MyabA.00629.d

Bifunctional enzyme MbtA : salicyl-AMP ligase + salicyl-S-ArCP synthetase

CENTER ID: MyabA.00629.d
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00629.d.B2.GE40015 ordered domain 20 550
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.2288
UniProt: B1MAQ7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTQPRSTTTA SSAATPALDG FVPFPSVRAD EYRRAGYWTG NTVESLLRVS AARRPCHPAV IDEHRALSYR ELDEAADNAA HAFLRLGIAP GDRVLLQLPN RASFAVALFG LMRAGAIPVM CLPGHRAAEL SHFIEVADAV GLVIPDSAGG FDYRALAVAL SQRHPRLRTV VVEGDAGPFA SWTSLLKSVE PQGPLPTVQT DTPALLLVSG GTTAAPKLIP RTHEDYVYNA TQAARVCGLT QDDVYLVALP AGHNFPLACP GLLGAISTGA TTVFLSDPSP ESAFATIARH RVSVTAVVPS LAQLWAQATA WEPVLPETLR LLQVGGAKLG ADDARAVRES LTQGLQQVFG MAEGLLCLTQ PGDPTDILDN SQGRPMCDAD EVRIVDEDGA DVPAGETGEL LVRGPYTLNG YYRADADNMR SFTVDGFYRS GDRVRALPGG YLEVTGRIKD VILRGGESIA ALDLESHLQT HPAVYAAAAV GLPDQYLGEI VCAAIVFKGK PVAAAELNQH LQARGAATHS RVDKLVAVSA LPLTAVGKID KRALLASLTG
NT Sequence
atgactcagc ccagatcgac caccaccgcg tcgtcagccg caacccccgc actggatggt ttcgtcccgt ttccctcggt acgcgccgac gaatatcgcc gggccgggta ctggacgggc aacaccgtcg aatcgctgct tcgcgtgtcc gcggccaggc ggccatgcca tccggctgtg atcgacgaac accgcgcgct gagctatcga gaactggacg aggcagccga caatgccgca cacgccttcc tccggctggg gatagcgccc ggcgatcggg tgctgctcca actccccaat cgggcatcgt ttgccgtcgc gctattcggc ttgatgcgtg ccggcgcaat acccgtgatg tgccttcccg ggcaccgggc cgccgaactg tcccacttca tcgaggtggc cgacgctgtc ggattggtta tccccgattc ggccggtgga ttcgattacc gcgcgctggc ggtcgcgctg tcccagcggc atccgcgcct gcgcacggtg gtcgtggagg gtgatgcggg ccccttcgca tcctggacgt cgctgctgaa aagtgttgag ccgcagggtc cgctacctac cgtgcagacg gacactcctg ccctactgct ggtgtccggc ggcaccaccg cagcacccaa gctgatccct cgcacccacg aggactacgt ctacaacgcc acacaggcag cacgggtctg cggtctgacc caggacgacg tttacctcgt ggcactgccc gccggccata atttccccct ggcttgcccg ggcttgcttg gtgcgataag caccggtgcg accacggttt tcctctcgga tccgagtccg gagtcggctt tcgcgaccat cgctcgccat cgagtgtcgg tgacggcagt ggtgccatcg cttgcacagc tatgggctca agccaccgcg tgggagccgg tgctgccgga aacactgagg ctgctccagg ttggtggcgc caaactcggc gccgacgacg cacgagcggt acgagagtcg ctcacccagg gactgcaaca ggttttcggc atggcagagg gcctgttatg cctcacacag ccaggcgatc ccaccgatat cctggataac agccagggcc gtcccatgtg cgacgcggac gaggtgcgca tagtcgacga ggacggcgcc gacgtccccg ccggagaaac cggggaactg ctcgtgcgtg ggccatacac cctcaacggg tactaccgcg ccgacgccga caacatgcgc tcgttcactg tcgacggttt ctatcgcagc ggtgaccggg tacgcgcact accgggcggt tatctggaag tcaccggacg catcaaggat gtcattctcc gtggcgggga atccatcgcc gcgttggatc tcgaatcgca cttgcagacc catccggcgg tctacgccgc agcggcggtg ggccttcccg atcagtatct tggcgagatt gtctgtgctg caattgtttt caagggaaaa ccggtcgccg ccgccgaact caatcagcat ctgcaagccc gtggcgccgc gacccattca cgcgtcgaca aactggtcgc cgtttccgca ctgccactga ccgctgtggg caagatcgac aagcgagcgc tgctggcctc cctgacgggc tag
Details for MyabA.00629.d.B2.GE40015
HARVESTED ON: 3/24/2016
SEQUENCED ON: 3/25/2016
EXPECTED MW: 57kDa
OBSERVED MW: 57kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 78
PERCENT COVERAGE: 66
Validated AA Sequence
MAHHHHHHMG FVPFPSVRAD EYRRAGYWTG NTVESLLRVS AARRPCHPAV IDEHRALSYR ELDEAADNAA HAFLRLGIAP GDRVLLQLPN RASFAVALFG LMRAGAIPVM CLPGHRAAEL SHFIEVADAV GLVIPDSAGG FDYRALAVAL SQRHPRLRTV VVEGDAGPFA SWTSLLKSVE PQGPLPTVQT DTPALLLVSG GTTAAPKLIP RTHEDYVYNA TQAARVCGLT QDDVYLVALP AGHNFPLACX XLAWCDKHRC DHGFPLGSES GVGFRDHRSP SSVGDGSGAI ACTAMGSSHR VGAGAAGNTE AAPGWWRQTR RRRRTSGTRV AHPGTATGFR HGRGPVMPHT ARRSHRYPG
Validated NT Sequence
tcgcttgtcg atcttgccca cagcggtcag tggcagtgcg gaaacggcga ccagtttgtc gacgcgtgaa tgggtcgcgg cgccacgggc ttgcagatgc tgattgagtt cggcggcggc gaccggtttt cccttgaaaa caattgcagc acagacaatc tcgccaagat actgatcggg aaggcccacc gccgctgcgg cgtagaccgc cggatgggtc tgcaagtgcg attcgagatc caacgcggcg atggattccc cgccacggag aatgacatcc ttgatgcgtc cggtgacttc cagataaccg cccggtagtg cgcgtacccg gtcaccgctg cgatagaaac cgtcgacagt gaacgagcgc atgttgtcgg cgtcggcgcg gtagtacccg ttgagggtgt atggcccacg cacgagcagt tccccggttt ctccggcggg gacgtcggcg ccgtcctcgt cgactatgcg cacctcgtcc gcgtcgcaca tgggacggcc ctggctgtta tccaggatat cggtgggatc gcctggctgt gtgaggcata acaggacctc tgccatgccg aaaacctgtt gcagtccctg ggtgagcgac tctcgtaccg ctcgtgcgtc gtcggcgccg agtttggcgc caccaacctg gagcagcctc agtgtttccg gcagcaccgg ctcccacgcg gtggcttgag cccatagctg tgcaagcgat ggcaccactg ccgtcaccga cactcgatgg cgagcgatgg tcgcgaaagc cgactccgga ctcggatccg agaggaaaac cgtggtcgca ccggtgctta tcgcaccaag caagccnnng gcaagccagg gggaaattat ggccggcggg cagtgccacg aggtaaacgt cgtcctgggt cagaccgcag acccgtgctg cctgtgtggc gttgtagacg tagtcctcgt gggtgcgagg gatcagcttg ggtgctgcgg tggtgccgcc ggacaccagc agtagggcag gagtgtccgt ctgcacggta ggtagcggac cctgcggctc aacacttttc agcagcgacg tccaggatgc gaaggggccc gcatcaccct ccacgaccac cgtgcgcagg cgcggatgcc gctgggacag cgcgaccgcc agcgcgcggt aatcgaatcc accggccgaa tcggggataa ccaatccgac agcgtcggcc acctcgatga agtgggacag ttcggcggcc cggtgcccgg gaaggcacat cacgggtatt gcgccggcac gcatcaagcc gaatagcgcg acggcaaacg atgcccgatt ggggagttgg agcagcaccc gatcgccggg cgctatcccc agccggagga aggcgtgtgc ggcattgtcg gctgcctcgt ccagttctcg atagctcagc gcgcggtgtt cgtcgatcac agccggatgg catggccgcc tggccgcgga cacgcgaagc agcgattcga cggtgttgcc cgtccagtac ccggcccggc gatattcgtc ggcgcgtacc gagggaaacg ggacgaaacc catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHGF VPFPSVRADE YRRAGYWTGN TVESLLRVSA ARRPCHPAVI DEHRALSYRE LDEAADNAAH AFLRLGIAPG DRVLLQLPNR ASFAVALFGL MRAGAIPVMC LPGHRAAELS HFIEVADAVG LVIPDSAGGF DYRALAVALS QRHPRLRTVV VEGDAGPFAS WTSLLKSVEP QGPLPTVQTD TPALLLVSGG TTAAPKLIPR THEDYVYNAT QAARVCGLTQ DDVYLVALPA GHNFPLACPG LLGAISTGAT TVFLSDPSPE SAFATIARHR VSVTAVVPSL AQLWAQATAW EPVLPETLRL LQVGGAKLGA DDARAVRESL TQGLQQVFGM AEGLLCLTQP GDPTDILDNS QGRPMCDADE VRIVDEDGAD VPAGETGELL VRGPYTLNGY YRADADNMRS FTVDGFYRSG DRVRALPGGY LEVTGRIKDV ILRGGESIAA LDLESHLQTH PAVYAAAAVG LPDQYLGEIV CAAIVFKGKP VAAAELNQHL QARGAATHSR VDKLVAVSAL PLTAVGKIDK RALLASLTG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggtttcg tcccgtttcc ctcggtacgc gccgacgaat atcgccgggc cgggtactgg acgggcaaca ccgtcgaatc gctgcttcgc gtgtccgcgg ccaggcggcc atgccatccg gctgtgatcg acgaacaccg cgcgctgagc tatcgagaac tggacgaggc agccgacaat gccgcacacg ccttcctccg gctggggata gcgcccggcg atcgggtgct gctccaactc cccaatcggg catcgtttgc cgtcgcgcta ttcggcttga tgcgtgccgg cgcaataccc gtgatgtgcc ttcccgggca ccgggccgcc gaactgtccc acttcatcga ggtggccgac gctgtcggat tggttatccc cgattcggcc ggtggattcg attaccgcgc gctggcggtc gcgctgtccc agcggcatcc gcgcctgcgc acggtggtcg tggagggtga tgcgggcccc ttcgcatcct ggacgtcgct gctgaaaagt gttgagccgc agggtccgct acctaccgtg cagacggaca ctcctgccct actgctggtg tccggcggca ccaccgcagc acccaagctg atccctcgca cccacgagga ctacgtctac aacgccacac aggcagcacg ggtctgcggt ctgacccagg acgacgttta cctcgtggca ctgcccgccg gccataattt ccccctggct tgcccgggct tgcttggtgc gataagcacc ggtgcgacca cggttttcct ctcggatccg agtccggagt cggctttcgc gaccatcgct cgccatcgag tgtcggtgac ggcagtggtg ccatcgcttg cacagctatg ggctcaagcc accgcgtggg agccggtgct gccggaaaca ctgaggctgc tccaggttgg tggcgccaaa ctcggcgccg acgacgcacg agcggtacga gagtcgctca cccagggact gcaacaggtt ttcggcatgg cagagggcct gttatgcctc acacagccag gcgatcccac cgatatcctg gataacagcc agggccgtcc catgtgcgac gcggacgagg tgcgcatagt cgacgaggac ggcgccgacg tccccgccgg agaaaccggg gaactgctcg tgcgtgggcc atacaccctc aacgggtact accgcgccga cgccgacaac atgcgctcgt tcactgtcga cggtttctat cgcagcggtg accgggtacg cgcactaccg ggcggttatc tggaagtcac cggacgcatc aaggatgtca ttctccgtgg cggggaatcc atcgccgcgt tggatctcga atcgcacttg cagacccatc cggcggtcta cgccgcagcg gcggtgggcc ttcccgatca gtatcttggc gagattgtct gtgctgcaat tgttttcaag ggaaaaccgg tcgccgccgc cgaactcaat cagcatctgc aagcccgtgg cgccgcgacc cattcacgcg tcgacaaact ggtcgccgtt tccgcactgc cactgaccgc tgtgggcaag atcgacaagc gagcgctgct ggcctccctg acgggctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t