MeguA.20306.d

MgAls673 (agglutinin-like sequence) (AIs)

CENTER ID: MeguA.20306.d
ORGANISM: Meyerozyma guilliermondii
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP:
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MeguA.20306.d.B21.GE43744 ALS3-like domain 26 329
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
UniProt: A5DBL8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRHCFSMMLL LFYVCFATVA SAAKLSNVWT SLSITSPQGN RPQDIHSASL GWKIATADAS SGDTFDIIMP GVFRTKFGEQ QLRLVVDDTT YALCDAWDGS YITDYSSLSC TVTSAVNTDR DITATGVATF DFVFGAGGSS RSSDLYEASL YHAGTNTINW SGLSTTVDFV GGPFFNENDN DGLVYYSRST PQLNEQLYVL SASCPAGITS GYTIATFEND YDCSEFSLKM TKDLNDFYLP EGYEDVPSSQ VLCQSGRIVG SFSNVPAGYR VFLQGLYRYP LTSDTVYHNF GGNVQCSSGQ TKTNNYGREI VVITGDSDST GNSKEIIVTT TTWTETYTTV TTLPYESTAS TITIVVEVPD TDRTTTTTTF TSPWTGSFTT TKTLPYETEG PTVTITVEVD VPTSASESSS SSEKSSSIES SSSSLSSSSS SSSVEVSSEV SLATTTTTTT TTWSNPYTST TTLPITFGAT EVTVIVEVPE LTTTTTTTTT TWSNPYTSTT TLPITSGATE VTVIVEVPTG GWSNSSISST QTSSVSKTSI ESSSSLTSEK SEAPECETCT LTSHETVSTT ATIPCEECHK SSSVAETSQT QETTEETQST TAETHKTTET EHCDECEATE TTPLESTETS VPLLTTTTPE TPEEPETTVP EKPETEVPEE PEETTTPEEP EITTIPVEPE ITTDEPEVTD EPEVTISEIP DEPTETETEE TVPETESVEP EETAEPEATT EEESEETLPP QFAVSEFTFE YNTDDFAASP TETETEVPVT EIPITVTSVA AESITACSVS VYSGTASKLV PSLMVILLTL LV
NT Sequence
atgcgtcact gtttctccat gatgttactt ttgttttatg tttgctttgc gacagtagca ctggctgcaa aattgtccaa tgtttggact ctgctttcaa ttaccagccc tcaaggtaat cgtcctcagg atatccattc cgctagcttg gggtggaaga ttgcaactgc cgatgcctct tctggagaca cttttgatat cattatgcct ggagtatttc gtacaaagtt tggtgagcaa cagcttagac tcgttgtaga cgatactact tatgccttgt gtgatgcttg ggatggctcg tatatcaccg attactcgag tcttctgtgt acagtgacta gtgctgtgaa tactgatcgt gacattaccg caaccggagt tgctaccttt gactttgtgt ttggtgccgg tggatcttca agatcttctg acctctacga agccagtctt tatcacgctg gaacaaatac catcaactgg agtggacttt caaccactgt ggattttgtt ggaggaccat ttttcaacga aaatgataat gatggtttgg tctactattc gagatcaact cctcagctta acgaacagtt gtatgttttg tcagcctctt gtccagctgg aatcacctca ggatacacaa ttgctacttt tgaaaacgac tacgactgct ctgaattcag tcttaagatg accaaggatt tgaatgactt ttatcttcct gaaggttatg aagatgtgcc tctgctgcaa gttctttgtc aaagtggtcg tattgttgga agtttctcaa atgtccccgc cggatacaga gtgttcttac aaggattgta cagataccca ttgacctcgg atactgtgta ccacaatttt ggaggaaacg tacaatgtct gtcgggacaa accaaaacca acaattatgg aagagaaatt gttgtcatta ctggtgactc cgactccact ggaaattcaa aggaaatcat agtcaccacc accacttgga ccgaaacata taccaccgtc accacccttc catatgagtc tactgccagt accattacca ttgtggtaga ggttcctgat acagacagaa ctaccaccac tacaacgttt actctgccat ggacgggatc tttcacgacc acaaagacat tgccttatga gacagaaggt ccaacggtga caatcactgt cgaagtcgat gtgcctacgc tggccagcga gagttcttcg agctctgaga aatctagtct gattgagtcg agttccagtt ctttgagttc ttcaagttct tcaagttccg tcgaggtgag ttctgaggtt tctttagcca ctaccacaac aaccacaacc accacttggt ccaatcctta tacttcaacc acaacgttgc caataacttt cggtgccaca gaggtgactg ttattgtaga ggttcctgag cttacaacaa ctaccacaac taccaccacc acctggctga atccatatac ttctaccacg acgcttccaa ttacttcggg agcaactgaa gttacggtta ttgtcgaggt tcctactgga ggatggtcca attccagtat ttcgtcaacg caaacatcta gcgtttcgaa gacatcaatt gagtcttctt caagtttgac cctggagaaa tctgaggccc cagaatgtga gacatgcacc cttacaagcc atgaaactgt ttcgaccaca gctactatcc cttgtgaaga gtgtcacaag tcgctgtcgg tagccgaaac gtcccagact caagagacta ctgaagagac tcaatcgacg acagccgaaa cccataagac aacagaaaca gaacactgtg atgaatgtga agcaactgag accactcctt tggagctgac ggaaacttct gttcctctat tgaccacgac aacgccagaa acaccagaag agcctgaaac aaccgtacca gaaaagcctg aaaccgaagt tcccgaggag cctgaggaaa caacaactcc agaggagcct gagatcacaa ccattccagt agagcctgag ataacaacag atgagcccga ggtgacagat gagcctgagg tgacaatttc ggaaattcca gacgagccaa cagaaacgga gacagaagaa acagttccag aaacagagtc ggtagaacca gaggagacag cagagcccga agcaacaaca gaagaggaat cagaagaaac attgcctcct caatttgctg tttctgaatt cacattcgaa tacaatactg acgattttgc agcaagtcct actgagacag aaaccgaagt tccagtaact gaaattccaa ttactgtaac ttcggttgct gccgaatcta ttactgcctg tagtgtgtct gtgtatagcg gtacagcgtc aaaactcgtc ccctcattga tggtgattct actcacgttg ttagtttag
Details for MeguA.20306.d.B21.GE43744
HARVESTED ON: 7/6/2020
SEQUENCED ON: 6/23/2020
EXPECTED MW: 34kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMS NVWTSLSITS PQGNRPQDIH SASLGWKIAT ADASSGDTFD IIMPGVFRTK FGEQQLRLVV DDTTYALCDA WDGSYITDYS SLSCTVTSAV NTDRDITATG VATFDFVFGA GGSSRSSDLY EASLYHAGTN TINWSGLSTT VDFVGGPFFN ENDNDGLVYY SRSTPQLNEQ LYVLSASCPA GITSGYTIAT FENDYDCSEF SLKMTKDLND FYLPEGYEDV PSSQVLCQSG RIVGSFSNVP AGYRVFLQGL YRYPLTSDTV YHNFGGNVQC SSGQTKTNNY GREIVVITGD SDST
Validated NT Sequence
ccggtggagt cgctgtcacc tgtaataact acgatctcgc gcccgtagtt gtttgtcttt gtctggccag agctgcactg tacattcccg ccgaagttat ggtacactgt gtcagatgta agtgggtaac gatacaatcc ttgcagaaac acgcgatagc ccgctggcac attactgaag gaaccgacaa tgcggccact ttggcacagg acttgacttg atggtacgtc ttcatatcct tccggtaagt aaaaatcgtt aaggtccttg gtcattttta acgagaactc ggagcagtca tagtcgttct cgaacgttgc gattgtgtaa cctgacgtga tacccgccgg gcacgatgcg ctaagaacat acaactgttc gttcagttgt ggcgtggaac gagaataata aacaagtcca tcgttgtcgt tttcattaaa aaaaggacca ccaacaaaat ctacggtcgt cgaaagtccg ctccaattga ttgtattcgt accggcgtgg tataaagatg cctcgtacag gtcagaactg cgcgaagacc ctccggcgcc aaaaacaaaa tcgaatgttg ccaccccggt agctgtaata tcgcggtcgg tgtttacagc actcgtcacg gtacagctca atgacgaata atctgtgatg taactaccat cccaagcatc gcacagggcg tacgtcgtgt catcaacgac caggcgtaac tgctgctccc caaatttagt gcgaaaaacg cccggcataa tgatgtcgaa cgtatcaccc gagcttgcat cagcagttgc gatcttccat cccaacgacg ccgagtgaat atcttgcggg cggtttcctt gtggagatgt aatactaagg gaggtccaaa cgttactcat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHSN VWTSLSITSP QGNRPQDIHS ASLGWKIATA DASSGDTFDI IMPGVFRTKF GEQQLRLVVD DTTYALCDAW DGSYITDYSS LSCTVTSAVN TDRDITATGV ATFDFVFGAG GSSRSSDLYE ASLYHAGTNT INWSGLSTTV DFVGGPFFNE NDNDGLVYYS RSTPQLNEQL YVLSASCPAG ITSGYTIATF ENDYDCSEFS LKMTKDLNDF YLPEGYEDVP SSQVLCQSGR IVGSFSNVPA GYRVFLQGLY RYPLTSDTVY HNFGGNVQCS SGQTKTNNYG REIVVITGDS DSTGNSKEII VT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catagtaacg tttggacctc ccttagtatt acatctccac aaggaaaccg cccgcaagat attcactcgg cgtcgttggg atggaagatc gcaactgctg atgcaagctc gggtgatacg ttcgacatca ttatgccggg cgtttttcgc actaaatttg gggagcagca gttacgcctg gtcgttgatg acacgacgta cgccctgtgc gatgcttggg atggtagtta catcacagat tattcgtcat tgagctgtac cgtgacgagt gctgtaaaca ccgaccgcga tattacagct accggggtgg caacattcga ttttgttttt ggcgccggag ggtcttcgcg cagttctgac ctgtacgagg catctttata ccacgccggt acgaatacaa tcaattggag cggactttcg acgaccgtag attttgttgg tggtcctttt tttaatgaaa acgacaacga tggacttgtt tattattctc gttccacgcc acaactgaac gaacagttgt atgttcttag cgcatcgtgc ccggcgggta tcacgtcagg ttacacaatc gcaacgttcg agaacgacta tgactgctcc gagttctcgt taaaaatgac caaggacctt aacgattttt acttaccgga aggatatgaa gacgtaccat caagtcaagt cctgtgccaa agtggccgca ttgtcggttc cttcagtaat gtgccagcgg gctatcgcgt gtttctgcaa ggattgtatc gttacccact tacatctgac acagtgtacc ataacttcgg cgggaatgta cagtgcagct ctggccagac aaagacaaac aactacgggc gcgagatcgt agttattaca ggtgacagcg actccaccgg gaacagtaag gagattattg ttacttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat