SSGCID: Target Page

MyabA.00416.a

Putative ABC transporter, ATP-binding protein

CENTER ID:	MyabA.00416.a
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.00416.a.A1.GE30300	Full length( MyabA.00416.a )		1	297

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.00416.a.A1.PS00915	Full length( MyabA.00416.a )		1	297

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|36809.5.peg.3447
RefSeq:	YP_001704114.1
UniProt:	B1MDZ1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSEMTRYRAP ADRSRGGIRH DDPVAEIDPD LLLDFRDVLL RRNRKVLVGP VTWSVELDER WVVLGPNGAG KTSLLRIAAA MEHPTSGYAA ILGERLGRVD VAELKSRIGL SSAALAQRIP DNEVVRDLVV SAGYAVLGRW REEYEEIDTA RAVDMLETLG AEHLSDRTYG TLSEGERKRV LIARALMTDP ELLLLDEPAA GLDLGGREEL VARLGELAAD PDAPAMVLVT HHVEEIPPGF SHALLLSEGG IVAQGLLADV MTSENLSRAF GQSIVVEMID GRYFARRARA RAAHRAQ

NT Sequence

ATGTCGGAGA TGACGCGCTA TAGGGCTCCC GCTGACCGCT CGCGCGGCGG GATACGGCAC GATGACCCAG TGGCCGAAAT TGATCCGGAC CTTCTGCTCG ATTTCCGCGA TGTGCTGCTG CGCCGCAACC GCAAGGTGTT GGTAGGCCCG GTCACCTGGT CGGTCGAGCT CGACGAACGC TGGGTGGTTC TCGGCCCCAA CGGCGCCGGG AAGACATCGT TGCTGCGAAT CGCGGCGGCC ATGGAGCACC CCACCTCTGG GTATGCGGCG ATTCTCGGCG AGCGGCTGGG CCGTGTCGAC GTCGCTGAAC TCAAGTCGAG GATCGGCCTG AGTTCGGCCG CGCTGGCGCA GCGCATTCCC GATAACGAGG TGGTTCGCGA TCTGGTGGTG TCGGCCGGAT ACGCGGTGCT GGGCCGCTGG CGCGAAGAAT ACGAAGAGAT CGATACCGCC CGTGCCGTGG ACATGCTCGA AACCCTTGGT GCCGAGCATC TGTCGGATCG TACGTACGGG ACGCTGTCCG AGGGGGAGCG CAAGCGGGTG CTCATCGCGC GGGCGTTGAT GACCGATCCG GAGCTGTTGC TCCTCGATGA ACCGGCCGCA GGCCTGGATC TGGGTGGGCG TGAGGAACTT GTCGCGCGGC TGGGTGAGCT TGCCGCCGAT CCGGACGCAC CCGCCATGGT TTTGGTGACT CATCATGTGG AGGAAATTCC GCCAGGGTTC AGTCACGCAT TGTTGCTGTC CGAGGGCGGG ATTGTCGCTC AGGGCCTGCT GGCCGATGTG ATGACCAGTG AGAATCTGTC CCGCGCGTTC GGCCAGTCGA TCGTGGTTGA GATGATCGAC GGGCGTTACT TCGCGCGACG TGCCCGCGCC AGGGCAGCCC ACCGTGCGCA G

Details for MyabA.00416.a.A1.GE30300

HARVESTED ON:	9/8/2010
SEQUENCED ON:	9/17/2010
EXPECTED MW:	34kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMSEMTRY RAPADRSRGG IRHDDPVAEI DPDLLLDFRD VLLRRNRKVL VGPVTWSVEL DERWVVLGPN GAGKTSLLRI AAAMEHPTSG YAAILGERLG RVDVAELKSR IGLSSAALAQ RIPDNEVVRD LVVSAGYAVL GRWREEYEEI DTARAVDMLE TLGAEHLSDR TYGTLSEGER KRVLIARALM TDPELLLLDE PAAGLDLGGR EELVARLGEL AADPDAPAMV LVTHHVEEIP PGFSHALLLS EGGIVAQGLL ADVMTSENLS RAFGQSIVVE MIDGRYFARR ARARAAHRAQ

Validated NT Sequence

tactgcgcac ggtgggctgc cctggcgcgg gcacgtcgcg cgaagtaacg cccgtcgatc atctcaacca cgatcgactg gccgaacgcg cgggacagat tctcactggt catcacatcg gccagcaggc cctgagcgac aatcccgccc tcggacagca acaatgcgtg actgaaccct ggcggaattt cctccacatg atgagtcacc aaaaccatgg cgggtgcgtc cggatcggcg gcaagctcac ccagccgcgc gacaagttcc tcacgcccac ccagatccag gcctgcggcc ggttcatcga ggagcaacag ctccggatcg gtcatcaacg cccgcgcgat gagcacccgc ttgcgctccc cctcggacag cgtcccgtac gtacgatccg acagatgctc ggcaccaagg gtttcgagca tgtccacggc acgggcggta tcgatctctt cgtattcttc gcgccagcgg cccagcaccg cgtatccggc cgacaccacc agatcgcgaa ccacctcgtt atcgggaatg cgctgcgcca gcgcggccga actcaggccg atcctcgact tgagttcagc gacgtcgaca cggcccagcc gctcgccgag aatcgccgca tacccagagg tggggtgctc catggccgcc gcgattcgca gcaacgatgt cttcccggcg ccgttggggc cgagaaccac ccagcgttcg tcgagctcga ccgaccaggt gaccgggcct accaacacct tgcggttgcg gcgcagcagc acatcgcgga aatcgagcag aaggtccgga tcaatttcgg ccactgggtc atcgtgccgt atcccgccgc gcgagcggtc agcgggagcc ctatagcgcg tcatctccga catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtga

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSEMTRYRA PADRSRGGIR HDDPVAEIDP DLLLDFRDVL LRRNRKVLVG PVTWSVELDE RWVVLGPNGA GKTSLLRIAA AMEHPTSGYA AILGERLGRV DVAELKSRIG LSSAALAQRI PDNEVVRDLV VSAGYAVLGR WREEYEEIDT ARAVDMLETL GAEHLSDRTY GTLSEGERKR VLIARALMTD PELLLLDEPA AGLDLGGREE LVARLGELAA DPDAPAMVLV THHVEEIPPG FSHALLLSEG GIVAQGLLAD VMTSENLSRA FGQSIVVEMI DGRYFARRAR ARAAHRAQ

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcggagatg acgcgctata gggctcccgc tgaccgctcg cgcggcggga tacggcacga tgacccagtg gccgaaattg atccggacct tctgctcgat ttccgcgatg tgctgctgcg ccgcaaccgc aaggtgttgg taggcccggt cacctggtcg gtcgagctcg acgaacgctg ggtggttctc ggccccaacg gcgccgggaa gacatcgttg ctgcgaatcg cggcggccat ggagcacccc acctctgggt atgcggcgat tctcggcgag cggctgggcc gtgtcgacgt cgctgaactc aagtcgagga tcggcctgag ttcggccgcg ctggcgcagc gcattcccga taacgaggtg gttcgcgatc tggtggtgtc ggccggatac gcggtgctgg gccgctggcg cgaagaatac gaagagatcg ataccgcccg tgccgtggac atgctcgaaa cccttggtgc cgagcatctg tcggatcgta cgtacgggac gctgtccgag ggggagcgca agcgggtgct catcgcgcgg gcgttgatga ccgatccgga gctgttgctc ctcgatgaac cggccgcagg cctggatctg ggtgggcgtg aggaacttgt cgcgcggctg ggtgagcttg ccgccgatcc ggacgcaccc gccatggttt tggtgactca tcatgtggag gaaattccgc cagggttcag tcacgcattg ttgctgtccg agggcgggat tgtcgctcag ggcctgctgg ccgatgtgat gaccagtgag aatctgtccc gcgcgttcgg ccagtcgatc gtggttgaga tgatcgacgg gcgttacttc gcgcgacgtg cccgcgccag ggcagcccac cgtgcgcagt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa

Details for MyabA.00416.a.A1.PS00915

PURIFICATION DATe:	12/22/2010
CONCENTRATION:	2.4mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	12
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCGGAGATG ACGCGCTATA GGGCTCCCGC TGACCGCTCG CGCGGCGGGA TACGGCACGA TGACCCAGTG GCCGAAATTG ATCCGGACCT TCTGCTCGAT TTCCGCGATG TGCTGCTGCG CCGCAACCGC AAGGTGTTGG TAGGCCCGGT CACCTGGTCG GTCGAGCTCG ACGAACGCTG GGTGGTTCTC GGCCCCAACG GCGCCGGGAA GACATCGTTG CTGCGAATCG CGGCGGCCAT GGAGCACCCC ACCTCTGGGT ATGCGGCGAT TCTCGGCGAG CGGCTGGGCC GTGTCGACGT CGCTGAACTC AAGTCGAGGA TCGGCCTGAG TTCGGCCGCG CTGGCGCAGC GCATTCCCGA TAACGAGGTG GTTCGCGATC TGGTGGTGTC GGCCGGATAC GCGGTGCTGG GCCGCTGGCG CGAAGAATAC GAAGAGATCG ATACCGCCCG TGCCGTGGAC ATGCTCGAAA CCCTTGGTGC CGAGCATCTG TCGGATCGTA CGTACGGGAC GCTGTCCGAG GGGGAGCGCA AGCGGGTGCT CATCGCGCGG GCGTTGATGA CCGATCCGGA GCTGTTGCTC CTCGATGAAC CGGCCGCAGG CCTGGATCTG GGTGGGCGTG AGGAACTTGT CGCGCGGCTG GGTGAGCTTG CCGCCGATCC GGACGCACCC GCCATGGTTT TGGTGACTCA TCATGTGGAG GAAATTCCGC CAGGGTTCAG TCACGCATTG TTGCTGTCCG AGGGCGGGAT TGTCGCTCAG GGCCTGCTGG CCGATGTGAT GACCAGTGAG AATCTGTCCC GCGCGTTCGG CCAGTCGATC GTGGTTGAGA TGATCGACGG GCGTTACTTC GCGCGACGTG CCCGCGCCAG GGCAGCCCAC CGTGCGCAGT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA