MyabA.00781.a

Putative acetyl-CoA C-acyltransferase (Thiolase)

CENTER ID: MyabA.00781.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00781.a.A1.GE30345 Full length( MyabA.00781.a ) 1 426
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.1036
RefSeq: YP_001701746.1
UniProt: B1MJD6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTGSAKRRAV IVAGARTPFV RAFGEFTRMD TIALADEAVR GLLDNTKVSS SEIEAIVWGG VILPAGAPNV AREIALDLKL GHGVEGHTVT RACASGLQAI TTAAAAIERG EYDVMIAGGS DSTSNAGVNL PQKLIHAAAP LALGKPKPKD YLDAAWSLAP FTGLLPRRPK IEERTTGEVM GESADKMAKI HGITRQAQDE FAVRSHRRAA AAIASGRFDR EVVPVAGVTR DGLVRENTSV EKLATLKPVF NRDGTVTAGN ASPLTDGAAA VLLMSEEKAR ALGLRPLAAF RSWSYVSVDP ADEVLIGPAI SMPRALEKAG LDLDDVDYID IHEAFAAQTL SVLEALGSKK WATDRLGRSE AVGTPDIDKV NVHGGSVSIG HPFGATGARM VTTMANELAL SGKGTALLGI CAAGGHGASA VLERVD
NT Sequence
ATGACCGGAT CTGCCAAGCG CCGCGCTGTC ATTGTCGCGG GCGCACGCAC CCCATTTGTT CGTGCCTTCG GTGAGTTCAC CCGCATGGAC ACCATTGCGC TGGCCGACGA GGCGGTGCGC GGCTTGCTGG ACAACACCAA GGTCTCTTCC AGTGAGATCG AGGCGATCGT CTGGGGCGGG GTGATCCTGC CCGCGGGCGC GCCGAACGTC GCCCGCGAGA TCGCACTCGA TCTCAAGCTT GGGCACGGCG TCGAAGGCCA CACCGTCACG CGCGCGTGCG CTTCCGGTCT GCAAGCCATC ACCACCGCGG CCGCCGCGAT CGAGCGCGGC GAATACGACG TGATGATCGC CGGTGGCTCC GACTCCACCT CGAACGCGGG AGTCAACCTG CCGCAGAAGC TGATTCACGC GGCCGCCCCG TTGGCCCTGG GTAAGCCCAA GCCGAAGGAT TACCTCGACG CCGCATGGAG CCTGGCCCCG TTCACCGGAC TGCTGCCACG CCGGCCCAAG ATCGAGGAGC GCACCACCGG TGAGGTGATG GGGGAGTCGG CCGACAAAAT GGCCAAGATT CACGGCATCA CCCGCCAGGC CCAGGACGAG TTCGCGGTCC GCTCGCACCG CCGTGCGGCC GCGGCCATCG CGTCGGGACG ATTCGACCGG GAGGTCGTTC CCGTCGCGGG CGTGACCCGC GACGGCCTGG TCCGTGAGAA CACCAGCGTG GAAAAGCTGG CCACGCTCAA GCCCGTCTTC AATCGCGACG GCACCGTGAC CGCGGGTAAC GCCAGTCCGC TCACCGATGG TGCCGCGGCG GTCCTGCTCA TGAGTGAGGA GAAAGCCCGG GCGCTCGGCC TGCGGCCGTT GGCGGCCTTC CGCTCCTGGA GCTACGTCAG CGTGGATCCG GCGGACGAGG TGCTCATCGG ACCGGCGATC TCGATGCCCC GTGCGCTCGA GAAGGCGGGA CTCGATCTCG ATGACGTCGA CTACATCGAC ATTCACGAAG CTTTTGCCGC ACAGACCCTT TCGGTGCTGG AGGCACTGGG CTCCAAGAAG TGGGCCACCG ACCGGTTGGG GCGCTCGGAG GCGGTCGGCA CTCCCGATAT CGACAAGGTC AACGTGCACG GCGGTTCGGT GTCGATCGGA CATCCGTTCG GCGCCACCGG CGCCCGCATG GTCACCACAA TGGCCAACGA GCTGGCGCTC AGCGGGAAAG GCACGGCACT GCTGGGTATT TGCGCAGCAG GCGGTCACGG TGCCAGTGCG GTACTGGAAC GGGTGGAC
Details for MyabA.00781.a.A1.GE30345
HARVESTED ON: 9/8/2010
SEQUENCED ON: 9/17/2010
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 78
PERCENT COVERAGE: 85
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTGSAKRRA VIVAGARTPF VRAFGEFTRX DTIXXADEAV RGLLDNTKVS SSEIEAIVWG GVILPAGAPN VAREIALDLK LGHGVEGHTV TRACASGLQA ITTAAAAIER GEYDVMIAGG SDSTSNAGVN LPQKLIHAAA PLALGKPKPK DYLDAAWSLA PFTGLLPRRP KIEERTTGEV MGESADKMAK IHGITRQAQD EFAVRSHRRA AAAIASGRFX XXVVPVAGVT RDGLVRENTS VEKLXTLKPV FXRDXTVTAG NAXPLTDGAA AXLLMXXXKA RALXLXPLAA XXXXXLRQRG SGXXXAHXXX XXRCPXXXXX XGXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX
Validated NT Sequence
ttgtttactt taagaaggag atataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atgaccggat ctgccaagcg ccgcgctgtc attgtcgcgg gcgcacgcac cccatttgtt cgtgccttcg gtgagttcac ccgcntggac accattgnnn nngccgacga ggcggtgcgc ggcttgctgg acaacaccaa ggtctcttcc agtgagatcg aggcgatcgt ctggggcggg gtgatcctgc ccgcgggcgc gccgaacgtc gcccgcgaga tcgcactcga tctcaagctt gggcacggcg tcgaaggcca caccgtcacg cgcgcgtgcg cttccggtct gcaagccatc accaccgcgg ccgccgcgat cgagcgcggc gaatacgacg tgatgatcgc cggtggctcc gactccacct cgaacgcggg agtcaacctg ccgcagaagc tgattcacgc ggccgccccg ttggccctgg gtaagcccaa gccgaaggat tacctcgacg ccgcatggag cctggccccg ttcaccggac tgctgccacg ccggcccaag atcgaggagc gcaccaccgg tgaggtgatg ggggagtcgg ccgacaaaat ggccaagatt cacggcatca cccgccaggc ccaggacgag ttcgcggtcc gctcgcaccg ccgtgcggcc gcggccatcg cgtcnggacg attcnnnnng gangtcgttc ccgtcgcggg cgtgacccgc gacggcctgg tccgtgagaa caccagcgtg gaaaagctgg ncacgctcaa gcccgtcttc natcgcgacn gcaccgtgac cgcgggtaac gccngtccgc tcacngatgg tgccgcggcg gnnctgctca tgantganna gaaagcccgg gcgctcnnnc tgcngccgtt ggcggccnnc cnnnncnggg nnctacgtca gcgtggatcc ggnnnnnnan nngctcatnc nnnncngnnn nctcgntgcc ccnngngnnc nnnnanngnn ggnnnnnatn nnnannnnnn cnacttnnnn nntnnnnnan ctttnncnnn nanannnnnt tnnnnnnntn gncnnngnnn ncnnnnnann nngnnnnnnn nnnnnnnnng nngnnnnnnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTGSAKRRA VIVAGARTPF VRAFGEFTRM DTIALADEAV RGLLDNTKVS SSEIEAIVWG GVILPAGAPN VAREIALDLK LGHGVEGHTV TRACASGLQA ITTAAAAIER GEYDVMIAGG SDSTSNAGVN LPQKLIHAAA PLALGKPKPK DYLDAAWSLA PFTGLLPRRP KIEERTTGEV MGESADKMAK IHGITRQAQD EFAVRSHRRA AAAIASGRFD REVVPVAGVT RDGLVRENTS VEKLATLKPV FNRDGTVTAG NASPLTDGAA AVLLMSEEKA RALGLRPLAA FRSWSYVSVD PADEVLIGPA ISMPRALEKA GLDLDDVDYI DIHEAFAAQT LSVLEALGSK KWATDRLGRS EAVGTPDIDK VNVHGGSVSI GHPFGATGAR MVTTMANELA LSGKGTALLG ICAAGGHGAS AVLERVD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccggatct gccaagcgcc gcgctgtcat tgtcgcgggc gcacgcaccc catttgttcg tgccttcggt gagttcaccc gcatggacac cattgcgctg gccgacgagg cggtgcgcgg cttgctggac aacaccaagg tctcttccag tgagatcgag gcgatcgtct ggggcggggt gatcctgccc gcgggcgcgc cgaacgtcgc ccgcgagatc gcactcgatc tcaagcttgg gcacggcgtc gaaggccaca ccgtcacgcg cgcgtgcgct tccggtctgc aagccatcac caccgcggcc gccgcgatcg agcgcggcga atacgacgtg atgatcgccg gtggctccga ctccacctcg aacgcgggag tcaacctgcc gcagaagctg attcacgcgg ccgccccgtt ggccctgggt aagcccaagc cgaaggatta cctcgacgcc gcatggagcc tggccccgtt caccggactg ctgccacgcc ggcccaagat cgaggagcgc accaccggtg aggtgatggg ggagtcggcc gacaaaatgg ccaagattca cggcatcacc cgccaggccc aggacgagtt cgcggtccgc tcgcaccgcc gtgcggccgc ggccatcgcg tcgggacgat tcgaccggga ggtcgttccc gtcgcgggcg tgacccgcga cggcctggtc cgtgagaaca ccagcgtgga aaagctggcc acgctcaagc ccgtcttcaa tcgcgacggc accgtgaccg cgggtaacgc cagtccgctc accgatggtg ccgcggcggt cctgctcatg agtgaggaga aagcccgggc gctcggcctg cggccgttgg cggccttccg ctcctggagc tacgtcagcg tggatccggc ggacgaggtg ctcatcggac cggcgatctc gatgccccgt gcgctcgaga aggcgggact cgatctcgat gacgtcgact acatcgacat tcacgaagct tttgccgcac agaccctttc ggtgctggag gcactgggct ccaagaagtg ggccaccgac cggttggggc gctcggaggc ggtcggcact cccgatatcg acaaggtcaa cgtgcacggc ggttcggtgt cgatcggaca tccgttcggc gccaccggcg cccgcatggt caccacaatg gccaacgagc tggcgctcag cgggaaaggc acggcactgc tgggtatttg cgcagcaggc ggtcacggtg ccagtgcggt actggaacgg gtggactaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa