MeguA.20306.c

MgAls3300 (agglutinin-like sequence) (AIs)

CENTER ID: MeguA.20306.c
ORGANISM: Meyerozyma guilliermondii
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP:
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MeguA.20306.c.B21.GE43743 ALS3-like domain 20 333
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
UniProt: A5DJ79

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTPLFLFTLW AAVASAAQVS GVFTGFPSLV WQAGGNYYKP LPEFSSWVAT LDWAIQGSKF KAGDTFTLHM PCVFQFTANT PTVQLKVNND VYATCNFYSG EIITTYSELQ CTMSDLVNDQ TDVSGTLTVP MFFNTGLTAN AVDLTNAKCF KAGDNTVTFT DGNNEISTNV NFNGGQMLGP LDPNNIIVGS RFVPSLNTLQ HFLAAGKCDN GYISGTLGIT VDNTNLDCST LQAQITSQLN DWFFPESSQN FNFQTTCTGN TFTATYNNVP AGFRPFIDIM GSAPSGNNGF IVHYTNDYFC NGDPTVHSNG QGVNWGQPQN TDSGSDGNAA VVTTRTWTGS TTRVTTLPYN TAPGNTKTIE VDVPIPTTTV TSTWTGTNTV TTTKTATPGG TATVVVDVPT PATTITSTWT GSFSTTSTIT APSGGTETVV VDVPTPTTTI TKTWTGTETT TTTITAPPGG TNTVVVDVPT PRTTITSTWT GTNTTTVTVP PASSGGTETV VVEVPTPTTT VTKTWTGTET TTTTITAPPG GTNTVVVDVP TPATTITSTW TGTNTTTVTI PPATSGGTET VVVEVPTPTT TITKTWTGTE TTTTTITAPP GGTNTVVVDV PTPATTITST WTGTNTTTVT IPPASSGGTE TVVVEVPTPT TTITKTWTGT ETTTTTITAP PGGTNTVVVD VPTPATTITS TWTGTNTTTA TVPPASSGGT ETVVVEVPTP TTTITKTWTG TETTTTTITA PPGGTNTVVV DVPTPATTIT STWTGTNTTT VTVPPASSGG TETVVVEVPT PTTTITKTWT GTETTTTTIT APPGGTNTVV VDVPTPATTI TSTWTGTNTT TVTLPPASSG GTETVVVEVP TPTTTITKTW TGTETTTTTI TAPPGGTNTV VVDVPTSRIS EPPSSSSELS SSSLSSSGFS TLSVSSVSSF SSSESLSSSY IPPFSNTSVP TVSSSSLTSS SSVLISSTSV SLTSGVPSSS SEVPPTSVPT GPSGPSSSEG PSSSSEVPST SVPTGPSELS SSEVPSSSVP TGPSGPSSSE GPSSSSEVPP TSVPTGPTGP SSSVPTGPSV PTGPTEPTGP TSEPTVPTGP SVPTGPTSEP TGPTEPTGPT GPTGPSGPSV PTGPTGPTVP TGPTSEPTVP TGPSVPTGPT SEPTVPTGPS VPTGPTEPTG PTEPTGPTGP SVPTEPTEPT GPTEPTEPTG PSVPTGPTGP SVPTGPTGPS VPTGPSGPNV PTGPTEPTGP SVPTGPSEPS EPTGPNGPSE PNVPTGPSNP SEPTTTVIVP PAGTVPTDDT NVPPATTGTP TTTSGTPIEP TTSGGNGGND GNDGNGGNGG NGGNGGNGGD NGNGGNTPSG NFPGVSSTVS YYSPPQSASV STYEGSASSF SFPLGAMGVI LLSYII
NT Sequence
atgaccccat tatttctttt caccttgtgg gcagccgtag cctcggcagc tcaggtgtct ggagtattta ctgggtttcc gagtttagtt tggcaagctg gtggcaacta ctataagcca ttgcccgaat tttccagttg ggttgccaca cttgactggg ctattcaagg ttccaagttc aaggcaggcg acacgtttac tttgcacatg ccatgcgtct tccaattcac cgctaatacc ccaactgtac aattgaaagt gaataatgac gtttatgcga cgtgtaattt ctactccgga gaaatcatta ccacttattc cgagcttcaa tgtaccatgt ccgaccttgt gaatgaccaa accgacgtct ctggtactct taccgtgccc atgttcttta atactggtct aactgccaat gcggttgatc ttaccaacgc caagtgtttc aaggctggtg acaataccgt gacttttact gatggaaaca acgagatcag tacaaatgtt aacttcaatg gcggtcaaat gttagggccc cttgacccta acaacattat tgtgggctcc agatttgttc cctctctcaa tacgctccag cattttttag cggcgggaaa atgtgacaat ggttatatca gtggtacgtt gggaatcacc gtcgacaaca caaacttgga ctgcagcact ttacaagctc aaatcactct gcaactcaac gactggttct tccctgaaag ctcgcagaac ttcaactttc aaaccacttg tactggtaac accttcaccg ccacctataa caatgtgcct gctggattcc gtccctttat cgacatcatg ggatctgctc cttcaggcaa caacggattt attgtgcact acaccaacga ctacttctgt aacggagacc caaccgtaca cagtaacgga cagggagtga attggggtca gccccagaat actgacagtg gttctgacgg taacgcagct gtggttacca caagaacatg gactggttca acaaccagag tcactacttt gccatacaac accgctcctg gtaacaccaa aaccatcgag gttgatgttc ctattccaac tactactgtc accagtacct ggactggaac caatactgtt accaccacca agactgctac tcctggtgga accgctacag tagtagttga tgtacctact ccagcaacta caatcacttc tacttggaca ggttcatttt ctacgacgag tacgattact gctccttctg gtggaactga aacagtagtt gtggatgtgc caacgccaac tacaactatt accaagactt ggacgggaac tgaaaccacc actactacca ttactgctcc acctggtgga actaacacag tagtagtcga tgtacctact ccaagaacta caattaccag cacttggact ggtactaaca ccaccactgt gactgttcct ccagcttcct ctggtggaac cgagacagtg gtcgttgaag ttccaactcc aactaccaca gtaacaaaaa cgtggaccgg aactgaaact actactacta ccattactgc tcctcctggc ggtaccaaca cagtagtagt tgatgtacct actcctgcta ccaccatcac tagcacctgg accggaacta acactactac tgttactatc cctccagcta cttctggtgg aactgagaca gtggttgttg aggtaccaac tcctacgact acaatcacta agacttggac cggaaccgaa accacaacta ctaccattac tgctcctcca ggaggaacca acacagtagt ggttgatgtt ccaactcccg ccaccaccat cacaagtacc tggactggga ctaataccac gactgtcact atccctccag cttcatctgg tggaactgag acagtcgttg ttgaagttcc tacccctacc accaccatta ccaagacttg gactggaact gagaccacta ctacaactat tactgctcct cctggtggaa ctaatactgt ggtagttgat gttcctactc ccgctactac catcactagt acctggactg gtaccaatac cacaactgct accgttcctc cagcttcatc cggaggcact gaaacagtgg ttgttgaggt tccaactcct acgaccacta tcactaagac ttggaccgga actgaaacca caactacaac tatcaccgct cctccaggtg gaactaatac tgtggtagtt gatgttccta ctcctgctac caccatcact agcacttgga ctggcactaa caccaccact gtgactgttc ctccagcttc ttccggtgga actgagacag tagtcgttga agttccaacc ccaaccacaa ccattaccaa gacttggact ggtaccgaga ccactactac aactattact gctcctcctg gtggaactaa tactgtggta gttgatgtcc ccactcccgc aactacaatc acaagtacct ggactggcac taacaccaca actgttaccc ttcctccagc ttcttccggt ggaactgaga cagtggttgt tgaggtacca accccaacca caaccattac caagacttgg actggtaccg agaccactac tacaactatc accgctcctc ctggtggaac taatactgtg gtagttgatg ttccaacatc gagaatttcc gagcctcctt cgagttcttc agaactttcc agttcatctt tgagctcgtc cggattctcg acactttctg tctcgtctgt ttcgtcattt tcttcatcgg aatcattgag ctcttcatac attcctccat tttcgaatac gtccgtccct acggtttcaa gttcatcatt gacctcgtct tcgagtgtct tgatcagctc aacatccgta tcccttactt ccggggtacc atcttcctca tctgaagttc ctccaacatc agtgccaact ggacctagtg gaccatcttc ttctgaggga ccatcttcgt catctgaagt tccatcaaca tctgttccaa ccggaccttc tgaactttca tcttctgagg taccatcatc ctcagttcca accggacctt ctggaccttc ttcttctgag ggtccatctt cctcatctga agttccacca acatcggtac caactggacc aactggaccc tcttcttctg ttccaactgg acctagtgtt ccaactggcc caactgaacc taccggccca accagtgagc caactgttcc tactggacct tctgttccaa cgggcccaac tagtgaacca acaggaccca ctgaacctac tggacctacg ggaccaactg gaccttctgg acctagcgtt ccaactggtc ctactggtcc tactgttcca actggcccaa ctagtgagcc aactgttcct accggacctt ctgttccaac tggcccaact agtgagccaa ctgttcctac cggaccttct gttccaactg gacctaccga gcctactgga ccaactgaac ccactggtcc tactggacct tctgttccaa ctgaacctac cgagcctact ggaccaactg aacccactga gcctactgga ccttctgttc caactggacc cactggacct tctgttccaa ctggacccac tggaccttct gttccaactg gaccttctgg gccaaacgtt ccaactggtc ccaccgagcc cactggacct tctgttccaa ccggaccatc tgaaccatct gaacctactg gacctaatgg accatcagaa cccaatgtcc caactggacc ttctaatcct tcagaaccta ctactactgt tatagtgcct cctgctggaa ccgtgcctac cgacgatact aacgtgcctc cagccacaac tggaacacca accacaactt ccggaacacc cattgagcca accacttcag gtggaaacgg tggaaatgat ggaaatgatg gaaatggtgg aaatggtggg aatggtggga atggtggtaa tggaggtgat aatggaaacg gtggaaacac cccatctgga aacttccctg gcgtttcgtc tacagtctca tactattccc cacctcaaag tgcaagcgtc tccacctatg aaggaagcgc ttcaagcttc tcgttcccat tgggagcgat gggtgtcatt ttactttcgt acattatttg a
Details for MeguA.20306.c.B21.GE43743
HARVESTED ON: 7/6/2020
SEQUENCED ON: 6/23/2020
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 71
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMS GVFTGFPSLV WQAGGNYYKP LPEFSSWVAT LDWAIQGSKF KAGDTFTLHM PCVFQFTANT PTVQLKVNND VYATCNFYSG EIITTYSELQ CTMSDLVNDQ TDVSGTLTVP MFFXTGLTAN AVDLTNAKCF KAGDNTVTFT DGNNEISTNV NFNGGQMLXP LDPNNIIVGS RFVPSLNTLQ HFLXAGKCDN GYISGTLGNX RRQHKLGLQH FTXSNHSATX XLXLXLKARR TSTFKPLVXX XXXXPPITXX XXXSVXXXXS WDXXXQATXX XXCXTPXXTX VTETXXYTVT XREXXGVSXX XXXX
Validated NT Sequence
annnnnngnn antannnngn ggctgacccc nnttcnctcc ctgnncgtta ctgtgtacng ntgggtctcc gttacanaag tagncnntgg ngtantgcac annnnntnng ntgttgcctg anggancana tcccatgatg ncnannnnng gacggantcc nncnngnncn ttgttatagg tggnnnnnan ngngnnnnnn nnacaagtgg tttgaaagtt gaagttctgc gagctttcag ggnaagnncc agncnntgag ttgcagagtg atttgagntn gtaaagtgct gcagtccaag tttgtgttgt cgacgnngat tccccaacgt accactgata taaccattgt cacattttcc cgccnntaaa aaatgctgga gcgtattgag agagggaaca aatctggagc ccacaataat gttgttaggg tcaaggggcc ntaacatttg accgccattg aagttaacat ttgtactgat ctcgttgttt ccatcagtaa aagtcacggt attgtcacca gccttgaaac acttggcgtt ggtaagatca accgcattgg cagttagacc agtannaaag aacatgggca cggtaagagt accagagacg tcggtttggt cattcacaag gtcggacatg gtacattgaa gctcggaata agtggtaatg atttctccgg agtagaaatt acacgtcgca taaacgtcat tattcacttt caattgtaca gttggggtat tagcggtgaa ttggaagacg catggcatgt gcaaagtaaa cgtgtcgcct gccttgaact tggaaccttg aatagcccag tcaagtgtgg caacccaact ggaaaattcg ggcaatggct tatagtagtt gccaccagct tgccaaacta aactcggaaa cccagtaaat actccagaca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHSG VFTGFPSLVW QAGGNYYKPL PEFSSWVATL DWAIQGSKFK AGDTFTLHMP CVFQFTANTP TVQLKVNNDV YATCNFYSGE IITTYSELQC TMSDLVNDQT DVSGTLTVPM FFNTGLTANA VDLTNAKCFK AGDNTVTFTD GNNEISTNVN FNGGQMLGPL DPNNIIVGSR FVPSLNTLQH FLAAGKCDNG YISGTLGITV DNTNLDCSTL QAQITLQLND WFFPESSQNF NFQTTCTGNT FTATYNNVPA GFRPFIDIMG SAPSGNNGFI VHYTNDYFCN GDPTVHSNGQ GVNWGQPQNT DSGSDGNAAV VT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattctggag tatttactgg gtttccgagt ttagtttggc aagctggtgg caactactat aagccattgc ccgaattttc cagttgggtt gccacacttg actgggctat tcaaggttcc aagttcaagg caggcgacac gtttactttg cacatgccat gcgtcttcca attcaccgct aataccccaa ctgtacaatt gaaagtgaat aatgacgttt atgcgacgtg taatttctac tccggagaaa tcattaccac ttattccgag cttcaatgta ccatgtccga ccttgtgaat gaccaaaccg acgtctctgg tactcttacc gtgcccatgt tctttaatac tggtctaact gccaatgcgg ttgatcttac caacgccaag tgtttcaagg ctggtgacaa taccgtgact tttactgatg gaaacaacga gatcagtaca aatgttaact tcaatggcgg tcaaatgtta gggccccttg accctaacaa cattattgtg ggctccagat ttgttccctc tctcaatacg ctccagcatt ttttagcggc gggaaaatgt gacaatggtt atatcagtgg tacgttggga atcaccgtcg acaacacaaa cttggactgc agcactttac aagctcaaat cactctgcaa ctcaacgact ggttcttccc tgaaagctcg cagaacttca actttcaaac cacttgtact ggtaacacct tcaccgccac ctataacaat gtgcctgctg gattccgtcc ctttatcgac atcatgggat ctgctccttc aggcaacaac ggatttattg tgcactacac caacgactac ttctgtaacg gagacccaac cgtacacagt aacggacagg gagtgaattg gggtcagccc cagaatactg acagtggttc tgacggtaac gcagctgtgg ttacctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat