LpinB.19553.c

Thermolysin

CENTER ID: LpinB.19553.c
ORGANISM: Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpinB.19553.c.B1.GE41911 full length 1 753
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|189518.3.peg.4135
UniProt: Q8EYP9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MWVLNPVQLD NKALSERTYT SDGRITFAKF NSDLVPYSRK QAPEVLKTYL QLPLTSQATF LRSARVGDFD HDRFQQRYKG VRVENGIYTV VSKENTIELM MGEFYQVPEN FNSSPKLSES EALSKTLKHI GAKKYIWESV EREAALKRKK NDPNASYFPK GELLVYEHSL GKSNTKKEFR LAYKFGIASI DPPISRYVYV DSNSGEILSS KDARRYEGVQ FPSPKPPIEI DIDYSRCIID KEYCIEQGTA MTRFSGLQSI TTWTAGKTNH FELKDNSRGG GIYTYSWEFV KDPLEDIQLL NIPMIDTNNS WSQSEYHDNY NHDALLDAHW GIEMTYDYFK SIHNRLSYDG DNSRIFNNVH YFNAFVANNA YWDPVTEEIY YVYCPHKNSI CKGFNLPILL DPKYEDFTSL DIVSHEFGHG INGDLAGFNL DMEPGALDEG FSDIWNVGVN NYVNKVLGMQ KNIWLVGDET VPGGGMRSVS NPKSTTVMSP GPNTYHGDLW DFEDNEAHTN SLVLSHWFYT LSKGKQGFND YECTYNVSGI GIEKAERIAY VALLFLSSTS GYTSARTYAI IAAKLLYGLF SSEVKSTIDA WDAVAVPAET TSRGGQGMVR PRHYIASVKL SNVTNDSGND CGYKDNSYLL PTVLRGVTYN MVLLSQGSAS NPSKVHKWRV WIDFNQNGSF ESSEMVVQDT VNSSFGGTLQ KSIKIPTNAL TGYAKMRVSM KAAQSGEAYQ GSSESFVEGE VEDYIVSILD FSL
NT Sequence
ttgtgggttt taaatcctgt ccaattggat aataaggcgc tttctgaaag gacgtatacg tcagatggta ggataacatt tgcgaagttc aattcggatc tagttcctta ttcacgtaaa caagcacctg aagttcttaa gacgtattta caattaccct taacttctca agctacattc ttgcgttcgg ctcgtgtagg agattttgat cacgatcgtt ttcaacagag atataaaggt gttagagtag agaatggaat ttatacagtg gtctcaaaag agaatacgat agaactgatg atgggtgaat tttaccaagt tccagagaat tttaattctt ctcctaaact atccgagtca gaagcacttt caaagacttt aaaacatatc ggagctaaga agtatatttg ggaatctgtt gaacgagaag cagcattgaa acgtaagaag aatgatccaa atgcaagtta ttttccgaag ggagaactat tagtttatga acattcatta ggaaaatcca atactaaaaa agagtttcga ttggcttata aatttggtat agcctcgata gatccaccaa tatcgagata cgtgtatgtg gattctaact ctggggaaat tctatccagt aaagatgcta gaagatacga gggagttcaa tttccaagtc caaaacctcc tatagaaata gatattgatt atagtcgttg tataatagac aaagaatatt gtatcgaaca gggaacggct atgacccgtt ttagcggatt acaatcgatt acaacttgga cggcaggaaa aacgaaccat tttgaactga aagataattc gagaggcggt ggaatttata cttactcttg ggaatttgta aaagatcctt tggaagatat tcaacttttg aatattccaa tgattgatac taataactca tggtcgcaat ctgaatatca tgacaactac aatcatgatg cgttattgga tgcacattgg ggaattgaaa tgacttatga ctatttcaaa tccattcata atcggttgag ttatgatggt gataatagta gaatatttaa taacgtgcac tattttaatg cgtttgtagc gaataacgcg tattgggatc ctgttacgga agaaatatat tacgtttact gtcctcataa aaatagtata tgcaagggtt ttaatctacc gattctctta gaccctaaat atgaagattt tacttctttg gatattgtat ctcatgaatt tgggcatggt atcaacgggg atcttgcggg ttttaatctt gatatggagc caggtgctct cgatgaaggt tttagcgata tttggaatgt tggtgtaaat aactacgtaa ataaagtttt aggaatgcaa aagaatattt ggttagtagg agatgaaacg gttccgggag gaggaatgcg ttctgtgtca aatccaaaat ctactacagt aatgtcccct gggccaaata cgtatcatgg agatctttgg gattttgaag ataacgaagc acatacgaat agtcttgttt tgagtcactg gttctatact ttatccaagg gaaaacaagg atttaacgat tatgaatgca cgtataacgt ttctggaatt ggcattgaaa aagccgaaag aattgcttat gttgcccttc tttttctttc ttctacgtcc ggttatactt ctgcaaggac ttatgcgatt attgcggcta aactattgta tggtttgttt tcttcggaag taaaaagtac aattgatgct tgggatgcag tagctgttcc ggctgagacg acttctcgcg gaggacaagg tatggtaagg cctcgacatt atatcgcttc tgtaaaactt tctaatgtaa caaacgattc cggaaacgat tgtggataca aggataattc atacttactt ccaacggtct tgagaggagt gacgtataac atggtgttat tgagtcaggg ttcggcaagt aatccgtcaa aagttcacaa atggagagta tggatcgatt ttaatcaaaa cggtagtttt gaatcgtcgg aaatggtagt tcaggatact gttaattctt cgtttggagg aacattacaa aaatcgatta agatcccaac gaatgcactt actggatatg cgaagatgag agtttctatg aaggcggctc aaagtggaga agcttatcaa ggttcgagtg aaagctttgt agaaggggaa gtggaggatt atattgtttc gattctagat ttcagtttat ag
Details for LpinB.19553.c.B1.GE41911
HARVESTED ON: 8/31/2017
SEQUENCED ON: 9/29/2017
EXPECTED MW: 86kDa
OBSERVED MW: 86kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMW VLNPVQLDNK ALSERTYTSD GRITFAKFNS DLVPYSRKQA PEVLKTYLQL PLTSQATFLR SARVGDFDHD RFQQRYKGVR VENGIYTVVS KENTIELMMG EFYQVPENFN SSPKLSESEA LSKTLKHIGA KKYIWESVER EAALKRKKND PNASYFPKGE LLVYEHSLGK SNTKKEFRLA YKFGIASIDP PISRYVYVDS NSGEILSSKD ARRYEGVQFP SPKPPIEIDI DYSRCIIDKE YCIEQGTAMT RFSGLQSITT WTAGKTNHFE LKDNSRGGGI YTYSWEFVKD PLEDIQLLNI PMIDTNNSWS QSEYHDNYNH DALLDAHWGI EMTYDYFKSI HNRLSYDGDN SRIFNNVHYF NAFVANNAYW DPVTEEIYYV YCPHKNSICK GFNLPILLDP KYEDFTSLDI VSHEFGHGIN GDLAGFNLDM EPGALDEGFS DIWNVGVNNY VNKVLGMQKN IWLVGDETVP GGGMRSVSNP KSTTVMSPGP NTYHGDLWDF EDNEAHTNSL VLSHWFYTLS KGKQGFNDYE CTYNVSGIGI EKAERIAYVA LLFLSSTSGY TSARTYAIIA AKLLYGLFSS EVKSTIDAWD AVAVPAETTS RGGQGMVRPR HYIASVKLSN VTNDSGNDCG YKDNSYLLPT VLRGVTYNMV LLSQGSASNP SKVHKWRVWI DFNQNGSFES SEMVVQDTVN SSFGGTLQKS IKIPTNALTG YAKMRVSMKA AQSGEAYQGS SESFVEGEVE DYIVSILDFS L
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgtggg ttttaaatcc tgtccaattg gataataagg cgctttctga aaggacgtat acgtcagatg gtaggataac atttgcgaag ttcaattcgg atctagttcc ttattcacgt aaacaagcac ctgaagttct taagacgtat ttacaattac ccttaacttc tcaagctaca ttcttgcgtt cggctcgtgt aggagatttt gatcacgatc gttttcaaca gagatataaa ggtgttagag tagagaatgg aatttataca gtggtctcaa aagagaatac gatagaactg atgatgggtg aattttacca agttccagag aattttaatt cttctcctaa actatccgag tcagaagcac tttcaaagac tttaaaacat atcggagcta agaagtatat ttgggaatct gttgaacgag aagcagcatt gaaacgtaag aagaatgatc caaatgcaag ttattttccg aagggagaac tattagttta tgaacattca ttaggaaaat ccaatactaa aaaagagttt cgattggctt ataaatttgg tatagcctcg atagatccac caatatcgag atacgtgtat gtggattcta actctgggga aattctatcc agtaaagatg ctagaagata cgagggagtt caatttccaa gtccaaaacc tcctatagaa atagatattg attatagtcg ttgtataata gacaaagaat attgtatcga acagggaacg gctatgaccc gttttagcgg attacaatcg attacaactt ggacggcagg aaaaacgaac cattttgaac tgaaagataa ttcgagaggc ggtggaattt atacttactc ttgggaattt gtaaaagatc ctttggaaga tattcaactt ttgaatattc caatgattga tactaataac tcatggtcgc aatctgaata tcatgacaac tacaatcatg atgcgttatt ggatgcacat tggggaattg aaatgactta tgactatttc aaatccattc ataatcggtt gagttatgat ggtgataata gtagaatatt taataacgtg cactatttta atgcgtttgt agcgaataac gcgtattggg atcctgttac ggaagaaata tattacgttt actgtcctca taaaaatagt atatgcaagg gttttaatct accgattctc ttagacccta aatatgaaga ttttacttct ttggatattg tatctcatga atttgggcat ggtatcaacg gggatcttgc gggttttaat cttgatatgg agccaggtgc tctcgatgaa ggttttagcg atatttggaa tgttggtgta aataactacg taaataaagt tttaggaatg caaaagaata tttggttagt aggagatgaa acggttccgg gaggaggaat gcgttctgtg tcaaatccaa aatctactac agtaatgtcc cctgggccaa atacgtatca tggagatctt tgggattttg aagataacga agcacatacg aatagtcttg ttttgagtca ctggttctat actttatcca agggaaaaca aggatttaac gattatgaat gcacgtataa cgtttctgga attggcattg aaaaagccga aagaattgct tatgttgccc ttctttttct ttcttctacg tccggttata cttctgcaag gacttatgcg attattgcgg ctaaactatt gtatggtttg ttttcttcgg aagtaaaaag tacaattgat gcttgggatg cagtagctgt tccggctgag acgacttctc gcggaggaca aggtatggta aggcctcgac attatatcgc ttctgtaaaa ctttctaatg taacaaacga ttccggaaac gattgtggat acaaggataa ttcatactta cttccaacgg tcttgagagg agtgacgtat aacatggtgt tattgagtca gggttcggca agtaatccgt caaaagttca caaatggaga gtatggatcg attttaatca aaacggtagt tttgaatcgt cggaaatggt agttcaggat actgttaatt cttcgtttgg aggaacatta caaaaatcga ttaagatccc aacgaatgca cttactggat atgcgaagat gagagtttct atgaaggcgg ctcaaagtgg agaagcttat caaggttcga gtgaaagctt tgtagaaggg gaagtggagg attatattgt ttcgattcta gatttcagtt tatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat