MyabA.00185.a

Putative acyl-CoA dehydrogenase

CENTER ID: MyabA.00185.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00185.a.A1.GE30261 Full length( MyabA.00185.a ) 1 400
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00185.a.A1.PS01159 Full length( MyabA.00185.a ) 1 400

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.3175
RefSeq: YP_001703854.1
UniProt: B1MD79

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSASEDHQTR MSASEDNQTR MSASEDNQAQ INEFEFTEEH GDLRSLVRSW CERVWTPKHV RQIAEAGSVD LDAWRALGSE LGVVGLSLPE QHGGGGLGVI ELSIVAEELG RALACLPWIS SAALGATALV SSGDDSALAE WVPALAAGEK TITLAGGRTR LADAITVSAE SDTDGWKLTG DTEHVPDGAT ADVILVLADT DSGPSLFAVD GDAPGVDRHS LATLDMTRRQ ANIHFNSAPA RLIGAQGRGA EAVATALDVA ATVLAAEQAG IAAHMLDVTT EYAKSRIQFG RIIGSFQAVK HRLADMAAAT GNVRGAAYHA AWSHDHPSLD DPALATSIAQ QVASAGAVEV TAKAIQSHGG IGFTWEHPAH LYYKRAVSNS ALFGGRAVHA ERIAKEVIDA
NT Sequence
ATGAGCGCCA GCGAAGACCA TCAAACCCGG ATGAGCGCCA GCGAAGACAA TCAAACCCGG ATGAGCGCCA GCGAAGACAA TCAGGCCCAG ATCAATGAAT TCGAGTTCAC CGAAGAACAC GGCGACCTGC GCTCGCTGGT ACGCAGCTGG TGTGAACGGG TCTGGACACC CAAGCACGTG CGCCAGATTG CCGAGGCTGG ATCAGTGGAT CTGGATGCCT GGCGCGCCCT CGGCTCCGAA CTGGGTGTGG TGGGCCTAAG TCTGCCCGAA CAGCATGGCG GCGGTGGGTT GGGTGTCATC GAATTGTCCA TCGTGGCCGA GGAATTGGGG CGGGCTCTGG CGTGCCTGCC GTGGATATCC AGTGCTGCCC TGGGCGCCAC CGCCCTGGTG TCCAGCGGCG ACGACAGCGC CTTGGCCGAG TGGGTACCGG CGCTGGCCGC CGGCGAGAAG ACGATCACGC TGGCGGGCGG CCGTACCCGT TTAGCGGACG CGATCACCGT GTCTGCCGAA TCCGATACCG ACGGCTGGAA GCTCACCGGC GACACCGAGC ATGTTCCCGA CGGCGCGACC GCCGACGTGA TCCTGGTGCT CGCCGATACC GATTCGGGAC CAAGCCTTTT CGCGGTCGAC GGCGATGCGC CAGGTGTCGA CCGACACTCG CTGGCGACCC TTGATATGAC CCGGCGGCAG GCCAACATCC ACTTCAACTC CGCCCCCGCA CGCCTGATCG GTGCACAGGG GCGGGGTGCG GAGGCCGTCG CCACGGCACT CGACGTCGCA GCCACCGTAC TGGCGGCCGA GCAGGCCGGT ATCGCCGCCC ACATGCTGGA TGTGACAACC GAATACGCGA AGTCCCGGAT CCAGTTCGGG CGCATCATCG GGTCATTCCA GGCCGTCAAA CACCGCCTTG CCGATATGGC CGCGGCGACC GGGAATGTGC GTGGCGCCGC GTATCACGCG GCCTGGTCAC ATGATCACCC GAGCCTTGAC GATCCGGCGC TGGCCACCAG TATTGCTCAA CAGGTGGCGT CGGCAGGAGC TGTGGAGGTA ACCGCCAAGG CCATCCAGAG CCATGGCGGC ATCGGTTTCA CCTGGGAGCA CCCGGCCCAT CTGTATTACA AACGCGCCGT GAGTAACTCG GCCCTCTTCG GGGGGCGCGC CGTCCACGCC GAACGTATTG CCAAGGAGGT AATCGACGCA
Details for MyabA.00185.a.A1.GE30261
HARVESTED ON: 8/31/2010
SEQUENCED ON: 9/7/2010
EXPECTED MW: 44kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 20
PERCENT COVERAGE: 58
Validated AA Sequence
MMRPNWIRDF AYSVVTSSMW AAIPACSAAS TVAATSSAVA TASAPRPCAP IRRAGAELKW MLACRRVISR VASECRSTPG ASPSTAKRLG PESVSASTRI TSAVAPSGTC SVSPVSFQPS VSDSADTVIA SAKRVRPPAS VIVFSPAASA GTHSAKALSS PLDTRAVAPR AALDIHGRHA RARPNSSATM DNSMTPNPPP PCCSGRLRPT TPSSEPRARQ ASRSTDPASA IWRTCLGVQT RSHQLRT
Validated NT Sequence
cgattacctc cttggcaata cgttcggcgt ggacggcgcg ccccccgaag agggccgagt tactcacggc gcgtttgtaa tacagatggg ccgggtgctc ccaggtgaaa ccgatgccgc catggctctg gatggccttg gcggttacct ccacagctcc tgccgacgcc acctgttgag caatactggt ggccagcgcc ggatcgtcaa ggctcgggtg atcatgtgac caggccgcgt gatacgcggc gccacgcaca ttcccggtcg ccgcggccat atcggcaagg cggtgtttga cggcctggaa tgacccgatg atgcgcccga actggatccg ggacttcgcg tattcggttg tcacatccag catgtgggcg gcgataccgg cctgctcggc cgccagtacg gtggctgcga cgtcgagtgc cgtggcgacg gcctccgcac cccgcccctg tgcaccgatc aggcgtgcgg gggcggagtt gaagtggatg ttggcctgcc gccgggtcat atcaagggtc gccagcgagt gtcggtcgac acctggcgca tcgccgtcga ccgcgaaaag gcttggtccc gaatcggtat cggcgagcac caggatcacg tcggcggtcg cgccgtcggg aacatgctcg gtgtcgccgg tgagcttcca gccgtcggta tcggattcgg cagacacggt gatcgcgtcc gctaaacggg tacggccgcc cgccagcgtg atcgtcttct cgccggcggc cagcgccggt acccactcgg ccaaggcgct gtcgtcgccg ctggacacca gggcggtggc gcccagggca gcactggata tccacggcag gcacgccaga gcccgcccca attcctcggc cacgatggac aattcgatga cacccaaccc accgccgcca tgctgttcgg gcagacttag gcccaccaca cccagttcgg agccgagggc gcgccaggca tccagatcca ctgatccagc ctcggcaatc tggcgcacgt gcttgggtgt ccagacccgt tcacaccagc tgcgtacca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSASEDHQT RMSASEDNQT RMSASEDNQA QINEFEFTEE HGDLRSLVRS WCERVWTPKH VRQIAEAGSV DLDAWRALGS ELGVVGLSLP EQHGGGGLGV IELSIVAEEL GRALACLPWI SSAALGATAL VSSGDDSALA EWVPALAAGE KTITLAGGRT RLADAITVSA ESDTDGWKLT GDTEHVPDGA TADVILVLAD TDSGPSLFAV DGDAPGVDRH SLATLDMTRR QANIHFNSAP ARLIGAQGRG AEAVATALDV AATVLAAEQA GIAAHMLDVT TEYAKSRIQF GRIIGSFQAV KHRLADMAAA TGNVRGAAYH AAWSHDHPSL DDPALATSIA QQVASAGAVE VTAKAIQSHG GIGFTWEHPA HLYYKRAVSN SALFGGRAVH AERIAKEVID A
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgccagc gaagaccatc aaacccggat gagcgccagc gaagacaatc aaacccggat gagcgccagc gaagacaatc aggcccagat caatgaattc gagttcaccg aagaacacgg cgacctgcgc tcgctggtac gcagctggtg tgaacgggtc tggacaccca agcacgtgcg ccagattgcc gaggctggat cagtggatct ggatgcctgg cgcgccctcg gctccgaact gggtgtggtg ggcctaagtc tgcccgaaca gcatggcggc ggtgggttgg gtgtcatcga attgtccatc gtggccgagg aattggggcg ggctctggcg tgcctgccgt ggatatccag tgctgccctg ggcgccaccg ccctggtgtc cagcggcgac gacagcgcct tggccgagtg ggtaccggcg ctggccgccg gcgagaagac gatcacgctg gcgggcggcc gtacccgttt agcggacgcg atcaccgtgt ctgccgaatc cgataccgac ggctggaagc tcaccggcga caccgagcat gttcccgacg gcgcgaccgc cgacgtgatc ctggtgctcg ccgataccga ttcgggacca agccttttcg cggtcgacgg cgatgcgcca ggtgtcgacc gacactcgct ggcgaccctt gatatgaccc ggcggcaggc caacatccac ttcaactccg cccccgcacg cctgatcggt gcacaggggc ggggtgcgga ggccgtcgcc acggcactcg acgtcgcagc caccgtactg gcggccgagc aggccggtat cgccgcccac atgctggatg tgacaaccga atacgcgaag tcccggatcc agttcgggcg catcatcggg tcattccagg ccgtcaaaca ccgccttgcc gatatggccg cggcgaccgg gaatgtgcgt ggcgccgcgt atcacgcggc ctggtcacat gatcacccga gccttgacga tccggcgctg gccaccagta ttgctcaaca ggtggcgtcg gcaggagctg tggaggtaac cgccaaggcc atccagagcc atggcggcat cggtttcacc tgggagcacc cggcccatct gtattacaaa cgcgccgtga gtaactcggc cctcttcggg gggcgcgccg tccacgccga acgtattgcc aaggaggtaa tcgacgcaaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for MyabA.00185.a.A1.PS01159
PURIFICATION DATe: 7/20/2011
CONCENTRATION: 30.2mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 100µl
PERCENT IDENTITY: 20
PERCENT COVERAGE: 58
Protocol Notes
notes unavailable
Validated AA Sequence
MMRPNWIRDF AYSVVTSSMW AAIPACSAAS TVAATSSAVA TASAPRPCAP IRRAGAELKW MLACRRVISR VASECRSTPG ASPSTAKRLG PESVSASTRI TSAVAPSGTC SVSPVSFQPS VSDSADTVIA SAKRVRPPAS VIVFSPAASA GTHSAKALSS PLDTRAVAPR AALDIHGRHA RARPNSSATM DNSMTPNPPP PCCSGRLRPT TPSSEPRARQ ASRSTDPASA IWRTCLGVQT RSHQLRT
Validated NT Sequence
cgattacctc cttggcaata cgttcggcgt ggacggcgcg ccccccgaag agggccgagt tactcacggc gcgtttgtaa tacagatggg ccgggtgctc ccaggtgaaa ccgatgccgc catggctctg gatggccttg gcggttacct ccacagctcc tgccgacgcc acctgttgag caatactggt ggccagcgcc ggatcgtcaa ggctcgggtg atcatgtgac caggccgcgt gatacgcggc gccacgcaca ttcccggtcg ccgcggccat atcggcaagg cggtgtttga cggcctggaa tgacccgatg atgcgcccga actggatccg ggacttcgcg tattcggttg tcacatccag catgtgggcg gcgataccgg cctgctcggc cgccagtacg gtggctgcga cgtcgagtgc cgtggcgacg gcctccgcac cccgcccctg tgcaccgatc aggcgtgcgg gggcggagtt gaagtggatg ttggcctgcc gccgggtcat atcaagggtc gccagcgagt gtcggtcgac acctggcgca tcgccgtcga ccgcgaaaag gcttggtccc gaatcggtat cggcgagcac caggatcacg tcggcggtcg cgccgtcggg aacatgctcg gtgtcgccgg tgagcttcca gccgtcggta tcggattcgg cagacacggt gatcgcgtcc gctaaacggg tacggccgcc cgccagcgtg atcgtcttct cgccggcggc cagcgccggt acccactcgg ccaaggcgct gtcgtcgccg ctggacacca gggcggtggc gcccagggca gcactggata tccacggcag gcacgccaga gcccgcccca attcctcggc cacgatggac aattcgatga cacccaaccc accgccgcca tgctgttcgg gcagacttag gcccaccaca cccagttcgg agccgagggc gcgccaggca tccagatcca ctgatccagc ctcggcaatc tggcgcacgt gcttgggtgt ccagacccgt tcacaccagc tgcgtacca
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSASEDHQT RMSASEDNQT RMSASEDNQA QINEFEFTEE HGDLRSLVRS WCERVWTPKH VRQIAEAGSV DLDAWRALGS ELGVVGLSLP EQHGGGGLGV IELSIVAEEL GRALACLPWI SSAALGATAL VSSGDDSALA EWVPALAAGE KTITLAGGRT RLADAITVSA ESDTDGWKLT GDTEHVPDGA TADVILVLAD TDSGPSLFAV DGDAPGVDRH SLATLDMTRR QANIHFNSAP ARLIGAQGRG AEAVATALDV AATVLAAEQA GIAAHMLDVT TEYAKSRIQF GRIIGSFQAV KHRLADMAAA TGNVRGAAYH AAWSHDHPSL DDPALATSIA QQVASAGAVE VTAKAIQSHG GIGFTWEHPA HLYYKRAVSN SALFGGRAVH AERIAKEVID A
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCGCCAGC GAAGACCATC AAACCCGGAT GAGCGCCAGC GAAGACAATC AAACCCGGAT GAGCGCCAGC GAAGACAATC AGGCCCAGAT CAATGAATTC GAGTTCACCG AAGAACACGG CGACCTGCGC TCGCTGGTAC GCAGCTGGTG TGAACGGGTC TGGACACCCA AGCACGTGCG CCAGATTGCC GAGGCTGGAT CAGTGGATCT GGATGCCTGG CGCGCCCTCG GCTCCGAACT GGGTGTGGTG GGCCTAAGTC TGCCCGAACA GCATGGCGGC GGTGGGTTGG GTGTCATCGA ATTGTCCATC GTGGCCGAGG AATTGGGGCG GGCTCTGGCG TGCCTGCCGT GGATATCCAG TGCTGCCCTG GGCGCCACCG CCCTGGTGTC CAGCGGCGAC GACAGCGCCT TGGCCGAGTG GGTACCGGCG CTGGCCGCCG GCGAGAAGAC GATCACGCTG GCGGGCGGCC GTACCCGTTT AGCGGACGCG ATCACCGTGT CTGCCGAATC CGATACCGAC GGCTGGAAGC TCACCGGCGA CACCGAGCAT GTTCCCGACG GCGCGACCGC CGACGTGATC CTGGTGCTCG CCGATACCGA TTCGGGACCA AGCCTTTTCG CGGTCGACGG CGATGCGCCA GGTGTCGACC GACACTCGCT GGCGACCCTT GATATGACCC GGCGGCAGGC CAACATCCAC TTCAACTCCG CCCCCGCACG CCTGATCGGT GCACAGGGGC GGGGTGCGGA GGCCGTCGCC ACGGCACTCG ACGTCGCAGC CACCGTACTG GCGGCCGAGC AGGCCGGTAT CGCCGCCCAC ATGCTGGATG TGACAACCGA ATACGCGAAG TCCCGGATCC AGTTCGGGCG CATCATCGGG TCATTCCAGG CCGTCAAACA CCGCCTTGCC GATATGGCCG CGGCGACCGG GAATGTGCGT GGCGCCGCGT ATCACGCGGC CTGGTCACAT GATCACCCGA GCCTTGACGA TCCGGCGCTG GCCACCAGTA TTGCTCAACA GGTGGCGTCG GCAGGAGCTG TGGAGGTAAC CGCCAAGGCC ATCCAGAGCC ATGGCGGCAT CGGTTTCACC TGGGAGCACC CGGCCCATCT GTATTACAAA CGCGCCGTGA GTAACTCGGC CCTCTTCGGG GGGCGCGCCG TCCACGCCGA ACGTATTGCC AAGGAGGTAA TCGACGCAAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA