MyabA.00504.a

Probable dehydrogenase/dehydratase (MaoC family)

CENTER ID:	MyabA.00504.a
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.00504.a.A1.GE30311	Full length( MyabA.00504.a )		1	285

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.00504.a.A1.PS00910	Full length( MyabA.00504.a )		1	285

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_129142
PATRIC ID:	fig\|36809.5.peg.660
RefSeq:	YP_001701373.1
UniProt:	B1MH35

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPINVDIALG AATEPAEFSW TASDVQLYHL AIGAGADPVS ETELRYLQDK TPQVLPTFAT VASGFHAVEP PKVSFPGIEI DLAKILHGTE QVTAHRPLPP SGTARSEGKV VEIWDKGKAA VIVTETTTSD DQGPLWTIRR SIFARGEGGF GGERGPSSHG GVPERDADLE VSTHILPQQA LLYRLCGDRN PLHSDPAFAA AAGFPRPILH GLCSYGVVCK AAVDAALDGD VSRVTSYAAK FAGVVFPGET LKTRIWKEDG KLLISAVVAD REDAPALADV ELTHS

NT Sequence

ATGCCTATCA ATGTCGATAT CGCGCTGGGC GCGGCTACCG AACCGGCCGA ATTTTCGTGG ACTGCGTCCG ATGTGCAGCT GTATCACCTG GCGATCGGTG CCGGTGCCGA TCCGGTGAGC GAGACCGAGC TGCGCTACCT GCAGGACAAG ACCCCGCAGG TACTGCCCAC CTTCGCGACC GTCGCCTCGG GCTTCCACGC GGTGGAGCCG CCCAAGGTGT CCTTTCCGGG GATCGAGATC GACCTGGCCA AGATCCTGCA CGGCACCGAA CAGGTGACTG CGCACCGGCC GCTCCCACCG TCAGGCACCG CCCGCTCCGA GGGCAAGGTC GTGGAAATCT GGGATAAGGG CAAGGCTGCC GTCATCGTCA CGGAGACGAC GACATCCGAT GATCAGGGCC CCCTCTGGAC CATCCGTCGT TCGATCTTCG CCCGCGGTGA AGGTGGCTTC GGCGGCGAGC GCGGTCCTTC TTCCCACGGC GGCGTTCCCG AACGCGACGC CGACCTCGAG GTGTCCACGC ACATCCTGCC GCAGCAGGCG CTGCTGTATC GGCTGTGTGG TGACCGTAAT CCGTTGCACT CCGATCCGGC CTTCGCGGCA GCGGCCGGGT TCCCCCGCCC CATCCTGCAT GGGCTGTGCA GCTACGGCGT GGTGTGCAAG GCAGCGGTCG ACGCGGCGCT GGACGGGGAC GTCTCGCGCG TGACGTCGTA TGCCGCGAAG TTCGCCGGAG TGGTGTTTCC AGGCGAGACC CTGAAGACCC GCATCTGGAA GGAAGACGGC AAGCTGCTGA TCTCCGCGGT GGTTGCTGAC CGGGAAGATG CCCCCGCGCT CGCCGATGTG GAGCTCACCC ACTCC

Details for MyabA.00504.a.A1.GE30311

HARVESTED ON:	9/8/2010
SEQUENCED ON:	9/17/2010
EXPECTED MW:	32kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	96

Validated AA Sequence

MGTLEAQTQG PGSMPINVDI ALGAATEPAE FSWTASDVQL YHLAIGAGAD PVSETELRYL QDKTPQVLPT FATVASGFHA VEPPKVSFPG IEIDLAKILH GTEQVTAHRP LPPSGTARSE GKVVEIWDKG KAAVIVTETT TSDDQGPLWT IRRSIFARGE GGFGGERGPS SHGGVPERDA DLEVSTHILP QQALLYRLCG DRNPLHSDPA FAAAAGFPRP ILHGLCSYGV VCKAAVDAAL DGDVSRVTSY AAKFAGVVFP GETLKTRIWK EDGKLLISAV VADREDAPAL ADVEL

Validated NT Sequence

gtgagctcca catcggcgag cgcgggggca tcttcccggt cagcaaccac cgcggagatc agcagcttgc cgtcttcctt ccagatgcgg gtcttcaggg tctcgcctgg aaacaccact ccggcgaact tcgcggcata cgacgtcacg cgcgagacgt ccccgtccag cgccgcgtcg accgctgcct tgcacaccac gccgtagctg cacagcccat gcaggatggg gcgggggaac ccggccgctg ccgcgaaggc cggatcggag tgcaacggat tacggtcacc acacagccga tacagcagcg cctgctgcgg caggatgtgc gtggacacct cgaggtcggc gtcgcgttcg ggaacgccgc cgtgggaaga aggaccgcgc tcgccgccga agccaccttc accgcgggcg aagatcgaac gacggatggt ccagaggggg ccctgatcat cggatgtcgt cgtctccgtg acgatgacgg cagccttgcc cttatcccag atttccacga ccttgccctc ggagcgggcg gtgcctgacg gtgggagcgg ccggtgcgca gtcacctgtt cggtgccgtg caggatcttg gccaggtcga tctcgatccc cggaaaggac accttgggcg gctccaccgc gtggaagccc gaggcgacgg tcgcgaaggt gggcagtacc tgcggggtct tgtcctgcag gtagcgcagc tcggtctcgc tcaccggatc ggcaccggca ccgatcgcca ggtgatacag ctgcacatcg gacgcagtcc acgaaaattc ggccggttcg gtagccgcgc ccagcgcgat atcgacattg ataggcatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMPINVDIAL GAATEPAEFS WTASDVQLYH LAIGAGADPV SETELRYLQD KTPQVLPTFA TVASGFHAVE PPKVSFPGIE IDLAKILHGT EQVTAHRPLP PSGTARSEGK VVEIWDKGKA AVIVTETTTS DDQGPLWTIR RSIFARGEGG FGGERGPSSH GGVPERDADL EVSTHILPQQ ALLYRLCGDR NPLHSDPAFA AAAGFPRPIL HGLCSYGVVC KAAVDAALDG DVSRVTSYAA KFAGVVFPGE TLKTRIWKED GKLLISAVVA DREDAPALAD VELTHS

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcctatcaat gtcgatatcg cgctgggcgc ggctaccgaa ccggccgaat tttcgtggac tgcgtccgat gtgcagctgt atcacctggc gatcggtgcc ggtgccgatc cggtgagcga gaccgagctg cgctacctgc aggacaagac cccgcaggta ctgcccacct tcgcgaccgt cgcctcgggc ttccacgcgg tggagccgcc caaggtgtcc tttccgggga tcgagatcga cctggccaag atcctgcacg gcaccgaaca ggtgactgcg caccggccgc tcccaccgtc aggcaccgcc cgctccgagg gcaaggtcgt ggaaatctgg gataagggca aggctgccgt catcgtcacg gagacgacga catccgatga tcagggcccc ctctggacca tccgtcgttc gatcttcgcc cgcggtgaag gtggcttcgg cggcgagcgc ggtccttctt cccacggcgg cgttcccgaa cgcgacgccg acctcgaggt gtccacgcac atcctgccgc agcaggcgct gctgtatcgg ctgtgtggtg accgtaatcc gttgcactcc gatccggcct tcgcggcagc ggccgggttc ccccgcccca tcctgcatgg gctgtgcagc tacggcgtgg tgtgcaaggc agcggtcgac gcggcgctgg acggggacgt ctcgcgcgtg acgtcgtatg ccgcgaagtt cgccggagtg gtgtttccag gcgagaccct gaagacccgc atctggaagg aagacggcaa gctgctgatc tccgcggtgg ttgctgaccg ggaagatgcc cccgcgctcg ccgatgtgga gctcacccac tcctaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa

Details for MyabA.00504.a.A1.PS00910

PURIFICATION DATe:	12/20/2010
CONCENTRATION:	31.62mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	1
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	96

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCTATCAAT GTCGATATCG CGCTGGGCGC GGCTACCGAA CCGGCCGAAT TTTCGTGGAC TGCGTCCGAT GTGCAGCTGT ATCACCTGGC GATCGGTGCC GGTGCCGATC CGGTGAGCGA GACCGAGCTG CGCTACCTGC AGGACAAGAC CCCGCAGGTA CTGCCCACCT TCGCGACCGT CGCCTCGGGC TTCCACGCGG TGGAGCCGCC CAAGGTGTCC TTTCCGGGGA TCGAGATCGA CCTGGCCAAG ATCCTGCACG GCACCGAACA GGTGACTGCG CACCGGCCGC TCCCACCGTC AGGCACCGCC CGCTCCGAGG GCAAGGTCGT GGAAATCTGG GATAAGGGCA AGGCTGCCGT CATCGTCACG GAGACGACGA CATCCGATGA TCAGGGCCCC CTCTGGACCA TCCGTCGTTC GATCTTCGCC CGCGGTGAAG GTGGCTTCGG CGGCGAGCGC GGTCCTTCTT CCCACGGCGG CGTTCCCGAA CGCGACGCCG ACCTCGAGGT GTCCACGCAC ATCCTGCCGC AGCAGGCGCT GCTGTATCGG CTGTGTGGTG ACCGTAATCC GTTGCACTCC GATCCGGCCT TCGCGGCAGC GGCCGGGTTC CCCCGCCCCA TCCTGCATGG GCTGTGCAGC TACGGCGTGG TGTGCAAGGC AGCGGTCGAC GCGGCGCTGG ACGGGGACGT CTCGCGCGTG ACGTCGTATG CCGCGAAGTT CGCCGGAGTG GTGTTTCCAG GCGAGACCCT GAAGACCCGC ATCTGGAAGG AAGACGGCAA GCTGCTGATC TCCGCGGTGG TTGCTGACCG GGAAGATGCC CCCGCGCTCG CCGATGTGGA GCTCACCCAC TCCTAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA