MyabA.00185.c

Probable acyl-CoA dehydrogenase FadE

CENTER ID: MyabA.00185.c
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00185.c.A1.GE34915 Full length( MyabA.00185.c ) 1 614
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00185.c.A1.PW35530 Full length( MyabA.00185.c ) 1 614

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.4512
RefSeq: YP_001705161.1
UniProt: B1MK00

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTHMGHYKSN VRDLEFNLFE LFKIQQVFGG EEFPELDEDT ARTFLAEMRT LAEGPLADSF AEGDRNPPVF DPETHSVAIP EAFKKSVKAI TEAGWDRVGL QEELGGTPVP RSLSWAIQEM ILGANPAVWM YSGGAGFAQI FYNIATDEQK KWAEFVAERG WGATMVLTEP DAGSDVGAGR TKAVKQDDGS WHIDGVKRFI TSADSDDMFE NIMHLVLARP EGAGPGTKGL SLFFVPKFIP NFETGEPGER NGVFVTNVEH KMGLKVSATC ELSFGQHGVP AKGWLVGEVH NGIAQMFDVI EQARMMVGTK AIATLSTGYL NALEYAKTRV QGADMTQLTD KTAPRVTITH HPDVRRSLMT QKVYAEGLRA LYLYATTFQD AVAAKTINGV EAEDAVKLND LLLPVIKGVG SERAYEKLTE SLQTFGGSGF LQDYPIEQYI RDAKIDSLYE GTTAIQAQDF FFRKIVKDQG KSLAFISGQI EEFVKSETGN GRLKAERALL ATALEDVQGM AASMTGNLMA AQQEITQVYR VGTASVRFLM SVGDLLIGWL LQRQAAVAIE ALDAGATGAD KSFYEGKIAA ASFFAKNMLP LLTSTRAVLE NVDNDIMELD EAAF
NT Sequence
ATGACGCACA TGGGCCACTA CAAGTCGAAC GTCCGCGACC TGGAGTTCAA CCTCTTCGAG CTCTTCAAGA TCCAGCAGGT ATTCGGCGGC GAGGAATTCC CCGAGCTGGA CGAAGACACC GCTCGCACCT TCCTTGCCGA GATGCGCACC CTCGCCGAAG GGCCGCTGGC CGATTCATTC GCCGAAGGCG ACCGCAACCC GCCGGTTTTC GATCCGGAGA CCCACTCGGT GGCCATCCCC GAAGCCTTCA AGAAGTCCGT CAAGGCCATC ACCGAGGCCG GCTGGGACCG CGTCGGACTC CAGGAAGAGC TCGGCGGCAC CCCCGTGCCG CGGTCGCTGT CCTGGGCCAT CCAAGAGATG ATCCTGGGCG CCAACCCCGC TGTCTGGATG TACAGCGGCG GTGCCGGTTT CGCGCAGATC TTCTACAACA TCGCCACCGA TGAGCAGAAG AAGTGGGCCG AGTTCGTCGC CGAGCGCGGC TGGGGAGCCA CCATGGTGCT CACCGAGCCC GACGCCGGTT CGGATGTGGG CGCCGGCCGC ACCAAGGCCG TCAAGCAGGA CGACGGCTCA TGGCACATCG ACGGTGTGAA GCGCTTCATC ACCTCGGCCG ACTCGGACGA CATGTTCGAG AACATCATGC ATCTGGTGCT GGCTCGGCCC GAGGGCGCCG GCCCGGGCAC CAAGGGGCTG TCGCTGTTCT TCGTGCCGAA GTTCATCCCC AACTTCGAAA CCGGCGAGCC CGGCGAGCGC AACGGTGTGT TCGTCACCAA CGTCGAGCAC AAGATGGGCC TGAAGGTTTC GGCTACCTGT GAGCTGAGCT TCGGTCAGCA CGGCGTACCC GCCAAGGGCT GGCTGGTCGG CGAGGTACAC AACGGTATTG CGCAGATGTT CGATGTCATC GAGCAGGCCC GCATGATGGT GGGCACCAAG GCCATCGCCA CGCTGTCGAC CGGCTACCTG AACGCGCTGG AATACGCCAA GACCCGCGTG CAGGGTGCGG ACATGACGCA GCTGACCGAC AAGACCGCTC CCCGGGTCAC CATCACGCAC CACCCGGACG TGCGTCGCTC GCTGATGACT CAGAAGGTTT ACGCCGAGGG TCTGCGTGCG CTGTACCTCT ACGCCACCAC CTTCCAGGAT GCCGTGGCGG CCAAGACCAT CAACGGTGTC GAGGCCGAGG ACGCGGTCAA GCTCAACGAC CTGCTGCTGC CGGTCATCAA GGGTGTGGGC TCCGAGCGCG CCTACGAGAA GCTCACCGAG AGCCTGCAGA CCTTCGGTGG CTCCGGCTTC CTGCAGGACT ACCCGATCGA GCAGTACATC CGCGATGCCA AGATCGACTC GCTGTACGAG GGCACCACGG CAATCCAGGC CCAGGACTTC TTCTTCCGGA AGATCGTCAA GGACCAGGGC AAGTCGCTGG CCTTCATTTC CGGCCAGATC GAGGAGTTCG TCAAGAGCGA GACCGGAAAC GGCCGCCTCA AGGCCGAGCG TGCGCTGCTC GCGACGGCCC TGGAGGACGT GCAGGGTATG GCGGCAAGCA TGACTGGCAA CCTGATGGCT GCCCAGCAGG AGATCACCCA GGTCTACAGG GTCGGGACCG CCTCCGTGCG CTTCCTGATG AGCGTGGGCG ACCTGCTGAT CGGGTGGCTG CTGCAGCGTC AGGCCGCCGT GGCCATCGAG GCTCTCGATG CCGGTGCCAC CGGTGCCGAC AAGTCCTTCT ACGAGGGCAA GATCGCGGCC GCCTCGTTCT TCGCCAAGAA CATGCTGCCG CTGCTGACCT CGACCCGGGC CGTCCTGGAG AACGTCGACA ACGACATCAT GGAGCTGGAC GAAGCCGCTT TC
Details for MyabA.00185.c.A1.GE34915
HARVESTED ON: 4/17/2012
SEQUENCED ON: 4/17/2012
EXPECTED MW: 69kDa
OBSERVED MW: 69kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 43
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTHMGHYKS NVRDLEFNLF ELFKIQQVFG GEEFPELDED TARTFLAEMR TLAEGPLADS FAEGDRNPPV FDPETHSVAI PEAFKKSVKA ITEAGWDRVG LQEELGGTPV PRSLSWAIQE MILGANPAVW MYSGGAGFAQ IFYNIATDEQ KKWAEFVAER GWGATMVLTE PDAGSDVGAG RTKAVKQDDG SWHIDGVKRF ITSADSDDMF ENIMHLVLAR PEGAGPGTKG LSLFFVXKFI PNFXTGEPGE RNGVFVTNV
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacgc acatgggcca ctacaagtcg aacgtccgcg acctggagtt caacctcttc gagctcttca agatccagca ggtattcggc ggcgaggaat tccccgagct ggacgaagac accgctcgca ccttccttgc cgagatgcgc accctcgccg aagggccgct ggccgattca ttcgccgaag gcgaccgcaa cccgccggtt ttcgatccgg agacccactc ggtggccatc cccgaagcct tcaagaagtc cgtcaaggcc atcaccgagg ccggctggga ccgcgtcgga ctccaggaag agctcggcgg cacccccgtg ccgcggtcgc tgtcctgggc catccaagag atgatcctgg gcgccaaccc cgctgtctgg atgtacagcg gcggtgccgg tttcgcgcag atcttctaca acatcgccac cgatgagcag aagaagtggg ccgagttcgt cgccgagcgc ggctggggag ccaccatggt gctcaccgag cccgacgccg gttcggatgt gggcgccggc cgcaccaagg ccgtcaagca ggacgacggc tcatggcaca tcgacggtgt gaagcgcttc atcacctcgg ccgactcgga cgacatgttc gagaacatca tgcatctggt gctggctcgg cccgagggcg ccggcccggg caccaagggg ctgtcgctgt tcttcgtgnc gaagttcatc cccaacttcn aaaccggcga gcccggngag cgcaacggtg tgttcgtcac caacgtcna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTHMGHYKS NVRDLEFNLF ELFKIQQVFG GEEFPELDED TARTFLAEMR TLAEGPLADS FAEGDRNPPV FDPETHSVAI PEAFKKSVKA ITEAGWDRVG LQEELGGTPV PRSLSWAIQE MILGANPAVW MYSGGAGFAQ IFYNIATDEQ KKWAEFVAER GWGATMVLTE PDAGSDVGAG RTKAVKQDDG SWHIDGVKRF ITSADSDDMF ENIMHLVLAR PEGAGPGTKG LSLFFVPKFI PNFETGEPGE RNGVFVTNVE HKMGLKVSAT CELSFGQHGV PAKGWLVGEV HNGIAQMFDV IEQARMMVGT KAIATLSTGY LNALEYAKTR VQGADMTQLT DKTAPRVTIT HHPDVRRSLM TQKVYAEGLR ALYLYATTFQ DAVAAKTING VEAEDAVKLN DLLLPVIKGV GSERAYEKLT ESLQTFGGSG FLQDYPIEQY IRDAKIDSLY EGTTAIQAQD FFFRKIVKDQ GKSLAFISGQ IEEFVKSETG NGRLKAERAL LATALEDVQG MAASMTGNLM AAQQEITQVY RVGTASVRFL MSVGDLLIGW LLQRQAAVAI EALDAGATGA DKSFYEGKIA AASFFAKNML PLLTSTRAVL ENVDNDIMEL DEAAF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgcacatg ggccactaca agtcgaacgt ccgcgacctg gagttcaacc tcttcgagct cttcaagatc cagcaggtat tcggcggcga ggaattcccc gagctggacg aagacaccgc tcgcaccttc cttgccgaga tgcgcaccct cgccgaaggg ccgctggccg attcattcgc cgaaggcgac cgcaacccgc cggttttcga tccggagacc cactcggtgg ccatccccga agccttcaag aagtccgtca aggccatcac cgaggccggc tgggaccgcg tcggactcca ggaagagctc ggcggcaccc ccgtgccgcg gtcgctgtcc tgggccatcc aagagatgat cctgggcgcc aaccccgctg tctggatgta cagcggcggt gccggtttcg cgcagatctt ctacaacatc gccaccgatg agcagaagaa gtgggccgag ttcgtcgccg agcgcggctg gggagccacc atggtgctca ccgagcccga cgccggttcg gatgtgggcg ccggccgcac caaggccgtc aagcaggacg acggctcatg gcacatcgac ggtgtgaagc gcttcatcac ctcggccgac tcggacgaca tgttcgagaa catcatgcat ctggtgctgg ctcggcccga gggcgccggc ccgggcacca aggggctgtc gctgttcttc gtgccgaagt tcatccccaa cttcgaaacc ggcgagcccg gcgagcgcaa cggtgtgttc gtcaccaacg tcgagcacaa gatgggcctg aaggtttcgg ctacctgtga gctgagcttc ggtcagcacg gcgtacccgc caagggctgg ctggtcggcg aggtacacaa cggtattgcg cagatgttcg atgtcatcga gcaggcccgc atgatggtgg gcaccaaggc catcgccacg ctgtcgaccg gctacctgaa cgcgctggaa tacgccaaga cccgcgtgca gggtgcggac atgacgcagc tgaccgacaa gaccgctccc cgggtcacca tcacgcacca cccggacgtg cgtcgctcgc tgatgactca gaaggtttac gccgagggtc tgcgtgcgct gtacctctac gccaccacct tccaggatgc cgtggcggcc aagaccatca acggtgtcga ggccgaggac gcggtcaagc tcaacgacct gctgctgccg gtcatcaagg gtgtgggctc cgagcgcgcc tacgagaagc tcaccgagag cctgcagacc ttcggtggct ccggcttcct gcaggactac ccgatcgagc agtacatccg cgatgccaag atcgactcgc tgtacgaggg caccacggca atccaggccc aggacttctt cttccggaag atcgtcaagg accagggcaa gtcgctggcc ttcatttccg gccagatcga ggagttcgtc aagagcgaga ccggaaacgg ccgcctcaag gccgagcgtg cgctgctcgc gacggccctg gaggacgtgc agggtatggc ggcaagcatg actggcaacc tgatggctgc ccagcaggag atcacccagg tctacagggt cgggaccgcc tccgtgcgct tcctgatgag cgtgggcgac ctgctgatcg ggtggctgct gcagcgtcag gccgccgtgg ccatcgaggc tctcgatgcc ggtgccaccg gtgccgacaa gtccttctac gagggcaaga tcgcggccgc ctcgttcttc gccaagaaca tgctgccgct gctgacctcg acccgggccg tcctggagaa cgtcgacaac gacatcatgg agctggacga agccgctttc aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for MyabA.00185.c.A1.PW35530
PURIFICATION DATe: 6/14/2012
CONCENTRATION: 37mg/ml
OBSERVED MW: 66kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 11
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 43
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTHMGHYKS NVRDLEFNLF ELFKIQQVFG GEEFPELDED TARTFLAEMR TLAEGPLADS FAEGDRNPPV FDPETHSVAI PEAFKKSVKA ITEAGWDRVG LQEELGGTPV PRSLSWAIQE MILGANPAVW MYSGGAGFAQ IFYNIATDEQ KKWAEFVAER GWGATMVLTE PDAGSDVGAG RTKAVKQDDG SWHIDGVKRF ITSADSDDMF ENIMHLVLAR PEGAGPGTKG LSLFFVXKFI PNFXTGEPGE RNGVFVTNV
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacgc acatgggcca ctacaagtcg aacgtccgcg acctggagtt caacctcttc gagctcttca agatccagca ggtattcggc ggcgaggaat tccccgagct ggacgaagac accgctcgca ccttccttgc cgagatgcgc accctcgccg aagggccgct ggccgattca ttcgccgaag gcgaccgcaa cccgccggtt ttcgatccgg agacccactc ggtggccatc cccgaagcct tcaagaagtc cgtcaaggcc atcaccgagg ccggctggga ccgcgtcgga ctccaggaag agctcggcgg cacccccgtg ccgcggtcgc tgtcctgggc catccaagag atgatcctgg gcgccaaccc cgctgtctgg atgtacagcg gcggtgccgg tttcgcgcag atcttctaca acatcgccac cgatgagcag aagaagtggg ccgagttcgt cgccgagcgc ggctggggag ccaccatggt gctcaccgag cccgacgccg gttcggatgt gggcgccggc cgcaccaagg ccgtcaagca ggacgacggc tcatggcaca tcgacggtgt gaagcgcttc atcacctcgg ccgactcgga cgacatgttc gagaacatca tgcatctggt gctggctcgg cccgagggcg ccggcccggg caccaagggg ctgtcgctgt tcttcgtgnc gaagttcatc cccaacttcn aaaccggcga gcccggngag cgcaacggtg tgttcgtcac caacgtcna
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTHMGHYKS NVRDLEFNLF ELFKIQQVFG GEEFPELDED TARTFLAEMR TLAEGPLADS FAEGDRNPPV FDPETHSVAI PEAFKKSVKA ITEAGWDRVG LQEELGGTPV PRSLSWAIQE MILGANPAVW MYSGGAGFAQ IFYNIATDEQ KKWAEFVAER GWGATMVLTE PDAGSDVGAG RTKAVKQDDG SWHIDGVKRF ITSADSDDMF ENIMHLVLAR PEGAGPGTKG LSLFFVPKFI PNFETGEPGE RNGVFVTNVE HKMGLKVSAT CELSFGQHGV PAKGWLVGEV HNGIAQMFDV IEQARMMVGT KAIATLSTGY LNALEYAKTR VQGADMTQLT DKTAPRVTIT HHPDVRRSLM TQKVYAEGLR ALYLYATTFQ DAVAAKTING VEAEDAVKLN DLLLPVIKGV GSERAYEKLT ESLQTFGGSG FLQDYPIEQY IRDAKIDSLY EGTTAIQAQD FFFRKIVKDQ GKSLAFISGQ IEEFVKSETG NGRLKAERAL LATALEDVQG MAASMTGNLM AAQQEITQVY RVGTASVRFL MSVGDLLIGW LLQRQAAVAI EALDAGATGA DKSFYEGKIA AASFFAKNML PLLTSTRAVL ENVDNDIMEL DEAAF
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACGCACATG GGCCACTACA AGTCGAACGT CCGCGACCTG GAGTTCAACC TCTTCGAGCT CTTCAAGATC CAGCAGGTAT TCGGCGGCGA GGAATTCCCC GAGCTGGACG AAGACACCGC TCGCACCTTC CTTGCCGAGA TGCGCACCCT CGCCGAAGGG CCGCTGGCCG ATTCATTCGC CGAAGGCGAC CGCAACCCGC CGGTTTTCGA TCCGGAGACC CACTCGGTGG CCATCCCCGA AGCCTTCAAG AAGTCCGTCA AGGCCATCAC CGAGGCCGGC TGGGACCGCG TCGGACTCCA GGAAGAGCTC GGCGGCACCC CCGTGCCGCG GTCGCTGTCC TGGGCCATCC AAGAGATGAT CCTGGGCGCC AACCCCGCTG TCTGGATGTA CAGCGGCGGT GCCGGTTTCG CGCAGATCTT CTACAACATC GCCACCGATG AGCAGAAGAA GTGGGCCGAG TTCGTCGCCG AGCGCGGCTG GGGAGCCACC ATGGTGCTCA CCGAGCCCGA CGCCGGTTCG GATGTGGGCG CCGGCCGCAC CAAGGCCGTC AAGCAGGACG ACGGCTCATG GCACATCGAC GGTGTGAAGC GCTTCATCAC CTCGGCCGAC TCGGACGACA TGTTCGAGAA CATCATGCAT CTGGTGCTGG CTCGGCCCGA GGGCGCCGGC CCGGGCACCA AGGGGCTGTC GCTGTTCTTC GTGCCGAAGT TCATCCCCAA CTTCGAAACC GGCGAGCCCG GCGAGCGCAA CGGTGTGTTC GTCACCAACG TCGAGCACAA GATGGGCCTG AAGGTTTCGG CTACCTGTGA GCTGAGCTTC GGTCAGCACG GCGTACCCGC CAAGGGCTGG CTGGTCGGCG AGGTACACAA CGGTATTGCG CAGATGTTCG ATGTCATCGA GCAGGCCCGC ATGATGGTGG GCACCAAGGC CATCGCCACG CTGTCGACCG GCTACCTGAA CGCGCTGGAA TACGCCAAGA CCCGCGTGCA GGGTGCGGAC ATGACGCAGC TGACCGACAA GACCGCTCCC CGGGTCACCA TCACGCACCA CCCGGACGTG CGTCGCTCGC TGATGACTCA GAAGGTTTAC GCCGAGGGTC TGCGTGCGCT GTACCTCTAC GCCACCACCT TCCAGGATGC CGTGGCGGCC AAGACCATCA ACGGTGTCGA GGCCGAGGAC GCGGTCAAGC TCAACGACCT GCTGCTGCCG GTCATCAAGG GTGTGGGCTC CGAGCGCGCC TACGAGAAGC TCACCGAGAG CCTGCAGACC TTCGGTGGCT CCGGCTTCCT GCAGGACTAC CCGATCGAGC AGTACATCCG CGATGCCAAG ATCGACTCGC TGTACGAGGG CACCACGGCA ATCCAGGCCC AGGACTTCTT CTTCCGGAAG ATCGTCAAGG ACCAGGGCAA GTCGCTGGCC TTCATTTCCG GCCAGATCGA GGAGTTCGTC AAGAGCGAGA CCGGAAACGG CCGCCTCAAG GCCGAGCGTG CGCTGCTCGC GACGGCCCTG GAGGACGTGC AGGGTATGGC GGCAAGCATG ACTGGCAACC TGATGGCTGC CCAGCAGGAG ATCACCCAGG TCTACAGGGT CGGGACCGCC TCCGTGCGCT TCCTGATGAG CGTGGGCGAC CTGCTGATCG GGTGGCTGCT GCAGCGTCAG GCCGCCGTGG CCATCGAGGC TCTCGATGCC GGTGCCACCG GTGCCGACAA GTCCTTCTAC GAGGGCAAGA TCGCGGCCGC CTCGTTCTTC GCCAAGAACA TGCTGCCGCT GCTGACCTCG ACCCGGGCCG TCCTGGAGAA CGTCGACAAC GACATCATGG AGCTGGACGA AGCCGCTTTC AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA