MyabA.00572.a

Putative ABC transporter, ATP-binding protein

CENTER ID: MyabA.00572.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00572.a.A1.GE30315 Full length( MyabA.00572.a ) 1 301
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.1398
RefSeq: YP_001702099.1
UniProt: B1MLL8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTYPNLAVEI RGLTKLFGKT KALDGLDLSV AHGEVAGFLG PNGAGKSTTI RVLLGLLRAD AGTVQLLGGD PWVNAVDLHR LIAYVPGDVT LWPNLTGGQA IDFLTRMLGT EHISIRRRNE LIERFELDPT KKARTYSKGN RQKVALIAAF CSDADLFILD EPTSGLDPLM ETVFQECVAE VSTRGCAVLL SSHILAEVEK LCHRVTIVRA GRTVLSGSLA ELRHFMRTSV TVRTLANPSG LKSIPQLRDV TIGGNRASFT VDRSDLDRTM EQLAGLGIVD LSVAPASLEE LFMREYQGAS R
NT Sequence
GTGACGTATC CGAATTTGGC GGTCGAAATA CGAGGACTGA CGAAGCTTTT CGGAAAAACC AAGGCACTCG ACGGCCTGGA TTTGTCCGTC GCACATGGCG AAGTGGCGGG CTTCCTCGGA CCGAACGGCG CCGGCAAGTC CACCACAATC CGGGTACTAC TCGGCCTGCT GCGCGCCGAT GCCGGCACCG TACAGCTCCT CGGCGGCGAC CCCTGGGTCA ATGCCGTCGA CCTGCACCGA CTGATCGCCT ACGTACCGGG AGATGTCACC CTGTGGCCCA ACCTCACCGG CGGGCAGGCC ATCGACTTCC TCACCAGAAT GCTTGGTACC GAACATATTT CAATCCGCCG GCGCAATGAA CTCATCGAGC GCTTCGAATT GGACCCCACC AAGAAGGCGC GCACATATTC CAAGGGAAAT CGTCAGAAGG TGGCGCTGAT AGCCGCATTC TGTTCGGACG CCGATCTGTT CATCCTCGAC GAACCGACCT CAGGACTGGA TCCCCTCATG GAAACGGTCT TTCAAGAGTG CGTGGCCGAG GTATCGACAC GCGGATGTGC GGTACTGCTT TCCAGCCACA TTCTGGCCGA GGTGGAAAAG CTCTGTCACC GGGTCACCAT CGTGCGCGCC GGCCGTACCG TGCTCTCGGG TTCATTGGCC GAGCTACGGC ACTTCATGCG GACGTCGGTC ACGGTGCGCA CCTTGGCCAA TCCCTCAGGA CTGAAATCGA TTCCACAGTT GCGCGACGTC ACCATCGGAG GCAACCGCGC ATCCTTCACC GTGGATCGCA GCGATCTTGA CCGCACGATG GAACAATTGG CCGGTCTCGG GATCGTCGAC CTGAGCGTGG CCCCCGCCTC CCTCGAAGAA TTGTTCATGC GCGAGTACCA GGGCGCCTCG CGG
Details for MyabA.00572.a.A1.GE30315
HARVESTED ON: 9/8/2010
SEQUENCED ON: 9/17/2010
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 81
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTYPNLAV EIRGLTKLFG KTKALDGLDL SVAHGEVAGF LGPNGAGKST TIRVLLGLLR ADAGTVQLLG GDPWVNAVDL HRLIAYVPGD VTLWPNLTGG QAIDFLTRML GTEHISIRRR NELIERFELD PTKKARTYSK GNRQKVALIA AFCSDADLFI LDEPTSGLDP LMETVFQECV AEVSTRGCAV LLSSHILAEV EKLCHRVTIV RAGRTVLSGS LAELRHFMRT SVTVRTLANP SX
Validated NT Sequence
ttntttactt taagaaggag atataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atggtgacgt atccgaattt ggcggtcgaa atacgaggac tgacgaagct tttcggaaaa accaaggcac tcgacggcct ggatttgtcc gtcgcacatg gcgaagtggc gggcttcctc ggaccgaacg gcgccggcaa gtccaccaca atccgggtac tactcggcct gctgcgcgcc gatgccggca ccgtacagct cctcggcggc gacccctggg tcaatgccgt cgacctgcac cgactgatcg cctacgtacc gggagatgtc accctgtggc ccaacctcac cggcgggcag gccatcgact tcctcaccag aatgcttggt accgaacata tttcaatccg ccggcgcaat gaactcatcg agcgcttcga attggacccc accaagaagg cgcgcacata ttccaaggga aatcgtcaga aggtggcgct gatagccgca ttctgttcgg acgccgatct gttcatcctc gacgaaccga cctcaggact ggatcccctc atggaaacgg tctttcaaga gtgcgtggcc gaggtatcga cacgcggatg tgcggtactg ctttccagcc acattctggc cgaggtggaa aagctctgtc accgggtcac catcgtgcgc gccggccgta ccgtgctctc gggttcattg gccgagctac ggcacttcat gcggacgtcg gtcacggtgc gcaccttggc caatccctcn nnctgaaatc gattccacag ttgcgcgacg tcaccatcgg angcaaccgc gcatccttca ccgtggatcg cagcgatctt gaccgcacga tggaacaatt ggnccggtct cgggatcgtc nacctgagcg tggcccccgc cntccctcga agaattgttc atgcgcgagt accnnnnnnc tcgnggtann annagcacga ananttctgc agccnagctn nncgagnatn nnnctgctan caaannncga aannnagctg annnnnntgc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTYPNLAV EIRGLTKLFG KTKALDGLDL SVAHGEVAGF LGPNGAGKST TIRVLLGLLR ADAGTVQLLG GDPWVNAVDL HRLIAYVPGD VTLWPNLTGG QAIDFLTRML GTEHISIRRR NELIERFELD PTKKARTYSK GNRQKVALIA AFCSDADLFI LDEPTSGLDP LMETVFQECV AEVSTRGCAV LLSSHILAEV EKLCHRVTIV RAGRTVLSGS LAELRHFMRT SVTVRTLANP SGLKSIPQLR DVTIGGNRAS FTVDRSDLDR TMEQLAGLGI VDLSVAPASL EELFMREYQG ASR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgacgtat ccgaatttgg cggtcgaaat acgaggactg acgaagcttt tcggaaaaac caaggcactc gacggcctgg atttgtccgt cgcacatggc gaagtggcgg gcttcctcgg accgaacggc gccggcaagt ccaccacaat ccgggtacta ctcggcctgc tgcgcgccga tgccggcacc gtacagctcc tcggcggcga cccctgggtc aatgccgtcg acctgcaccg actgatcgcc tacgtaccgg gagatgtcac cctgtggccc aacctcaccg gcgggcaggc catcgacttc ctcaccagaa tgcttggtac cgaacatatt tcaatccgcc ggcgcaatga actcatcgag cgcttcgaat tggaccccac caagaaggcg cgcacatatt ccaagggaaa tcgtcagaag gtggcgctga tagccgcatt ctgttcggac gccgatctgt tcatcctcga cgaaccgacc tcaggactgg atcccctcat ggaaacggtc tttcaagagt gcgtggccga ggtatcgaca cgcggatgtg cggtactgct ttccagccac attctggccg aggtggaaaa gctctgtcac cgggtcacca tcgtgcgcgc cggccgtacc gtgctctcgg gttcattggc cgagctacgg cacttcatgc ggacgtcggt cacggtgcgc accttggcca atccctcagg actgaaatcg attccacagt tgcgcgacgt caccatcgga ggcaaccgcg catccttcac cgtggatcgc agcgatcttg accgcacgat ggaacaattg gccggtctcg ggatcgtcga cctgagcgtg gcccccgcct ccctcgaaga attgttcatg cgcgagtacc agggcgcctc gcggtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa