SSGCID: Target Page

MyabA.00305.d

Probable enoyl-CoA hydratase/isomerase

CENTER ID:	MyabA.00305.d
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.00305.d.A1.GE30285	Full length( MyabA.00305.d )		1	268

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|36809.5.peg.4644
RefSeq:	YP_001705293.1
UniProt:	B1MKZ4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSLDESVGAP DVAVDGGIMR VTFTRPDRMN ALNGPATSGL IEALESIPGR DDVRVVVISG GAPGSAFSAG ADVTELAAGS ADLTPLQAAE LTMDNAERLV RAVLNCPVPT IAEVTGVAAG IGASVGLACD LIYAADTAFF LLAFANIGLM PDGGSSALVS ASIGRVKANA MALLAQPLSA TDAFAAGLVN EALPGDQLRE RVDKTARRLA HGSRRAMQLT KEAMNAVSLK LFDEAIARER DGQIELLISP DAHEGFAAFL EGRRPNFS

NT Sequence

GTGAGTCTCG ATGAATCTGT CGGCGCACCC GATGTCGCCG TCGACGGCGG CATCATGCGG GTCACGTTCA CCCGGCCTGA CCGGATGAAT GCGCTCAATG GACCGGCCAC GTCCGGCCTG ATCGAGGCTT TGGAATCCAT CCCCGGCCGA GACGACGTAC GTGTCGTCGT CATCAGTGGC GGCGCACCGG GCAGCGCATT TTCGGCGGGC GCCGACGTCA CCGAATTGGC GGCCGGTTCG GCGGATCTGA CGCCGCTGCA GGCAGCCGAG CTGACCATGG ACAACGCCGA GCGCTTGGTG CGCGCGGTGC TGAATTGCCC GGTGCCCACC ATCGCAGAGG TCACCGGGGT GGCAGCCGGT ATCGGTGCTT CGGTGGGCCT GGCATGCGAT TTGATCTACG CCGCCGATAC TGCCTTCTTT CTCTTGGCCT TCGCCAATAT CGGGCTGATG CCCGACGGCG GATCGAGTGC GTTGGTGTCC GCTTCCATCG GGCGGGTGAA GGCCAACGCG ATGGCATTGC TGGCCCAACC ACTCAGCGCC ACAGATGCTT TCGCGGCCGG CCTGGTAAAT GAGGCATTGC CCGGGGACCA ACTGCGCGAG CGCGTCGACA AGACGGCGCG CCGCTTGGCC CACGGCTCCC GCCGCGCGAT GCAGCTGACC AAGGAGGCGA TGAACGCGGT ATCGCTCAAG CTGTTCGACG AGGCCATCGC CCGTGAACGT GACGGGCAGA TCGAGCTGCT GATTTCACCC GATGCCCACG AGGGTTTCGC CGCATTTCTT GAGGGCCGAC GCCCCAACTT CAGT

Details for MyabA.00305.d.A1.GE30285

HARVESTED ON:	8/31/2010
SEQUENCED ON:	9/7/2010
EXPECTED MW:	29kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVSLDESVG APDVAVDGGI MRVTFTRPDR MNALNGPATS GLIEALESIP GRDDVRVVVI SGGAPGSAFS AGADVTELAA GSADLTPLQA AELTMDNAER LVRAVLNCPV PTIAEVTGVA AGIGASVGLA CDLIYAADTA FFLLAFANIG LMPDGGSSAL VSASIGRVKA NAMALLAQPL SATDAFAAGL VNEALPGDQL RERVDKTARR LAHGSRRAMQ LTKEAMNAVS LKLFDEAIAR ERDGQIELLI SPDAHEGFAA FLEGRRPNFS

Validated NT Sequence

tattaactga agttggggcg tcggccctca agaaatgcgg cgaaaccctc gtgggcatcg ggtgaaatca gcagctcgat ctgcccgtca cgttcacggg cgatggcctc gtcgaacagc ttgagcgata ccgcgttcat cgcctccttg gtcagctgca tcgcgcggcg ggagccgtgg gccaagcggc gcgccgtctt gtcgacgcgc tcgcgcagtt ggtccccggg caatgcctca tttaccaggc cggccgcgaa agcatctgtg gcgctgagtg gttgggccag caatgccatc gcgttggcct tcacccgccc gatggaagcg gacaccaacg cactcgatcc gccgtcgggc atcagcccga tattggcgaa ggccaagaga aagaaggcag tatcggcggc gtagatcaaa tcgcatgcca ggcccaccga agcaccgata ccggctgcca ccccggtgac ctctgcgatg gtgggcaccg ggcaattcag caccgcgcgc accaagcgct cggcgttgtc catggtcagc tcggctgcct gcagcggcgt cagatccgcc gaaccggccg ccaattcggt gacgtcggcg cccgccgaaa atgcgctgcc cggtgcgccg ccactgatga cgacgacacg tacgtcgtct cggccgggga tggattccaa agcctcgatc aggccggacg tggccggtcc attgagcgca ttcatccggt caggccgggt gaacgtgacc cgcatgatgc cgccgtcgac ggcgacatcg ggtgcgccga cagattcatc gagactcacc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagtctc gatgaatctg tcggcgcacc cgatgtcgcc gtcgacggcg gcatcatgcg ggtcacgttc acccggcctg accggatgaa tgcgctcaat ggaccggcca cgtccggcct gatcgaggct ttggaatcca tccccggccg agacgacgta cgtgtcgtcg tcatcagtgg cggcgcaccg ggcagcgcat tttcggcggg cgccgacgtc accgaattgg cggccggttc ggcggatctg acgccgctgc aggcagccga gctgaccatg gacaacgccg agcgcttggt gcgcgcggtg ctgaattgcc cggtgcccac catcgcagag gtcaccgggg tggcagccgg tatcggtgct tcggtgggcc tggcatgcga tttgatctac gccgccgata ctgccttctt tctcttggcc ttcgccaata tcgggctgat gcccgacggc ggatcgagtg cgttggtgtc cgcttccatc gggcgggtga aggccaacgc gatggcattg ctggcccaac cactcagcgc cacagatgct ttcgcggccg gcctggtaaa tgaggcattg cccggggacc aactgcgcga gcgcgtcgac aagacggcgc gccgcttggc ccacggctcc cgccgcgcga tgcagctgac caaggaggcg atgaacgcgg tatcgctcaa gctgttcgac gaggccatcg cccgtgaacg tgacgggcag atcgagctgc tgatttcacc cgatgcccac gagggtttcg ccgcatttct tgagggccga cgccccaact tcagtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa