MyabA.00814.a

Probable catechol-o-methyltransferase

CENTER ID: MyabA.00814.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00814.a.A1.GE30349 Full length( MyabA.00814.a ) 1 253
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00814.a.A1.PS00953 Full length( MyabA.00814.a ) 1 253

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.1355
RefSeq: YP_001702060.1
UniProt: B1MLH9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSLTRTLKQK LPFLRYSFLR AVFGGLNVMR TGQMGDGREE ATAEHVVATA PAGDIDAAIE AIDNFAYDQS ILMNVGDEKG QLLDAAVKRA NPKIALELGA YCGYSGLRIA RAAPGAKFFS IEKSGANASV ARRVWAHAGL ADRATCLTGT ADDGVTLDTL AKDYGFTEGC LDFVFIDHWK DIYLDDLQRL LERGWLHPGT IVVADNVRFP GAPKYRAYMK DQQGKLWQTI EHETHVEYQT LVKDLVLESE YLG
NT Sequence
GTGAGTCTTA CGAGAACCCT CAAGCAGAAA CTCCCATTTC TGCGGTATTC GTTTCTGCGG GCGGTGTTCG GCGGCCTCAA CGTCATGCGC ACCGGTCAGA TGGGCGATGG TCGCGAGGAA GCCACCGCCG AGCATGTCGT CGCGACCGCC CCGGCCGGGG ATATCGACGC CGCCATCGAG GCGATCGACA ACTTCGCGTA CGACCAGTCG ATTCTCATGA ACGTCGGCGA CGAAAAGGGC CAGCTGCTCG ATGCCGCCGT CAAACGCGCC AACCCCAAGA TCGCCCTGGA GCTGGGCGCC TATTGCGGCT ACAGCGGCCT ACGGATCGCG CGGGCGGCCC CGGGCGCCAA ATTCTTCTCC ATCGAGAAAT CAGGCGCCAA CGCCTCCGTG GCACGCCGAG TCTGGGCACA CGCCGGTCTG GCGGATCGGG CCACCTGTCT CACCGGGACG GCCGATGACG GCGTCACCTT GGACACCTTG GCCAAGGACT ACGGGTTTAC CGAGGGATGT CTGGATTTCG TGTTCATCGA CCATTGGAAG GACATTTATC TCGATGACCT GCAGCGGCTT CTGGAACGGG GCTGGCTGCA TCCGGGCACG ATCGTCGTCG CCGATAACGT CCGCTTCCCC GGCGCTCCGA AGTACCGCGC GTACATGAAG GACCAGCAGG GCAAGCTCTG GCAGACCATC GAGCACGAGA CCCACGTGGA GTATCAGACT CTGGTGAAGG ATCTGGTGCT GGAGTCGGAG TACCTGGGA
Details for MyabA.00814.a.A1.GE30349
HARVESTED ON: 9/8/2010
SEQUENCED ON: 9/17/2010
EXPECTED MW: 30kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSLTRTLK QKLPFLRYSF LRAVFGGLNV MRTGQMGDGR EEATAEHVVA TAPAGDIDAA IEAIDNFAYD QSILMNVGDE KGQLLDAAVK RANPKIALEL GAYCGYSGLR IARAAPGAKF FSIEKSGANA SVARRVWAHA GLADRATCLT GTADDGVTLD TLAKDYGFTE GCLDFVFIDH WKDIYLDDLQ RLLERGWLHP GTIVVADNVR FPGAPKYRAY MKDQQGKLWQ TIEHETHVEY QTLVKDLVLE SE
Validated NT Sequence
actccgactc cagcaccaga tccttcacca gagtctgata ctccacgtgg gtctcgtgct cgatggtctg ccagagcttg ccctgctggt ccttcatgta cgcgcggtac ttcggagcgc cggggaagcg gacgttatcg gcgacgacga tcgtgcccgg atgcagccag ccccgttcca gaagccgctg caggtcatcg agataaatgt ccttccaatg gtcgatgaac acgaaatcca gacatccctc ggtaaacccg tagtccttgg ccaaggtgtc caaggtgacg ccgtcatcgg ccgtcccggt gagacaggtg gcccgatccg ccagaccggc gtgtgcccag actcggcgtg ccacggaggc gttggcgcct gatttctcga tggagaagaa tttggcgccc ggggccgccc gcgcgatccg taggccgctg tagccgcaat aggcgcccag ctccagggcg atcttggggt tggcgcgttt gacggcggca tcgagcagct ggcccttttc gtcgccgacg ttcatgagaa tcgactggtc gtacgcgaag ttgtcgatcg cctcgatggc ggcgtcgata tccccggccg gggcggtcgc gacgacatgc tcggcggtgg cttcctcgcg accatcgccc atctgaccgg tgcgcatgac gttgaggccg ccgaacaccg cccgcagaaa cgaataccgc agaaatggga gtttctgctt gagggttctc gtaagactca ccatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSLTRTLK QKLPFLRYSF LRAVFGGLNV MRTGQMGDGR EEATAEHVVA TAPAGDIDAA IEAIDNFAYD QSILMNVGDE KGQLLDAAVK RANPKIALEL GAYCGYSGLR IARAAPGAKF FSIEKSGANA SVARRVWAHA GLADRATCLT GTADDGVTLD TLAKDYGFTE GCLDFVFIDH WKDIYLDDLQ RLLERGWLHP GTIVVADNVR FPGAPKYRAY MKDQQGKLWQ TIEHETHVEY QTLVKDLVLE SEYLG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagtctt acgagaaccc tcaagcagaa actcccattt ctgcggtatt cgtttctgcg ggcggtgttc ggcggcctca acgtcatgcg caccggtcag atgggcgatg gtcgcgagga agccaccgcc gagcatgtcg tcgcgaccgc cccggccggg gatatcgacg ccgccatcga ggcgatcgac aacttcgcgt acgaccagtc gattctcatg aacgtcggcg acgaaaaggg ccagctgctc gatgccgccg tcaaacgcgc caaccccaag atcgccctgg agctgggcgc ctattgcggc tacagcggcc tacggatcgc gcgggcggcc ccgggcgcca aattcttctc catcgagaaa tcaggcgcca acgcctccgt ggcacgccga gtctgggcac acgccggtct ggcggatcgg gccacctgtc tcaccgggac ggccgatgac ggcgtcacct tggacacctt ggccaaggac tacgggttta ccgagggatg tctggatttc gtgttcatcg accattggaa ggacatttat ctcgatgacc tgcagcggct tctggaacgg ggctggctgc atccgggcac gatcgtcgtc gccgataacg tccgcttccc cggcgctccg aagtaccgcg cgtacatgaa ggaccagcag ggcaagctct ggcagaccat cgagcacgag acccacgtgg agtatcagac tctggtgaag gatctggtgc tggagtcgga gtacctggga taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for MyabA.00814.a.A1.PS00953
PURIFICATION DATe: 2/1/2011
CONCENTRATION: 21.32mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSLTRTLK QKLPFLRYSF LRAVFGGLNV MRTGQMGDGR EEATAEHVVA TAPAGDIDAA IEAIDNFAYD QSILMNVGDE KGQLLDAAVK RANPKIALEL GAYCGYSGLR IARAAPGAKF FSIEKSGANA SVARRVWAHA GLADRATCLT GTADDGVTLD TLAKDYGFTE GCLDFVFIDH WKDIYLDDLQ RLLERGWLHP GTIVVADNVR FPGAPKYRAY MKDQQGKLWQ TIEHETHVEY QTLVKDLVLE SE
Validated NT Sequence
actccgactc cagcaccaga tccttcacca gagtctgata ctccacgtgg gtctcgtgct cgatggtctg ccagagcttg ccctgctggt ccttcatgta cgcgcggtac ttcggagcgc cggggaagcg gacgttatcg gcgacgacga tcgtgcccgg atgcagccag ccccgttcca gaagccgctg caggtcatcg agataaatgt ccttccaatg gtcgatgaac acgaaatcca gacatccctc ggtaaacccg tagtccttgg ccaaggtgtc caaggtgacg ccgtcatcgg ccgtcccggt gagacaggtg gcccgatccg ccagaccggc gtgtgcccag actcggcgtg ccacggaggc gttggcgcct gatttctcga tggagaagaa tttggcgccc ggggccgccc gcgcgatccg taggccgctg tagccgcaat aggcgcccag ctccagggcg atcttggggt tggcgcgttt gacggcggca tcgagcagct ggcccttttc gtcgccgacg ttcatgagaa tcgactggtc gtacgcgaag ttgtcgatcg cctcgatggc ggcgtcgata tccccggccg gggcggtcgc gacgacatgc tcggcggtgg cttcctcgcg accatcgccc atctgaccgg tgcgcatgac gttgaggccg ccgaacaccg cccgcagaaa cgaataccgc agaaatggga gtttctgctt gagggttctc gtaagactca ccatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSLTRTLK QKLPFLRYSF LRAVFGGLNV MRTGQMGDGR EEATAEHVVA TAPAGDIDAA IEAIDNFAYD QSILMNVGDE KGQLLDAAVK RANPKIALEL GAYCGYSGLR IARAAPGAKF FSIEKSGANA SVARRVWAHA GLADRATCLT GTADDGVTLD TLAKDYGFTE GCLDFVFIDH WKDIYLDDLQ RLLERGWLHP GTIVVADNVR FPGAPKYRAY MKDQQGKLWQ TIEHETHVEY QTLVKDLVLE SEYLG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAGTCTT ACGAGAACCC TCAAGCAGAA ACTCCCATTT CTGCGGTATT CGTTTCTGCG GGCGGTGTTC GGCGGCCTCA ACGTCATGCG CACCGGTCAG ATGGGCGATG GTCGCGAGGA AGCCACCGCC GAGCATGTCG TCGCGACCGC CCCGGCCGGG GATATCGACG CCGCCATCGA GGCGATCGAC AACTTCGCGT ACGACCAGTC GATTCTCATG AACGTCGGCG ACGAAAAGGG CCAGCTGCTC GATGCCGCCG TCAAACGCGC CAACCCCAAG ATCGCCCTGG AGCTGGGCGC CTATTGCGGC TACAGCGGCC TACGGATCGC GCGGGCGGCC CCGGGCGCCA AATTCTTCTC CATCGAGAAA TCAGGCGCCA ACGCCTCCGT GGCACGCCGA GTCTGGGCAC ACGCCGGTCT GGCGGATCGG GCCACCTGTC TCACCGGGAC GGCCGATGAC GGCGTCACCT TGGACACCTT GGCCAAGGAC TACGGGTTTA CCGAGGGATG TCTGGATTTC GTGTTCATCG ACCATTGGAA GGACATTTAT CTCGATGACC TGCAGCGGCT TCTGGAACGG GGCTGGCTGC ATCCGGGCAC GATCGTCGTC GCCGATAACG TCCGCTTCCC CGGCGCTCCG AAGTACCGCG CGTACATGAA GGACCAGCAG GGCAAGCTCT GGCAGACCAT CGAGCACGAG ACCCACGTGG AGTATCAGAC TCTGGTGAAG GATCTGGTGC TGGAGTCGGA GTACCTGGGA TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA