SSGCID: Target Page

MyabA.00762.a

Short-chain dehydrogenase/reductase

CENTER ID:	MyabA.00762.a
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.00762.a.A1.GE30342	Full length( MyabA.00762.a )		1	262

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|36809.5.peg.638
RefSeq:	YP_001701350.1
UniProt:	B1MH12

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNLAEAPTEI EGHGLLKGKA VVVTAAAGTG IGSSTARRAL VEGADVVVSD YHERRLTETR DELAALGLGK VAAVVCDVTS TEAVDALITE SVAALGRIDV LVNNAGLGGE TPVADMTDDQ WDRVLDVTLT STFRATRAAL RYFREAGHGG VIVNNASVLG WRAQYGQSHY AAAKAGVMAL TRCSAIEAVE HGVRVNAVAP SIARHKFLDK VTSSDLLDKL SSDEAFGRAA EPWEVAATIA FLASDYSSYL TGEVISVSSQ RA

NT Sequence

ATGAACCTGG CAGAGGCGCC GACAGAGATC GAGGGCCATG GCCTCCTGAA GGGCAAGGCC GTCGTCGTGA CCGCAGCGGC CGGCACCGGA ATCGGCTCGT CGACCGCGCG GCGGGCACTG GTCGAGGGTG CCGACGTGGT CGTCTCCGAC TATCACGAGC GTCGCCTGAC CGAGACGCGG GACGAACTCG CCGCGCTAGG GCTCGGCAAG GTCGCGGCGG TGGTGTGCGA TGTCACCTCT ACCGAGGCGG TCGACGCATT GATCACAGAA TCCGTTGCGG CCCTGGGCCG TATCGATGTG CTGGTGAACA ATGCGGGCCT TGGTGGAGAG ACTCCGGTCG CCGATATGAC CGACGACCAG TGGGACAGGG TGCTCGATGT GACCCTCACC TCGACTTTCC GGGCGACCCG TGCGGCCCTG CGCTATTTCC GCGAGGCGGG CCACGGCGGG GTGATCGTCA ACAACGCGAG CGTGTTGGGC TGGCGCGCGC AATACGGACA GTCGCACTAT GCCGCCGCCA AGGCCGGCGT CATGGCCCTG ACTCGCTGCA GTGCCATCGA GGCCGTCGAG CACGGGGTGC GCGTCAACGC GGTCGCTCCG AGCATCGCTC GGCACAAGTT CCTCGACAAG GTGACTTCCT CGGACCTGCT GGACAAACTG TCCTCCGACG AGGCCTTCGG CCGCGCTGCC GAGCCGTGGG AGGTCGCGGC CACCATCGCC TTCCTGGCCA GCGACTATTC GAGCTACCTG ACCGGCGAGG TCATCTCGGT ATCCAGCCAG CGGGCG

Details for MyabA.00762.a.A1.GE30342

HARVESTED ON:	9/8/2010
SEQUENCED ON:	9/17/2010
EXPECTED MW:	29kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	97
PERCENT COVERAGE:	106

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMNLAEAPTE IEGHGLLKGK AVVVTAAAGT GIGSSTARRA LVEGADVVVS DYHERRLTET RDELAALGLG KVAAVVCDVT STEAVDALIT ESVAALGRID VLVNNAGLGG ETPVADMTDD QWDRVLDVTL TSTFRATRAA LRYFREAGHG GVIVNNASVL GWRAQYGQSH YAAAKAGVMA LTRCSAIEAV EHGVRVNAVA PSIARHKFLD KVTSSDLLDK LSSDEAFGRA AEPWXVAATI AFLXSDYSSY LTXXVISVSS PAGVIXSTXK FCXXASXGXX C

Validated NT Sequence

tttgtnnnnt ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgaacctg gcagaggcgc cgacagagat cgagggccat ggcctcctga agggcaaggc cgtcgtcgtg accgcagcgg ccggcaccgg aatcggctcg tcgaccgcgc ggcgggcact ggtcgagggt gccgacgtgg tcgtctccga ctatcacgag cgtcgcctga ccgagacgcg ggacgaactc gccgcgctag ggctcggcaa ggtcgcggcg gtggtgtgcg atgtcacctc taccgaggcg gtcgacgcat tgatcacaga atccgttgcg gccctgggcc gtatcgatgt gctggtgaac aatgcgggcc ttggtggaga gactccggtc gccgatatga ccgacgacca gtgggacagg gtgctcgatg tgaccctcac ctcgactttc cgggcgaccc gtgcggccct gcgctatttc cgcgaggcgg gccacggcgg ggtgatcgtc aacaacgcga gcgtgttggg ctggcgcgcg caatacggac agtcgcacta tgccgccgcc aaggccggcg tcatggccct gactcgctgc agtgccatcg aggccgtcga gcacggggtg cgcgtcaacg cggtcgctcc gagcatcgct cggcacaagt tcctcgacaa ggtgacttcc tcggacctgc tggacaaact gtcctccgac gaggccttcg gncgcgctgc cgagccgtgg gangtcgcgg ccaccatcgc cttcctggnc agcgactatt cnagctacct gaccngcgan gtcatctcng tntccagccc ngcgggcgta ataaanagca cnnnnaagtt ctgcagncna gcttctcnag ganccnnntg ctaacaaann cnnnaangnn ngctnanttg gntgctgccn ccgctgnann nnnanna

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaacctggca gaggcgccga cagagatcga gggccatggc ctcctgaagg gcaaggccgt cgtcgtgacc gcagcggccg gcaccggaat cggctcgtcg accgcgcggc gggcactggt cgagggtgcc gacgtggtcg tctccgacta tcacgagcgt cgcctgaccg agacgcggga cgaactcgcc gcgctagggc tcggcaaggt cgcggcggtg gtgtgcgatg tcacctctac cgaggcggtc gacgcattga tcacagaatc cgttgcggcc ctgggccgta tcgatgtgct ggtgaacaat gcgggccttg gtggagagac tccggtcgcc gatatgaccg acgaccagtg ggacagggtg ctcgatgtga ccctcacctc gactttccgg gcgacccgtg cggccctgcg ctatttccgc gaggcgggcc acggcggggt gatcgtcaac aacgcgagcg tgttgggctg gcgcgcgcaa tacggacagt cgcactatgc cgccgccaag gccggcgtca tggccctgac tcgctgcagt gccatcgagg ccgtcgagca cggggtgcgc gtcaacgcgg tcgctccgag catcgctcgg cacaagttcc tcgacaaggt gacttcctcg gacctgctgg acaaactgtc ctccgacgag gccttcggcc gcgctgccga gccgtgggag gtcgcggcca ccatcgcctt cctggccagc gactattcga gctacctgac cggcgaggtc atctcggtat ccagccagcg ggcgtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa