MyabA.00587.f

Putative transcriptional regulatory protein PrrA

CENTER ID: MyabA.00587.f
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00587.f.A1.GE30321 Full length( MyabA.00587.f ) 1 236
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00587.f.A1.PS00920 Full length( MyabA.00587.f ) 1 236

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.1961
RefSeq: YP_001702663.1
UniProt: B1MNV8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTQETTARHK VLMVDDDPDV RNSVARGLRH SGFDVRVAVD GRDALTQLAA DEPDALVLDV QMPELDGVAV VTALRALGND IPICVLSARD TVNDRIAGLE AGADDYLTKP FDLGELVARL RALLRRRGAG SGGLADSAIT VGQITVDVSR RLVFVNGERV ELSKREFDLL VVLAENTGVV LSRTRLLELV WGYDFDVETN VADVFVSYLR RKLEAGGAAR VIHTVRGVGY VMREEP
NT Sequence
ATGACACAGG AAACTACCGC CCGTCACAAG GTGTTGATGG TCGATGACGA CCCGGACGTT CGGAACTCGG TAGCCCGCGG GCTACGCCAC TCGGGTTTCG ACGTCCGGGT CGCCGTTGAT GGCCGCGACG CGCTCACCCA GCTCGCGGCC GATGAGCCCG ATGCCCTGGT ACTCGACGTG CAGATGCCCG AGCTGGACGG GGTTGCGGTG GTCACCGCTC TGCGGGCCCT GGGTAACGAC ATTCCGATCT GCGTGCTCAG TGCACGCGAC ACCGTCAACG ACCGAATCGC CGGCTTGGAG GCCGGTGCCG ACGACTACTT GACCAAACCC TTTGACCTCG GCGAGCTGGT GGCTCGCCTG CGTGCGCTGC TGCGCCGTCG CGGCGCCGGT TCGGGCGGCC TCGCCGATTC CGCCATCACC GTCGGACAGA TCACCGTGGA CGTGTCGCGC CGCTTGGTCT TTGTCAACGG AGAACGTGTC GAGCTGAGTA AGCGGGAGTT CGATCTGCTG GTGGTGCTGG CCGAGAACAC CGGTGTGGTG CTGAGCCGCA CCCGGCTGCT GGAATTGGTG TGGGGGTACG ACTTTGACGT CGAGACCAAT GTTGCCGATG TCTTCGTCAG CTATCTGCGC CGCAAGCTGG AAGCTGGGGG AGCCGCGCGA GTCATCCATA CCGTCAGGGG AGTCGGCTAC GTGATGCGGG AAGAGCCG
Details for MyabA.00587.f.A1.GE30321
HARVESTED ON: 9/8/2010
SEQUENCED ON: 9/17/2010
EXPECTED MW: 27kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTQETTARH KVLMVDDDPD VRNSVARGLR HSGFDVRVAV DGRDALTQLA ADEPDALVLD VQMPELDGVA VVTALRALGN DIPICVLSAR DTVNDRIAGL EAGADDYLTK PFDLGELVAR LRALLRRRGA GSGGLADSAI TVGQITVDVS RRLVFVNGER VELSKREFDL LVVLAENTGV VLSRTRLLEL VWGYDFDVET NVADVFVSYL RRKLEAGGAA RVIHTVRGVG YVMRE
Validated NT Sequence
cttcccgcat cacgtagccg actcccctga cggtatggat gactcgcgcg gctcccccag cttccagctt gcggcgcaga tagctgacga agacatcggc aacattggtc tcgacgtcaa agtcgtaccc ccacaccaat tccagcagcc gggtgcggct cagcaccaca ccggtgttct cggccagcac caccagcaga tcgaactccc gcttactcag ctcgacacgt tctccgttga caaagaccaa gcggcgcgac acgtccacgg tgatctgtcc gacggtgatg gcggaatcgg cgaggccgcc cgaaccggcg ccgcgacggc gcagcagcgc acgcaggcga gccaccagct cgccgaggtc aaagggtttg gtcaagtagt cgtcggcacc ggcctccaag ccggcgattc ggtcgttgac ggtgtcgcgt gcactgagca cgcagatcgg aatgtcgtta cccagggccc gcagagcggt gaccaccgca accccgtcca gctcgggcat ctgcacgtcg agtaccaggg catcgggctc atcggccgcg agctgggtga gcgcgtcgcg gccatcaacg gcgacccgga cgtcgaaacc cgagtggcgt agcccgcggg ctaccgagtt ccgaacgtcc gggtcgtcat cgaccatcaa caccttgtga cgggcggtag tttcctgtgt catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTQETTARH KVLMVDDDPD VRNSVARGLR HSGFDVRVAV DGRDALTQLA ADEPDALVLD VQMPELDGVA VVTALRALGN DIPICVLSAR DTVNDRIAGL EAGADDYLTK PFDLGELVAR LRALLRRRGA GSGGLADSAI TVGQITVDVS RRLVFVNGER VELSKREFDL LVVLAENTGV VLSRTRLLEL VWGYDFDVET NVADVFVSYL RRKLEAGGAA RVIHTVRGVG YVMREEP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacacaggaa actaccgccc gtcacaaggt gttgatggtc gatgacgacc cggacgttcg gaactcggta gcccgcgggc tacgccactc gggtttcgac gtccgggtcg ccgttgatgg ccgcgacgcg ctcacccagc tcgcggccga tgagcccgat gccctggtac tcgacgtgca gatgcccgag ctggacgggg ttgcggtggt caccgctctg cgggccctgg gtaacgacat tccgatctgc gtgctcagtg cacgcgacac cgtcaacgac cgaatcgccg gcttggaggc cggtgccgac gactacttga ccaaaccctt tgacctcggc gagctggtgg ctcgcctgcg tgcgctgctg cgccgtcgcg gcgccggttc gggcggcctc gccgattccg ccatcaccgt cggacagatc accgtggacg tgtcgcgccg cttggtcttt gtcaacggag aacgtgtcga gctgagtaag cgggagttcg atctgctggt ggtgctggcc gagaacaccg gtgtggtgct gagccgcacc cggctgctgg aattggtgtg ggggtacgac tttgacgtcg agaccaatgt tgccgatgtc ttcgtcagct atctgcgccg caagctggaa gctgggggag ccgcgcgagt catccatacc gtcaggggag tcggctacgt gatgcgggaa gagccgtaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for MyabA.00587.f.A1.PS00920
PURIFICATION DATe: 12/19/2010
CONCENTRATION: 11.48mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTQETTARH KVLMVDDDPD VRNSVARGLR HSGFDVRVAV DGRDALTQLA ADEPDALVLD VQMPELDGVA VVTALRALGN DIPICVLSAR DTVNDRIAGL EAGADDYLTK PFDLGELVAR LRALLRRRGA GSGGLADSAI TVGQITVDVS RRLVFVNGER VELSKREFDL LVVLAENTGV VLSRTRLLEL VWGYDFDVET NVADVFVSYL RRKLEAGGAA RVIHTVRGVG YVMRE
Validated NT Sequence
cttcccgcat cacgtagccg actcccctga cggtatggat gactcgcgcg gctcccccag cttccagctt gcggcgcaga tagctgacga agacatcggc aacattggtc tcgacgtcaa agtcgtaccc ccacaccaat tccagcagcc gggtgcggct cagcaccaca ccggtgttct cggccagcac caccagcaga tcgaactccc gcttactcag ctcgacacgt tctccgttga caaagaccaa gcggcgcgac acgtccacgg tgatctgtcc gacggtgatg gcggaatcgg cgaggccgcc cgaaccggcg ccgcgacggc gcagcagcgc acgcaggcga gccaccagct cgccgaggtc aaagggtttg gtcaagtagt cgtcggcacc ggcctccaag ccggcgattc ggtcgttgac ggtgtcgcgt gcactgagca cgcagatcgg aatgtcgtta cccagggccc gcagagcggt gaccaccgca accccgtcca gctcgggcat ctgcacgtcg agtaccaggg catcgggctc atcggccgcg agctgggtga gcgcgtcgcg gccatcaacg gcgacccgga cgtcgaaacc cgagtggcgt agcccgcggg ctaccgagtt ccgaacgtcc gggtcgtcat cgaccatcaa caccttgtga cgggcggtag tttcctgtgt catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTQETTARH KVLMVDDDPD VRNSVARGLR HSGFDVRVAV DGRDALTQLA ADEPDALVLD VQMPELDGVA VVTALRALGN DIPICVLSAR DTVNDRIAGL EAGADDYLTK PFDLGELVAR LRALLRRRGA GSGGLADSAI TVGQITVDVS RRLVFVNGER VELSKREFDL LVVLAENTGV VLSRTRLLEL VWGYDFDVET NVADVFVSYL RRKLEAGGAA RVIHTVRGVG YVMREEP
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACACAGGAA ACTACCGCCC GTCACAAGGT GTTGATGGTC GATGACGACC CGGACGTTCG GAACTCGGTA GCCCGCGGGC TACGCCACTC GGGTTTCGAC GTCCGGGTCG CCGTTGATGG CCGCGACGCG CTCACCCAGC TCGCGGCCGA TGAGCCCGAT GCCCTGGTAC TCGACGTGCA GATGCCCGAG CTGGACGGGG TTGCGGTGGT CACCGCTCTG CGGGCCCTGG GTAACGACAT TCCGATCTGC GTGCTCAGTG CACGCGACAC CGTCAACGAC CGAATCGCCG GCTTGGAGGC CGGTGCCGAC GACTACTTGA CCAAACCCTT TGACCTCGGC GAGCTGGTGG CTCGCCTGCG TGCGCTGCTG CGCCGTCGCG GCGCCGGTTC GGGCGGCCTC GCCGATTCCG CCATCACCGT CGGACAGATC ACCGTGGACG TGTCGCGCCG CTTGGTCTTT GTCAACGGAG AACGTGTCGA GCTGAGTAAG CGGGAGTTCG ATCTGCTGGT GGTGCTGGCC GAGAACACCG GTGTGGTGCT GAGCCGCACC CGGCTGCTGG AATTGGTGTG GGGGTACGAC TTTGACGTCG AGACCAATGT TGCCGATGTC TTCGTCAGCT ATCTGCGCCG CAAGCTGGAA GCTGGGGGAG CCGCGCGAGT CATCCATACC GTCAGGGGAG TCGGCTACGT GATGCGGGAA GAGCCGTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA