SSGCID: Target Page

MyabA.00139.a

Dephospho-CoA kinase CoaE

CENTER ID:	MyabA.00139.a
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.00139.a.B2.GE40714	nterm domain		1	204

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|36809.5.peg.2342
UniProt:	B1MAV8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLRIGLTGGI GAGKSTVSRT LAGCGAFIVD SDVIAREVVE PGTPGLAALV EAFGETILRA DGALDRPALA AIAFQDDEHR ATLNGIVHPL VGARRAELIA AAGEEAVVVE DIPLLTENSL APFYHLVLVV HADIETRVTR LVTHRGMNEQ DARSRVAAQA SDEQRRAIAD VWLDNSGDTE VLAHAVRDLW ASRLLPYAHN LRMRQRAPWR PELMPADPTW PVQAQRVINR LAVACGSRAL RIDHIGSTAV PGLDAKDVID IQVTVESLAV ADTLADPLAG IGLPSIPGIT TDDPHDSDTA HWGKRIHAAA DPGRAVLVHV RVDGWPGQRF ALIFRDWLRS DHQACAEYLS IKQAAERAAA AAGGDPGAYV DAKEPWFAQA YTRALDWANA SQWTP

NT Sequence

atgttgcgta tcggcctaac cggtggaatc ggtgcgggga agtccaccgt ctcgcggacc ctcgccgggt gcggggcttt catcgtggac agcgatgtga tcgcacgcga ggtggtggaa cccggaacgc ctgggctggc ggcgctcgtg gaggcctttg gagaaacgat actgcgtgcc gacggtgccc tggatcgtcc ggcattggcg gccatcgcat tccaggacga tgagcaccgc gccactctca atgggatagt gcatccgttg gtgggggcgc gccgggctga actgattgcg gctgcgggtg aggaagctgt tgtggtggaa gatattccgc tgctcaccga aaactcactc gcgccgtttt atcacctggt tctggtcgtt cacgcggaca tcgaaactcg ggtgacacgg cttgtcactc atcgtgggat gaatgagcag gacgcccgct cgcgcgtcgc cgcgcaggcc tccgatgagc agcgccgcgc gatcgcggat gtgtggctgg acaactctgg cgataccgag gtgcttgcgc acgcggtgcg tgatctgtgg gcttcccggc tgctgcccta cgcacataat ctgcgcatgc ggcaacgcgc gccgtggcgg cccgagctga tgcctgcgga tccgacgtgg ccggtgcagg cgcagcgcgt catcaatcgc cttgcggtgg cttgtggctc gcgggcgctg cggatcgacc atatcggctc cacggcagta cctggtctgg atgccaaaga cgtcatcgat atccaggtga cggtggagtc acttgccgtg gccgacacgc tggcggatcc cttagcgggc atcgggttgc cgtcgatccc gggtatcacc acagatgacc cacatgactc tgacaccgcc cattggggta agcgcatcca tgccgcagcc gatccgggac gcgccgttct ggtgcacgtg agagtggacg gctggccggg gcagcgattc gcgctgattt tccgggattg gctgcggtcc gaccaccagg cgtgcgccga gtatctttcg atcaagcagg cggctgagcg ggcggcggct gcggccggcg gcgatcccgg cgcctacgtg gatgccaagg agccttggtt cgcccaggcg tacacgcggg cactggattg ggccaatgca tcccaatgga cgccttag

Details for MyabA.00139.a.B2.GE40714

HARVESTED ON:	11/8/2016
SEQUENCED ON:	11/8/2016
EXPECTED MW:	23kDa
OBSERVED MW:	23kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML RIGLTGGIGA GKSTVSRTLA GCGAFIVDSD VIAREVVEPG TPGLAALVEA FGETILRADG ALDRPALAAI AFQDDEHRAT LNGIVHPLVG ARRAELIAAA GEEAVVVEDI PLLTENSLAP FYHLVLVVHA DIETRVTRLV THRGMNEQDA RSRVAAQASD EQRRAIADVW LDNSGDTEVL AHAVRDLWAS RLLPYAHNLR MR

Validated NT Sequence

atggctcacc accaccacca ccatatgttg cgtatcggcc taaccggtgg aatcggtgcg gggaagtcca ccgtctcgcg gaccctcgcc gggtgcgggg ctttcatcgt ggacagcgat gtgatcgcac gcgaggtggt ggaacccgga acgcctgggc tggcggcgct cgtggaggcc tttggagaaa cgatactgcg tgccgacggt gccctggatc gtccggcatt ggcggccatc gcattccagg acgatgagca ccgcgccact ctcaatggga tagtgcatcc gttggtgggg gcgcgccggg ctgaactgat tgcggctgcg ggtgaggaag ctgttgtggt ggaagatatt ccgctgctca ccgaaaactc actcgcgccg ttttatcacc tggttctggt cgttcacgcg gacatcgaaa ctcgggtgac acggcttgtc actcatcgtg ggatgaatga gcaggacgcc cgctcgcgcg tcgccgcgca ggcctccgat gagcagcgcc gcgcgatcgc ggatgtgtgg ctggacaact ctggcgatac cgaggtgctt gcgcacgcgg tgcgtgatct gtgggcttcc cggctgctgc cctacgcaca taatctgcgc atgcggtaag tgagtaagat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttgc gtatcggcct aaccggtgga atcggtgcgg ggaagtccac cgtctcgcgg accctcgccg ggtgcggggc tttcatcgtg gacagcgatg tgatcgcacg cgaggtggtg gaacccggaa cgcctgggct ggcggcgctc gtggaggcct ttggagaaac gatactgcgt gccgacggtg ccctggatcg tccggcattg gcggccatcg cattccagga cgatgagcac cgcgccactc tcaatgggat agtgcatccg ttggtggggg cgcgccgggc tgaactgatt gcggctgcgg gtgaggaagc tgttgtggtg gaagatattc cgctgctcac cgaaaactca ctcgcgccgt tttatcacct ggttctggtc gttcacgcgg acatcgaaac tcgggtgaca cggcttgtca ctcatcgtgg gatgaatgag caggacgccc gctcgcgcgt cgccgcgcag gcctccgatg agcagcgccg cgcgatcgcg gatgtgtggc tggacaactc tggcgatacc gaggtgcttg cgcacgcggt gcgtgatctg tgggcttccc ggctgctgcc ctacgcacat aatctgcgca tgcggtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat