SSGCID: Target Page

MyabA.00404.a

Probable DNA-3-methyladenine glycosylase I TagA

CENTER ID:	MyabA.00404.a
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.00404.a.A1.GE30298	Full length( MyabA.00404.a )		1	192

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|36809.5.peg.1387
RefSeq:	YP_001702091.1
UniProt:	B1MLL0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPSKEPTRCA WAVDSAGSTL YTEYHDTEWG RPLYGRDALF ERLCLEAFQS GLAWITILRK REGFRRAFDG FRPEKVARYT DQDIARLMAD AEIVRNRAKI ESAISNARAV LDLDTDLSDL LWSFAPKRRA RPQLLSEVPA VTEESTAMAK ELKRRGFRFV GPTTAYALMQ ATGMVDDHVR SCWVPRAETA PI

NT Sequence

ATGCCGAGTA AGGAACCCAC CCGGTGTGCC TGGGCCGTGG ATTCGGCCGG ATCCACGTTG TACACCGAGT ACCACGACAC CGAATGGGGA CGGCCGCTGT ACGGCCGCGA TGCCTTGTTC GAGCGGCTAT GCCTGGAGGC CTTCCAGAGC GGCCTGGCCT GGATAACCAT CTTGCGCAAA CGCGAGGGCT TCCGGCGTGC GTTCGACGGT TTCCGACCCG AAAAAGTTGC CCGATACACC GACCAGGACA TTGCCCGGCT GATGGCGGAT GCCGAGATCG TGCGGAACCG CGCCAAGATC GAGTCGGCCA TCTCCAATGC CCGCGCCGTC CTCGATCTGG ACACCGATCT TTCGGACTTG CTGTGGTCGT TTGCGCCGAA ACGCCGCGCC CGGCCACAGC TTCTTTCGGA GGTGCCCGCG GTGACCGAAG AGAGCACCGC CATGGCCAAG GAACTCAAAC GTCGGGGGTT CCGTTTTGTG GGGCCTACCA CGGCATATGC ACTCATGCAG GCCACCGGAA TGGTGGACGA TCATGTGCGT TCGTGTTGGG TTCCGCGAGC CGAGACCGCA CCTATC

Details for MyabA.00404.a.A1.GE30298

HARVESTED ON:	9/8/2010
SEQUENCED ON:	9/17/2010
EXPECTED MW:	24kDa
OBSERVED MW:	24kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMPSKEPTRC AWAVDSAGST LYTEYHDTEW GRPLYGRDAL FERLCLEAFQ SGLAWITILR KREGFRRAFD GFRPEKVARY TDQDIARLMA DAEIVRNRAK IESAISNARA VLDLDTDLSD LLWSFAPKRR ARPQLLSEVP AVTEESTAMA KELKRRGFRF VGPTTAYALM QATGMVDDHV RSCWVPRAET API

Validated NT Sequence

tttgtttact ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgccgagt aaggaaccca cccggtgtgc ctgggccgtg gattcggccg gatccacgtt gtacaccgag taccacgaca ccgaatgggg acggccgctg tacggccgcg atgccttgtt cgagcggcta tgcctggagg ccttccagag cggcctggcc tggataacca tcttgcgcaa acgcgagggc ttccggcgtg cgttcgacgg tttccgaccc gaaaaagttg cccgatacac cgaccaggac attgcccggc tgatggcgga tgccgagatc gtgcggaacc gcgccaagat cgagtcggcc atctccaatg cccgcgccgt cctcgatctg gacaccgatc tttcggactt gctgtggtcg tttgcgccga aacgccgcgc ccggccacag cttctttcgg aggtgcccgc ggtgaccgaa gagagcaccg ccatggccaa ggaactcaaa cgtcgggggt tccgttttgt ggggcctacc acggcatatg cactcatgca ggccaccgga atggtggacg atcatgtgcg ttcgtgttgg gttccgcgag ccgagaccgc acctatctaa taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gcnnnnnnaa anaaa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccgagtaag gaacccaccc ggtgtgcctg ggccgtggat tcggccggat ccacgttgta caccgagtac cacgacaccg aatggggacg gccgctgtac ggccgcgatg ccttgttcga gcggctatgc ctggaggcct tccagagcgg cctggcctgg ataaccatct tgcgcaaacg cgagggcttc cggcgtgcgt tcgacggttt ccgacccgaa aaagttgccc gatacaccga ccaggacatt gcccggctga tggcggatgc cgagatcgtg cggaaccgcg ccaagatcga gtcggccatc tccaatgccc gcgccgtcct cgatctggac accgatcttt cggacttgct gtggtcgttt gcgccgaaac gccgcgcccg gccacagctt ctttcggagg tgcccgcggt gaccgaagag agcaccgcca tggccaagga actcaaacgt cgggggttcc gttttgtggg gcctaccacg gcatatgcac tcatgcaggc caccggaatg gtggacgatc atgtgcgttc gtgttgggtt ccgcgagccg agaccgcacc tatctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa