MyabA.00113.a

Putative polyketide synthase MbtC

CENTER ID: MyabA.00113.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00113.a.A1.GE30253 Full length( MyabA.00113.a ) 1 441
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.2159
RefSeq: YP_001702855.1
UniProt: B1MPF0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTAVHVDPVV ISGMAIEAPG GIDTPAAFWN ALADGRELIS AFPRDRGWPV DELLSLSNLD GWGRISDAGG FITDASAFDP TFYGITQREA VAMDPQQRVA MRVAWKALEN TGLNPGAVDG SEAGCFIGIS PNEYGPPVGE VNAHSGMRVV GRGQLGAAGR ISHGLGLIGP SVCVDSACAS SLAALHLAAS AVRADECEWA IAGGVCVMGS PGGFYEFSRM HALDPDGHCR SYSDEANGTL WGEAVGMVVL ERESRARALG HRVYGRIMAV RTNHNGGGKP ILVPRGRAQA KLVAATIAAS GVDPADVGML EGHGTGTRAG DPLEILALQS TYGAGGSNAL LGSAKSNAGH AQSAAGMVGL IKLLLSGQHG HIPPTLFADN PTTKIDWSMT GIRLATKLHP WEPKDGIRYG SVSSFGAGGA NAHAIIAMPA MDAQGEDGDD F
NT Sequence
ATGACCGCCG TTCACGTTGA CCCCGTCGTC ATCTCCGGAA TGGCGATCGA GGCGCCGGGT GGGATCGACA CCCCGGCGGC ATTCTGGAAT GCGCTGGCCG ACGGTCGCGA ACTCATCTCG GCGTTCCCCC GTGACCGTGG CTGGCCAGTG GACGAGCTGC TATCGCTGTC GAACCTGGAC GGCTGGGGCC GCATTTCCGA TGCCGGCGGA TTCATCACCG ACGCAAGCGC TTTCGACCCC ACTTTCTACG GCATCACGCA GCGTGAGGCC GTGGCGATGG ATCCGCAGCA GCGGGTAGCC ATGCGGGTCG CGTGGAAGGC GCTGGAAAAC ACCGGGCTCA ATCCGGGAGC AGTCGACGGA TCGGAAGCCG GTTGTTTCAT CGGCATCTCC CCTAACGAAT ACGGGCCGCC AGTCGGTGAA GTCAACGCGC ACAGCGGAAT GCGTGTCGTC GGCCGCGGAC AGCTGGGTGC CGCGGGCCGA ATCTCGCACG GCCTGGGCCT CATCGGCCCA TCCGTCTGTG TCGACTCCGC GTGTGCTTCG TCGTTGGCGG CGCTGCATCT CGCGGCGTCC GCCGTTCGTG CGGACGAATG CGAGTGGGCC ATCGCCGGCG GAGTCTGTGT CATGGGCTCG CCCGGAGGGT TTTACGAATT TTCTCGCATG CACGCGCTCG ACCCCGACGG GCACTGCCGC TCCTATTCGG ACGAGGCCAA CGGCACACTC TGGGGCGAGG CCGTGGGAAT GGTCGTACTG GAGCGCGAGT CACGGGCGCG CGCCCTGGGA CACCGGGTGT ACGGACGCAT CATGGCCGTA CGCACCAACC ACAACGGCGG CGGCAAACCC ATTCTCGTGC CACGGGGACG GGCACAGGCC AAGCTGGTCG CCGCCACCAT CGCCGCTTCC GGTGTGGATC CGGCCGATGT CGGGATGCTC GAGGGGCACG GCACGGGAAC GCGTGCCGGC GACCCGCTGG AGATCCTGGC GCTGCAAAGC ACATACGGCG CGGGCGGCTC GAATGCCCTG CTAGGGTCCG CGAAATCCAA TGCCGGACAC GCACAATCCG CCGCCGGCAT GGTGGGTCTG ATAAAGCTCC TGCTGTCCGG ACAACACGGG CATATCCCGC CGACGCTCTT CGCGGACAAC CCCACCACCA AGATTGACTG GTCGATGACC GGGATTCGCC TGGCCACGAA GCTGCACCCG TGGGAACCCA AAGACGGCAT CCGCTACGGC TCGGTCTCGT CCTTCGGAGC GGGGGGCGCC AACGCTCATG CCATCATCGC GATGCCCGCC ATGGACGCAC AGGGAGAGGA CGGGGACGAT TTC
Details for MyabA.00113.a.A1.GE30253
HARVESTED ON: 8/31/2010
SEQUENCED ON: 9/7/2010
EXPECTED MW: 47kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 78
PERCENT COVERAGE: 83
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTAVHVDPV VISGMAIEAP GGIDTPAAFW NALADGRELI SAFPRDRGWP VDELLSLSNL DGWGRISDAG GFITDASAFD PTFYGITQRE AVAMDPQQRV AMRVAWKALE NTGLNPGAVD GSEAGCFIGI SPNEYGPPVG EVNAHSGMRV VGRGQLGAAG RISHGLGLIG PSVCVDSACA SSLAALHLAA SAVRADECEW AIAGGVCVMG SPGGFYEFSR MHALDPDGHC RSYSDXANGT LWGXAVGMVV LXRESRARAL GXRXXXRIMX VRTNHXGXGX PILVPRGXXQ XXLVAAXIXX FXXXXXXXXX SXXXXGXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXGXX XXXXXXXXXX XXXXXX
Validated NT Sequence
attttnttta ntttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaccg ccgttcacgt tgaccccgtc gtcatctccg gaatggcgat cgaggcgccg ggtgggatcg acaccccggc ggcattctgg aatgcgctgg ccgacggtcg cgaactcatc tcggcgttcc cccgtgaccg tggctggcca gtggacgagc tgctatcgct gtcgaacctg gacggctggg gccgcatttc cgatgccggc ggattcatca ccgacgcaag cgctttcgac cccactttct acggcatcac gcagcgtgag gccgtggcga tggatccgca gcagcgggta gccatgcggg tcgcgtggaa ggcgctggaa aacaccgggc tcaatccggg agcagtcgac ggatcggaag ccggttgttt catcggcatc tcccctaacg aatacgggcc gccagtcggt gaagtcaacg cgcacagcgg aatgcgtgtc gtcggccgcg gacagctggg tgccgcgggc cgaatctcgc acggcctggg cctcatcggc ccatccgtct gtgtcgactc cgcgtgtgct tcgtcgttgg cggcgctgca tctcgcggcg tccgccgttc gtgcggacga atgcgagtgg gccatcgccg gcggagtctg tgtcatgggc tcgcccggag ggttttacga attttctcgc atgcacgcgc tcgaccccga cgggcactgc cgctcctatt cggacgangc caacggcaca ctctggggcg angccgtggg aatggtcgta ctggnncgcg agtcacgggc gcgcgccctg ggnnaccggg ngtanngacg catcatggnc gtacgcacca accacnacgg ngnnggnnaa cccattctcg tgccncgggg nngggnacag gncnagctgg tcgccgccnn catcgncnnc ttcngngngg nncngncgan gnnnnnatgn tcgnnngnnn ggnnngggan cncnnnnnnc nancnnntnn nnancnnnnc tnnaannnnn tangnncngg nngntnnnan nncnnnntnn nnnannnnnn nnnnnnnngn ngggnnnnan nnaannnnnn nntnnnnnnn nnnnnnnnnn nggnnnnnnn nnnnnnnant
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTAVHVDPV VISGMAIEAP GGIDTPAAFW NALADGRELI SAFPRDRGWP VDELLSLSNL DGWGRISDAG GFITDASAFD PTFYGITQRE AVAMDPQQRV AMRVAWKALE NTGLNPGAVD GSEAGCFIGI SPNEYGPPVG EVNAHSGMRV VGRGQLGAAG RISHGLGLIG PSVCVDSACA SSLAALHLAA SAVRADECEW AIAGGVCVMG SPGGFYEFSR MHALDPDGHC RSYSDEANGT LWGEAVGMVV LERESRARAL GHRVYGRIMA VRTNHNGGGK PILVPRGRAQ AKLVAATIAA SGVDPADVGM LEGHGTGTRA GDPLEILALQ STYGAGGSNA LLGSAKSNAG HAQSAAGMVG LIKLLLSGQH GHIPPTLFAD NPTTKIDWSM TGIRLATKLH PWEPKDGIRY GSVSSFGAGG ANAHAIIAMP AMDAQGEDGD DF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgccgtt cacgttgacc ccgtcgtcat ctccggaatg gcgatcgagg cgccgggtgg gatcgacacc ccggcggcat tctggaatgc gctggccgac ggtcgcgaac tcatctcggc gttcccccgt gaccgtggct ggccagtgga cgagctgcta tcgctgtcga acctggacgg ctggggccgc atttccgatg ccggcggatt catcaccgac gcaagcgctt tcgaccccac tttctacggc atcacgcagc gtgaggccgt ggcgatggat ccgcagcagc gggtagccat gcgggtcgcg tggaaggcgc tggaaaacac cgggctcaat ccgggagcag tcgacggatc ggaagccggt tgtttcatcg gcatctcccc taacgaatac gggccgccag tcggtgaagt caacgcgcac agcggaatgc gtgtcgtcgg ccgcggacag ctgggtgccg cgggccgaat ctcgcacggc ctgggcctca tcggcccatc cgtctgtgtc gactccgcgt gtgcttcgtc gttggcggcg ctgcatctcg cggcgtccgc cgttcgtgcg gacgaatgcg agtgggccat cgccggcgga gtctgtgtca tgggctcgcc cggagggttt tacgaatttt ctcgcatgca cgcgctcgac cccgacgggc actgccgctc ctattcggac gaggccaacg gcacactctg gggcgaggcc gtgggaatgg tcgtactgga gcgcgagtca cgggcgcgcg ccctgggaca ccgggtgtac ggacgcatca tggccgtacg caccaaccac aacggcggcg gcaaacccat tctcgtgcca cggggacggg cacaggccaa gctggtcgcc gccaccatcg ccgcttccgg tgtggatccg gccgatgtcg ggatgctcga ggggcacggc acgggaacgc gtgccggcga cccgctggag atcctggcgc tgcaaagcac atacggcgcg ggcggctcga atgccctgct agggtccgcg aaatccaatg ccggacacgc acaatccgcc gccggcatgg tgggtctgat aaagctcctg ctgtccggac aacacgggca tatcccgccg acgctcttcg cggacaaccc caccaccaag attgactggt cgatgaccgg gattcgcctg gccacgaagc tgcacccgtg ggaacccaaa gacggcatcc gctacggctc ggtctcgtcc ttcggagcgg ggggcgccaa cgctcatgcc atcatcgcga tgcccgccat ggacgcacag ggagaggacg gggacgattt caaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa