MyabA.00471.d

Hypothetical fumarylacetoacetate hydrolase family

CENTER ID: MyabA.00471.d
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00471.d.A1.GE30310 Full length( MyabA.00471.d ) 1 288
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.4438
RefSeq: YP_001705086.1
UniProt: B1MJS5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRLYTTDRGI GREDRHGILA LLDLPHRDLG ELLSDTGLDP ARDATVTDEV ALTDVAVLAP VIRPGKILII GLNYPSHAKE ALAAFAAMGR DDIVMPTEPN MQLIAGSATT GTDHTIKLPA IAPDEVDYEG ELAVVIGRST HAVSVEEAWK HVAGLTIAND VGARDIQRRA MTGDAAASIG VAKSFDTFKP LGPCLVTADE FSDGVDLTLQ TYVNGELRQD DRTGSFIYPI PELIAHLSRY QTLQPGDVIC TGTPCGAGAF TGRFLRSGDT VEVAIERIGL LRNRVSAA
NT Sequence
ATGCGCCTGT ATACAACTGA TCGAGGGATC GGCCGCGAAG ATCGCCATGG CATCCTCGCG CTGCTCGATC TTCCCCACCG TGACCTGGGC GAACTGCTAT CGGACACCGG CTTGGATCCA GCCCGCGACG CGACCGTCAC CGACGAGGTC GCACTGACGG ACGTTGCGGT ACTGGCGCCG GTGATCCGCC CGGGAAAGAT CCTCATCATC GGCCTGAATT ACCCAAGTCA TGCGAAGGAA GCGCTTGCGG CCTTCGCAGC CATGGGACGC GATGACATCG TCATGCCGAC AGAACCCAAT ATGCAACTGA TCGCGGGGTC GGCCACCACG GGTACCGATC ACACCATCAA ACTGCCTGCG ATCGCTCCCG ACGAGGTGGA CTATGAGGGT GAACTGGCCG TTGTGATCGG CCGCAGCACC CATGCCGTCT CGGTGGAAGA GGCATGGAAG CACGTGGCCG GACTGACAAT CGCCAACGAC GTCGGAGCGC GCGATATTCA ACGTCGCGCA ATGACGGGTG ATGCCGCCGC GTCGATCGGG GTGGCCAAGA GCTTCGACAC GTTCAAGCCC CTCGGACCAT GCTTGGTCAC GGCGGACGAG TTCTCCGACG GGGTAGACCT CACACTCCAG ACGTATGTGA ATGGTGAACT ACGACAAGAT GATCGGACGG GTAGCTTCAT ATACCCCATC CCGGAGTTGA TCGCACATCT GTCGCGGTAT CAGACCCTGC AACCCGGCGA CGTGATCTGC ACCGGCACGC CATGCGGCGC CGGCGCATTC ACTGGCCGCT TTCTGCGGTC CGGCGATACC GTCGAGGTCG CAATCGAACG TATCGGGCTA CTGCGCAACA GGGTATCTGC CGCA
Details for MyabA.00471.d.A1.GE30310
HARVESTED ON: 9/8/2010
SEQUENCED ON: 9/17/2010
EXPECTED MW: 32kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 97
PERCENT COVERAGE: 109
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRLYTTDRG IGREDRHGIL ALLDLPHRDL GELLSDTGLD PARDATVTDE VALTDVAVLA PVIRPGKILI IGLNYPSHAK EALAAFAAMG RDDIVMPTEP NMQLIAGSAT TGTDHTIKLP AIAPDEVDYE GELAVVIGRS THAVSVEEAW KHVAGLTIAN DVGARDIQRR AMTGDAAASI GVAKSFDTFK PLGPCLVTAD EFSDGVDLTL QTYVNGELRQ DDRTGSFIYP IPELIAHLSR YQXLQPGDVI CTGTPCGAXA FTGRFLRSGD TVEVAIERIG LLRNXXLXXN KQHEQVLQPS FSRIXLLTKP EXKLXGCC
Validated NT Sequence
ttttgtttac tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgcgcct gtatacaact gatcgaggga tcggccgcga agatcgccat ggcatcctcg cgctgctcga tcttccccac cgtgacctgg gcgaactgct atcggacacc ggcttggatc cagcccgcga cgcgaccgtc accgacgagg tcgcactgac ggacgttgcg gtactggcgc cggtgatccg cccgggaaag atcctcatca tcggcctgaa ttacccaagt catgcgaagg aagcgcttgc ggccttcgca gccatgggac gcgatgacat cgtcatgccg acagaaccca atatgcaact gatcgcgggg tcggccacca cgggtaccga tcacaccatc aaactgcctg cgatcgctcc cgacgaggtg gactatgagg gtgaactggc cgttgtgatc ggccgcagca cccatgccgt ctcggtggaa gaggcatgga agcacgtggc cggactgaca atcgccaacg acgtcggagc gcgcgatatt caacgtcgcg caatgacggg tgatgccgcc gcgtcgatcg gggtggccaa gagcttcgac acgttcaagc ccctcggacc atgcttggtc acggcggacg agttctccga cggggtagac ctcacactcc agacgtatgt gaatggtgaa ctacgacaag atgatcggac gggtagcttc atatacccca tcccggagtt gatcgcacat ctgtcgcggt atcagancct gcaacccggc gacgtgatct gcaccggcac gccatgcggc gccngcgcat tcactggccg ctttctgcgg tccggcgata ccgtcgaggt cgcaatcgaa cgtatcgggc tactgcgcaa cnnnnatctg cnnnataata aacagcacga acaagttctg cagccaagct tctcgaggat ccnnctgcta acaaagcccg aaangaagct anttggctgc tgcnnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRLYTTDRG IGREDRHGIL ALLDLPHRDL GELLSDTGLD PARDATVTDE VALTDVAVLA PVIRPGKILI IGLNYPSHAK EALAAFAAMG RDDIVMPTEP NMQLIAGSAT TGTDHTIKLP AIAPDEVDYE GELAVVIGRS THAVSVEEAW KHVAGLTIAN DVGARDIQRR AMTGDAAASI GVAKSFDTFK PLGPCLVTAD EFSDGVDLTL QTYVNGELRQ DDRTGSFIYP IPELIAHLSR YQTLQPGDVI CTGTPCGAGA FTGRFLRSGD TVEVAIERIG LLRNRVSAA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgcctgtat acaactgatc gagggatcgg ccgcgaagat cgccatggca tcctcgcgct gctcgatctt ccccaccgtg acctgggcga actgctatcg gacaccggct tggatccagc ccgcgacgcg accgtcaccg acgaggtcgc actgacggac gttgcggtac tggcgccggt gatccgcccg ggaaagatcc tcatcatcgg cctgaattac ccaagtcatg cgaaggaagc gcttgcggcc ttcgcagcca tgggacgcga tgacatcgtc atgccgacag aacccaatat gcaactgatc gcggggtcgg ccaccacggg taccgatcac accatcaaac tgcctgcgat cgctcccgac gaggtggact atgagggtga actggccgtt gtgatcggcc gcagcaccca tgccgtctcg gtggaagagg catggaagca cgtggccgga ctgacaatcg ccaacgacgt cggagcgcgc gatattcaac gtcgcgcaat gacgggtgat gccgccgcgt cgatcggggt ggccaagagc ttcgacacgt tcaagcccct cggaccatgc ttggtcacgg cggacgagtt ctccgacggg gtagacctca cactccagac gtatgtgaat ggtgaactac gacaagatga tcggacgggt agcttcatat accccatccc ggagttgatc gcacatctgt cgcggtatca gaccctgcaa cccggcgacg tgatctgcac cggcacgcca tgcggcgccg gcgcattcac tggccgcttt ctgcggtccg gcgataccgt cgaggtcgca atcgaacgta tcgggctact gcgcaacagg gtatctgccg cataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa