MyabA.00305.c
Putative enoyl-CoA hydratase/isomerase
| CENTER ID: | MyabA.00305.c |
| ORGANISM: | Mycobacterium abscessus ATCC 19977 / DSM 44196 |
| ASSOCIATED DISEASE: | Skin abscess |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyabA.00305.c.A1.GE34905 | Full length( MyabA.00305.c ) | 1 | 267 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyabA.00305.c.A1.PW35520 | Full length( MyabA.00305.c ) | 1 | 267 |
Structures
4MI2
DEPOSITED: 8/30/2013
DETERMINATION: XRay
CLONE: MyabA.00305.c.A1.GE34905
PROTEIN: MyabA.00305.c.A1.PW35520
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|36809.5.peg.942 |
| RefSeq: | YP_001701653.1 |
| UniProt: | B1MJ43 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTQTAPAAVA YSVNHAGVAA IVLDRPEASN ALDRTMKTEL LQALLAAGGD PAVRAVVMSA AGKNFCVGQD LAEHVEALRD DPAHAMDTVR EHYNPVLEAL DAIKVPVVVA INGACVGAGL GLALGADIRI AGQRAKFGTA FTGIGLAADS ALSASLPRLI GASRATAMFL LGDTIDAPTA HTWGLVHEVV DEGSPADVAN SVAGRLAGGP TAAFSEVKEL LRRNAVAPLG DVLEREASAQ QRLGASRDHS AAVEAFLAKD KPVFVGR
NT Sequence
ATGACTCAAA CCGCACCGGC CGCCGTCGCG TACTCAGTGA ACCACGCTGG CGTCGCTGCG ATCGTGCTCG ATCGGCCGGA GGCGTCGAAC GCGCTTGACC GGACGATGAA AACCGAACTG CTGCAGGCTT TGTTGGCAGC GGGCGGTGAT CCAGCGGTAC GCGCGGTCGT CATGAGCGCA GCCGGTAAGA ACTTCTGCGT CGGCCAGGAT CTTGCCGAGC ATGTCGAGGC GCTGCGCGAC GACCCGGCTC ACGCGATGGA CACCGTGCGC GAGCACTACA ACCCGGTCCT GGAGGCGTTG GATGCCATCA AGGTGCCGGT CGTCGTGGCG ATCAACGGCG CCTGTGTGGG CGCGGGGTTG GGCTTGGCGT TGGGTGCCGA TATCCGTATC GCCGGTCAGC GCGCCAAGTT CGGGACCGCT TTCACCGGCA TCGGCCTAGC CGCGGATTCC GCGTTGTCGG CGTCGTTGCC AAGGCTCATC GGAGCTAGCC GGGCGACGGC CATGTTCCTG CTCGGTGACA CCATCGATGC CCCGACGGCC CACACGTGGG GGCTGGTGCA CGAGGTGGTT GACGAGGGCT CACCCGCCGA CGTAGCGAAC TCCGTCGCCG GTCGCCTGGC CGGCGGGCCC ACCGCCGCCT TCTCAGAGGT CAAGGAACTG TTGCGTCGCA ACGCTGTCGC GCCACTAGGC GATGTCCTAG AACGGGAAGC GAGCGCCCAG CAGCGCCTCG GGGCGTCCCG CGACCACAGC GCTGCGGTGG AGGCATTCCT GGCCAAGGAC AAGCCGGTCT TCGTCGGCCG C
Details for MyabA.00305.c.A1.GE34905
| HARVESTED ON: | 4/17/2012 |
| SEQUENCED ON: | 4/17/2012 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 30kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTQTAPAAV AYSVNHAGVA AIVLDRPEAS NALDRTMKTE LLQALLAAGG DPAVRAVVMS AAGKNFCVGQ DLAEHVEALR DDPAHAMDTV REHYNPVLEA LDAIKVPVVV AINGACVGAG LGLALGADIR IAGQRAKFGT AFTGIGLAAD SALSASLPRL IGASRATAMF LLGDTIDAPT AHTWGLVHEV VDEGSPADVA NSVAGRLAGG PTAAFSEVKE LLRRNAVAPL GDVLEREASA QQRLGASRDH SAAVEAFLAK DKPVFVGR
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgactc aaaccgcacc ggccgccgtc gcgtactcag tgaaccacgc tggcgtcgct gcgatcgtgc tcgatcggcc ggaggcgtcg aacgcgcttg accggacgat gaaaaccgaa ctgctgcagg ctttgttggc agcgggcggt gatccagcgg tacgcgcggt cgtcatgagc gcagccggta agaacttctg cgtcggccag gatcttgccg agcatgtcga ggcgctgcgc gacgacccgg ctcacgcgat ggacaccgtg cgcgagcact acaacccggt cctggaggcg ttggatgcca tcaaggtgcc ggtcgtcgtg gcgatcaacg gcgcctgtgt gggcgcgggg ttgggcttgg cgttgggtgc cgatatccgt atcgccggtc agcgcgccaa gttcgggacc gctttcaccg gcatcggcct agccgcggat tccgcgttgt cggcgtcgtt gccaaggctc atcggagcta gccgggcgac ggccatgttc ctgctcggtg acaccatcga tgccccgacg gcccacacgt gggggctggt gcacgaggtg gttgacgagg gctcacccgc cgacgtagcg aactccgtcg ccggtcgcct ggccggcggg cccaccgccg ccttctcaga ggtcaaggaa ctgttgcgtc gcaacgctgt cgcgccacta ggcgatgtcc tagaacggga agcgagcgcc cagcagcgcc tcggggcgtc ccgcgaccac agcgctgcgg tggaggcatt cctggccaag gacaagccgg tcttcgtcgg ccgctaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTQTAPAAV AYSVNHAGVA AIVLDRPEAS NALDRTMKTE LLQALLAAGG DPAVRAVVMS AAGKNFCVGQ DLAEHVEALR DDPAHAMDTV REHYNPVLEA LDAIKVPVVV AINGACVGAG LGLALGADIR IAGQRAKFGT AFTGIGLAAD SALSASLPRL IGASRATAMF LLGDTIDAPT AHTWGLVHEV VDEGSPADVA NSVAGRLAGG PTAAFSEVKE LLRRNAVAPL GDVLEREASA QQRLGASRDH SAAVEAFLAK DKPVFVGR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gactcaaacc gcaccggccg ccgtcgcgta ctcagtgaac cacgctggcg tcgctgcgat cgtgctcgat cggccggagg cgtcgaacgc gcttgaccgg acgatgaaaa ccgaactgct gcaggctttg ttggcagcgg gcggtgatcc agcggtacgc gcggtcgtca tgagcgcagc cggtaagaac ttctgcgtcg gccaggatct tgccgagcat gtcgaggcgc tgcgcgacga cccggctcac gcgatggaca ccgtgcgcga gcactacaac ccggtcctgg aggcgttgga tgccatcaag gtgccggtcg tcgtggcgat caacggcgcc tgtgtgggcg cggggttggg cttggcgttg ggtgccgata tccgtatcgc cggtcagcgc gccaagttcg ggaccgcttt caccggcatc ggcctagccg cggattccgc gttgtcggcg tcgttgccaa ggctcatcgg agctagccgg gcgacggcca tgttcctgct cggtgacacc atcgatgccc cgacggccca cacgtggggg ctggtgcacg aggtggttga cgagggctca cccgccgacg tagcgaactc cgtcgccggt cgcctggccg gcgggcccac cgccgccttc tcagaggtca aggaactgtt gcgtcgcaac gctgtcgcgc cactaggcga tgtcctagaa cgggaagcga gcgcccagca gcgcctcggg gcgtcccgcg accacagcgc tgcggtggag gcattcctgg ccaaggacaa gccggtcttc gtcggccgca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for MyabA.00305.c.A1.PW35520
| PURIFICATION DATe: | 6/22/2012 |
| CONCENTRATION: | 51.53mg/ml |
| OBSERVED MW: | 28kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTQTAPAAV AYSVNHAGVA AIVLDRPEAS NALDRTMKTE LLQALLAAGG DPAVRAVVMS AAGKNFCVGQ DLAEHVEALR DDPAHAMDTV REHYNPVLEA LDAIKVPVVV AINGACVGAG LGLALGADIR IAGQRAKFGT AFTGIGLAAD SALSASLPRL IGASRATAMF LLGDTIDAPT AHTWGLVHEV VDEGSPADVA NSVAGRLAGG PTAAFSEVKE LLRRNAVAPL GDVLEREASA QQRLGASRDH SAAVEAFLAK DKPVFVGR
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgactc aaaccgcacc ggccgccgtc gcgtactcag tgaaccacgc tggcgtcgct gcgatcgtgc tcgatcggcc ggaggcgtcg aacgcgcttg accggacgat gaaaaccgaa ctgctgcagg ctttgttggc agcgggcggt gatccagcgg tacgcgcggt cgtcatgagc gcagccggta agaacttctg cgtcggccag gatcttgccg agcatgtcga ggcgctgcgc gacgacccgg ctcacgcgat ggacaccgtg cgcgagcact acaacccggt cctggaggcg ttggatgcca tcaaggtgcc ggtcgtcgtg gcgatcaacg gcgcctgtgt gggcgcgggg ttgggcttgg cgttgggtgc cgatatccgt atcgccggtc agcgcgccaa gttcgggacc gctttcaccg gcatcggcct agccgcggat tccgcgttgt cggcgtcgtt gccaaggctc atcggagcta gccgggcgac ggccatgttc ctgctcggtg acaccatcga tgccccgacg gcccacacgt gggggctggt gcacgaggtg gttgacgagg gctcacccgc cgacgtagcg aactccgtcg ccggtcgcct ggccggcggg cccaccgccg ccttctcaga ggtcaaggaa ctgttgcgtc gcaacgctgt cgcgccacta ggcgatgtcc tagaacggga agcgagcgcc cagcagcgcc tcggggcgtc ccgcgaccac agcgctgcgg tggaggcatt cctggccaag gacaagccgg tcttcgtcgg ccgctaataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTQTAPAAV AYSVNHAGVA AIVLDRPEAS NALDRTMKTE LLQALLAAGG DPAVRAVVMS AAGKNFCVGQ DLAEHVEALR DDPAHAMDTV REHYNPVLEA LDAIKVPVVV AINGACVGAG LGLALGADIR IAGQRAKFGT AFTGIGLAAD SALSASLPRL IGASRATAMF LLGDTIDAPT AHTWGLVHEV VDEGSPADVA NSVAGRLAGG PTAAFSEVKE LLRRNAVAPL GDVLEREASA QQRLGASRDH SAAVEAFLAK DKPVFVGR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACTCAAACC GCACCGGCCG CCGTCGCGTA CTCAGTGAAC CACGCTGGCG TCGCTGCGAT CGTGCTCGAT CGGCCGGAGG CGTCGAACGC GCTTGACCGG ACGATGAAAA CCGAACTGCT GCAGGCTTTG TTGGCAGCGG GCGGTGATCC AGCGGTACGC GCGGTCGTCA TGAGCGCAGC CGGTAAGAAC TTCTGCGTCG GCCAGGATCT TGCCGAGCAT GTCGAGGCGC TGCGCGACGA CCCGGCTCAC GCGATGGACA CCGTGCGCGA GCACTACAAC CCGGTCCTGG AGGCGTTGGA TGCCATCAAG GTGCCGGTCG TCGTGGCGAT CAACGGCGCC TGTGTGGGCG CGGGGTTGGG CTTGGCGTTG GGTGCCGATA TCCGTATCGC CGGTCAGCGC GCCAAGTTCG GGACCGCTTT CACCGGCATC GGCCTAGCCG CGGATTCCGC GTTGTCGGCG TCGTTGCCAA GGCTCATCGG AGCTAGCCGG GCGACGGCCA TGTTCCTGCT CGGTGACACC ATCGATGCCC CGACGGCCCA CACGTGGGGG CTGGTGCACG AGGTGGTTGA CGAGGGCTCA CCCGCCGACG TAGCGAACTC CGTCGCCGGT CGCCTGGCCG GCGGGCCCAC CGCCGCCTTC TCAGAGGTCA AGGAACTGTT GCGTCGCAAC GCTGTCGCGC CACTAGGCGA TGTCCTAGAA CGGGAAGCGA GCGCCCAGCA GCGCCTCGGG GCGTCCCGCG ACCACAGCGC TGCGGTGGAG GCATTCCTGG CCAAGGACAA GCCGGTCTTC GTCGGCCGCA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A