SSGCID: Target Page

MyabA.00211.a

Pyrroline-5-carboxylate reductase (Rv0500 ortholog)

CENTER ID:	MyabA.00211.a
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.00211.a.B1.GE37291	full length		1	299

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|36809.5.peg.4080
RefSeq:	YP_001704733.1
UniProt:	B1MHJ8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLVAMSRIAI IGGGNIGEAL ISGLLRAGRQ AKDIVVSEKV PARARALADA YSIRISEVAD AVEGADFIVV AVKPSDVESA TTEIAAALAK LDAEGSERET EQVLVSVAAG VSTGFFESKL AAGAPVVRVM PNAPMLVGAG VSALAKGRFA NDEQLSAVAE LLESVGTVIT VAESQMDTVT AISGSGPAYF FLLVEALVDA GVASGLTRPV ATDLVIQTMA GSAAMLLERA ESDENRAPGL QTRTEVDTTA AELRATITSP GGTTAAALRE LERGGLRASV YAAVDAAKTR SEQLGITSE

NT Sequence

atgctggtcg ccatgtccag aatcgcaatt atcggtggcg gcaatatcgg cgaagcactc atctccgggc tgctgagggc cgggcggcag gccaaggaca ttgttgtctc ggagaaggta ccggcacggg ccagggccct tgccgacgcg tactccatcc ggatcagcga ggtggccgac gccgtcgagg gcgccgactt catcgtggtg gcggtcaaac cctccgatgt ggagagcgca acgaccgaga tcgccgcggc gctggcgaaa ttggacgccg agggcagcga gcgggaaacc gagcaggttc tggtgtcggt ggccgccggg gtatcgacgg gattctttga atccaagctg gcggccggag caccggtggt gcgcgtgatg cccaatgccc cgatgctggt cggtgcgggg gtaagcgcac tggccaaggg ccgattcgcc aatgatgagc agctcagtgc cgtcgccgag ctgctcgagt ccgtcggcac cgtcatcacc gtcgccgaat cgcagatgga caccgtgacc gccatttccg gatcagggcc ggcttacttc tttttgctcg tcgaggcgct ggtcgacgcg ggggtggcct cgggcctcac ccgccccgtc gcaaccgatc tggtcatcca aaccatggcc ggatccgccg cgatgctctt ggaacgggcc gaatcggacg agaatcgcgc ccccggactg cagacccgca ccgaggtgga tacgaccgcc gccgagctac gcgcgacgat tacctcgccg ggcggtacta ccgccgctgc gctgcgtgaa ctggaacgcg gaggtttgcg ggcaagcgtc tacgcggccg tggatgccgc aaaaacacgc tccgagcagc tcggaatcac atcagagtaa

Details for MyabA.00211.a.B1.GE37291

HARVESTED ON:	8/28/2013
SEQUENCED ON:	9/3/2013
EXPECTED MW:	32kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHML VAMSRIAIIG GGNIGEALIS GLLRAGRQAK DIVVSEKVPA RARALADAYS IRISEVADAV EGADFIVVAV KPSDVESATT EIAAALAKLD AEGSERETEQ VLVSVAAGVS TGFFESKLAA GAPVVRVMPN APMLVGAGVS ALAKGRFAND EQLSAVAELL ESVGTVITVA ESQMDTVTAI SGSGPAYFFL LVEALVDAGV ASGLTRPVAT DLVIQTMAGS AAMLLERAES DENRAPGLQT RTEVDTTAAE LRATITSPGG TTAAALRELE RGGLRASVYA AVDAXKTRS

Validated NT Sequence

cggagcgtgt ttttncggca tccacggccg cgtagacgct tgcccgcaaa cctccgcgtt ccagttcacg cagcgcagcg gcggtagtac cgcccggcga ggtaatcgtc gcgcgtagct cggcggcggt cgtatccacc tcggtgcggg tctgcagtcc gggggcgcga ttctcgtccg attcggcccg ttccaagagc atcgcggcgg atccggccat ggtttggatg accagatcgg ttgcgacggg gcgggtgagg cccgaggcca cccccgcgtc gaccagcgcc tcgacgagca aaaagaagta agccggccct gatccggaaa tggcggtcac ggtgtccatc tgcgattcgg cgacggtgat gacggtgccg acggactcga gcagctcggc gacggcactg agctgctcat cattggcgaa tcggcccttg gccagtgcgc ttacccccgc accgaccagc atcggggcat tgggcatcac gcgcaccacc ggtgctccgg ccgccagctt ggattcaaag aatcccgtcg ataccccggc ggccaccgac accagaacct gctcggtttc ccgctcgctg ccctcggcgt ccaatttcgc cagcgccgcg gcgatctcgg tcgttgcgct ctccacatcg gagggtttga ccgccaccac gatgaagtcg gcgccctcga cggcgtcggc cacctcgctg atccggatgg agtacgcgtc ggcaagggcc ctggcccgtg ccggtacctt ctccgagaca acaatgtcct tggcctgccg cccggccctc agcagcccgg agatgagtgc ttcgccgata ttgccgccac cgataattgc gattctggac atggcgacca gcatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctgg tcgccatgtc cagaatcgca attatcggtg gcggcaatat cggcgaagca ctcatctccg ggctgctgag ggccgggcgg caggccaagg acattgttgt ctcggagaag gtaccggcac gggccagggc ccttgccgac gcgtactcca tccggatcag cgaggtggcc gacgccgtcg agggcgccga cttcatcgtg gtggcggtca aaccctccga tgtggagagc gcaacgaccg agatcgccgc ggcgctggcg aaattggacg ccgagggcag cgagcgggaa accgagcagg ttctggtgtc ggtggccgcc ggggtatcga cgggattctt tgaatccaag ctggcggccg gagcaccggt ggtgcgcgtg atgcccaatg ccccgatgct ggtcggtgcg ggggtaagcg cactggccaa gggccgattc gccaatgatg agcagctcag tgccgtcgcc gagctgctcg agtccgtcgg caccgtcatc accgtcgccg aatcgcagat ggacaccgtg accgccattt ccggatcagg gccggcttac ttctttttgc tcgtcgaggc gctggtcgac gcgggggtgg cctcgggcct cacccgcccc gtcgcaaccg atctggtcat ccaaaccatg gccggatccg ccgcgatgct cttggaacgg gccgaatcgg acgagaatcg cgcccccgga ctgcagaccc gcaccgaggt ggatacgacc gccgccgagc tacgcgcgac gattacctcg ccgggcggta ctaccgccgc tgcgctgcgt gaactggaac gcggaggttt gcgggcaagc gtctacgcgg ccgtggatgc cgcaaaaaca cgctccgagc agctcggaat cacatcagag tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat