MyabA.00148.a

Tyrosine recombinase XerD

CENTER ID: MyabA.00148.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00148.a.A1.GE30255 Full length( MyabA.00148.a ) 1 315
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.2409
RefSeq: YP_001703101.1
UniProt: B1MB26

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTAAVGLLDG EIQSYLDHLD VERGVAANTL SSYRRDLRRY QQHLADRKID RLAEVCETDV SDFVVTLRRG DPANGVPELS ASSAARALIA VRGLHRFAAI EGLAPTDVAR AVKPPTPNRR LPKSLTVEQV EALLNAAGGG GSDGPLDLRN RALLELLYST GARISEAVGL DVDDVDVQAR SVLLWGKGGK QRLVPVGRPA VEALQAYLVR GRPDLARRGR GGVPALFLNS RGGRLSRQSA WQVLADAAER AKISAAVSPH TLRHSFATHL LEGGADVRVV QELLGHASVT TTQIYTLVTV SALREVWAGA HPRAR
NT Sequence
GTGACGGCCG CGGTCGGGCT CCTTGACGGC GAGATCCAGT CCTACCTGGA TCATCTCGAC GTGGAACGCG GCGTCGCCGC CAATACGCTG AGCTCCTACC GTCGGGACCT GCGCCGCTAC CAGCAGCATC TCGCCGACCG CAAGATCGAC CGGTTGGCCG AGGTCTGCGA AACCGATGTC AGCGATTTCG TGGTCACGCT GCGCCGGGGC GATCCGGCGA ACGGAGTGCC CGAGCTGTCC GCGTCCTCGG CGGCCCGCGC ATTGATCGCC GTGCGAGGGC TGCACCGGTT TGCCGCCATC GAGGGGCTGG CGCCCACGGA TGTCGCCCGT GCGGTGAAAC CGCCGACCCC CAACCGCCGG CTGCCGAAGA GCCTGACGGT CGAACAGGTG GAAGCACTGC TGAATGCCGC GGGTGGCGGC GGCTCGGACG GACCACTGGA TCTGCGCAAC CGTGCACTGC TGGAGCTGCT GTACTCGACC GGAGCACGCA TCTCCGAGGC CGTGGGGCTG GACGTCGACG ATGTGGATGT GCAGGCTCGT TCGGTGCTGC TGTGGGGCAA GGGCGGCAAG CAGCGCCTGG TGCCGGTGGG TCGGCCTGCC GTAGAGGCGC TGCAGGCATA CCTGGTGCGT GGCCGTCCCG ATCTGGCACG CCGGGGACGC GGCGGCGTCC CCGCCCTGTT TCTGAACTCC CGGGGCGGAC GGCTTTCCCG CCAGAGCGCA TGGCAGGTGC TGGCCGACGC CGCCGAACGG GCCAAGATCA GCGCGGCGGT TTCCCCGCAC ACCCTGCGAC ATTCCTTTGC GACACATCTG CTGGAGGGCG GCGCCGACGT TCGTGTTGTC CAGGAGTTAC TCGGCCATGC GTCGGTGACA ACGACCCAGA TCTACACGTT GGTCACGGTC AGTGCGCTGC GCGAAGTATG GGCAGGTGCG CATCCCCGTG CCCGT
Details for MyabA.00148.a.A1.GE30255
HARVESTED ON: 8/31/2010
SEQUENCED ON: 9/7/2010
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMVTAAVG LLDGEIQSYL DHLDVERGVA ANTLSSYRRD LRRYQQHLAD RKIDRLAEVC ETDVSDFVVT LRRGDPANGV PELSASSAAR ALIAVRGLHR FAAIEGLAPT DVARAVKPPT PNRRLPKSLT VEQVEALLNA AGGGGSDGPL DLRNRALLEL LYSTGARISE AVGLDVDDVD VQARSVLLWG KGGKQRLVPV GRPAVEALQA YLVRGRPDLA RRGRGGVPAL FLNSRGGRLS RQSAWQVLAD AAERAKISAA VSPHTLRHSF ATHLLEGGAD VRVVQELLGH ASVTTTQIYT LVTVSALREV WAGAHPRAR
Validated NT Sequence
ttattaacgg gcacggggat gcgcacctgc ccatacttcg cgcagcgcac tgaccgtgac caacgtgtag atctgggtcg ttgtcaccga cgcatggccg agtaactcct ggacaacacg aacgtcggcg ccgccctcca gcagatgtgt cgcaaaggaa tgtcgcaggg tgtgcgggga aaccgccgcg ctgatcttgg cccgttcggc ggcgtcggcc agcacctgcc atgcgctctg gcgggaaagc cgtccgcccc gggagttcag aaacagggcg gggacgccgc cgcgtccccg gcgtgccaga tcgggacggc cacgcaccag gtatgcctgc agcgcctcta cggcaggccg acccaccggc accaggcgct gcttgccgcc cttgccccac agcagcaccg aacgagcctg cacatccaca tcgtcgacgt ccagccccac ggcctcggag atgcgtgctc cggtcgagta cagcagctcc agcagtgcac ggttgcgcag atccagtggt ccgtccgagc cgccgccacc cgcggcattc agcagtgctt ccacctgttc gaccgtcagg ctcttcggca gccggcggtt gggggtcggc ggtttcaccg cacgggcgac atccgtgggc gccagcccct cgatggcggc aaaccggtgc agccctcgca cggcgatcaa tgcgcgggcc gccgaggacg cggacagctc gggcactccg ttcgccggat cgccccggcg cagcgtgacc acgaaatcgc tgacatcggt ttcgcagacc tcggccaacc ggtcgatctt gcggtcggcg agatgctgct ggtagcggcg caggtcccga cggtaggagc tcagcgtatt ggcggcgacg ccgcgttcca cgtcgagatg atccaggtag gactggatct cgccgtcaag gagcccgacc gcggccgtca ccatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTAAVGLL DGEIQSYLDH LDVERGVAAN TLSSYRRDLR RYQQHLADRK IDRLAEVCET DVSDFVVTLR RGDPANGVPE LSASSAARAL IAVRGLHRFA AIEGLAPTDV ARAVKPPTPN RRLPKSLTVE QVEALLNAAG GGGSDGPLDL RNRALLELLY STGARISEAV GLDVDDVDVQ ARSVLLWGKG GKQRLVPVGR PAVEALQAYL VRGRPDLARR GRGGVPALFL NSRGGRLSRQ SAWQVLADAA ERAKISAAVS PHTLRHSFAT HLLEGGADVR VVQELLGHAS VTTTQIYTLV TVSALREVWA GAHPRAR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgacggcc gcggtcgggc tccttgacgg cgagatccag tcctacctgg atcatctcga cgtggaacgc ggcgtcgccg ccaatacgct gagctcctac cgtcgggacc tgcgccgcta ccagcagcat ctcgccgacc gcaagatcga ccggttggcc gaggtctgcg aaaccgatgt cagcgatttc gtggtcacgc tgcgccgggg cgatccggcg aacggagtgc ccgagctgtc cgcgtcctcg gcggcccgcg cattgatcgc cgtgcgaggg ctgcaccggt ttgccgccat cgaggggctg gcgcccacgg atgtcgcccg tgcggtgaaa ccgccgaccc ccaaccgccg gctgccgaag agcctgacgg tcgaacaggt ggaagcactg ctgaatgccg cgggtggcgg cggctcggac ggaccactgg atctgcgcaa ccgtgcactg ctggagctgc tgtactcgac cggagcacgc atctccgagg ccgtggggct ggacgtcgac gatgtggatg tgcaggctcg ttcggtgctg ctgtggggca agggcggcaa gcagcgcctg gtgccggtgg gtcggcctgc cgtagaggcg ctgcaggcat acctggtgcg tggccgtccc gatctggcac gccggggacg cggcggcgtc cccgccctgt ttctgaactc ccggggcgga cggctttccc gccagagcgc atggcaggtg ctggccgacg ccgccgaacg ggccaagatc agcgcggcgg tttccccgca caccctgcga cattcctttg cgacacatct gctggagggc ggcgccgacg ttcgtgttgt ccaggagtta ctcggccatg cgtcggtgac aacgacccag atctacacgt tggtcacggt cagtgcgctg cgcgaagtat gggcaggtgc gcatccccgt gcccgtaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa