LpinA.20379.a

BatB (LIC_20041)

CENTER ID: LpinA.20379.a
ORGANISM: Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpinA.20379.a.B2.GE43493 VWFA domain 92 302
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
UniProt: Q75FZ0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNQEFSANLK NIFFALVIVW IIYSTIRIFL ISKIREWKIF YPGLERSSSF PDVRWVLVRI LLIFAVLVCV FFAGLKTEMK DEKKEESFKG VDILFLVDVS LSMQAIDSSP TRLAKFKEVL LRMLPSLSGN RFGMIVFAGS PFLYCPMTTD VSAFSDYVRG LDVDMVGDRG TDLSQAFTKA EALLRSEKVF RNRILILVTD GEDQNDPQAI SFPASFQVWA AGTESGGPIV YNDENSGLNG FLLKDGTLTS NSNSPGIIHS KMNRTFLKNL ADKNDGNFYS LDSNPPDSTI LQKEIFSLEE NLYSRKKDLK RAEGSGKFLF AAVFLLLLDW ILVESLLFPK RKSVSVI
NT Sequence
atgaatcaag agttttccgc taatttaaaa aacatttttt tcgcccttgt aattgtatgg attatatatt caactattag aatatttttg atttctaaaa taagggaatg gaaaattttt tatcctggtt tagaacgttc ttcttcgttt ccggatgttc gttgggtatt ggtaagaatt ttattaattt ttgcggtttt agtctgtgtt ttttttgcag gtctaaaaac ggaaatgaaa gacgaaaaaa aggaagaatc tttcaaagga gtggatatac tctttttagt ggatgtaagt ctttctatgc aggcgataga tagttctcca actcggttgg ctaaatttaa agaagtgtta cttaggatgt tgccctcttt atctggaaac agatttggaa tgattgtatt tgccggaagt ccgtttttat attgtccgat gactacggat gtgtccgctt tttcggatta tgttcgtgga ttggatgtgg atatggtagg ggatcgcgga accgatttga gtcaggcttt tacaaaggcg gaagcgttgc ttcgttccga aaaagtattt cgaaatcgaa ttttgatttt agtaaccgat ggagaagatc aaaatgatcc acaagcgatt tcctttccgg cgagttttca agtttgggct gccggaacgg aaagcggggg cccgatagtt tataacgatg aaaattctgg tttgaacggt tttcttttga aggatggaac tctaacttca aactctaatt ctcctggaat cattcattct aaaatgaatc gaactttttt aaaaaatcta gcagataaaa acgacggaaa tttttattca ctggattcaa atccacccga ttctacaatt ctacaaaagg aaattttttc tttagaggaa aatttatatt ctagaaaaaa agatctaaaa cgcgcggaag gttctggcaa atttttattt gcggcagttt ttctactatt attggattgg attttagtgg aatctttgtt atttcccaaa cggaaatcgg tttctgtaat atga
Details for LpinA.20379.a.B2.GE43493
HARVESTED ON: 11/19/2019
SEQUENCED ON: 12/3/2019
EXPECTED MW: 24kDa
OBSERVED MW: 24kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMD ILFLVDVSLS MQAIDSSPTR LAKFKEVLLR MLPSLSGNRF GMIVFAGSPF LYCPMTTDVS AFSDYVRGLD VDMVGDRGTD LSQAFTKAEA LLRSEKVFRN RILILVTDGE DQNDPQAISF PASFQVWAAG TESGGPIVYN DENSGLNGFL LKDGTLTSNS NSPGIIHSKM NRTFLKNLAD KNDGNFYSLD SNPPDSTILQ KEIFSLEENL
Validated NT Sequence
atcttactca cttataaatt ttcctctaaa gaaaaaattt ccttttgtag aattgtagaa tcgggtggat ttgaatccag tgaataaaaa tttccgtcgt ttttatctgc tagatttttt aaaaaagttc gattcatttt agaatgaatg attccaggag aattagagtt tgaagttaga gttccatcct tcaaaagaaa accgttcaaa ccagaatttt catcgttata aactatcggg cccccgcttt ccgttccggc agcccaaact tgaaaactcg ccggaaagga aatcgcttgt ggatcatttt gatcttctcc atcggttact aaaatcaaaa ttcgatttcg aaatactttt tcggaacgaa gcaacgcttc cgcctttgta aaagcctgac tcaaatcggt tccgcgatcc cctaccatat ccacatccaa tccacgaaca taatccgaaa aagcggacac atccgtagtc atcggacaat ataaaaacgg acttccggca aatacaatca ttccaaatct gtttccagat aaagagggca acatcctaag taacacttct ttaaatttag ccaaccgagt tggagaacta tctatcgcct gcatagaaag acttacatcc actaaaaaga gtatatccat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHDI LFLVDVSLSM QAIDSSPTRL AKFKEVLLRM LPSLSGNRFG MIVFAGSPFL YCPMTTDVSA FSDYVRGLDV DMVGDRGTDL SQAFTKAEAL LRSEKVFRNR ILILVTDGED QNDPQAISFP ASFQVWAAGT ESGGPIVYND ENSGLNGFLL KDGTLTSNSN SPGIIHSKMN RTFLKNLADK NDGNFYSLDS NPPDSTILQK EIFSLEENL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgatatac tctttttagt ggatgtaagt ctttctatgc aggcgataga tagttctcca actcggttgg ctaaatttaa agaagtgtta cttaggatgt tgccctcttt atctggaaac agatttggaa tgattgtatt tgccggaagt ccgtttttat attgtccgat gactacggat gtgtccgctt tttcggatta tgttcgtgga ttggatgtgg atatggtagg ggatcgcgga accgatttga gtcaggcttt tacaaaggcg gaagcgttgc ttcgttccga aaaagtattt cgaaatcgaa ttttgatttt agtaaccgat ggagaagatc aaaatgatcc acaagcgatt tcctttccgg cgagttttca agtttgggct gccggaacgg aaagcggggg cccgatagtt tataacgatg aaaattctgg tttgaacggt tttcttttga aggatggaac tctaacttca aactctaatt ctcctggaat cattcattct aaaatgaatc gaactttttt aaaaaatcta gcagataaaa acgacggaaa tttttattca ctggattcaa atccacccga ttctacaatt ctacaaaagg aaattttttc tttagaggaa aatttatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t