MyabA.00309.c

Probable acyl-CoA dehydrogenase FadE

CENTER ID: MyabA.00309.c
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00309.c.A1.GE30288 Full length( MyabA.00309.c ) 1 432
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.4993
RefSeq: YP_001705633.1
UniProt: B1MMJ4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTAMAWDFET DPEYQELLDW ADEFVRTEVE PLDYVFPNPY DKSDTVAMDY VRPLKEEVKR RGLWACHLGP ELGGPGYGQL KLALLNEILG RSVWAPSIFG CQAPDTGNAE ILAHYGTEAQ KAKYLQPLMD GDIGSCYVMT EPQGGSDPTL FKTTAVRDGD EWILNGEKWF NSEARHAAFW IVMAVTNTEV SPYQGMSMFI VDPNAPGVEI VRNITVHGFS EDEAYTRFTN ARIPAENMLG AEGGAFAIAQ TRLGGGRVHH AMRTVAQVRK AFDMMCERVV SREARGGTIA KLQMTQERIA DSWIEMESFR LLVLRTAWLI DKHQDYLKVR KDISAIKAAM PKVMHDVAQR ALHLHGSLGV SEEMPLAKMF LYSEVMGLVD GPTEVHKVTI AKEVLKDYTP YEGLFPPSHI PALTEKARAH VAARLEHRVG NL
NT Sequence
GTGACCGCCA TGGCATGGGA TTTCGAAACC GACCCGGAAT ACCAGGAACT GCTTGACTGG GCCGACGAGT TTGTCCGCAC CGAGGTCGAA CCCCTCGATT ACGTGTTCCC CAACCCATAC GACAAGTCCG ACACGGTGGC CATGGATTAC GTGCGGCCGC TCAAGGAAGA GGTCAAACGC CGCGGCCTGT GGGCATGTCA TCTGGGACCG GAGCTGGGTG GTCCGGGTTA CGGCCAGCTC AAGCTGGCGC TGCTCAACGA GATTCTGGGT AGGTCGGTGT GGGCTCCTTC GATCTTCGGA TGCCAGGCAC CCGATACCGG CAATGCCGAG ATCCTCGCGC ACTACGGCAC CGAAGCGCAG AAGGCCAAAT ACCTGCAGCC ACTGATGGAC GGCGACATCG GGTCCTGTTA TGTGATGACC GAGCCGCAGG GCGGATCGGA TCCGACGCTG TTCAAGACCA CCGCCGTCCG TGACGGTGAC GAATGGATCC TCAATGGTGA GAAGTGGTTC AACTCCGAGG CGCGGCACGC CGCGTTCTGG ATCGTCATGG CCGTCACCAA CACCGAAGTC AGTCCCTATC AGGGGATGTC GATGTTCATC GTCGACCCGA ACGCGCCCGG TGTCGAGATC GTCCGGAACA TCACGGTGCA CGGGTTCAGT GAAGACGAGG CCTACACCCG CTTCACCAAC GCCCGCATTC CTGCCGAGAA CATGCTGGGT GCCGAGGGGG GTGCGTTCGC CATCGCACAG ACGCGTCTCG GTGGTGGTCG TGTACATCAC GCCATGCGCA CGGTGGCCCA GGTACGCAAG GCCTTCGACA TGATGTGCGA ACGCGTCGTC AGCCGGGAGG CCCGAGGCGG CACCATCGCC AAACTCCAGA TGACCCAGGA ACGTATCGCC GACAGCTGGA TCGAGATGGA ATCATTCCGG CTGCTGGTGT TGCGGACCGC CTGGTTGATC GATAAGCACC AGGATTACTT GAAGGTACGC AAGGATATTT CGGCCATCAA GGCGGCGATG CCCAAGGTGA TGCACGATGT CGCCCAGCGC GCACTGCATC TGCATGGCTC ACTCGGGGTG TCCGAAGAGA TGCCGCTGGC CAAGATGTTT CTGTATTCGG AGGTCATGGG TCTGGTCGAC GGCCCGACCG AGGTACACAA GGTCACTATC GCCAAGGAAG TCCTCAAGGA CTACACCCCT TACGAGGGTC TGTTTCCGCC CAGCCACATC CCGGCATTGA CGGAGAAGGC GCGCGCCCAT GTCGCGGCCC GGCTCGAACA TCGGGTGGGA AATCTG
Details for MyabA.00309.c.A1.GE30288
HARVESTED ON: 8/31/2010
SEQUENCED ON: 9/7/2010
EXPECTED MW: 50kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MVTAMAWDFE TDPEYQELLD WADEFVRTEV EPLDYVFPNP YDKSDTVAMD YVRPLKEEVK RRGLWACHLG PELGGPGYGQ LKLALLNEIL GRSVWAPSIF GCQAPDTGNA EILAHYGTEA QKAKYLQPLM DGDIGSCYVM TEPQGGSDPT LFKTTAVRDG DEWILNGEKW FNSEARHAAF WIVMAVTNTE VSPYQGMSMF IVDPNAPGVE IVRNITVHGF SEDEAYTRFT NARIPAENML GAEGGAFAIA QTRLGGGRVH HAMRTVAQVR KAFDMMCERV VSREARGGTI AKLQMTQERI ADSWIEMESF RLLVLRTAWL IDKHQDYLKV RKDISAIKAA MPKVMHDVAQ RALHLHGSLG VSEEMPLAKM FLYSEVMGLV DGPTEVHKVT IAKEVLKDYT PYEGLFPPSH IPALTEKARA HVAARLEHRV G
Validated NT Sequence
ttcccacccg atgttcgagc cgggccgcga catgggcgcg cgccttctcc gtcaatgccg ggatgtggct gggcggaaac agaccctcgt aaggggtgta gtccttgagg acttccttgg cgatagtgac cttgtgtacc tcggtcgggc cgtcgaccag acccatgacc tccgaataca gaaacatctt ggccagcggc atctcttcgg acaccccgag tgagccatgc agatgcagtg cgcgctgggc gacatcgtgc atcaccttgg gcatcgccgc cttgatggcc gaaatatcct tgcgtacctt caagtaatcc tggtgcttat cgatcaacca ggcggtccgc aacaccagca gccggaatga ttccatctcg atccagctgt cggcgatacg ttcctgggtc atctggagtt tggcgatggt gccgcctcgg gcctcccggc tgacgacgcg ttcgcacatc atgtcgaagg ccttgcgtac ctgggccacc gtgcgcatgg cgtgatgtac acgaccacca ccgagacgcg tctgtgcgat ggcgaacgca cccccctcgg cacccagcat gttctcggca ggaatgcggg cgttggtgaa gcgggtgtag gcctcgtctt cactgaaccc gtgcaccgtg atgttccgga cgatctcgac accgggcgcg ttcgggtcga cgatgaacat cgacatcccc tgatagggac tgacttcggt gttggtgacg gccatgacga tccagaacgc ggcgtgccgc gcctcggagt tgaaccactt ctcaccattg aggatccatt cgtcaccgtc acggacggcg gtggtcttga acagcgtcgg atccgatccg ccctgcggct cggtcatcac ataacaggac ccgatgtcgc cgtccatcag tggctgcagg tatttggcct tctgcgcttc ggtgccgtag tgcgcgagga tctcggcatt gccggtatcg ggtgcctggc atccgaagat cgaaggagcc cacaccgacc tacccagaat ctcgttgagc agcgccagct tgagctggcc gtaacccgga ccacccagct ccggtcccag atgacatgcc cacaggccgc ggcgtttgac ctcttccttg agcggccgca cgtaatccat ggccaccgtg tcggacttgt cgtatgggtt ggggaacacg taatcgaggg gttcgacctc ggtgcggaca aactcgtcgg cccagtcaag cagttcctgg tattccgggt cggtttcgaa atcccatgcc atggcggtca ccatcgaacc aggaccctgg gtctgagctt ccagg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTAMAWDF ETDPEYQELL DWADEFVRTE VEPLDYVFPN PYDKSDTVAM DYVRPLKEEV KRRGLWACHL GPELGGPGYG QLKLALLNEI LGRSVWAPSI FGCQAPDTGN AEILAHYGTE AQKAKYLQPL MDGDIGSCYV MTEPQGGSDP TLFKTTAVRD GDEWILNGEK WFNSEARHAA FWIVMAVTNT EVSPYQGMSM FIVDPNAPGV EIVRNITVHG FSEDEAYTRF TNARIPAENM LGAEGGAFAI AQTRLGGGRV HHAMRTVAQV RKAFDMMCER VVSREARGGT IAKLQMTQER IADSWIEMES FRLLVLRTAW LIDKHQDYLK VRKDISAIKA AMPKVMHDVA QRALHLHGSL GVSEEMPLAK MFLYSEVMGL VDGPTEVHKV TIAKEVLKDY TPYEGLFPPS HIPALTEKAR AHVAARLEHR VGNL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaccgcc atggcatggg atttcgaaac cgacccggaa taccaggaac tgcttgactg ggccgacgag tttgtccgca ccgaggtcga acccctcgat tacgtgttcc ccaacccata cgacaagtcc gacacggtgg ccatggatta cgtgcggccg ctcaaggaag aggtcaaacg ccgcggcctg tgggcatgtc atctgggacc ggagctgggt ggtccgggtt acggccagct caagctggcg ctgctcaacg agattctggg taggtcggtg tgggctcctt cgatcttcgg atgccaggca cccgataccg gcaatgccga gatcctcgcg cactacggca ccgaagcgca gaaggccaaa tacctgcagc cactgatgga cggcgacatc gggtcctgtt atgtgatgac cgagccgcag ggcggatcgg atccgacgct gttcaagacc accgccgtcc gtgacggtga cgaatggatc ctcaatggtg agaagtggtt caactccgag gcgcggcacg ccgcgttctg gatcgtcatg gccgtcacca acaccgaagt cagtccctat caggggatgt cgatgttcat cgtcgacccg aacgcgcccg gtgtcgagat cgtccggaac atcacggtgc acgggttcag tgaagacgag gcctacaccc gcttcaccaa cgcccgcatt cctgccgaga acatgctggg tgccgagggg ggtgcgttcg ccatcgcaca gacgcgtctc ggtggtggtc gtgtacatca cgccatgcgc acggtggccc aggtacgcaa ggccttcgac atgatgtgcg aacgcgtcgt cagccgggag gcccgaggcg gcaccatcgc caaactccag atgacccagg aacgtatcgc cgacagctgg atcgagatgg aatcattccg gctgctggtg ttgcggaccg cctggttgat cgataagcac caggattact tgaaggtacg caaggatatt tcggccatca aggcggcgat gcccaaggtg atgcacgatg tcgcccagcg cgcactgcat ctgcatggct cactcggggt gtccgaagag atgccgctgg ccaagatgtt tctgtattcg gaggtcatgg gtctggtcga cggcccgacc gaggtacaca aggtcactat cgccaaggaa gtcctcaagg actacacccc ttacgagggt ctgtttccgc ccagccacat cccggcattg acggagaagg cgcgcgccca tgtcgcggcc cggctcgaac atcgggtggg aaatctgaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa