MyabA.00155.a

Probable DNA helicase II homolog UvrD2

CENTER ID: MyabA.00155.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00155.a.A1.GE30258 Full length( MyabA.00155.a ) 1 689
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.3580
RefSeq: YP_001704241.1
UniProt: B1MEY1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MARSLLDGLD DEQRAAVTAP RGPVCVLAGA GTGKTRTITH RIAYLVASGH VAAGQILAVT FTQRAAGEMR GRLRDLGVGS AQAVTFHAAG LRQLRYFWPQ LVGDTRWELI DSKFTLVAQA ANRAKVSTTT DTVRDLAGEI EWAKASLISP ETYPAAAAKF GRDAPVPAEQ VAKVYAGYEK LKARPDGSTL LDFDDLLLHT AAAIENSAAV AEEFRDRYRC FVVDEYQDVT PLQQRVLDAW LGPRDDVTVV GDANQTIYSF TGATPQYLLG FSRRFPDATV VRLERDYRST PQVVSLANRL ISAAQGRVAG SKLQLIGQRP QGPAPRFSEH DDEAAEAKSV AKSIAALIES GTPASEVAVL YRINAQSEVY EEALTEAGIP FQVRGGEGFF TRQEVRQALV ALQRAAGRED AGMPVPEQVR EILEPLGLSQ EEPHGTQARE RWESLRALVE LADEECARQP ELDLPGLVRE LRVRAEARHP PTVQGVTLAS LHAAKGLEWD AVFLVGLADG TLPISHALSR AGDIEPVEEE RRLLYVGITR ARVHLNLSWA LSRAAGGRKS RKHSRFLNGI APTAQLPASA TPRRRGGGAR CRVCNAALST PAAVLLRRCE SCPSDIDEEL LVALKDWRLK TAKEMKVPAF VVFTDNTLIA IAELLPGDDA ALVAIPGIGA RKLEQYGPDV LRLVRARSS
NT Sequence
GTGGCGCGCT CTCTTCTCGA TGGTCTCGAT GACGAGCAGC GTGCCGCCGT CACCGCTCCG CGCGGCCCCG TCTGCGTGCT CGCTGGGGCC GGAACAGGCA AGACGCGCAC AATTACCCAT CGCATCGCCT ATTTGGTCGC ATCCGGCCAT GTCGCCGCGG GCCAGATTCT GGCTGTCACC TTCACCCAGC GGGCGGCGGG GGAGATGCGG GGTCGGCTGC GTGACCTGGG TGTCGGATCC GCGCAGGCCG TCACATTTCA CGCGGCCGGT CTACGGCAGC TCCGGTATTT CTGGCCGCAG TTGGTCGGCG ATACCCGCTG GGAGCTGATC GACAGCAAGT TCACCCTCGT CGCGCAGGCC GCGAACCGCG CCAAGGTGAG CACCACCACC GACACCGTGC GTGACCTCGC CGGTGAAATC GAGTGGGCCA AGGCGTCTTT GATCAGCCCG GAGACATATC CGGCCGCCGC CGCCAAGTTC GGCAGGGACG CGCCGGTGCC GGCCGAACAG GTGGCCAAGG TCTACGCGGG CTACGAAAAA CTCAAGGCCC GGCCCGATGG GTCCACCCTT CTCGACTTCG ACGACCTGCT GCTTCATACC GCGGCCGCCA TCGAGAACTC GGCAGCCGTC GCCGAGGAGT TCCGGGACCG TTATCGCTGT TTCGTCGTCG ACGAGTACCA GGACGTGACC CCGCTGCAGC AGCGGGTGCT CGACGCGTGG CTGGGGCCGC GCGATGACGT GACGGTAGTG GGCGACGCCA ACCAGACCAT CTACTCGTTC ACCGGTGCCA CTCCGCAGTA CCTGCTTGGC TTCTCGCGCA GATTTCCCGA TGCGACGGTG GTCCGTCTGG AACGCGACTA TCGTTCGACG CCGCAAGTGG TTTCGCTGGC CAATCGGTTG ATCTCGGCCG CGCAGGGCCG GGTGGCCGGC AGCAAGCTGC AACTGATCGG TCAGCGGCCA CAGGGGCCTG CCCCCAGGTT TTCCGAGCAT GACGACGAGG CGGCAGAGGC CAAGTCTGTT GCGAAATCCA TTGCGGCACT GATTGAATCC GGAACCCCCG CCTCCGAGGT CGCGGTGTTG TACCGCATCA ACGCACAGTC TGAGGTGTAC GAGGAGGCGC TGACCGAGGC CGGCATCCCG TTTCAAGTGC GTGGCGGCGA AGGGTTCTTC ACGCGGCAGG AGGTGCGGCA GGCGTTGGTG GCGCTGCAAC GCGCAGCCGG CCGTGAGGAT GCGGGAATGC CTGTGCCAGA ACAGGTGCGG GAGATTCTGG AACCGTTGGG ATTGTCGCAG GAAGAGCCGC ACGGAACCCA GGCGCGCGAA CGGTGGGAAT CGCTGCGGGC ACTTGTCGAA CTTGCCGACG AGGAGTGTGC TCGTCAGCCG GAGCTGGATC TGCCCGGGCT CGTGCGCGAG CTGCGGGTAC GCGCCGAGGC GCGCCATCCA CCGACCGTGC AGGGCGTGAC GCTGGCGTCG CTGCACGCGG CCAAGGGCCT GGAATGGGAC GCGGTGTTCC TGGTGGGGCT CGCCGACGGC ACCCTACCCA TCTCGCATGC ACTGTCGCGC GCCGGTGATA TCGAGCCGGT GGAGGAAGAG CGCCGCCTGC TGTACGTGGG AATCACTCGC GCCCGTGTGC ATTTGAACTT GAGCTGGGCG CTGTCGCGCG CCGCCGGCGG GCGTAAGTCG CGTAAGCATT CACGATTCCT CAACGGAATC GCCCCGACCG CGCAACTTCC CGCTTCTGCC ACGCCCAGGC GCCGCGGCGG TGGCGCGCGC TGCCGTGTCT GCAATGCGGC GCTGAGCACG CCCGCCGCAG TGCTGCTGCG GCGCTGCGAG AGCTGCCCGT CCGATATCGA CGAGGAACTG CTTGTCGCAC TGAAGGATTG GCGGCTCAAG ACGGCCAAGG AAATGAAGGT TCCGGCGTTC GTGGTCTTCA CCGACAACAC GTTGATCGCC ATCGCCGAAC TGCTTCCCGG CGACGATGCC GCTCTGGTCG CCATTCCCGG GATCGGTGCG CGCAAGCTGG AGCAGTACGG CCCCGACGTA TTGCGTCTGG TGCGTGCCAG ATCCTCG
Details for MyabA.00155.a.A1.GE30258
HARVESTED ON: 8/31/2010
SEQUENCED ON: 9/7/2010
EXPECTED MW: 77kDa
OBSERVED MW: 80kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 18
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVARSLLDG LDDEQRAAVT APRGPVCVLA GAGTGKTRTI THRIAYLVAS GHVAAGQILA VTFTQRAAGE MRGRLRDLGV GSAQAVTFHA AGLRQLRYFW PQLVGDTR
Validated NT Sequence
anattttntt tnnctttaag aaggaganat accatggctc atcaccatca ccatcatatg ggtaccctgg aagctcagac ccagggtcct ggttcgatgg tggcgcgctc tcttctcgat ggtctcgatg acgagcagcg tgccgccgtc accgctccgc gcggccccgt ctgcgtgctc gctggggccg gaacaggcaa gacgcgcaca attacccatc gcatcgccta tttggtcgca tccggccatg tcgccgcggg ccagattctg gctgtcacct tcacccagcg ggcggcgggg gagatgcggg gtcggctgcg tgacctgggt gtcggatccg cgcaggccgt cacatttcac gcggccggtc tacggcagct ccggtatttc tggccgcagt tggtcggcga tacccgctga gcgntnnntc ancaagttca ccctcgtcgc gcaggccgcg aaccgcgcca aggtgagcac caccaccgac accgtgcgtg acctcnncgg tgaaatcgag tgggccnagc gtctttgatc annccggana catatcnngn ngnnnncncn nnnnncngna ngnacgcgnn ngtgccggcc gaacaggtgg ccaaggtcna cncnggctac naaaaactca nngnccggcc cgatgggtcc acccttctcg acttcgacga nctgctgctt cataccgcgg ccgccatcna naactcngca gccgtcnccn annagttcnn gnaccnttat cgctgtttcn tcntcnacna ntaccaggac ntgancccgc tgnnnnntnn nnnnnnnnna ncnng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVARSLLDG LDDEQRAAVT APRGPVCVLA GAGTGKTRTI THRIAYLVAS GHVAAGQILA VTFTQRAAGE MRGRLRDLGV GSAQAVTFHA AGLRQLRYFW PQLVGDTRWE LIDSKFTLVA QAANRAKVST TTDTVRDLAG EIEWAKASLI SPETYPAAAA KFGRDAPVPA EQVAKVYAGY EKLKARPDGS TLLDFDDLLL HTAAAIENSA AVAEEFRDRY RCFVVDEYQD VTPLQQRVLD AWLGPRDDVT VVGDANQTIY SFTGATPQYL LGFSRRFPDA TVVRLERDYR STPQVVSLAN RLISAAQGRV AGSKLQLIGQ RPQGPAPRFS EHDDEAAEAK SVAKSIAALI ESGTPASEVA VLYRINAQSE VYEEALTEAG IPFQVRGGEG FFTRQEVRQA LVALQRAAGR EDAGMPVPEQ VREILEPLGL SQEEPHGTQA RERWESLRAL VELADEECAR QPELDLPGLV RELRVRAEAR HPPTVQGVTL ASLHAAKGLE WDAVFLVGLA DGTLPISHAL SRAGDIEPVE EERRLLYVGI TRARVHLNLS WALSRAAGGR KSRKHSRFLN GIAPTAQLPA SATPRRRGGG ARCRVCNAAL STPAAVLLRR CESCPSDIDE ELLVALKDWR LKTAKEMKVP AFVVFTDNTL IAIAELLPGD DAALVAIPGI GARKLEQYGP DVLRLVRARS S
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggcgcgc tctcttctcg atggtctcga tgacgagcag cgtgccgccg tcaccgctcc gcgcggcccc gtctgcgtgc tcgctggggc cggaacaggc aagacgcgca caattaccca tcgcatcgcc tatttggtcg catccggcca tgtcgccgcg ggccagattc tggctgtcac cttcacccag cgggcggcgg gggagatgcg gggtcggctg cgtgacctgg gtgtcggatc cgcgcaggcc gtcacatttc acgcggccgg tctacggcag ctccggtatt tctggccgca gttggtcggc gatacccgct gggagctgat cgacagcaag ttcaccctcg tcgcgcaggc cgcgaaccgc gccaaggtga gcaccaccac cgacaccgtg cgtgacctcg ccggtgaaat cgagtgggcc aaggcgtctt tgatcagccc ggagacatat ccggccgccg ccgccaagtt cggcagggac gcgccggtgc cggccgaaca ggtggccaag gtctacgcgg gctacgaaaa actcaaggcc cggcccgatg ggtccaccct tctcgacttc gacgacctgc tgcttcatac cgcggccgcc atcgagaact cggcagccgt cgccgaggag ttccgggacc gttatcgctg tttcgtcgtc gacgagtacc aggacgtgac cccgctgcag cagcgggtgc tcgacgcgtg gctggggccg cgcgatgacg tgacggtagt gggcgacgcc aaccagacca tctactcgtt caccggtgcc actccgcagt acctgcttgg cttctcgcgc agatttcccg atgcgacggt ggtccgtctg gaacgcgact atcgttcgac gccgcaagtg gtttcgctgg ccaatcggtt gatctcggcc gcgcagggcc gggtggccgg cagcaagctg caactgatcg gtcagcggcc acaggggcct gcccccaggt tttccgagca tgacgacgag gcggcagagg ccaagtctgt tgcgaaatcc attgcggcac tgattgaatc cggaaccccc gcctccgagg tcgcggtgtt gtaccgcatc aacgcacagt ctgaggtgta cgaggaggcg ctgaccgagg ccggcatccc gtttcaagtg cgtggcggcg aagggttctt cacgcggcag gaggtgcggc aggcgttggt ggcgctgcaa cgcgcagccg gccgtgagga tgcgggaatg cctgtgccag aacaggtgcg ggagattctg gaaccgttgg gattgtcgca ggaagagccg cacggaaccc aggcgcgcga acggtgggaa tcgctgcggg cacttgtcga acttgccgac gaggagtgtg ctcgtcagcc ggagctggat ctgcccgggc tcgtgcgcga gctgcgggta cgcgccgagg cgcgccatcc accgaccgtg cagggcgtga cgctggcgtc gctgcacgcg gccaagggcc tggaatggga cgcggtgttc ctggtggggc tcgccgacgg caccctaccc atctcgcatg cactgtcgcg cgccggtgat atcgagccgg tggaggaaga gcgccgcctg ctgtacgtgg gaatcactcg cgcccgtgtg catttgaact tgagctgggc gctgtcgcgc gccgccggcg ggcgtaagtc gcgtaagcat tcacgattcc tcaacggaat cgccccgacc gcgcaacttc ccgcttctgc cacgcccagg cgccgcggcg gtggcgcgcg ctgccgtgtc tgcaatgcgg cgctgagcac gcccgccgca gtgctgctgc ggcgctgcga gagctgcccg tccgatatcg acgaggaact gcttgtcgca ctgaaggatt ggcggctcaa gacggccaag gaaatgaagg ttccggcgtt cgtggtcttc accgacaaca cgttgatcgc catcgccgaa ctgcttcccg gcgacgatgc cgctctggtc gccattcccg ggatcggtgc gcgcaagctg gagcagtacg gccccgacgt attgcgtctg gtgcgtgcca gatcctcgaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa