MyabA.00681.a

Probable NAD(P) transhydrogenase, alpha1 subunit PntAA

CENTER ID: MyabA.00681.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00681.a.A1.GE30332 Full length( MyabA.00681.a ) 1 362
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.4653
RefSeq: YP_001705302.1
UniProt: B1ML03

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDQPVTIGV VRESGEDERR VALVPKAIAG LIGKGVNIVI ESGAGERALL PDELYTEAGA TVGDAWSADV VVKVAPPTAA EVARLRQGQT LIGFLAPRNA ENSIGALRSA GVQAFAVEAI PRISRAQVMD ALSSQANVAG YKSVLVAASE STRFFPMLTT AAGTVKPALV LVLGVGVAGL QALATAKRLG ARTTGYDVRP EVADQVRSVG AQWLDLGIDA AGEGGYAREL TDEERQKQQQ ALEDAIKGFD VVITTALVPG RPAPRLVTAA AVQGMKPGSV VVDLAGETGG NCELTEPGQT VVKHGVTIAS PLNLPATMPE HASELYAKNI TSVLELLIKD GALAPDFDDE IVAGSCVTRE VS
NT Sequence
ATGACCGATC AACCAGTCAC TATCGGAGTT GTCCGAGAGT CAGGTGAGGA CGAGCGCCGG GTCGCGCTGG TACCGAAGGC GATCGCCGGC CTGATTGGCA AGGGTGTGAA CATCGTCATA GAAAGTGGTG CAGGAGAGCG CGCCCTGCTG CCGGACGAGC TGTACACCGA AGCGGGGGCG ACCGTGGGCG ATGCGTGGTC CGCGGATGTC GTGGTGAAGG TGGCGCCGCC CACCGCCGCC GAGGTCGCTC GCCTGCGGCA GGGACAGACC CTGATCGGCT TCCTGGCGCC GCGCAATGCC GAGAACAGCA TCGGTGCGCT CAGGAGCGCG GGCGTGCAAG CCTTTGCGGT GGAAGCCATT CCGCGTATCT CGCGGGCCCA GGTGATGGAC GCGTTGTCAT CGCAGGCCAA TGTTGCCGGC TACAAGTCGG TGCTCGTGGC GGCTTCGGAG TCGACCCGTT TCTTCCCGAT GCTGACCACC GCTGCCGGGA CCGTGAAGCC GGCACTGGTG CTGGTTCTCG GCGTCGGCGT CGCGGGCCTG CAGGCCCTGG CCACCGCCAA GCGGCTCGGT GCGCGCACCA CCGGCTACGA CGTGCGGCCC GAGGTGGCCG ACCAGGTCCG GTCGGTGGGT GCCCAGTGGC TCGATCTGGG CATCGACGCG GCCGGGGAGG GCGGGTACGC CCGCGAGCTG ACCGATGAAG AGCGCCAAAA GCAGCAGCAG GCGCTCGAAG ACGCCATCAA GGGATTCGAT GTCGTCATCA CCACCGCACT GGTGCCGGGA CGCCCGGCGC CGCGTCTGGT GACCGCCGCG GCCGTGCAAG GCATGAAGCC GGGATCGGTG GTCGTCGATC TCGCCGGTGA GACCGGCGGC AACTGTGAGC TCACCGAGCC GGGGCAGACC GTCGTCAAGC ACGGCGTCAC CATCGCCTCG CCCCTGAACC TGCCGGCCAC CATGCCCGAG CACGCCAGCG AGCTGTATGC CAAGAACATC ACGTCCGTTC TCGAGCTCCT CATCAAGGAC GGCGCCCTGG CGCCCGACTT CGATGACGAG ATCGTCGCGG GCTCCTGCGT CACCCGGGAG GTCTCC
Details for MyabA.00681.a.A1.GE30332
HARVESTED ON: 9/8/2010
SEQUENCED ON: 9/17/2010
EXPECTED MW: 39kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 80
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTDQPVTIG VVRESGEDER RVALVPKAIA GLIGKGVNIV IESGAGERAL LPDELYTEAG ATVGDAWSAD VVVKVAPPTA AEVARLRQGQ TLIGFLAPRN AENSIGALRS AGVQAFAVEA IPRISRAQVM DALSSQANVA GYKSVLVAAS ESTRFFPMLT TAAGTVKPAL VLVLGVGVAG LQALATAKRL GARTTGYDVR PEVADQVRSV GAQWLDLGID AAGXGGYARE LTDEERXKQQ QALEDAIKGF DVVITTALVP GRPAPRLVTA AXVXGMKPGS VVVXLAG
Validated NT Sequence
tttntttact ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgaccgat caaccagtca ctatcggagt tgtccgagag tcaggtgagg acgagcgccg ggtcgcgctg gtaccgaagg cgatcgccgg cctgattggc aagggtgtga acatcgtcat agaaagtggt gcaggagagc gcgccctgct gccggacgag ctgtacaccg aagcgggggc gaccgtgggc gatgcgtggt ccgcggatgt cgtggtgaag gtggcgccgc ccaccgccgc cgaggtcgct cgcctgcggc agggacagac cctgatcggc ttcctggcgc cgcgcaatgc cgagaacagc atcggtgcgc tcaggagcgc gggcgtgcaa gcctttgcgg tggaagccat tccgcgtatc tcgcgggccc aggtgatgga cgcgttgtca tcgcaggcca atgttgccgg ctacaagtcg gtgctcgtgg cggcttcgga gtcgacccgt ttcttcccga tgctgaccac cgctgccggg accgtgaagc cggcactggt gctggttctc ggcgtcggcg tcgcgggcct gcaggccctg gccaccgcca agcggctcgg tgcgcgcacc accggctacg acgtgcggcc cgaggtggcc gaccaggtcc ggtcggtggg tgcccagtgg ctcgatctgg gcatcgacgc ggccgggnag ggcgggtacg cccgcgagct gaccgatgaa gagcgccnaa agcagcagca ggcgctcgaa gacgccatca agggattcga tgtcgtcatc accaccgcac tggtgccggg acgcccggcg ccncgtctgg tgaccgccgc gnccgtgcna ggnatgaagc cnggntcggt ggtcgtcnnt ctcgccgggt gagancnggc ggcaactgtg agcnnnncna gnngggggca gancgtcgtc aagcanngcg tcncnntnnn ctnannntgn nnnnnnnnnn tnnnnnnanc acnnnnnnnn nnnngnngan catnnnnnnn cnnnctnnnn cnnnnnncnn nnnngnnncc ncnnnannnn annannnnnt cnnnnnnnnn nnnnnnnnnn nnnggnnnnn annnnnnnnn nnnnnnnnnn nannnnnnnn tncnnnnnna nnnnnnnnnn nnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDQPVTIG VVRESGEDER RVALVPKAIA GLIGKGVNIV IESGAGERAL LPDELYTEAG ATVGDAWSAD VVVKVAPPTA AEVARLRQGQ TLIGFLAPRN AENSIGALRS AGVQAFAVEA IPRISRAQVM DALSSQANVA GYKSVLVAAS ESTRFFPMLT TAAGTVKPAL VLVLGVGVAG LQALATAKRL GARTTGYDVR PEVADQVRSV GAQWLDLGID AAGEGGYARE LTDEERQKQQ QALEDAIKGF DVVITTALVP GRPAPRLVTA AAVQGMKPGS VVVDLAGETG GNCELTEPGQ TVVKHGVTIA SPLNLPATMP EHASELYAKN ITSVLELLIK DGALAPDFDD EIVAGSCVTR EVS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgatcaa ccagtcacta tcggagttgt ccgagagtca ggtgaggacg agcgccgggt cgcgctggta ccgaaggcga tcgccggcct gattggcaag ggtgtgaaca tcgtcataga aagtggtgca ggagagcgcg ccctgctgcc ggacgagctg tacaccgaag cgggggcgac cgtgggcgat gcgtggtccg cggatgtcgt ggtgaaggtg gcgccgccca ccgccgccga ggtcgctcgc ctgcggcagg gacagaccct gatcggcttc ctggcgccgc gcaatgccga gaacagcatc ggtgcgctca ggagcgcggg cgtgcaagcc tttgcggtgg aagccattcc gcgtatctcg cgggcccagg tgatggacgc gttgtcatcg caggccaatg ttgccggcta caagtcggtg ctcgtggcgg cttcggagtc gacccgtttc ttcccgatgc tgaccaccgc tgccgggacc gtgaagccgg cactggtgct ggttctcggc gtcggcgtcg cgggcctgca ggccctggcc accgccaagc ggctcggtgc gcgcaccacc ggctacgacg tgcggcccga ggtggccgac caggtccggt cggtgggtgc ccagtggctc gatctgggca tcgacgcggc cggggagggc gggtacgccc gcgagctgac cgatgaagag cgccaaaagc agcagcaggc gctcgaagac gccatcaagg gattcgatgt cgtcatcacc accgcactgg tgccgggacg cccggcgccg cgtctggtga ccgccgcggc cgtgcaaggc atgaagccgg gatcggtggt cgtcgatctc gccggtgaga ccggcggcaa ctgtgagctc accgagccgg ggcagaccgt cgtcaagcac ggcgtcacca tcgcctcgcc cctgaacctg ccggccacca tgcccgagca cgccagcgag ctgtatgcca agaacatcac gtccgttctc gagctcctca tcaaggacgg cgccctggcg cccgacttcg atgacgagat cgtcgcgggc tcctgcgtca cccgggaggt ctcctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa