MyabA.00170.a
Enoyl-[acyl-carrier-protein] reductase [NADH] (EC 1.3.1.9) (InhA)
| CENTER ID: | MyabA.00170.a |
| ORGANISM: | Mycobacterium abscessus ATCC 19977 / DSM 44196 |
| ASSOCIATED DISEASE: | Skin abscess |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyabA.00170.a.B1.GE42830 | full length | 1 | 269 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyabA.00170.a.B1.PS38577 | full length | 1 | 269 |
Structures
7KLI
DEPOSITED: 10/30/2020
DETERMINATION: XRay
CLONE: MyabA.00170.a.B1.GE42830
PROTEIN: MyabA.00170.a.B1.PS38577
7L6C
DEPOSITED: 12/23/2020
DETERMINATION: XRay
CLONE: MyabA.00170.a.B1.GE42830
PROTEIN: MyabA.00170.a.B1.PS38577
7U0M
DEPOSITED: 2/18/2022
DETERMINATION: XRay
CLONE: MyabA.00170.a.B1.GE42830
PROTEIN: MyabA.00170.a.B1.PS38577
Publications by SSGCID
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| UniProt: | B1MC30 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAGLLEGKRI LVTGIITDSS IAFHIAKVAQ EQGAELVLTG FDRLRLIERI TQRLPKPAPL LELDVQNEEH LGSLAGRISE VIGEGNKLDG VVHSIGFMPQ SGMGVNPFFD APFADVSKGF HISAFSYSSL AKAVLPVMNR GGSIVGMDFD PTRAMPAYNW MTVAKSALES VNRFVAREAG KVGVRSNLVA AGPIRTLAMS AIVGGALGDE AGQQMQLLEE GWDQRAPIGW DMKDPTPVAK TVCALLSDWL PATTGDIIFA DGGAHTQLL
NT Sequence
gtggcaggac tacttgaggg caagcgcatc ctggtgacgg gcatcatcac cgattcgtcc atcgcattcc acatcgcgaa ggtcgcgcag gaacagggcg cagagctggt gctcaccgga ttcgaccggc tgcgcctgat cgagcgcatc acccagcgcc tacccaagcc ggccccgctg ctggagctcg acgtgcagaa cgaggagcac ctgggcagcc tggctgggcg catttccgag gtgatcggcg agggcaacaa gctcgatggt gttgtgcact cgatcggttt catgccgcag agcggaatgg gcgtcaaccc cttcttcgac gcgccgttcg cggacgtctc caagggcttc cacatttcgg cgttctcgta ctcgtcgctg gccaaggccg tgctaccggt catgaaccgg ggcggcagca ttgtgggtat ggacttcgac ccgactcggg cgatgcctgc ctacaactgg atgaccgtgg ccaagagcgc gctggagtcg gtgaaccgat tcgtcgcccg tgaggcaggc aaggtcggtg tccgttccaa tctggtggcg gccggaccga tccgcacgtt ggccatgagc gccattgtgg gcggtgccct cggcgatgag gccggtcagc aaatgcagct gctggaagag ggctgggatc agcgtgcccc gatcggctgg gacatgaagg acccgacacc cgtggccaag acggtgtgcg cgctgctgtc cgactggttg cccgccacca ccggcgacat cattttcgcc gacggcggcg ctcacactca gctgctctga
Details for MyabA.00170.a.B1.GE42830
| HARVESTED ON: | 1/9/2019 |
| SEQUENCED ON: | 1/15/2019 |
| EXPECTED MW: | 30kDa |
| OBSERVED MW: | 30kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 97 |
| PERCENT COVERAGE: | 101 |
Validated AA Sequence
MAHHHHHHMV AGLLEGKRIL VTGIITDSSI AFHIAKVAQE QGAELVLTGF DRLRLIERIT QRLPKPAPLL ELDVQNEEHL GSLAGRISEV IGEGNKLDGV VHSIGFMPQS GMGVNPFFDA PFADVSKGFH ISAFSYSSLA KAVLPVMNRG GSIVGMDFDP TRAMPAYNWM TVAKSALESV NRFVAREAGK VGVRSNLVAA GPIRTLAMSA IVGGALGDEA GQQMQLLEEG WDQRAPIGWD MKDPTPVAKT VCALLSDWLP ATTGDIIFAX XXXSHSAALS E
Validated NT Sequence
ttactcactt agagcagctg agtgtgagnn nnnnnnntcg gcgaaaatga tgtcgccggt ggtggcgggc aaccagtcgg acagcagcgc gcacaccgtc ttggccacgg gtgtcgggtc cttcatgtcc cagccgatcg gggcacgctg atcccagccc tcttccagca gctgcatttg ctgaccggcc tcatcgccga gggcaccgcc cacaatggcg ctcatggcca acgtgcggat cggtccggcc gccaccagat tggaacggac accgaccttg cctgcctcac gggcgacgaa tcggttcacc gactccagcg cgctcttggc cacggtcatc cagttgtagg caggcatcgc ccgagtcggg tcgaagtcca tacccacaat gctgccgccc cggttcatga ccggtagcac ggccttggcc agcgacgagt acgagaacgc cgaaatgtgg aagcccttgg agacgtccgc gaacggcgcg tcgaagaagg ggttgacgcc cattccgctc tgcggcatga aaccgatcga gtgcacaaca ccatcgagct tgttgccctc gccgatcacc tcggaaatgc gcccagccag gctgcccagg tgctcctcgt tctgcacgtc gagctccagc agcggggccg gcttgggtag gcgctgggtg atgcgctcga tcaggcgcag ccggtcgaat ccggtgagca ccagctctgc gccctgttcc tgcgcgacct tcgcgatgtg gaatgcgatg gacgaatcgg tgatgatgcc cgtcaccagg atgcgcttgc cctcaagtag tcctgccacc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHMA GLLEGKRILV TGIITDSSIA FHIAKVAQEQ GAELVLTGFD RLRLIERITQ RLPKPAPLLE LDVQNEEHLG SLAGRISEVI GEGNKLDGVV HSIGFMPQSG MGVNPFFDAP FADVSKGFHI SAFSYSSLAK AVLPVMNRGG SIVGMDFDPT RAMPAYNWMT VAKSALESVN RFVAREAGKV GVRSNLVAAG PIRTLAMSAI VGGALGDEAG QQMQLLEEGW DQRAPIGWDM KDPTPVAKTV CALLSDWLPA TTGDIIFADG GAHTQLL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtggcag gactacttga gggcaagcgc atcctggtga cgggcatcat caccgattcg tccatcgcat tccacatcgc gaaggtcgcg caggaacagg gcgcagagct ggtgctcacc ggattcgacc ggctgcgcct gatcgagcgc atcacccagc gcctacccaa gccggccccg ctgctggagc tcgacgtgca gaacgaggag cacctgggca gcctggctgg gcgcatttcc gaggtgatcg gcgagggcaa caagctcgat ggtgttgtgc actcgatcgg tttcatgccg cagagcggaa tgggcgtcaa ccccttcttc gacgcgccgt tcgcggacgt ctccaagggc ttccacattt cggcgttctc gtactcgtcg ctggccaagg ccgtgctacc ggtcatgaac cggggcggca gcattgtggg tatggacttc gacccgactc gggcgatgcc tgcctacaac tggatgaccg tggccaagag cgcgctggag tcggtgaacc gattcgtcgc ccgtgaggca ggcaaggtcg gtgtccgttc caatctggtg gcggccggac cgatccgcac gttggccatg agcgccattg tgggcggtgc cctcggcgat gaggccggtc agcaaatgca gctgctggaa gagggctggg atcagcgtgc cccgatcggc tgggacatga aggacccgac acccgtggcc aagacggtgt gcgcgctgct gtccgactgg ttgcccgcca ccaccggcga catcattttc gccgacggcg gcgctcacac tcagctgctc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for MyabA.00170.a.B1.PS38577
| PURIFICATION DATe: | 5/7/2019 |
| CONCENTRATION: | 23.06mg/ml |
| OBSERVED MW: | 30kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 97 |
| PERCENT COVERAGE: | 101 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMV AGLLEGKRIL VTGIITDSSI AFHIAKVAQE QGAELVLTGF DRLRLIERIT QRLPKPAPLL ELDVQNEEHL GSLAGRISEV IGEGNKLDGV VHSIGFMPQS GMGVNPFFDA PFADVSKGFH ISAFSYSSLA KAVLPVMNRG GSIVGMDFDP TRAMPAYNWM TVAKSALESV NRFVAREAGK VGVRSNLVAA GPIRTLAMSA IVGGALGDEA GQQMQLLEEG WDQRAPIGWD MKDPTPVAKT VCALLSDWLP ATTGDIIFAX XXXSHSAALS E
Validated NT Sequence
ttactcactt agagcagctg agtgtgagnn nnnnnnntcg gcgaaaatga tgtcgccggt ggtggcgggc aaccagtcgg acagcagcgc gcacaccgtc ttggccacgg gtgtcgggtc cttcatgtcc cagccgatcg gggcacgctg atcccagccc tcttccagca gctgcatttg ctgaccggcc tcatcgccga gggcaccgcc cacaatggcg ctcatggcca acgtgcggat cggtccggcc gccaccagat tggaacggac accgaccttg cctgcctcac gggcgacgaa tcggttcacc gactccagcg cgctcttggc cacggtcatc cagttgtagg caggcatcgc ccgagtcggg tcgaagtcca tacccacaat gctgccgccc cggttcatga ccggtagcac ggccttggcc agcgacgagt acgagaacgc cgaaatgtgg aagcccttgg agacgtccgc gaacggcgcg tcgaagaagg ggttgacgcc cattccgctc tgcggcatga aaccgatcga gtgcacaaca ccatcgagct tgttgccctc gccgatcacc tcggaaatgc gcccagccag gctgcccagg tgctcctcgt tctgcacgtc gagctccagc agcggggccg gcttgggtag gcgctgggtg atgcgctcga tcaggcgcag ccggtcgaat ccggtgagca ccagctctgc gccctgttcc tgcgcgacct tcgcgatgtg gaatgcgatg gacgaatcgg tgatgatgcc cgtcaccagg atgcgcttgc cctcaagtag tcctgccacc atatggtggt ggtggtggtg agccat
Expressed Protein Sequence
MAHHHHHHMA GLLEGKRILV TGIITDSSIA FHIAKVAQEQ GAELVLTGFD RLRLIERITQ RLPKPAPLLE LDVQNEEHLG SLAGRISEVI GEGNKLDGVV HSIGFMPQSG MGVNPFFDAP FADVSKGFHI SAFSYSSLAK AVLPVMNRGG SIVGMDFDPT RAMPAYNWMT VAKSALESVN RFVAREAGKV GVRSNLVAAG PIRTLAMSAI VGGALGDEAG QQMQLLEEGW DQRAPIGWDM KDPTPVAKTV CALLSDWLPA TTGDIIFADG GAHTQLL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGGCAG GACTACTTGA GGGCAAGCGC ATCCTGGTGA CGGGCATCAT CACCGATTCG TCCATCGCAT TCCACATCGC GAAGGTCGCG CAGGAACAGG GCGCAGAGCT GGTGCTCACC GGATTCGACC GGCTGCGCCT GATCGAGCGC ATCACCCAGC GCCTACCCAA GCCGGCCCCG CTGCTGGAGC TCGACGTGCA GAACGAGGAG CACCTGGGCA GCCTGGCTGG GCGCATTTCC GAGGTGATCG GCGAGGGCAA CAAGCTCGAT GGTGTTGTGC ACTCGATCGG TTTCATGCCG CAGAGCGGAA TGGGCGTCAA CCCCTTCTTC GACGCGCCGT TCGCGGACGT CTCCAAGGGC TTCCACATTT CGGCGTTCTC GTACTCGTCG CTGGCCAAGG CCGTGCTACC GGTCATGAAC CGGGGCGGCA GCATTGTGGG TATGGACTTC GACCCGACTC GGGCGATGCC TGCCTACAAC TGGATGACCG TGGCCAAGAG CGCGCTGGAG TCGGTGAACC GATTCGTCGC CCGTGAGGCA GGCAAGGTCG GTGTCCGTTC CAATCTGGTG GCGGCCGGAC CGATCCGCAC GTTGGCCATG AGCGCCATTG TGGGCGGTGC CCTCGGCGAT GAGGCCGGTC AGCAAATGCA GCTGCTGGAA GAGGGCTGGG ATCAGCGTGC CCCGATCGGC TGGGACATGA AGGACCCGAC ACCCGTGGCC AAGACGGTGT GCGCGCTGCT GTCCGACTGG TTGCCCGCCA CCACCGGCGA CATCATTTTC GCCGACGGCG GCGCTCACAC TCAGCTGCTC TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT