MasyA.00765.a

Flavohemoglobin; nitric oxide oxidoreductase; yeast protein YHB1 ortholog

CENTER ID: MasyA.00765.a
ORGANISM: Malassezia sympodialis ATCC 42132
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MasyA.00765.a.TH11.PD38341 full length 2 413

External Resources

RESOURCE REFERENCE ID
EuPathDB: FungiDB:MSYG_3741
UniProt: M5E7R5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFVNHIDECR GQLSPEHGEV IKATLPLVGA NIENITKLFY KTMFEKHPEL IRNVFNRGNQ KQGAQQKALA ASVATFASML VNKDAPLPDH LLSRIGHKHV SLGVTEDQYQ IVHDNLFYAI VQVLGADVVT KDVAEAWDSV YWIMARLLIN FEKDLYKGAK VEPGKVFRQT TVLEREALTS DVVRFTIESK DGSKPLPAHL PGQYISVRAK LPDGAGQLRQ YSLVDSGEKP GRLSFAVKAL EASESAPAGE VSNWLVDNAK VGTDLEVSVP FGDLVLNTES NAPVVLISAG IGATPMIGML SRLSSVASER HVVIVHADTN SGTDAFATHR EHLASALKNY ELHVFYKEGA SGKNVTIGHL DVSKFAFPND AEFYLCGSTT FLQEAREGLK ANNVKPSSVH FELFMPNDWL LDE
NT Sequence
atgtttgtga accatattga tgaatgccga gggcagcttt ctccagaaca cggtgaagtg atcaaggcca cgcttcctct tgtgggtgct aacattgaga acatcacaaa attgttctac aagactatgt tcgagaaaca tccagagctc attaggaatg tgttcaaccg tggtaaccaa aaacaaggtg cacagcagaa agcactcgct gcttcagtgg ctacgttcgc ctcgatgctg gtgaacaaag atgcgccgct tcctgaccac ctactgtctc gcattggcca caagcacgtg tcattgggtg tgacagagga tcagtaccaa attgttcacg acaatctttt ctatgcaatt gttcaggtac ttggtgctga tgtagtgacg aaggatgtgg cagaggcatg ggactctgta tactggatta tggcgcgtct attgatcaac ttcgagaagg acctctataa gggtgctaaa gtggaacctg gcaaagtgtt ccgccaaact accgtgctgg agcgtgaggc ccttactagt gatgtggtcc gcttcactat tgaaagtaag gacggctcga agccccttcc tgctcactta cctggtcagt acatttctgt ccgtgcgaaa ctccctgatg gtgctggcca gctgcgccag tacagtctgg tcgattcggg ggagaagcct ggtcgtctaa gctttgctgt gaaggcgctg gaagcgtccg agtcagcacc tgctggcgaa gtttccaact ggcttgtgga taacgcaaaa gtgggcaccg atctggaggt gtcagtgccg ttcggtgatt tggtgctcaa cacagagagc aatgcgccag tggttctgat ctccgctggc attggtgcga ccccgatgat tggcatgctt tcccgtctgt cgtcagttgc ctcagaacgc catgtcgtta ttgtgcatgc ggataccaat tcgggtactg acgcctttgc gactcatcgg gagcacttgg catcagcttt gaagaactat gaattacacg ttttctacaa ggaaggtgcc tctggcaaaa atgtcactat tggccacttg gatgtaagca aattcgcttt cccgaatgac gccgagtttt acttgtgtgg ctctaccact ttccttcagg aagctcgcga aggtctgaag gccaacaatg tgaagccgag ctcggtccac ttcgagctgt ttatgcctaa cgactggctt cttgacgaat aa
Details for MasyA.00765.a.TH11.PD38341
PURIFICATION DATe: 11/14/2018
CONCENTRATION: 20.52mg/ml
OBSERVED MW: 48kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 10mM HEPES pH 7.5, 150mM NaCl
EXPRESSION HOST: BL21 DE3
VIAL COUNT (approx.): 27
VIAL VOLUME: 100µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MGHHHHHHHH ENLYFQGSGF VNHIDECRGQ LSPEHGEVIK ATLPLVGANI ENITKLFYKT MFEKHPELIR NVFNRGNQKQ GAQQKALAAS VATFASMLVN KDAPLPDHLL SRIGHKHVSL GVTEDQYQIV HDNLFYAIVQ VLGADVVTKD VAEAWDSVYW IMARLLINFE KDLYKGAKVE PGKVFRQTTV LEREALTSDV VRFTIESKDG SKPLPAHLPG QYISVRAKLP DGAGQLRQYS LVDSGEKPGR LSFAVKALEA SESAPAGEVS NWLVDNAKVG TDLEVSVPFG DLVLNTESNA PVVLISAGIG ATPMIGMLSR LSSVASERHV VIVHADTNSG TDAFATHREH LASALKNYEL HVFYKEGASG KNVTIGHLDV SKFAFPNDAE FYLCGSTTFL QEAREGLKAN NVKPSSVHFE LFMPNDWLLD E
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCCATC ACCACCATCA CCACCATCAC GAAAATCTGT ATTTCCAGGG TAGCGGTTTT GTGAACCATA TTGATGAATG CCGAGGGCAG CTTTCTCCAG AACACGGTGA AGTGATCAAG GCCACGCTTC CTCTTGT GGGTGCTAAC ATTGAGAACA TCACAAAATT GTTCTACAAG ACTATGTTCG AGAAACATCC AGAGCTCATT AGGAATGTGT TCAACCGTGG TAACCAAAAA CAAGGTGCAC AGCAGAAAGC ACTCGCTGCT TCAGTGGCTA CGTTCGCCTC GATGCTGGTG AACAAAGATG CGCCGCTTCC TGACCACCTA CTGTCTCGCA TTGGCCACAA GCACGTGTCA TTGGGTGTGA CAGAGGATCA GTACCAAATT GTTCACGACA ATCTTTTCTA TGCAATTGTT CAGGTACTTG GTGCTGATGT AGTGACGAAG GATGTGGCAG AGGCATGGGA CTCTGTATAC TGGATTATGG CGCGTCTATT GATCAACTTC GAGAAGGACC TCTATAAGGG TGCTAAAGTG GAACCTGGCA AAGTGTTCCG CCAAACTACC GTGCTGGAGC GTGAGGCCCT TACTAGTGAT GTGGTCCGCT TCACTATTGA AAGTAAGGAC GGCTCGAAGC CCCTTCCTGC TCACTTACCT GGTCAGTACA TTTCTGTCCG TGCGAAACTC CCTGATGGTG CTGGCCAGCT GCGCCAGTAC AGTCTGGTCG ATTCGGGGGA GAAGCCTGGT CGTCTAAGCT TTGCTGTGAA GGCGCTGGAA GCGTCCGAGT CAGCACCTGC TGGCGAAGTT TCCAACTGGC TTGTGGATAA CGCAAAAGTG GGCACCGATC TGGAGGTGTC AGTGCCGTTC GGTGATTTGG TGCTCAACAC AGAGAGCAAT GCGCCAGTGG TTCTGATCTC CGCTGGCATT GGTGCGACCC CGATGATTGG CATGCTTTCC CGTCTGTCGT CAGTTGCCTC AGAACGCCAT GTCGTTATTG TGCATGCGGA TACCAATTCG GGTACTGACG CCTTTGCGAC TCATCGGGAG CACTTGGCAT CAGCTTTGAA GAACTATGAA TTACACGTTT TCTACAAGGA AGGTGCCTCT GGCAAAAATG TCACTATTGG CCACTTGGAT GTAAGCAAAT TCGCTTTCCC GAATGACGCC GAGTTTTACT TGTGTGGCTC TACCACTTTC CTTCAGGAAG CTCGCGAAGG TCTGAAGGCC AACAATGTGA AGCCGAGCTC GGTCCACTTC GAGCTGTTTA TGCCTAACGA CTGGCTTCTT GACGAATGAT AAGCTTGCGG CCGCACTCGA GCACCACCAC CACCACCACT GAGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGAT