MyabA.00587.c

Probable transcriptional regulatory protein KdpE

CENTER ID: MyabA.00587.c
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00587.c.A1.GE30318 Full length( MyabA.00587.c ) 1 224
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00587.c.A1.PW30195 Full length( MyabA.00587.c ) 1 224
MyabA.00587.c.A1.PW31349 Full length( MyabA.00587.c ) 1 224

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.3308
RefSeq: YP_001703980.1
UniProt: B1MDK7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTRVLVVDDE PQILRAMRIN LSARGYEVVT ASSGAGALRA AAETQPEVVI LDLGLPDMDG TEVLAGLRGW TDVPVIVLSA RTDSADKVEA LDAGADDYVT KPFGMDEFLA RLRAAVRRGT PDSAEPVVHT ETFDVDLSSH KVIRDGREVH LTPTEWSMLA VLVRNRGKLV GQKELLHEVW GPAYSSETHY LRVYLAQLRR KLEIDPAHPK HLLTEAGMGY RFQE
NT Sequence
ATGACCCGGG TGCTGGTGGT GGACGACGAG CCGCAGATTC TGCGCGCCAT GCGCATAAAC CTGTCGGCAC GCGGATATGA GGTAGTGACA GCCTCCTCCG GCGCCGGAGC GCTGCGCGCG GCGGCCGAGA CCCAGCCCGA AGTGGTCATT CTGGACCTCG GATTGCCCGA TATGGACGGC ACCGAGGTAC TCGCCGGGCT GCGTGGCTGG ACCGACGTGC CGGTGATCGT GCTCTCGGCC CGCACCGATT CCGCGGACAA GGTGGAGGCG TTGGACGCCG GTGCCGACGA TTACGTGACC AAACCCTTCG GCATGGATGA GTTTTTGGCC CGGCTCCGGG CCGCGGTACG CCGAGGCACC CCTGACAGTG CCGAGCCGGT GGTCCATACC GAGACCTTCG ACGTGGATCT TTCCTCGCAC AAGGTGATCC GCGACGGCAG AGAGGTACAC CTGACACCCA CCGAATGGTC GATGCTGGCT GTGCTGGTGC GCAATCGTGG AAAGCTGGTG GGGCAGAAGG AGCTGTTGCA CGAGGTATGG GGCCCGGCGT ACAGCTCCGA AACTCATTAC TTGCGTGTGT ATCTCGCGCA GCTGCGGCGC AAGCTGGAAA TCGACCCGGC GCACCCCAAG CATCTGCTCA CCGAGGCGGG GATGGGCTAT CGCTTCCAGG AG
Details for MyabA.00587.c.A1.GE30318
HARVESTED ON: 9/8/2010
SEQUENCED ON: 9/17/2010
EXPECTED MW: 27kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTRVLVVDD EPQILRAMRI NLSARGYEVV TASSGAGALR AAAETQPEVV ILDLGLPDMD GTEVLAGLRG WTDVPVIVLS ARTDSADKVE ALDAGADDYV TKPFGMDEFL ARLRAAVRRG TPDSAEPVVH TETFDVDLSS HKVIRDGREV HLTPTEWSML AVLVRNRGKL VGQKELLHEV WGPAYSSETH YLRVYLAQLR RKLEIDPAHP KHLLTEAGMG YR
Validated NT Sequence
aagcgatagc ccatccccgc ctcggtgagc agatgcttgg ggtgcgccgg gtcgatttcc agcttgcgcc gcagctgcgc gagatacaca cgcaagtaat gagtttcgga gctgtacgcc gggccccata cctcgtgcaa cagctccttc tgccccacca gctttccacg attgcgcacc agcacagcca gcatcgacca ttcggtgggt gtcaggtgta cctctctgcc gtcgcggatc accttgtgcg aggaaagatc cacgtcgaag gtctcggtat ggaccaccgg ctcggcactg tcaggggtgc ctcggcgtac cgcggcccgg agccgggcca aaaactcatc catgccgaag ggtttggtca cgtaatcgtc ggcaccggcg tccaacgcct ccaccttgtc cgcggaatcg gtgcgggccg agagcacgat caccggcacg tcggtccagc cacgcagccc ggcgagtacc tcggtgccgt ccatatcggg caatccgagg tccagaatga ccacttcggg ctgggtctcg gccgccgcgc gcagcgctcc ggcgccggag gaggctgtca ctacctcata tccgcgtgcc gacaggttta tgcgcatggc gcgcagaatc tgcggctcgt cgtccaccac cagcacccgg gtcatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTRVLVVDD EPQILRAMRI NLSARGYEVV TASSGAGALR AAAETQPEVV ILDLGLPDMD GTEVLAGLRG WTDVPVIVLS ARTDSADKVE ALDAGADDYV TKPFGMDEFL ARLRAAVRRG TPDSAEPVVH TETFDVDLSS HKVIRDGREV HLTPTEWSML AVLVRNRGKL VGQKELLHEV WGPAYSSETH YLRVYLAQLR RKLEIDPAHP KHLLTEAGMG YRFQE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacccgggtg ctggtggtgg acgacgagcc gcagattctg cgcgccatgc gcataaacct gtcggcacgc ggatatgagg tagtgacagc ctcctccggc gccggagcgc tgcgcgcggc ggccgagacc cagcccgaag tggtcattct ggacctcgga ttgcccgata tggacggcac cgaggtactc gccgggctgc gtggctggac cgacgtgccg gtgatcgtgc tctcggcccg caccgattcc gcggacaagg tggaggcgtt ggacgccggt gccgacgatt acgtgaccaa acccttcggc atggatgagt ttttggcccg gctccgggcc gcggtacgcc gaggcacccc tgacagtgcc gagccggtgg tccataccga gaccttcgac gtggatcttt cctcgcacaa ggtgatccgc gacggcagag aggtacacct gacacccacc gaatggtcga tgctggctgt gctggtgcgc aatcgtggaa agctggtggg gcagaaggag ctgttgcacg aggtatgggg cccggcgtac agctccgaaa ctcattactt gcgtgtgtat ctcgcgcagc tgcggcgcaa gctggaaatc gacccggcgc accccaagca tctgctcacc gaggcgggga tgggctatcg cttccaggag taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for MyabA.00587.c.A1.PW30195
PURIFICATION DATe: 12/13/2010
CONCENTRATION: 10.76mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTRVLVVDD EPQILRAMRI NLSARGYEVV TASSGAGALR AAAETQPEVV ILDLGLPDMD GTEVLAGLRG WTDVPVIVLS ARTDSADKVE ALDAGADDYV TKPFGMDEFL ARLRAAVRRG TPDSAEPVVH TETFDVDLSS HKVIRDGREV HLTPTEWSML AVLVRNRGKL VGQKELLHEV WGPAYSSETH YLRVYLAQLR RKLEIDPAHP KHLLTEAGMG YR
Validated NT Sequence
aagcgatagc ccatccccgc ctcggtgagc agatgcttgg ggtgcgccgg gtcgatttcc agcttgcgcc gcagctgcgc gagatacaca cgcaagtaat gagtttcgga gctgtacgcc gggccccata cctcgtgcaa cagctccttc tgccccacca gctttccacg attgcgcacc agcacagcca gcatcgacca ttcggtgggt gtcaggtgta cctctctgcc gtcgcggatc accttgtgcg aggaaagatc cacgtcgaag gtctcggtat ggaccaccgg ctcggcactg tcaggggtgc ctcggcgtac cgcggcccgg agccgggcca aaaactcatc catgccgaag ggtttggtca cgtaatcgtc ggcaccggcg tccaacgcct ccaccttgtc cgcggaatcg gtgcgggccg agagcacgat caccggcacg tcggtccagc cacgcagccc ggcgagtacc tcggtgccgt ccatatcggg caatccgagg tccagaatga ccacttcggg ctgggtctcg gccgccgcgc gcagcgctcc ggcgccggag gaggctgtca ctacctcata tccgcgtgcc gacaggttta tgcgcatggc gcgcagaatc tgcggctcgt cgtccaccac cagcacccgg gtcatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTRVLVVDD EPQILRAMRI NLSARGYEVV TASSGAGALR AAAETQPEVV ILDLGLPDMD GTEVLAGLRG WTDVPVIVLS ARTDSADKVE ALDAGADDYV TKPFGMDEFL ARLRAAVRRG TPDSAEPVVH TETFDVDLSS HKVIRDGREV HLTPTEWSML AVLVRNRGKL VGQKELLHEV WGPAYSSETH YLRVYLAQLR RKLEIDPAHP KHLLTEAGMG YRFQE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCCGGGTG CTGGTGGTGG ACGACGAGCC GCAGATTCTG CGCGCCATGC GCATAAACCT GTCGGCACGC GGATATGAGG TAGTGACAGC CTCCTCCGGC GCCGGAGCGC TGCGCGCGGC GGCCGAGACC CAGCCCGAAG TGGTCATTCT GGACCTCGGA TTGCCCGATA TGGACGGCAC CGAGGTACTC GCCGGGCTGC GTGGCTGGAC CGACGTGCCG GTGATCGTGC TCTCGGCCCG CACCGATTCC GCGGACAAGG TGGAGGCGTT GGACGCCGGT GCCGACGATT ACGTGACCAA ACCCTTCGGC ATGGATGAGT TTTTGGCCCG GCTCCGGGCC GCGGTACGCC GAGGCACCCC TGACAGTGCC GAGCCGGTGG TCCATACCGA GACCTTCGAC GTGGATCTTT CCTCGCACAA GGTGATCCGC GACGGCAGAG AGGTACACCT GACACCCACC GAATGGTCGA TGCTGGCTGT GCTGGTGCGC AATCGTGGAA AGCTGGTGGG GCAGAAGGAG CTGTTGCACG AGGTATGGGG CCCGGCGTAC AGCTCCGAAA CTCATTACTT GCGTGTGTAT CTCGCGCAGC TGCGGCGCAA GCTGGAAATC GACCCGGCGC ACCCCAAGCA TCTGCTCACC GAGGCGGGGA TGGGCTATCG CTTCCAGGAG TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA
Details for MyabA.00587.c.A1.PW31349
PURIFICATION DATe: 12/13/2010
CONCENTRATION: 3.89mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTRVLVVDD EPQILRAMRI NLSARGYEVV TASSGAGALR AAAETQPEVV ILDLGLPDMD GTEVLAGLRG WTDVPVIVLS ARTDSADKVE ALDAGADDYV TKPFGMDEFL ARLRAAVRRG TPDSAEPVVH TETFDVDLSS HKVIRDGREV HLTPTEWSML AVLVRNRGKL VGQKELLHEV WGPAYSSETH YLRVYLAQLR RKLEIDPAHP KHLLTEAGMG YR
Validated NT Sequence
aagcgatagc ccatccccgc ctcggtgagc agatgcttgg ggtgcgccgg gtcgatttcc agcttgcgcc gcagctgcgc gagatacaca cgcaagtaat gagtttcgga gctgtacgcc gggccccata cctcgtgcaa cagctccttc tgccccacca gctttccacg attgcgcacc agcacagcca gcatcgacca ttcggtgggt gtcaggtgta cctctctgcc gtcgcggatc accttgtgcg aggaaagatc cacgtcgaag gtctcggtat ggaccaccgg ctcggcactg tcaggggtgc ctcggcgtac cgcggcccgg agccgggcca aaaactcatc catgccgaag ggtttggtca cgtaatcgtc ggcaccggcg tccaacgcct ccaccttgtc cgcggaatcg gtgcgggccg agagcacgat caccggcacg tcggtccagc cacgcagccc ggcgagtacc tcggtgccgt ccatatcggg caatccgagg tccagaatga ccacttcggg ctgggtctcg gccgccgcgc gcagcgctcc ggcgccggag gaggctgtca ctacctcata tccgcgtgcc gacaggttta tgcgcatggc gcgcagaatc tgcggctcgt cgtccaccac cagcacccgg gtcatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTRVLVVDD EPQILRAMRI NLSARGYEVV TASSGAGALR AAAETQPEVV ILDLGLPDMD GTEVLAGLRG WTDVPVIVLS ARTDSADKVE ALDAGADDYV TKPFGMDEFL ARLRAAVRRG TPDSAEPVVH TETFDVDLSS HKVIRDGREV HLTPTEWSML AVLVRNRGKL VGQKELLHEV WGPAYSSETH YLRVYLAQLR RKLEIDPAHP KHLLTEAGMG YRFQE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCCGGGTG CTGGTGGTGG ACGACGAGCC GCAGATTCTG CGCGCCATGC GCATAAACCT GTCGGCACGC GGATATGAGG TAGTGACAGC CTCCTCCGGC GCCGGAGCGC TGCGCGCGGC GGCCGAGACC CAGCCCGAAG TGGTCATTCT GGACCTCGGA TTGCCCGATA TGGACGGCAC CGAGGTACTC GCCGGGCTGC GTGGCTGGAC CGACGTGCCG GTGATCGTGC TCTCGGCCCG CACCGATTCC GCGGACAAGG TGGAGGCGTT GGACGCCGGT GCCGACGATT ACGTGACCAA ACCCTTCGGC ATGGATGAGT TTTTGGCCCG GCTCCGGGCC GCGGTACGCC GAGGCACCCC TGACAGTGCC GAGCCGGTGG TCCATACCGA GACCTTCGAC GTGGATCTTT CCTCGCACAA GGTGATCCGC GACGGCAGAG AGGTACACCT GACACCCACC GAATGGTCGA TGCTGGCTGT GCTGGTGCGC AATCGTGGAA AGCTGGTGGG GCAGAAGGAG CTGTTGCACG AGGTATGGGG CCCGGCGTAC AGCTCCGAAA CTCATTACTT GCGTGTGTAT CTCGCGCAGC TGCGGCGCAA GCTGGAAATC GACCCGGCGC ACCCCAAGCA TCTGCTCACC GAGGCGGGGA TGGGCTATCG CTTCCAGGAG TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA