MyabA.00471.b
Probable 2-hydroxyhepta-2,4-diene-1, 7-dioate isomerase
| CENTER ID: | MyabA.00471.b |
| ORGANISM: | Mycobacterium abscessus ATCC 19977 / DSM 44196 |
| ASSOCIATED DISEASE: | Skin abscess |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyabA.00471.b.A1.GE30308 | Full length( MyabA.00471.b ) | 1 | 308 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyabA.00471.b.A1.PS00908 | Full length( MyabA.00471.b ) | 1 | 308 |
Structures
3R6O
DEPOSITED: 3/22/2011
DETERMINATION: XRay
CLONE: MyabA.00471.b.A1.GE30308
PROTEIN: MyabA.00471.b.A1.PS00908
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126763 |
| PATRIC ID: | fig|36809.5.peg.952 |
| RefSeq: | YP_001701663.1 |
| UniProt: | B1MJ53 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNLRIVSFAG ADQWQPGVLI DDDTVAPLAP MLARHDIQVS SARELIALLP QISPLISDEL AGGSFEPVPL DSVALGAPIP EPGQVIALGF NYPTHSDTDS PLPKMADPVV FMKSPTSISG PRDAVIAPRT SHALDYEIEI AVVIGKPGYR IERSQAIKHV AGYMLANDIT ARDVALPFGF GGSPLQAQVV RGKGYPTFCP TGPWLFTTGS DTTFETFDFE LRINGELRQS GSTVDMTLGF AEVVETVSAT IALRAGDIIL TGTPGGCGFQ FDPPRYLRPG DVIEAHSAKL GKMRLPVHDE KPIESASR
NT Sequence
ATGAATTTGC GGATCGTCAG CTTCGCCGGT GCCGATCAAT GGCAACCCGG CGTGCTCATC GACGATGACA CCGTCGCTCC CCTTGCGCCG ATGCTCGCCC GTCACGACAT CCAGGTGTCC TCGGCACGCG AGCTGATAGC ACTGTTACCG CAGATCTCGC CGTTGATCTC TGACGAACTC GCTGGTGGCT CTTTCGAACC GGTGCCGCTC GACTCCGTTG CGTTGGGGGC CCCGATTCCC GAGCCCGGCC AGGTCATTGC TCTGGGCTTC AACTACCCGA CACATTCAGA CACCGATTCC CCGCTGCCGA AGATGGCGGA CCCCGTCGTC TTCATGAAGT CGCCCACCAG CATTAGCGGC CCCCGTGACG CCGTCATCGC GCCGCGGACA TCGCACGCAC TCGATTACGA AATCGAAATT GCTGTCGTCA TTGGGAAACC CGGATACCGC ATCGAGCGCA GTCAGGCGAT CAAGCACGTC GCCGGATATA TGCTCGCCAA CGACATCACT GCCCGCGATG TTGCGCTACC GTTCGGCTTC GGCGGTTCGC CATTGCAGGC GCAGGTTGTC CGCGGCAAGG GATACCCGAC ATTCTGCCCA ACTGGCCCGT GGCTCTTTAC CACCGGCTCA GATACGACGT TTGAAACGTT CGACTTCGAA CTTCGGATCA ACGGCGAACT GCGGCAGAGC GGTTCAACGG TAGACATGAC TCTCGGCTTC GCCGAGGTTG TTGAAACAGT GAGTGCAACC ATCGCCCTGC GCGCCGGGGA CATCATCCTG ACCGGAACGC CCGGCGGCTG CGGATTCCAA TTCGATCCTC CGCGCTACCT CCGACCGGGG GATGTGATCG AAGCTCATTC GGCCAAGTTG GGCAAAATGC GGCTACCTGT CCACGACGAA AAACCCATCG AGAGCGCATC GAGG
Details for MyabA.00471.b.A1.GE30308
| HARVESTED ON: | 9/8/2010 |
| SEQUENCED ON: | 9/17/2010 |
| EXPECTED MW: | 35kDa |
| OBSERVED MW: | 36kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 93 |
Validated AA Sequence
MNLRIVSFAG ADQWQPGVLI DDDTVAPLAP MLARHDIQVS SARELIALLP QISPLISDEL AGGSFEPVPL DSVALGAPIP EPGQVIALGF NYPTHSDTDS PLPKMADPVV FMKSPTSISG PRDAVIAPRT SHALDYEIEI AVVIGKPGYR IERSQAIKHV AGYMLANDIT ARDVALPFGF GGSPLQAQVV RGKGYPTFCP TGPWLFTTGS DTTFETFDFE LRINGELRQS GSTVDMTLGF AEVVETVSAT IALRAGDIIL TGTPGGCGFQ FDPPRYLRPG DVIEAHSAKL GKMRLPVHDE KPIESASR
Validated NT Sequence
ttattacctc gatgcgctct cgatgggttt ttcgtcgtgg acaggtagcc gcattttgcc caacttggcc gaatgagctt cgatcacatc ccccggtcgg aggtagcgcg gaggatcgaa ttggaatccg cagccgccgg gcgttccggt caggatgatg tccccggcgc gcagggcgat ggttgcactc actgtttcaa caacctcggc gaagccgaga gtcatgtcta ccgttgaacc gctctgccgc agttcgccgt tgatccgaag ttcgaagtcg aacgtttcaa acgtcgtatc tgagccggtg gtaaagagcc acgggccagt tgggcagaat gtcgggtatc ccttgccgcg gacaacctgc gcctgcaatg gcgaaccgcc gaagccgaac ggtagcgcaa catcgcgggc agtgatgtcg ttggcgagca tatatccggc gacgtgcttg atcgcctgac tgcgctcgat gcggtatccg ggtttcccaa tgacgacagc aatttcgatt tcgtaatcga gtgcgtgcga tgtccgcggc gcgatgacgg cgtcacgggg gccgctaatg ctggtgggcg acttcatgaa gacgacgggg tccgccatct tcggcagcgg ggaatcggtg tctgaatgtg tcgggtagtt gaagcccaga gcaatgacct ggccgggctc gggaatcggg gcccccaacg caacggagtc gagcggcacc ggttcgaaag agccaccagc gagttcgtca gagatcaacg gcgagatctg cggtaacagt gctatcagct cgcgtgccga ggacacctgg atgtcgtgac gggcgagcat cggcgcaagg ggagcgacgg tgtcatcgtc gatgagcacg ccgggttgcc attgatcggc accggcgaag ctgacgatcc gcaaattcat cgaaccagga ccctgggtct gagcttccag g
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNLRIVSFA GADQWQPGVL IDDDTVAPLA PMLARHDIQV SSARELIALL PQISPLISDE LAGGSFEPVP LDSVALGAPI PEPGQVIALG FNYPTHSDTD SPLPKMADPV VFMKSPTSIS GPRDAVIAPR TSHALDYEIE IAVVIGKPGY RIERSQAIKH VAGYMLANDI TARDVALPFG FGGSPLQAQV VRGKGYPTFC PTGPWLFTTG SDTTFETFDF ELRINGELRQ SGSTVDMTLG FAEVVETVSA TIALRAGDII LTGTPGGCGF QFDPPRYLRP GDVIEAHSAK LGKMRLPVHD EKPIESASR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaatttgcgg atcgtcagct tcgccggtgc cgatcaatgg caacccggcg tgctcatcga cgatgacacc gtcgctcccc ttgcgccgat gctcgcccgt cacgacatcc aggtgtcctc ggcacgcgag ctgatagcac tgttaccgca gatctcgccg ttgatctctg acgaactcgc tggtggctct ttcgaaccgg tgccgctcga ctccgttgcg ttgggggccc cgattcccga gcccggccag gtcattgctc tgggcttcaa ctacccgaca cattcagaca ccgattcccc gctgccgaag atggcggacc ccgtcgtctt catgaagtcg cccaccagca ttagcggccc ccgtgacgcc gtcatcgcgc cgcggacatc gcacgcactc gattacgaaa tcgaaattgc tgtcgtcatt gggaaacccg gataccgcat cgagcgcagt caggcgatca agcacgtcgc cggatatatg ctcgccaacg acatcactgc ccgcgatgtt gcgctaccgt tcggcttcgg cggttcgcca ttgcaggcgc aggttgtccg cggcaaggga tacccgacat tctgcccaac tggcccgtgg ctctttacca ccggctcaga tacgacgttt gaaacgttcg acttcgaact tcggatcaac ggcgaactgc ggcagagcgg ttcaacggta gacatgactc tcggcttcgc cgaggttgtt gaaacagtga gtgcaaccat cgccctgcgc gccggggaca tcatcctgac cggaacgccc ggcggctgcg gattccaatt cgatcctccg cgctacctcc gaccggggga tgtgatcgaa gctcattcgg ccaagttggg caaaatgcgg ctacctgtcc acgacgaaaa acccatcgag agcgcatcga ggtaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for MyabA.00471.b.A1.PS00908
| PURIFICATION DATe: | 12/20/2010 |
| CONCENTRATION: | 37.99mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 93 |
Protocol Notes
notes unavailable
Validated AA Sequence
MNLRIVSFAG ADQWQPGVLI DDDTVAPLAP MLARHDIQVS SARELIALLP QISPLISDEL AGGSFEPVPL DSVALGAPIP EPGQVIALGF NYPTHSDTDS PLPKMADPVV FMKSPTSISG PRDAVIAPRT SHALDYEIEI AVVIGKPGYR IERSQAIKHV AGYMLANDIT ARDVALPFGF GGSPLQAQVV RGKGYPTFCP TGPWLFTTGS DTTFETFDFE LRINGELRQS GSTVDMTLGF AEVVETVSAT IALRAGDIIL TGTPGGCGFQ FDPPRYLRPG DVIEAHSAKL GKMRLPVHDE KPIESASR
Validated NT Sequence
ttattacctc gatgcgctct cgatgggttt ttcgtcgtgg acaggtagcc gcattttgcc caacttggcc gaatgagctt cgatcacatc ccccggtcgg aggtagcgcg gaggatcgaa ttggaatccg cagccgccgg gcgttccggt caggatgatg tccccggcgc gcagggcgat ggttgcactc actgtttcaa caacctcggc gaagccgaga gtcatgtcta ccgttgaacc gctctgccgc agttcgccgt tgatccgaag ttcgaagtcg aacgtttcaa acgtcgtatc tgagccggtg gtaaagagcc acgggccagt tgggcagaat gtcgggtatc ccttgccgcg gacaacctgc gcctgcaatg gcgaaccgcc gaagccgaac ggtagcgcaa catcgcgggc agtgatgtcg ttggcgagca tatatccggc gacgtgcttg atcgcctgac tgcgctcgat gcggtatccg ggtttcccaa tgacgacagc aatttcgatt tcgtaatcga gtgcgtgcga tgtccgcggc gcgatgacgg cgtcacgggg gccgctaatg ctggtgggcg acttcatgaa gacgacgggg tccgccatct tcggcagcgg ggaatcggtg tctgaatgtg tcgggtagtt gaagcccaga gcaatgacct ggccgggctc gggaatcggg gcccccaacg caacggagtc gagcggcacc ggttcgaaag agccaccagc gagttcgtca gagatcaacg gcgagatctg cggtaacagt gctatcagct cgcgtgccga ggacacctgg atgtcgtgac gggcgagcat cggcgcaagg ggagcgacgg tgtcatcgtc gatgagcacg ccgggttgcc attgatcggc accggcgaag ctgacgatcc gcaaattcat cgaaccagga ccctgggtct gagcttccag g
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNLRIVSFA GADQWQPGVL IDDDTVAPLA PMLARHDIQV SSARELIALL PQISPLISDE LAGGSFEPVP LDSVALGAPI PEPGQVIALG FNYPTHSDTD SPLPKMADPV VFMKSPTSIS GPRDAVIAPR TSHALDYEIE IAVVIGKPGY RIERSQAIKH VAGYMLANDI TARDVALPFG FGGSPLQAQV VRGKGYPTFC PTGPWLFTTG SDTTFETFDF ELRINGELRQ SGSTVDMTLG FAEVVETVSA TIALRAGDII LTGTPGGCGF QFDPPRYLRP GDVIEAHSAK LGKMRLPVHD EKPIESASR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAATTTGCGG ATCGTCAGCT TCGCCGGTGC CGATCAATGG CAACCCGGCG TGCTCATCGA CGATGACACC GTCGCTCCCC TTGCGCCGAT GCTCGCCCGT CACGACATCC AGGTGTCCTC GGCACGCGAG CTGATAGCAC TGTTACCGCA GATCTCGCCG TTGATCTCTG ACGAACTCGC TGGTGGCTCT TTCGAACCGG TGCCGCTCGA CTCCGTTGCG TTGGGGGCCC CGATTCCCGA GCCCGGCCAG GTCATTGCTC TGGGCTTCAA CTACCCGACA CATTCAGACA CCGATTCCCC GCTGCCGAAG ATGGCGGACC CCGTCGTCTT CATGAAGTCG CCCACCAGCA TTAGCGGCCC CCGTGACGCC GTCATCGCGC CGCGGACATC GCACGCACTC GATTACGAAA TCGAAATTGC TGTCGTCATT GGGAAACCCG GATACCGCAT CGAGCGCAGT CAGGCGATCA AGCACGTCGC CGGATATATG CTCGCCAACG ACATCACTGC CCGCGATGTT GCGCTACCGT TCGGCTTCGG CGGTTCGCCA TTGCAGGCGC AGGTTGTCCG CGGCAAGGGA TACCCGACAT TCTGCCCAAC TGGCCCGTGG CTCTTTACCA CCGGCTCAGA TACGACGTTT GAAACGTTCG ACTTCGAACT TCGGATCAAC GGCGAACTGC GGCAGAGCGG TTCAACGGTA GACATGACTC TCGGCTTCGC CGAGGTTGTT GAAACAGTGA GTGCAACCAT CGCCCTGCGC GCCGGGGACA TCATCCTGAC CGGAACGCCC GGCGGCTGCG GATTCCAATT CGATCCTCCG CGCTACCTCC GACCGGGGGA TGTGATCGAA GCTCATTCGG CCAAGTTGGG CAAAATGCGG CTACCTGTCC ACGACGAAAA ACCCATCGAG AGCGCATCGA GGTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA