MyabA.00556.a

Succinyl-CoA ligase [ADP-forming] subunit alpha

CENTER ID: MyabA.00556.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00556.a.A1.GE30314 Full length( MyabA.00556.a ) 1 300
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.1094
RefSeq: YP_001701801.1
UniProt: B1MJJ1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSIFLNKDSK VIVQGITGGE GTKHTALMLK AGTQVVGGVN ARKAGTTVSH VDKSGQSVDL PVFGSVAEAI KETGADVSIA FVPPAFSKDA IIEAIDAEIP LLVVITEGIP VQDSAYAWAY NVEKGNKTRI IGPNCPGIIT PGEALVGITP NNITGTGPVG LVSKSGTLTY QMMYELRDFG FSTAIGIGGD PVIGTTHIDA IEAFEKDPET KIIVMIGEIG GDAEERAADY IKANVSKPVV GYVAGFTAPE GKTMGHAGAI VSGSSGTAQA KKEALEAAGV KVGKTPSETA KLAREILQSL
NT Sequence
ATGTCTATCT TTCTGAACAA GGATTCGAAG GTCATCGTCC AGGGCATCAC CGGTGGTGAG GGCACCAAGC ACACCGCGCT GATGCTCAAG GCCGGTACTC AGGTTGTCGG CGGCGTCAAC GCCCGCAAGG CCGGAACCAC CGTGTCGCAT GTGGATAAGA GCGGTCAAAG TGTCGACTTA CCCGTGTTCG GCAGTGTCGC CGAGGCCATC AAGGAGACCG GGGCCGACGT GTCGATCGCC TTCGTGCCGC CGGCCTTCTC CAAGGACGCC ATCATCGAGG CAATCGACGC CGAGATCCCG CTGCTCGTTG TCATCACTGA GGGAATCCCG GTGCAGGACA GCGCGTATGC GTGGGCCTAC AACGTCGAGA AGGGCAACAA GACCCGCATC ATCGGGCCCA ACTGCCCCGG CATCATCACC CCCGGCGAGG CGCTGGTGGG TATCACTCCT AACAACATCA CCGGCACCGG TCCGGTCGGT CTGGTGTCCA AGTCCGGCAC CCTGACCTAC CAGATGATGT ACGAGCTGCG CGATTTCGGC TTCTCCACCG CCATCGGTAT CGGCGGCGAC CCCGTCATCG GCACCACCCA CATCGACGCC ATCGAGGCGT TCGAGAAGGA CCCGGAGACC AAGATCATCG TCATGATCGG TGAGATCGGT GGCGACGCCG AGGAGCGTGC GGCCGACTAC ATCAAGGCCA ACGTGTCCAA GCCCGTCGTC GGCTACGTTG CGGGCTTCAC CGCCCCCGAG GGCAAGACCA TGGGCCACGC CGGTGCCATC GTGTCCGGCA GCTCGGGTAC CGCCCAGGCC AAGAAGGAGG CCCTCGAGGC CGCCGGTGTG AAGGTCGGCA AGACGCCGTC CGAGACGGCC AAGCTGGCGC GAGAGATCCT GCAGAGCCTG
Details for MyabA.00556.a.A1.GE30314
HARVESTED ON: 9/8/2010
SEQUENCED ON: 9/17/2010
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSIFLNKDS KVIVQGITGG EGTKHTALML KAGTQVVGGV NARKAGTTVS HVDKSGQSVD LPVFGSVAEA IKETGADVSI AFVPPAFSKD AIIEAIDAEI PLLVVITEGI PVQDSAYAWA YNVEKGNKTR IIGPNCPGII TPGEALVGIT PNNITGTGPV GLVSKSGTLT YQMMYELRDF GFSTAIGIGG DPVIGTTHID AIEAFEKDPE TKIIVMIGEI GGDAEERAAD YIKANVSKPV VGYVAGFTAP EGKXMGHAGA IVSGSSGTAX AKXEALEAAG VXVGKTPSXX XKLAREILXX L
Validated NT Sequence
attttgttta ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtcta tctttctgaa caaggattcg aaggtcatcg tccagggcat caccggtggt gagggcacca agcacaccgc gctgatgctc aaggccggta ctcaggttgt cggcggcgtc aacgcccgca aggccggaac caccgtgtcg catgtggata agagcggtca aagtgtcgac ttacccgtgt tcggcagtgt cgccgaggcc atcaaggaga ccggggccga cgtgtcgatc gccttcgtgc cgccggcctt ctccaaggac gccatcatcg aggcaatcga cgccgagatc ccgctgctcg ttgtcatcac tgagggaatc ccggtgcagg acagcgcgta tgcgtgggcc tacaacgtcg agaagggcaa caagacccgc atcatcgggc ccaactgccc cggcatcatc acccccggcg aggcgctggt gggtatcact cctaacaaca tcaccggcac cggtccggtc ggtctggtgt ccaagtccgg caccctgacc taccagatga tgtacgagct gcgcgatttc ggcttctcca ccgccatcgg tatcggcggc gaccccgtca tcggcaccac ccacatcgac gccatcgagg cgttcgagaa ggacccggag accaagatca tcgtcatgat cggtgagatc ggtggcgacg ccgaggagcg tgcggccgac tacatcaagg ccaacgtgtc caagcccgtc gtcggctacg ttgcgggctt caccgccccc gagggcaaga ncatgggcca cgccggtgcc atcgtgtccg gcagctcggg taccgcccan gccaagaang aggccctcga ggccgccggt gtgaangtcg gcaagacgcc gtccganang nncaagctgg cgcgagagat cctgcnnanc ctgtaatann agcacnaana ntnctgcagn cagcttcncn aatccnnngc tnncaagccc nanggnnnct gantnngctn ctgcc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSIFLNKDS KVIVQGITGG EGTKHTALML KAGTQVVGGV NARKAGTTVS HVDKSGQSVD LPVFGSVAEA IKETGADVSI AFVPPAFSKD AIIEAIDAEI PLLVVITEGI PVQDSAYAWA YNVEKGNKTR IIGPNCPGII TPGEALVGIT PNNITGTGPV GLVSKSGTLT YQMMYELRDF GFSTAIGIGG DPVIGTTHID AIEAFEKDPE TKIIVMIGEI GGDAEERAAD YIKANVSKPV VGYVAGFTAP EGKTMGHAGA IVSGSSGTAQ AKKEALEAAG VKVGKTPSET AKLAREILQS L
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctatcttt ctgaacaagg attcgaaggt catcgtccag ggcatcaccg gtggtgaggg caccaagcac accgcgctga tgctcaaggc cggtactcag gttgtcggcg gcgtcaacgc ccgcaaggcc ggaaccaccg tgtcgcatgt ggataagagc ggtcaaagtg tcgacttacc cgtgttcggc agtgtcgccg aggccatcaa ggagaccggg gccgacgtgt cgatcgcctt cgtgccgccg gccttctcca aggacgccat catcgaggca atcgacgccg agatcccgct gctcgttgtc atcactgagg gaatcccggt gcaggacagc gcgtatgcgt gggcctacaa cgtcgagaag ggcaacaaga cccgcatcat cgggcccaac tgccccggca tcatcacccc cggcgaggcg ctggtgggta tcactcctaa caacatcacc ggcaccggtc cggtcggtct ggtgtccaag tccggcaccc tgacctacca gatgatgtac gagctgcgcg atttcggctt ctccaccgcc atcggtatcg gcggcgaccc cgtcatcggc accacccaca tcgacgccat cgaggcgttc gagaaggacc cggagaccaa gatcatcgtc atgatcggtg agatcggtgg cgacgccgag gagcgtgcgg ccgactacat caaggccaac gtgtccaagc ccgtcgtcgg ctacgttgcg ggcttcaccg cccccgaggg caagaccatg ggccacgccg gtgccatcgt gtccggcagc tcgggtaccg cccaggccaa gaaggaggcc ctcgaggccg ccggtgtgaa ggtcggcaag acgccgtccg agacggccaa gctggcgcga gagatcctgc agagcctgta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a