MyabA.00549.a

Putative molybdenum cofactor biosynthesis protein A2

CENTER ID: MyabA.00549.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00549.a.A1.GE30313 Full length( MyabA.00549.a ) 1 330
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.908
RefSeq: YP_001701619.1
UniProt: B1MJ09

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTPLIDSFGR IATDLRVSLT DRCNLRCTYC MPAEGLDWLR SETLLTTDEY IRLIGIAVTR LGIDSVRFTG GEPLIFKDLE KLVAAVAGMS PRPDIAVTTN GVGLDRKAVA LREAGLDRIN VSLDTLDAHR FAAITRRDRL SDVLLGLKAA GSAGLSPIKI NAVLTPEQYK EDAVALLHFS LEHGYELRII EQMPLDAGHS WSRARMVTAD EIHAALSREF SLSEDPAPRG SAPAQRWLVN GGPATVGIIA SVSRPFCGDC DRTRLTADGQ VRNCLFATTE TDLRGLLRSG ASDAEVASAW QAAMWGKKPG HGINDPSFLQ PDRPMSAIGG
NT Sequence
ATGACCCCGT TAATTGATTC GTTCGGCCGC ATCGCGACCG ACTTACGGGT GTCGTTGACC GACCGGTGCA ATCTGCGCTG CACCTACTGT ATGCCCGCCG AAGGACTGGA CTGGCTGCGC AGCGAGACAC TGCTCACCAC CGATGAATAC ATCAGACTCA TCGGGATCGC CGTGACACGC CTCGGGATTG ACAGCGTTCG ATTCACCGGT GGTGAGCCGC TGATATTCAA GGACCTGGAG AAGCTCGTTG CCGCCGTCGC CGGCATGTCG CCGCGCCCGG ACATTGCCGT CACCACCAAC GGCGTCGGAC TCGACCGTAA GGCGGTTGCG CTGCGAGAGG CCGGATTGGA CCGCATCAAC GTCTCGCTGG ATACCTTGGA CGCCCATCGT TTTGCCGCGA TCACCCGGCG GGATCGGCTG TCCGACGTGC TGCTGGGCCT GAAGGCGGCA GGATCTGCCG GGCTGTCGCC GATAAAGATC AACGCGGTGC TGACACCCGA GCAGTACAAA GAGGATGCCG TCGCCCTGCT GCATTTCAGC CTGGAGCACG GGTACGAACT GCGGATCATC GAGCAGATGC CCCTGGACGC CGGACACAGC TGGAGCCGGG CCCGCATGGT GACGGCAGAC GAGATCCATG CCGCACTCAG CCGGGAGTTC TCCCTGAGCG AGGATCCCGC GCCCCGCGGC AGCGCCCCAG CGCAACGCTG GCTGGTGAAC GGGGGCCCGG CGACGGTCGG AATCATCGCG TCGGTGTCCC GGCCGTTCTG TGGCGACTGC GACCGCACCC GGTTGACCGC GGACGGGCAG GTGCGCAATT GCCTGTTCGC CACCACCGAG ACGGATCTGC GCGGCCTGCT CCGCTCGGGC GCGTCCGACG CCGAGGTGGC ATCGGCCTGG CAGGCCGCCA TGTGGGGCAA GAAGCCGGGG CACGGCATCA ACGACCCGTC CTTCCTGCAG CCGGACCGCC CGATGAGCGC GATCGGCGGT
Details for MyabA.00549.a.A1.GE30313
HARVESTED ON: 9/8/2010
SEQUENCED ON: 9/17/2010
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 91
PERCENT COVERAGE: 111
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTPLIDSFG RIATDLXVSL TDRCNLRCTY CMPAEGLDWL RSETLLTTDE YIRLIGIAVT RLGIDSVRFT GGEPLIFKDL EKLVAAVAGM SPRPDIAVTT NGVGLDRKAV ALREAGLDRI NVSLDTLDAH RFAAITRRDR LSDVLLGLKA AGSAGLSPIK INAVLTPEQY KEDAVALLHF SLEHGYELRI IEQMPLDAGH SWSRARMVTA DEIHAALSRE FSLSEDPAPR GSAPAQRWLV NGGPATVGII ASVSRPFCGD CDRTRLXADG XVRNCLFATT ETDLRGLLRS GASXXXVASX WQAAMWGRSX GTASXPVXXA XXXXDXRDRR XKQHXXXAXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX
Validated NT Sequence
ttttgtttac tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgacccc gttaattgat tcgttcggcc gcatcgcgac cgacttacng gtgtcgttga ccgaccggtg caatctgcgc tgcacctact gtatgcccgc cgaaggactg gactggctgc gcagcgagac actgctcacc accgatgaat acatcagact catcgggatc gccgtgacac gcctcgggat tgacagcgtt cgattcaccg gtggtgagcc gctgatattc aaggacctgg agaagctcgt tgccgccgtc gccggcatgt cgccgcgccc ggacattgcc gtcaccacca acggcgtcgg actcgaccgt aaggcggttg cgctgcgaga ggccggattg gaccgcatca acgtctcgct ggataccttg gacgcccatc gttttgccgc gatcacccgg cgggatcggc tgtccgacgt gctgctgggc ctgaaggcgg caggatctgc cgggctgtcg ccgataaaga tcaacgcggt gctgacaccc gagcagtaca aagaggatgc cgtcgccctg ctgcatttca gcctggagca cgggtacgaa ctgcggatca tcgagcagat gcccctggac gccggacaca gctggagccg ggcccgcatg gtgacggcag acgagatcca tgccgcactc agccgggagt tctccctgag cgaggatccc gcgccccgcg gcagcgcccc agcgcaacgc tggctggtga acgggggccc ggcgacngtc ggaatcatcg cgtcngtgtc ccggccgttc tgtggcgact gcgaccgcac ccggttganc gcggacgggc angtgcgcaa ttgcctgttc gccaccaccg agacggatct gcgcggcctg ctccgctcgg gcgcgtccga nnccgangtg gcatcnncct ggcaggccgc catgtggggc agaagcnggg gcacggcntc acnacccgtn nttnnngcan nnnnannnnc cgatnancgn gatcggcggn ntaaacagca cnannntncn gcannnnann nnnnnnngnn nnnnnnctnc aannnnnnna ancnnnnnnn nnncnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncnn nnnnnnnnnn na
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTPLIDSFG RIATDLRVSL TDRCNLRCTY CMPAEGLDWL RSETLLTTDE YIRLIGIAVT RLGIDSVRFT GGEPLIFKDL EKLVAAVAGM SPRPDIAVTT NGVGLDRKAV ALREAGLDRI NVSLDTLDAH RFAAITRRDR LSDVLLGLKA AGSAGLSPIK INAVLTPEQY KEDAVALLHF SLEHGYELRI IEQMPLDAGH SWSRARMVTA DEIHAALSRE FSLSEDPAPR GSAPAQRWLV NGGPATVGII ASVSRPFCGD CDRTRLTADG QVRNCLFATT ETDLRGLLRS GASDAEVASA WQAAMWGKKP GHGINDPSFL QPDRPMSAIG G
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccccgtta attgattcgt tcggccgcat cgcgaccgac ttacgggtgt cgttgaccga ccggtgcaat ctgcgctgca cctactgtat gcccgccgaa ggactggact ggctgcgcag cgagacactg ctcaccaccg atgaatacat cagactcatc gggatcgccg tgacacgcct cgggattgac agcgttcgat tcaccggtgg tgagccgctg atattcaagg acctggagaa gctcgttgcc gccgtcgccg gcatgtcgcc gcgcccggac attgccgtca ccaccaacgg cgtcggactc gaccgtaagg cggttgcgct gcgagaggcc ggattggacc gcatcaacgt ctcgctggat accttggacg cccatcgttt tgccgcgatc acccggcggg atcggctgtc cgacgtgctg ctgggcctga aggcggcagg atctgccggg ctgtcgccga taaagatcaa cgcggtgctg acacccgagc agtacaaaga ggatgccgtc gccctgctgc atttcagcct ggagcacggg tacgaactgc ggatcatcga gcagatgccc ctggacgccg gacacagctg gagccgggcc cgcatggtga cggcagacga gatccatgcc gcactcagcc gggagttctc cctgagcgag gatcccgcgc cccgcggcag cgccccagcg caacgctggc tggtgaacgg gggcccggcg acggtcggaa tcatcgcgtc ggtgtcccgg ccgttctgtg gcgactgcga ccgcacccgg ttgaccgcgg acgggcaggt gcgcaattgc ctgttcgcca ccaccgagac ggatctgcgc ggcctgctcc gctcgggcgc gtccgacgcc gaggtggcat cggcctggca ggccgccatg tggggcaaga agccggggca cggcatcaac gacccgtcct tcctgcagcc ggaccgcccg atgagcgcga tcggcggtta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a