SSGCID: Target Page

MeloA.20507.a

Formate acetyltransferase (PFL)

CENTER ID:	MeloA.20507.a
ORGANISM:	Megasphaera lornae UPII 199-6
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MeloA.20507.a.A1.GE46213	full length		1	751

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MeloA.20507.a.A1.PW39574	full length		1	751

External Resources

RESOURCE	REFERENCE ID
UniProt:	D3LWI4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVLENKGWEG FTGRLWREEC NVRDFIQNNY QPYEGDERFL QAPTEATDIL WGKVQEYQKA EREHGGVLKE ETEIVSSITA YGPGYIDPQM KELEKVVGLQ TDEPLKRAFM PYGGIKMAEE ALEMYNYKLN PELHKVFTEY HKTHNEAVFD AYTKEMLQAR HTHIITGLPD AYARGRIVGD YRRVALYGID QLIAWKERDK EYNDDGIMTD NVIRLREEIA EQIKALKQMK ELANIYGYDI SKPATNAREA VQWLYFGYLA AIKTQNGAAM SVGRISTFLD IYIERDLAAG EITEQEAQEL IDHLTLKFRI VKFARIRSYN ELFSGDPIWA TLEMAGIGMD GRSMVTKTDF RFLHTLENMG PAPEPNLTVL YSSQLPHPFK KYAAHISDTT SSIQYENDDV MKPVWGDDYS ICCCVSATQT GKEMQFFGAR ANLAKALLYA INGGVDEKFG IQAGPELAPI TSEYLDYDEV VHKYKLMLDW LAGLYVNVLN TIHYMHDKYY YEAAEMALLD TKLRRTFATG IAGFSHVIDS LSAIKYAKVK VIRNDKGLAE DFQVEGDFPK YGNDDDRADE IGVAVLREFM DDIRKRHTYR DSEATTSILT ITSNVVYGKF TGNLPDGRRA WKPFAPGANP SYGAEEKGLM ASLNSVAKLP YTWALDGISN TQSIIPEALG HNEEERAENL VHVLDGYFDQ GAHHLNVNIF GKEKLLDAME HPEKPEYANF TIRVSGYAVK FISLTKEQQL DVIARTFHEK R

NT Sequence

atggtgttgg aaaacaaagg atgggaaggt tttacaggaa gactttggcg agaagagtgt aatgttcggg attttattca aaataattat cagccgtatg aaggtgacga acggttttta caagcgccta cagaggccac cgatatattg tggggcaagg ttcaggaata tcaaaaagcg gaacgggaac acggcggtgt tttaaaagaa gaaacggaaa tcgtttcctc tattacggcg tatggccccg gctatattga tccgcaaatg aaagagttgg aaaaagtagt cggcttgcag acggatgaac cgttgaaacg tgcattcatg ccgtacggcg gcattaaaat ggcggaagaa gccttggaaa tgtacaatta caaattgaat ccggaactgc ataaggtgtt tacggaatat cataaaacac acaatgaagc ggtttttgat gcgtatacta aggaaatgtt gcaggctcgg cacacccata tcattacggg gcttcccgat gcgtatgcac gcggcagaat tgtcggcgat tatcgtcggg tggcgttata cgggattgat cagctcattg cctggaagga acgggataaa gagtataatg atgacgggat tatgacggat aatgtcattc gtttgcggga agaaatcgcc gaacagatta aagcgttgaa gcaaatgaaa gaattggcca acatttacgg ctacgatatt tccaaaccgg caaccaatgc gcgggaagcc gtacagtggt tatatttcgg gtatttggcg gcaattaaga cgcaaaacgg cgcggctatg tcggtaggtc gtatttcgac gttcctggat atttatattg aacgcgactt ggcggccggc gagattacgg aacaggaagc acaagaattg attgaccact tgacgctgaa attccggatt gtcaaattcg cccgcatccg ctcgtacaat gaattgttct cgggcgatcc catttgggct accttggaaa tggccggcat cggcatggac ggtcgttcca tggtaacgaa gacggacttc cgcttcctcc atacgctgga aaatatggga ccggctccgg aaccgaacct gacggtactg tattcgtcgc agttgccgca tccgtttaag aaatatgcgg ctcatatttc ggatacgacg agctccattc agtatgaaaa cgatgatgtc atgaaacccg tttggggcga tgactattct atttgctgct gcgtatcggc aacccagaca gggaaagaaa tgcagttctt cggcgcccgc gccaacttgg caaaagcgtt gttatatgcg attaacggcg gcgtggatga aaaattcgga atacaagcag gtccggaatt ggcgccgatt acttcggaat acttggatta tgatgaagtc gtacataaat ataaactcat gctggattgg ttggcaggat tgtatgtcaa cgttttgaat accatccatt atatgcatga taaatattat tatgaagctg cggaaatggc cttgttggat acgaaacttc gtcgtacctt tgccaccggg attgccgggt tctcgcatgt tatcgactcg ttgtcggcga ttaagtatgc gaaagttaaa gttatccgca acgataaggg cttagcggaa gacttccaag ttgaaggcga ttttccgaaa tacgggaatg atgatgatcg tgccgatgaa atcggtgtag ctgttttgcg ggaatttatg gatgatattc gtaaacggca tacgtatcgc gattcggaag caaccacttc gattcttacg attacgtcga atgtggtata cggcaaattt acaggaaatt tgccggacgg tcgtcgggcg tggaaaccct tcgctcccgg tgcgaatccc agttacggag ccgaagaaaa agggttaatg gcctcgttga actcggtggc aaaattgccg tatacttggg cgttggacgg tatttccaat acgcagtcca ttattccgga agcgttggga cataatgaag aagaacgggc ggaaaatttg gtacatgtat tggacggtta cttcgatcag ggcgctcatc acttaaatgt taatattttc ggaaaagaaa aattattgga tgccatggaa catccggaaa aaccggaata tgcaaatttt acgattcgtg tatccggcta tgccgttaaa tttatttcgt tgaccaaaga acaacagttg gatgttattg cacggacgtt ccatgaaaaa aggtaa

Details for MeloA.20507.a.A1.GE46213

HARVESTED ON:	2/10/2026
SEQUENCED ON:	data unavailable
EXPECTED MW:	88kDa
OBSERVED MW:	88kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVLENKGWE GFTGRLWREE CNVRDFIQNN YQPYEGDERF LQAPTEATDI LWGKVQEYQK AEREHGGVLK EETEIVSSIT AYGPGYIDPQ MKELEKVVGL QTDEPLKRAF MPYGGIKMAE EALEMYNYKL NPELHKVFTE YHKTHNEAVF DAYTKEMLQA RHTHIITGLP DAYARGRIVG DYRRVALYGI DQLIAWKERD KEYNDDGIMT DNVIRLREEI AEQIKALKQM KELANIYGYD ISKPATNARE AVQWLYFGYL AAIKTQNGAA MSVGRISTFL DIYIERDLAA GEITEQEAQE LIDHLTLKFR IVKFARIRSY NELFSGDPIW ATLEMAGIGM DGRSMVTKTD FRFLHTLENM GPAPEPNLTV LYSSQLPHPF KKYAAHISDT TSSIQYENDD VMKPVWGDDY SICCCVSATQ TGKEMQFFGA RANLAKALLY AINGGVDEKF GIQAGPELAP ITSEYLDYDE VVHKYKLMLD WLAGLYVNVL NTIHYMHDKY YYEAAEMALL DTKLRRTFAT GIAGFSHVID SLSAIKYAKV KVIRNDKGLA EDFQVEGDFP KYGNDDDRAD EIGVAVLREF MDDIRKRHTY RDSEATTSIL TITSNVVYGK FTGNLPDGRR AWKPFAPGAN PSYGAEEKGL MASLNSVAKL PYTWALDGIS NTQSIIPEAL GHNEEERAEN LVHVLDGYFD QGAHHLNVNI FGKEKLLDAM EHPEKPEYAN FTIRVSGYAV KFISLTKEQQ LDVIARTFHE KR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgttggaa aacaaaggat gggaaggttt tacaggaaga ctttggcgag aagagtgtaa tgttcgggat tttattcaaa ataattatca gccgtatgaa ggtgacgaac ggtttttaca agcgcctaca gaggccaccg atatattgtg gggcaaggtt caggaatatc aaaaagcgga acgggaacac ggcggtgttt taaaagaaga aacggaaatc gtttcctcta ttacggcgta tggccccggc tatattgatc cgcaaatgaa agagttggaa aaagtagtcg gcttgcagac ggatgaaccg ttgaaacgtg cattcatgcc gtacggcggc attaaaatgg cggaagaagc cttggaaatg tacaattaca aattgaatcc ggaactgcat aaggtgttta cggaatatca taaaacacac aatgaagcgg tttttgatgc gtatactaag gaaatgttgc aggctcggca cacccatatc attacggggc ttcccgatgc gtatgcacgc ggcagaattg tcggcgatta tcgtcgggtg gcgttatacg ggattgatca gctcattgcc tggaaggaac gggataaaga gtataatgat gacgggatta tgacggataa tgtcattcgt ttgcgggaag aaatcgccga acagattaaa gcgttgaagc aaatgaaaga attggccaac atttacggct acgatatttc caaaccggca accaatgcgc gggaagccgt acagtggtta tatttcgggt atttggcggc aattaagacg caaaacggcg cggctatgtc ggtaggtcgt atttcgacgt tcctggatat ttatattgaa cgcgacttgg cggccggcga gattacggaa caggaagcac aagaattgat tgaccacttg acgctgaaat tccggattgt caaattcgcc cgcatccgct cgtacaatga attgttctcg ggcgatccca tttgggctac cttggaaatg gccggcatcg gcatggacgg tcgttccatg gtaacgaaga cggacttccg cttcctccat acgctggaaa atatgggacc ggctccggaa ccgaacctga cggtactgta ttcgtcgcag ttgccgcatc cgtttaagaa atatgcggct catatttcgg atacgacgag ctccattcag tatgaaaacg atgatgtcat gaaacccgtt tggggcgatg actattctat ttgctgctgc gtatcggcaa cccagacagg gaaagaaatg cagttcttcg gcgcccgcgc caacttggca aaagcgttgt tatatgcgat taacggcggc gtggatgaaa aattcggaat acaagcaggt ccggaattgg cgccgattac ttcggaatac ttggattatg atgaagtcgt acataaatat aaactcatgc tggattggtt ggcaggattg tatgtcaacg ttttgaatac catccattat atgcatgata aatattatta tgaagctgcg gaaatggcct tgttggatac gaaacttcgt cgtacctttg ccaccgggat tgccgggttc tcgcatgtta tcgactcgtt gtcggcgatt aagtatgcga aagttaaagt tatccgcaac gataagggct tagcggaaga cttccaagtt gaaggcgatt ttccgaaata cgggaatgat gatgatcgtg ccgatgaaat cggtgtagct gttttgcggg aatttatgga tgatattcgt aaacggcata cgtatcgcga ttcggaagca accacttcga ttcttacgat tacgtcgaat gtggtatacg gcaaatttac aggaaatttg ccggacggtc gtcgggcgtg gaaacccttc gctcccggtg cgaatcccag ttacggagcc gaagaaaaag ggttaatggc ctcgttgaac tcggtggcaa aattgccgta tacttgggcg ttggacggta tttccaatac gcagtccatt attccggaag cgttgggaca taatgaagaa gaacgggcgg aaaatttggt acatgtattg gacggttact tcgatcaggg cgctcatcac ttaaatgtta atattttcgg aaaagaaaaa ttattggatg ccatggaaca tccggaaaaa ccggaatatg caaattttac gattcgtgta tccggctatg ccgttaaatt tatttcgttg accaaagaac aacagttgga tgttattgca cggacgttcc atgaaaaaag gtaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa

Details for MeloA.20507.a.A1.PW39574

PURIFICATION DATe:	5/14/2026
CONCENTRATION:	27.2mg/ml
OBSERVED MW:	88kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	6
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGTTGGAA AACAAAGGAT GGGAAGGTTT TACAGGAAGA CTTTGGCGAG AAGAGTGTAA TGTTCGGGAT TTTATTCAAA ATAATTATCA GCCGTATGAA GGTGACGAAC GGTTTTTACA AGCGCCTACA GAGGCCACCG ATATATTGTG GGGCAAGGTT CAGGAATATC AAAAAGCGGA ACGGGAACAC GGCGGTGTTT TAAAAGAAGA AACGGAAATC GTTTCCTCTA TTACGGCGTA TGGCCCCGGC TATATTGATC CGCAAATGAA AGAGTTGGAA AAAGTAGTCG GCTTGCAGAC GGATGAACCG TTGAAACGTG CATTCATGCC GTACGGCGGC ATTAAAATGG CGGAAGAAGC CTTGGAAATG TACAATTACA AATTGAATCC GGAACTGCAT AAGGTGTTTA CGGAATATCA TAAAACACAC AATGAAGCGG TTTTTGATGC GTATACTAAG GAAATGTTGC AGGCTCGGCA CACCCATATC ATTACGGGGC TTCCCGATGC GTATGCACGC GGCAGAATTG TCGGCGATTA TCGTCGGGTG GCGTTATACG GGATTGATCA GCTCATTGCC TGGAAGGAAC GGGATAAAGA GTATAATGAT GACGGGATTA TGACGGATAA TGTCATTCGT TTGCGGGAAG AAATCGCCGA ACAGATTAAA GCGTTGAAGC AAATGAAAGA ATTGGCCAAC ATTTACGGCT ACGATATTTC CAAACCGGCA ACCAATGCGC GGGAAGCCGT ACAGTGGTTA TATTTCGGGT ATTTGGCGGC AATTAAGACG CAAAACGGCG CGGCTATGTC GGTAGGTCGT ATTTCGACGT TCCTGGATAT TTATATTGAA CGCGACTTGG CGGCCGGCGA GATTACGGAA CAGGAAGCAC AAGAATTGAT TGACCACTTG ACGCTGAAAT TCCGGATTGT CAAATTCGCC CGCATCCGCT CGTACAATGA ATTGTTCTCG GGCGATCCCA TTTGGGCTAC CTTGGAAATG GCCGGCATCG GCATGGACGG TCGTTCCATG GTAACGAAGA CGGACTTCCG CTTCCTCCAT ACGCTGGAAA ATATGGGACC GGCTCCGGAA CCGAACCTGA CGGTACTGTA TTCGTCGCAG TTGCCGCATC CGTTTAAGAA ATATGCGGCT CATATTTCGG ATACGACGAG CTCCATTCAG TATGAAAACG ATGATGTCAT GAAACCCGTT TGGGGCGATG ACTATTCTAT TTGCTGCTGC GTATCGGCAA CCCAGACAGG GAAAGAAATG CAGTTCTTCG GCGCCCGCGC CAACTTGGCA AAAGCGTTGT TATATGCGAT TAACGGCGGC GTGGATGAAA AATTCGGAAT ACAAGCAGGT CCGGAATTGG CGCCGATTAC TTCGGAATAC TTGGATTATG ATGAAGTCGT ACATAAATAT AAACTCATGC TGGATTGGTT GGCAGGATTG TATGTCAACG TTTTGAATAC CATCCATTAT ATGCATGATA AATATTATTA TGAAGCTGCG GAAATGGCCT TGTTGGATAC GAAACTTCGT CGTACCTTTG CCACCGGGAT TGCCGGGTTC TCGCATGTTA TCGACTCGTT GTCGGCGATT AAGTATGCGA AAGTTAAAGT TATCCGCAAC GATAAGGGCT TAGCGGAAGA CTTCCAAGTT GAAGGCGATT TTCCGAAATA CGGGAATGAT GATGATCGTG CCGATGAAAT CGGTGTAGCT GTTTTGCGGG AATTTATGGA TGATATTCGT AAACGGCATA CGTATCGCGA TTCGGAAGCA ACCACTTCGA TTCTTACGAT TACGTCGAAT GTGGTATACG GCAAATTTAC AGGAAATTTG CCGGACGGTC GTCGGGCGTG GAAACCCTTC GCTCCCGGTG CGAATCCCAG TTACGGAGCC GAAGAAAAAG GGTTAATGGC CTCGTTGAAC TCGGTGGCAA AATTGCCGTA TACTTGGGCG TTGGACGGTA TTTCCAATAC GCAGTCCATT ATTCCGGAAG CGTTGGGACA TAATGAAGAA GAACGGGCGG AAAATTTGGT ACATGTATTG GACGGTTACT TCGATCAGGG CGCTCATCAC TTAAATGTTA ATATTTTCGG AAAAGAAAAA TTATTGGATG CCATGGAACA TCCGGAAAAA CCGGAATATG CAAATTTTAC GATTCGTGTA TCCGGCTATG CCGTTAAATT TATTTCGTTG ACCAAAGAAC AACAGTTGGA TGTTATTGCA CGGACGTTCC ATGAAAAAAG GTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA