MyabA.00168.a

2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (IspD)

CENTER ID:	MyabA.00168.a
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.00168.a.A1.GE30260	Full length( MyabA.00168.a )		1	210

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_129829
PATRIC ID:	fig\|36809.5.peg.609
RefSeq:	YP_001701321.1
UniProt:	B1MGY3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRLGANTPKA FVSVGGRTML QRCVDGLLAS GAVDEVVVVV GADQLERAAA LVGSGATVVQ GGAERTDSVR AGLAAVDAAD WILVHDAARP LTPPDMIARI VAELRTGRGA VIPAVAVADT IKSIDVHGDV IGTPDRAGLR AVQTPQGFAA DVLRRAYAAA GDIATDDAAL VEQIGERVHV VEGDRLAFKI TTTLDMTLAE AILGATEGVQ

NT Sequence

GTGCGGCTGG GTGCAAATAC CCCGAAGGCC TTCGTGAGTG TCGGCGGCCG GACCATGCTT CAGCGTTGTG TGGACGGCCT GCTCGCATCC GGGGCCGTGG ACGAGGTAGT GGTCGTGGTC GGTGCCGATC AGCTCGAGAG GGCCGCTGCC CTGGTGGGCT CCGGGGCCAC CGTTGTGCAG GGCGGTGCCG AGCGCACCGA CTCGGTGCGG GCCGGTTTGG CCGCCGTGGA TGCGGCCGAC TGGATTCTGG TTCACGATGC CGCGCGTCCG TTGACCCCTC CGGACATGAT CGCCCGCATC GTCGCAGAGC TGCGTACGGG CCGCGGCGCT GTCATTCCGG CGGTTGCCGT CGCGGACACC ATCAAATCGA TCGACGTTCA CGGTGACGTG ATCGGCACTC CCGACCGGGC GGGCCTGCGC GCCGTACAGA CCCCACAGGG CTTCGCCGCC GACGTTCTCA GGCGTGCGTA CGCGGCCGCA GGTGATATCG CCACAGATGA TGCCGCACTG GTTGAGCAGA TCGGTGAGAG GGTTCACGTG GTGGAAGGCG ACCGGTTGGC CTTCAAAATC ACCACGACAC TCGACATGAC ATTGGCCGAG GCGATTCTCG GTGCTACGGA AGGCGTCCAA

Details for MyabA.00168.a.A1.GE30260

HARVESTED ON:	8/31/2010
SEQUENCED ON:	9/7/2010
EXPECTED MW:	23kDa
OBSERVED MW:	24kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVRLGANTP KAFVSVGGRT MLQRCVDGLL ASGAVDEVVV VVGADQLERA AALVGSGATV VQGGAERTDS VRAGLAAVDA ADWILVHDAA RPLTPPDMIA RIVAELRTGR GAVIPAVAVA DTIKSIDVHG DVIGTPDRAG LRAVQTPQGF AADVLRRAYA AAGDIATDDA ALVEQIGERV HVVEGDRLAF KITTTLDMTL AXAILGATEG VQ

Validated NT Sequence

cnnnnnnatt ttntttactt taagaaggag anataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atggtgcggc tgggtgcaaa taccccgaag gccttcgtga gtgtcggcgg ccggaccatg cttcagcgtt gtgtggacgg cctgctcgca tccggggccg tggacgaggt agtggtcgtg gtcggtgccg atcagctcga gagggccgct gccctggtgg gctccggggc caccgttgtg cagggcggtg ccgagcgcac cgactcggtg cgggccggtt tggccgccgt ggatgcggcc gactggattc tggttcacga tgccgcgcgt ccgttgaccc ctccggacat gatcgcccgc atcgtcgcag agctgcgtac gggccgcggc gctgtcattc cggcggttgc cgtcgcggac accatcaaat cgatcgacgt tcacggtgac gtgatcggca ctcccgaccg ggcgggcctg cgcgccgtac agaccccaca gggcttcgcc gccgacgttc tcaggcgtgc gtacgcggcc gcaggtgata tcgccacaga tgatgccgca ctggttgagc agatcggtga gagggttcac gtggtggaag gcgaccggtt ggccttcaaa atcaccacga cactcgacat gacattggcc gangcgattc tcggtgctac ggaaggcgtc caataataaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga nttggctgct gccnccnnnn nnnnna

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcggctg ggtgcaaata ccccgaaggc cttcgtgagt gtcggcggcc ggaccatgct tcagcgttgt gtggacggcc tgctcgcatc cggggccgtg gacgaggtag tggtcgtggt cggtgccgat cagctcgaga gggccgctgc cctggtgggc tccggggcca ccgttgtgca gggcggtgcc gagcgcaccg actcggtgcg ggccggtttg gccgccgtgg atgcggccga ctggattctg gttcacgatg ccgcgcgtcc gttgacccct ccggacatga tcgcccgcat cgtcgcagag ctgcgtacgg gccgcggcgc tgtcattccg gcggttgccg tcgcggacac catcaaatcg atcgacgttc acggtgacgt gatcggcact cccgaccggg cgggcctgcg cgccgtacag accccacagg gcttcgccgc cgacgttctc aggcgtgcgt acgcggccgc aggtgatatc gccacagatg atgccgcact ggttgagcag atcggtgaga gggttcacgt ggtggaaggc gaccggttgg ccttcaaaat caccacgaca ctcgacatga cattggccga ggcgattctc ggtgctacgg aaggcgtcca aaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa