SSGCID: Target Page

MyabA.00272.a

8-amino-7-oxononanoate synthase (BioF)

CENTER ID:	MyabA.00272.a
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.00272.a.A1.GE30277	Full length( MyabA.00272.a )		1	383

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|36809.5.peg.2731
RefSeq:	YP_001703421.1
UniProt:	B1MBZ6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPVVEASSPL AWLGEVERQR EAAGLRRRLR TRSAAEPEID LASNDYLGLS RHPQVIEAGV EALRIWGAGS TGSRLVTGNT ELHEELEQEL ATFMGTESAL VFSSGYTANL GAVVALSGPG TLIVSDARTH ASLIDACRLS RARVVVTPYR DTQAVAAALA QRPEERALVL TDAVFSADGA LAPLAELYGV CRRHGAVLLV DEAHGLGVRG SGGRGLVAEM NLAGRDDLVV TVTLSKALGS QGGAVLGSAA VRAHLIDAAR PFIFDTGLAP AAVGSALAAL RLMIDEPARI RAVLAHAAAL GQWCGVPDKP ESAVVPVVLG DPTVAFNAAR SCLEQGVRVG CFRPPSVPEG QSLLRLTARA SLTEADMERV HEVLAGVLTL ARR

NT Sequence

GTGCCGGTGG TCGAGGCATC ATCACCGCTT GCCTGGTTGG GTGAGGTCGA GCGGCAACGG GAAGCTGCCG GGCTGCGTCG ACGGCTGCGT ACGCGCAGCG CGGCAGAACC CGAAATCGAC CTGGCTTCCA ATGACTACCT GGGCCTTTCC CGGCATCCGC AGGTCATCGA GGCAGGTGTC GAGGCGTTGC GGATATGGGG TGCCGGATCC ACCGGCTCAC GTCTGGTCAC CGGTAACACC GAGCTGCATG AAGAACTCGA GCAAGAGCTC GCGACCTTCA TGGGTACGGA GTCGGCGCTG GTCTTCTCCT CGGGATACAC CGCCAACCTC GGGGCGGTGG TCGCCCTGTC CGGCCCGGGG ACCCTCATCG TCTCCGATGC GCGCACGCAT GCCTCGCTGA TCGATGCCTG CCGTTTGTCG CGCGCGCGGG TGGTGGTGAC CCCCTACCGC GACACGCAAG CGGTGGCGGC GGCCCTGGCG CAGCGCCCCG AAGAACGTGC GCTGGTGCTC ACCGACGCGG TCTTCAGCGC CGACGGTGCG CTGGCCCCGT TGGCCGAACT GTATGGCGTG TGCCGCCGCC ACGGCGCAGT GCTGCTGGTC GACGAGGCGC ACGGGCTTGG TGTGCGTGGG TCGGGTGGCC GCGGTCTGGT CGCCGAAATG AACCTGGCGG GCCGGGATGA CCTCGTCGTG ACCGTGACGC TTTCCAAGGC GCTGGGTAGT CAGGGCGGGG CCGTGCTCGG ATCGGCGGCG GTGCGTGCGC ACCTCATTGA CGCCGCCCGT CCGTTCATCT TCGACACTGG GCTCGCGCCC GCGGCGGTCG GGTCGGCGTT GGCGGCCCTG CGCCTGATGA TCGACGAACC CGCGCGGATA CGTGCTGTCC TGGCGCATGC GGCGGCGCTG GGGCAGTGGT GCGGCGTGCC CGATAAGCCG GAATCGGCGG TGGTGCCGGT GGTTCTCGGT GATCCCACGG TCGCGTTCAA TGCCGCGCGG TCATGTTTGG AGCAGGGCGT TCGGGTGGGT TGTTTTCGTC CGCCCTCCGT CCCGGAAGGT CAGTCGCTGT TGCGACTGAC GGCCCGCGCC TCGCTCACCG AGGCCGATAT GGAACGCGTG CACGAGGTGC TTGCCGGGGT GCTGACGCTG GCGCGGCGA

Details for MyabA.00272.a.A1.GE30277

HARVESTED ON:	8/31/2010
SEQUENCED ON:	9/7/2010
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Validated AA Sequence

MVPVVEASSP LAWLGEVERQ REAAGLRRRL RTRSAAEPEI DLASNDYLGL SRHPQVIEAG VEALRIWGAG STGSRLVTGN TELHEELEQE LATFMGTESA LVFSSGYTAN LGAVVALSGP GTLIVSDART HASLIDACRL SRARVVVTPY RDTQAVAAAL AQRPEERALV LTDAVFSADG ALAPLAELYG VCRRHGAVLL VDEAHGLGVR GSGGRGLVAE MNLAGRDDLV VTVTLSKALG SQGGAVLGSA AVRAHLIDAA RPFIFDTGLA PAAVGSALAA LRLMIDEPAR IRAVLAHAAA LGQWCGVPDK PESAVVPVVL GDPTVAFNAA RSCLEQGVRV GCFRPPSVPE GQSLLRLTAR ASLTEADMER VHEVLAGVLT LAR

Validated NT Sequence

tcgccgcgcc agcgtcagca ccccggcaag cacctcgtgc acgcgttcca tatcggcctc ggtgagcgag gcgcgggccg tcagtcgcaa cagcgactga ccttccggga cggagggcgg acgaaaacaa cccacccgaa cgccctgctc caaacatgac cgcgcggcat tgaacgcgac cgtgggatca ccgagaacca ccggcaccac cgccgattcc ggcttatcgg gcacgccgca ccactgcccc agcgccgccg catgcgccag gacagcacgt atccgcgcgg gttcgtcgat catcaggcgc agggccgcca acgccgaccc gaccgccgcg ggcgcgagcc cagtgtcgaa gatgaacgga cgggcggcgt caatgaggtg cgcacgcacc gccgccgatc cgagcacggc cccgccctga ctacccagcg ccttggaaag cgtcacggtc acgacgaggt catcccggcc cgccaggttc atttcggcga ccagaccgcg gccacccgac ccacgcacac caagcccgtg cgcctcgtcg accagcagca ctgcgccgtg gcggcggcac acgccataca gttcggccaa cggggccagc gcaccgtcgg cgctgaagac cgcgtcggtg agcaccagcg cacgttcttc ggggcgctgc gccagggccg ccgccaccgc ttgcgtgtcg cggtaggggg tcaccaccac ccgcgcgcgc gacaaacggc aggcatcgat cagcgaggca tgcgtgcgcg catcggagac gatgagggtc cccgggccgg acagggcgac caccgccccg aggttggcgg tgtatcccga ggagaagacc agcgccgact ccgtacccat gaaggtcgcg agctcttgct cgagttcttc atgcagctcg gtgttaccgg tgaccagacg tgagccggtg gatccggcac cccatatccg caacgcctcg acacctgcct cgatgacctg cggatgccgg gaaaggccca ggtagtcatt ggaagccagg tcgatttcgg gttctgccgc gctgcgcgta cgcagccgtc gacgcagccc ggcagcttcc cgttgccgct cgacctcacc caaccaggca agcggtgatg atgcctcgac caccggcacc atcgaaccag gaccctgggt ctgagcttcc agg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVPVVEASS PLAWLGEVER QREAAGLRRR LRTRSAAEPE IDLASNDYLG LSRHPQVIEA GVEALRIWGA GSTGSRLVTG NTELHEELEQ ELATFMGTES ALVFSSGYTA NLGAVVALSG PGTLIVSDAR THASLIDACR LSRARVVVTP YRDTQAVAAA LAQRPEERAL VLTDAVFSAD GALAPLAELY GVCRRHGAVL LVDEAHGLGV RGSGGRGLVA EMNLAGRDDL VVTVTLSKAL GSQGGAVLGS AAVRAHLIDA ARPFIFDTGL APAAVGSALA ALRLMIDEPA RIRAVLAHAA ALGQWCGVPD KPESAVVPVV LGDPTVAFNA ARSCLEQGVR VGCFRPPSVP EGQSLLRLTA RASLTEADME RVHEVLAGVL TLARR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgccggtg gtcgaggcat catcaccgct tgcctggttg ggtgaggtcg agcggcaacg ggaagctgcc gggctgcgtc gacggctgcg tacgcgcagc gcggcagaac ccgaaatcga cctggcttcc aatgactacc tgggcctttc ccggcatccg caggtcatcg aggcaggtgt cgaggcgttg cggatatggg gtgccggatc caccggctca cgtctggtca ccggtaacac cgagctgcat gaagaactcg agcaagagct cgcgaccttc atgggtacgg agtcggcgct ggtcttctcc tcgggataca ccgccaacct cggggcggtg gtcgccctgt ccggcccggg gaccctcatc gtctccgatg cgcgcacgca tgcctcgctg atcgatgcct gccgtttgtc gcgcgcgcgg gtggtggtga ccccctaccg cgacacgcaa gcggtggcgg cggccctggc gcagcgcccc gaagaacgtg cgctggtgct caccgacgcg gtcttcagcg ccgacggtgc gctggccccg ttggccgaac tgtatggcgt gtgccgccgc cacggcgcag tgctgctggt cgacgaggcg cacgggcttg gtgtgcgtgg gtcgggtggc cgcggtctgg tcgccgaaat gaacctggcg ggccgggatg acctcgtcgt gaccgtgacg ctttccaagg cgctgggtag tcagggcggg gccgtgctcg gatcggcggc ggtgcgtgcg cacctcattg acgccgcccg tccgttcatc ttcgacactg ggctcgcgcc cgcggcggtc gggtcggcgt tggcggccct gcgcctgatg atcgacgaac ccgcgcggat acgtgctgtc ctggcgcatg cggcggcgct ggggcagtgg tgcggcgtgc ccgataagcc ggaatcggcg gtggtgccgg tggttctcgg tgatcccacg gtcgcgttca atgccgcgcg gtcatgtttg gagcagggcg ttcgggtggg ttgttttcgt ccgccctccg tcccggaagg tcagtcgctg ttgcgactga cggcccgcgc ctcgctcacc gaggccgata tggaacgcgt gcacgaggtg cttgccgggg tgctgacgct ggcgcggcga aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa