LpinB.19562.a

Putative lipoprotein

CENTER ID: LpinB.19562.a
ORGANISM: Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpinB.19562.a.B1.GE41912 full length 1 365
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|189518.3.peg.3005
UniProt: Q8F1V2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKEFLQKTKR GASVLLIMYV IWNCGGEKQN DSSSLLMLLG PEQSLQTETY SMTSDQNIGV NQNQPELNPG SEEVVGYSNS LEPDLMVYVY FPGSRQMKPG EDISRKLSLQ VANFGGSLVA GNRPDQEGYM VDLILSKDKE VPEGYASYSS DFQEDVLLGG GRISNTPDLV SGALKYVSEG SNILPKNIPT GDYYLCARVD IGSKIAESNE SNNTNCFPIS IQMEEALPDL IIPRASVYPS GMKCRAHKPM MFITAEIKNI GQGPSPVMSN VGIINALEPD GIRGNGIGYE SVIQPGETVT VTFPIYFPIN ESLEVDTSLV EKKHTFDLRL NRGNWLPESN VQNNAYTRKP LELTIPEGYC KSHSD
NT Sequence
atgaaagaat tcttacaaaa gacgaagaga ggggcgagcg ttctcctgat aatgtatgtg atttggaatt gtggaggaga aaaacaaaat gattcttcct ctttgttaat gcttttggga cctgagcagt ctttgcagac tgagacttat tctatgactt cagaccaaaa tataggtgta aatcaaaatc aaccagaact caatcctgga tctgaagaag tagtcggtta cagtaattct ttggaacccg atcttatggt ttatgtttat ttccctggct caagacaaat gaaaccggga gaagacatct ctcgcaaact aagccttcaa gttgctaatt ttggtggaag tttggtggct ggaaatcgtc ccgatcagga aggttatatg gtagatttga ttctttctaa agataaagag gtgcccgaag gttatgcttc ttattcttcc gattttcaag aagacgtttt gttaggcggt ggaagaatta gtaacactcc ggacctggtt tcaggtgctc tcaaatatgt aagcgaagga tctaatattc ttcctaaaaa cataccaact ggagattatt atctttgtgc gagagtcgat ataggatcta aaattgcaga atcgaatgaa agtaataata cgaattgttt tccaatttcg attcaaatgg aagaggctct tcctgattta attatcccac gtgcttctgt ttatcctagt ggaatgaaat gtagagctca taaaccgatg atgtttatca ctgctgaaat caagaatata ggacaaggtc caagcccggt gatgtcaaat gtaggtatca tcaacgcatt agaaccggat ggaattcgtg gaaatggaat tggatacgaa tctgtaatcc aacctggaga aaccgtcacg gttacatttc caatttattt tccaattaac gaatctctcg aagtggatac ttctttggtt gaaaagaaac atacatttga cctgagatta aatcgtggaa attggctgcc agaatcgaat gtgcaaaaca acgcatatac tagaaaacca ttagaactta cgattccaga aggttattgt aaaagtcatt ctgattga
Details for LpinB.19562.a.B1.GE41912
HARVESTED ON: data unavailable
SEQUENCED ON: 9/29/2017
EXPECTED MW: 41kDa
OBSERVED MW: 86kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMK EFLQKTKRGA SVLLIMYVIW NCGGEKQNDS SSLLMLLGPE QSLQTETYSM TSDQNIGVNQ NQPELNPGSE EVVGYSNSLE PDLMVYVYFP GSRQMKPGED ISRKLSLQVA NFGGSLVAGN RPDQEGYMVD LILSKDKEVP EGYASYSSDF QEDVLLGGGR ISNTPDLVSG ALKYVSEGSN ILPKNIPTGD YYLCARVDIG SKIAESNESN NTNCFPISIQ MEEALPDLII PRASVYPSGM KCRAHKPMMF ITAEIKNIGQ GPSPVMSNVG IINALEPDGI RGNGIGYESV IQPGETVTVT FPIYFPINES LEVDTSLVEK KHTFDLRLNR GNWLPESNVQ NNAYTRKPLE LTIPEGYCKS HSD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaag aattcttaca aaagacgaag agaggggcga gcgttctcct gataatgtat gtgatttgga attgtggagg agaaaaacaa aatgattctt cctctttgtt aatgcttttg ggacctgagc agtctttgca gactgagact tattctatga cttcagacca aaatataggt gtaaatcaaa atcaaccaga actcaatcct ggatctgaag aagtagtcgg ttacagtaat tctttggaac ccgatcttat ggtttatgtt tatttccctg gctcaagaca aatgaaaccg ggagaagaca tctctcgcaa actaagcctt caagttgcta attttggtgg aagtttggtg gctggaaatc gtcccgatca ggaaggttat atggtagatt tgattctttc taaagataaa gaggtgcccg aaggttatgc ttcttattct tccgattttc aagaagacgt tttgttaggc ggtggaagaa ttagtaacac tccggacctg gtttcaggtg ctctcaaata tgtaagcgaa ggatctaata ttcttcctaa aaacatacca actggagatt attatctttg tgcgagagtc gatataggat ctaaaattgc agaatcgaat gaaagtaata atacgaattg ttttccaatt tcgattcaaa tggaagaggc tcttcctgat ttaattatcc cacgtgcttc tgtttatcct agtggaatga aatgtagagc tcataaaccg atgatgttta tcactgctga aatcaagaat ataggacaag gtccaagccc ggtgatgtca aatgtaggta tcatcaacgc attagaaccg gatggaattc gtggaaatgg aattggatac gaatctgtaa tccaacctgg agaaaccgtc acggttacat ttccaattta ttttccaatt aacgaatctc tcgaagtgga tacttctttg gttgaaaaga aacatacatt tgacctgaga ttaaatcgtg gaaattggct gccagaatcg aatgtgcaaa acaacgcata tactagaaaa ccattagaac ttacgattcc agaaggttat tgtaaaagtc attctgattg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat