MyabA.00384.a

Putative cytoplasmic peptidase PepQ

CENTER ID: MyabA.00384.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00384.a.A1.GE30294 Full length( MyabA.00384.a ) 1 350
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.2885
RefSeq: YP_001703571.1
UniProt: B1MCE6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLRAEDLDAL LVTDLINIRY LTGFTGSAAA LWVAAGDGPE EKATVLCTDG RYRTQAAAQV PDLRALIDRR YGAALVAHAP AGRAGFESHV VTVDGFDKLA EAATDVELVR APNRIEKLRE VKDDSEVLLL ERACAAADTA LAALIETGGI RPGRTEREVA RDLENLMAEH GADGPSFETI VAAGPNSAIP HHRPTGAMLA AGDFVKIDFG ALVAGYHSDM TRTLVLGSAA PWQQEIHELV LTAQAAGRAA LAPGASLKNV DAAARDVITA AGYGENFGHG LGHGVGLQIH EAPGIGSTAT GTLLAGSAVT VEPGVYLPDR GGVRIEDTLV VRPDGPELLT RFPKELIVID
NT Sequence
GTGCTGCGCG CCGAGGATCT TGATGCGCTC CTGGTCACCG ATCTGATCAA TATTCGCTAC CTGACGGGTT TCACCGGCTC GGCCGCCGCG CTGTGGGTGG CGGCCGGGGA CGGGCCGGAG GAAAAGGCCA CCGTGCTGTG CACCGACGGC AGGTACCGGA CCCAGGCCGC CGCGCAGGTT CCTGACCTGC GCGCCCTGAT CGACCGCCGA TACGGTGCGG CTCTGGTGGC ACACGCCCCT GCCGGACGCG CGGGCTTCGA AAGTCACGTC GTGACCGTCG ACGGATTCGA CAAATTGGCC GAAGCCGCAA CCGATGTCGA GTTGGTGCGG GCGCCGAACC GGATCGAGAA ACTGCGGGAA GTCAAGGACG ATTCCGAGGT CCTGCTTCTT GAAAGAGCTT GTGCGGCAGC AGATACCGCG CTGGCTGCGC TGATCGAGAC CGGAGGGATC CGCCCCGGGC GCACCGAACG TGAGGTGGCG CGGGACCTGG AAAACCTCAT GGCGGAGCAT GGCGCGGACG GCCCGTCCTT CGAGACCATC GTGGCCGCCG GGCCGAACTC GGCAATACCG CATCACCGGC CCACCGGCGC GATGCTGGCT GCCGGGGACT TCGTGAAGAT CGACTTCGGC GCGCTGGTCG CCGGATACCA CTCCGACATG ACCAGAACCC TGGTGCTGGG CTCGGCAGCG CCCTGGCAGC AGGAGATCCA TGAGCTGGTA CTGACCGCAC AGGCGGCCGG GCGCGCGGCG CTGGCGCCGG GGGCATCGCT GAAGAATGTC GACGCGGCCG CCCGCGATGT GATCACCGCC GCCGGATACG GAGAGAACTT CGGTCACGGC CTTGGTCATG GCGTGGGTTT GCAGATCCAC GAAGCACCGG GCATCGGATC GACAGCCACC GGTACACTGC TTGCTGGCTC GGCGGTGACA GTCGAGCCCG GGGTCTACCT GCCCGACCGG GGCGGAGTCC GGATCGAGGA CACGCTCGTG GTGCGGCCCG ACGGGCCGGA GCTGTTGACC CGGTTCCCCA AGGAACTCAT CGTTATCGAC
Details for MyabA.00384.a.A1.GE30294
HARVESTED ON: 9/8/2010
SEQUENCED ON: 9/17/2010
EXPECTED MW: 38kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 86
PERCENT COVERAGE: 105
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVLRAEDLD ALLVTDLINI RYLTGFTGSA AALWVAAGDG PEEKATVLCT DGRYRTQAAA QVPDLRALID RRYGAALVAH APAGRAGFES HVVTVDGFDK LAEAATDVEL VRAPNRIEKL REVKDDSEVL LLERACAAAD TALAALIETG GIRPGRTERE VARDLENLMA EHGADGPSFE TIVAAGPNSA IPHHRPTGAM LAAGDFVKID FGALVAGYHS DMTRTLVLGS AAPWQQEIHE LVLTAQAAGR AALAPGASLK NVDAAARDVI TAAGYXENFX HGLGHGVGLX IHEAPGIXSX ATGYTACWXX GDSXXXGXXX XXGXXXXSXT LXXARXXXXX XXPXXSXXTX NXXXXXXXXX XXXXXXXXXX X
Validated NT Sequence
ttttgtnnnn tttaagangg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggtgct gcgcgccgag gatcttgatg cgctcctggt caccgatctg atcaatattc gctacctgac gggtttcacc ggctcggccg ccgcgctgtg ggtggcggcc ggggacgggc cggaggaaaa ggccaccgtg ctgtgcaccg acggcaggta ccggacccag gccgccgcgc aggttcctga cctgcgcgcc ctgatcgacc gccgatacgg tgcggctctg gtggcacacg cccctgccgg acgcgcgggc ttcgaaagtc acgtcgtgac cgtcgacgga ttcgacaaat tggccgaagc cgcaaccgat gtcgagttgg tgcgggcgcc gaaccggatc gagaaactgc gggaagtcaa ggacgattcc gaggtcctgc ttcttgaaag agcttgtgcg gcagcagata ccgcgctggc tgcgctgatc gagaccggag ggatccgccc cgggcgcacc gaacgtgagg tggcgcggga cctggaaaac ctcatggcgg agcatggcgc ggacggcccg tccttcgaga ccatcgtggc cgccgggccg aactcggcaa taccgcatca ccggcccacc ggcgcgatgc tggctgccgg ggacttcgtg aagatcgact tcggcgcgct ggtcgccgga taccactccg acatgaccag aaccctggtg ctgggctcgg cagcgccctg gcagcaggag atccatgagc tggtactgac cgcacaggcg gccgggcgcg cggcgctggc gccgggggca tcgctgaaga atgtcgacgc ggccgcccgc gatgtgatca ccgccgccgg atacngagag aacttcngtc acggccttgg tcatggcgtg ggtttgcana tccacgaagc accgggcatc gnatcgncag ccaccgggta cactgcttgc tggntcngcg gtgacagtcn ancccngggn ntacnnncnn nancnggggn nnantncnna tcnnngacnc tcnnnnnngc ccgnnnnnnn nnncnnnnan nnnnnccnnn ntnatcntnn nngactnnta acannnnnnn nnnnnncanc nnnnnnnnna nnnnnannnn nnnnnnnnnn nnnnnnnnnn ncnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVLRAEDLD ALLVTDLINI RYLTGFTGSA AALWVAAGDG PEEKATVLCT DGRYRTQAAA QVPDLRALID RRYGAALVAH APAGRAGFES HVVTVDGFDK LAEAATDVEL VRAPNRIEKL REVKDDSEVL LLERACAAAD TALAALIETG GIRPGRTERE VARDLENLMA EHGADGPSFE TIVAAGPNSA IPHHRPTGAM LAAGDFVKID FGALVAGYHS DMTRTLVLGS AAPWQQEIHE LVLTAQAAGR AALAPGASLK NVDAAARDVI TAAGYGENFG HGLGHGVGLQ IHEAPGIGST ATGTLLAGSA VTVEPGVYLP DRGGVRIEDT LVVRPDGPEL LTRFPKELIV ID
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgctgcgc gccgaggatc ttgatgcgct cctggtcacc gatctgatca atattcgcta cctgacgggt ttcaccggct cggccgccgc gctgtgggtg gcggccgggg acgggccgga ggaaaaggcc accgtgctgt gcaccgacgg caggtaccgg acccaggccg ccgcgcaggt tcctgacctg cgcgccctga tcgaccgccg atacggtgcg gctctggtgg cacacgcccc tgccggacgc gcgggcttcg aaagtcacgt cgtgaccgtc gacggattcg acaaattggc cgaagccgca accgatgtcg agttggtgcg ggcgccgaac cggatcgaga aactgcggga agtcaaggac gattccgagg tcctgcttct tgaaagagct tgtgcggcag cagataccgc gctggctgcg ctgatcgaga ccggagggat ccgccccggg cgcaccgaac gtgaggtggc gcgggacctg gaaaacctca tggcggagca tggcgcggac ggcccgtcct tcgagaccat cgtggccgcc gggccgaact cggcaatacc gcatcaccgg cccaccggcg cgatgctggc tgccggggac ttcgtgaaga tcgacttcgg cgcgctggtc gccggatacc actccgacat gaccagaacc ctggtgctgg gctcggcagc gccctggcag caggagatcc atgagctggt actgaccgca caggcggccg ggcgcgcggc gctggcgccg ggggcatcgc tgaagaatgt cgacgcggcc gcccgcgatg tgatcaccgc cgccggatac ggagagaact tcggtcacgg ccttggtcat ggcgtgggtt tgcagatcca cgaagcaccg ggcatcggat cgacagccac cggtacactg cttgctggct cggcggtgac agtcgagccc ggggtctacc tgcccgaccg gggcggagtc cggatcgagg acacgctcgt ggtgcggccc gacgggccgg agctgttgac ccggttcccc aaggaactca tcgttatcga ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa