcagatg tggccgcgtc gctctccggc gcggtcggag cgatgagcgg gccattgcat ggcggcgccc ccgcccgcgt actgccgatg atcgagcagg ccgagaagac cggcgatgcc cgcggcgtca tcaggggcat tctggaccgc cgcgaaaagc tcatgggctt cggccaccgg gtgtatcgcg ccgaagaccc gcgcgcgcgg gtgctgcgca gcaccgccaa gcgcctgggc gccgcgcgct atgaggttgc cgccgccctt gagcaggctg ccctggccga gctgcgagaa cgccgccccg atcgcgtcat cgagaccaat gtcgagttct gggcggcggt cattctcgac ttcgcgcagg tgccgacgcg gatgatgccc gccatgttca cctgcgcccg caccgcgggc tggagtgcgc acatcatgga gcagaaacgt ctcggcaagt tggtgcgccc ggccgcgctg tatgtggggc ccggaccgcg ccccatcgaa tccgtcgagg gattcgggct gctgcactct cgcgtcggtg cgtaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for MyabA.00896.a.A1.PW35524
PURIFICATION DATe: 6/8/2012
CONCENTRATION: 33.6mg/ml
OBSERVED MW: 43kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTVAAPLPA DFAPGLEGVT AFATEIAEPD KDGGSLRYRG VDIEDLVAQR VTFGDVWALL VDGKFGQGLP PAEPFPIPVH TGDVRVDVQA GLAMLAPIWG FEPLLDTEDS VARSQLARAS VMALSYVAQS ARGNSLPAVP QHIVDECSTV TSRFMTRWQG DPDPKHVEAI DAYWVSAAEH GMNASTFTAR VIASTGADVA ASLSGAVGAM SGPLHGGAPA RVLPMIEQAE KTGDARGVIR GILDRREKLM GFGHRVYRAE DPRARVLRST AKRLGAARYE VAAALEQAAL AELRERRPDR VIETNVEFWA AVILDFAQVP TRMMPAMFTC ARTAGWSAHI MEQKRLGKLV RPAALYVGPG PRPIESVEGF GLLHSRV
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacgg ttgcagcgcc gctaccagcg gatttcgcac ctggactgga gggcgtaacc gccttcgcca ccgagatcgc cgagccggac aaggacggtg gttcactgcg ctatcgcggt gtcgacatcg aggatctggt cgcccagcgg gtgaccttcg gtgatgtatg ggcactgttg gtcgacggca agttcggaca ggggctccca ccggccgaac ctttccccat ccccgtgcac accggcgacg tccgcgtgga cgtacaggcc gggctggcca tgctggcacc tatctggggt ttcgagccgc tgctggacac cgaggattcg gtggcgcgca gtcagctcgc gcgcgcctcg gtcatggcgc tctcgtacgt cgcgcagtcg gcgcgaggca attccctgcc cgccgttccg caacacattg tcgacgaatg ttcaaccgtc acatcgcgtt tcatgacccg ctggcagggt gatcccgatc ccaagcatgt cgaggccatt gacgcgtact gggtatcggc cgccgagcac ggcatgaacg cctcgacgtt cactgcccgc gtgatcgcct ccaccggcgc agatgtggcc gcgtcgctct ccggcgcggt cggagcgatg agcgggccat tgcatggcgg cgcccccgcc cgcgtactgc cgatgatcga gcaggccgag aagaccggcg atgcccgcgg cgtcatcagg ggcattctgg accgccgcga aaagctcatg ggcttcggcc accgggtgta tcgcgccgaa gacccgcgcg cgcgggtgct gcgcagcacc gccaagcgcc tgggcgccgc gcgctatgag gttgccgccg cccttgagca ggctgccctg gccgagctgc gagaacgccg ccccgatcgc gtcatcgaga ccaatgtcga gttctgggcg gcggtcattc tcgacttcgc gcaggtgccg acgcggatga tgcccgccat gttcacctgc gcccgcaccg cgggctggag tgcgcacatc atggagcaga aacgtctcgg caagttggtg cgcccggccg cgctgtatgt ggggcccgga ccgcgcccca tcgaatccgt cgagggattc gggctgctgc actctcgcgt cg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTVAAPLPA DFAPGLEGVT AFATEIAEPD KDGGSLRYRG VDIEDLVAQR VTFGDVWALL VDGKFGQGLP PAEPFPIPVH TGDVRVDVQA GLAMLAPIWG FEPLLDTEDS VARSQLARAS VMALSYVAQS ARGNSLPAVP QHIVDECSTV TSRFMTRWQG DPDPKHVEAI DAYWVSAAEH GMNASTFTAR VIASTGADVA ASLSGAVGAM SGPLHGGAPA RVLPMIEQAE KTGDARGVIR GILDRREKLM GFGHRVYRAE DPRARVLRST AKRLGAARYE VAAALEQAAL AELRERRPDR VIETNVEFWA AVILDFAQVP TRMMPAMFTC ARTAGWSAHI MEQKRLGKLV RPAALYVGPG PRPIESVEGF GLLHSRVGA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACGGTTGCA GCGCCGCTAC CAGCGGATTT CGCACCTGGA CTGGAGGGCG TAACCGCCTT CGCCACCGAG ATCGCCGAGC CGGACAAGGA CGGTGGTTCA CTGCGCTATC GCGGTGTCGA CATCGAGGAT CTGGTCGCCC AGCGGGTGAC CTTCGGTGAT GTATGGGCAC TGTTGGTCGA CGGCAAGTTC GGACAGGGGC TCCCACCGGC CGAACCTTTC CCCATCCCCG TGCACACCGG CGACGTCCGC GTGGACGTAC AGGCCGGGCT GGCCATGCTG GCACCTATCT GGGGTTTCGA GCCGCTGCTG GACACCGAGG ATTCGGTGGC GCGCAGTCAG CTCGCGCGCG CCTCGGTCAT GGCGCTCTCG TACGTCGCGC AGTCGGCGCG AGGCAATTCC CTGCCCGCCG TTCCGCAACA CATTGTCGAC GAATGTTCAA CCGTCACATC GCGTTTCATG ACCCGCTGGC AGGGTGATCC CGATCCCAAG CATGTCGAGG CCATTGACGC GTACTGGGTA TCGGCCGCCG AGCACGGCAT GAACGCCTCG ACGTTCACTG CCCGCGTGAT CGCCTCCACC GGCGCAGATG TGGCCGCGTC GCTCTCCGGC GCGGTCGGAG CGATGAGCGG GCCATTGCAT GGCGGCGCCC CCGCCCGCGT ACTGCCGATG ATCGAGCAGG CCGAGAAGAC CGGCGATGCC CGCGGCGTCA TCAGGGGCAT TCTGGACCGC CGCGAAAAGC TCATGGGCTT CGGCCACCGG GTGTATCGCG CCGAAGACCC GCGCGCGCGG GTGCTGCGCA GCACCGCCAA GCGCCTGGGC GCCGCGCGCT ATGAGGTTGC CGCCGCCCTT GAGCAGGCTG CCCTGGCCGA GCTGCGAGAA CGCCGCCCCG ATCGCGTCAT CGAGACCAAT GTCGAGTTCT GGGCGGCGGT CATTCTCGAC TTCGCGCAGG TGCCGACGCG GATGATGCCC GCCATGTTCA CCTGCGCCCG CACCGCGGGC TGGAGTGCGC ACATCATGGA GCAGAAACGT CTCGGCAAGT TGGTGCGCCC GGCCGCGCTG TATGTGGGGC CCGGACCGCG CCCCATCGAA TCCGTCGAGG GATTCGGGCT GCTGCACTCT CGCGTCGGTG CGTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA