LpinA.19889.a

Putative lipoprotein

CENTER ID: LpinA.19889.a
ORGANISM: Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpinA.19889.a.B2.GE41985 mature protein 20 359
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|267671.5.peg.425
UniProt: Q72VD3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKHFLNIFFV GLLLVGCASE SGENSNASQL LLGIVNHKQL NLAHPERNSE LRILDIPTHY VDSISGNNSN AGTKSAPYRT ITKAIIASKA DGTKVILVAP GTYDKSLGET FPIYIPDGVN LYGDYDGKGL VGGSSSLYAG PPGTTPKTGP TWINGGGDDI PHVNATIIPN NNSQIAGFKI TNPNPDIPDD YSVRGISIQN FVSIKIKNNT ITGMPLGIGI QYEYYHVTAV GHNIISGNQL TFNYHGIINN GIRASYDKVE NNVISKNYIG IGSPIGLDLG QGPTGSAGNN TISCNSYNDI WIPGNGNETQ IIYAMNNNWD HFPPQISFDH SKAGLDIGHV SNATLVYYED GGLAPNACK
NT Sequence
atgaaacatt ttttaaatat ttttttcgta ggattgttat tagtcggctg tgcttcggaa agcggcgaaa acagtaacgc ttcccaattg cttttaggaa ttgtaaatca caaacagctt aaccttgccc atcccgaacg gaattcggaa ctgaggattt tagatatacc gacgcattac gtggattcca tctccggaaa caactccaat gcaggtacta agtccgcccc ctaccgtaca atcacaaagg cgattatagc atccaaggcc gacggtacaa aagtgattct agttgcgccc ggaacctacg acaagtccct aggagagaca tttcctattt atattccgga tggagttaac ttatacggag actatgatgg aaagggtttg gtcggcggtt cttcctccct ttacgcggga ccaccgggaa ccacacctaa aacaggaccc acttggatca acgggggagg agacgacatt ccccacgtga atgccaccat catcccgaac aataactctc aaattgccgg atttaaaatc accaacccga acccggatat tccagatgat tattcagtta gagggatttc tatacagaat ttcgtatcca taaagattaa aaataatacg atcactggaa tgcccttggg aatcggtatc cagtacgaat attatcacgt aacggctgtc ggtcataata taatctctgg aaatcaactc acttttaatt atcatgggat catcaataat ggaattcgag cttcctatga caaagtggag aacaatgtaa tttctaaaaa ttacataggt atcggcagtc caattggact tgatttagga caggggccga ccggaagcgc gggaaataat actatatcct gcaattcata caatgatata tggattccag gaaatggtaa cgaaacacaa attatatacg caatgaataa caattgggat cactttccgc ctcagatttc tttcgaccac agcaaggctg gcttagacat aggacacgtt agtaatgcaa ctcttgttta ctacgaggat gggggattgg ctccaaacgc ttgtaaataa
Details for LpinA.19889.a.B2.GE41985
HARVESTED ON: 10/3/2017
SEQUENCED ON: 10/12/2017
EXPECTED MW: 37kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHME SGENSNASQL LLGIVNHKQL NLAHPERNSE LRILDIPTHY VDSISGNNSN AGTKSAPYRT ITKAIIASKA DGTKVILVAP GTYDKSLGET FPIYIPDGVN LYGDYDGKGL VGGSSSLYAG PPGTTPKTGP TWINGGGDDI PHVNATIIPN NNSQIAGFKI TNPNPDIPDD YSVRGISIQN FVSIKIKNNT ITGMPLGIGI QYEYYHVTAV GHNIISGNQL TFNYHGIINN GIRASYDKVE NNVISKNYIG IGSPIGLDLG QGPTGSAGNN TISCNSYNDI WIPGNGNETQ IIYAMNNNWD HFPPQISFDH SKAGLDIGHV SNATLVYYED G
Validated NT Sequence
cccccatcct cgtagtaaac aagagttgca ttactaacgt gtcctatgtc taagccagcc ttgctgtggt cgaaagaaat ctgaggcgga aagtgatccc aattgttatt cattgcgtat ataatttgtg tttcgttacc atttcctgga atccatatat cattgtatga attgcaggat atagtattat ttcccgcgct tccggtcggc ccctgtccta aatcaagtcc aattggactg ccgataccta tgtaattttt agaaattaca ttgttctcca ctttgtcata ggaagctcga attccattat tgatgatccc atgataatta aaagtgagtt gatttccaga gattatatta tgaccgacag ccgttacgtg ataatattcg tactggatac cgattcccaa gggcattcca gtgatcgtat tatttttaat ctttatggat acgaaattct gtatagaaat ccctctaact gaataatcat ctggaatatc cgggttcggg ttggtgattt taaatccggc aatttgagag ttattgttcg ggatgatggt ggcattcacg tggggaatgt cgtctcctcc cccgttgatc caagtgggtc ctgttttagg tgtggttccc ggtggtcccg cgtaaaggga ggaagaaccg ccgaccaaac cctttccatc atagtctccg tataagttaa ctccatccgg aatataaata ggaaatgtct ctcctaggga cttgtcgtag gttccgggcg caactagaat cacttttgta ccgtcggcct tggatgctat aatcgccttt gtgattgtac ggtagggggc ggacttagta cctgcattgg agttgtttcc ggagatggaa tccacgtaat gcgtcggtat atctaaaatc ctcagttccg aattccgttc gggatgggca aggttaagct gtttgtgatt tacaattcct aaaagcaatt gggaagcgtt actgttttcg ccgctttcca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHES GENSNASQLL LGIVNHKQLN LAHPERNSEL RILDIPTHYV DSISGNNSNA GTKSAPYRTI TKAIIASKAD GTKVILVAPG TYDKSLGETF PIYIPDGVNL YGDYDGKGLV GGSSSLYAGP PGTTPKTGPT WINGGGDDIP HVNATIIPNN NSQIAGFKIT NPNPDIPDDY SVRGISIQNF VSIKIKNNTI TGMPLGIGIQ YEYYHVTAVG HNIISGNQLT FNYHGIINNG IRASYDKVEN NVISKNYIGI GSPIGLDLGQ GPTGSAGNNT ISCNSYNDIW IPGNGNETQI IYAMNNNWDH FPPQISFDHS KAGLDIGHVS NATLVYYEDG GLAPNACK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgaaagcg gcgaaaacag taacgcttcc caattgcttt taggaattgt aaatcacaaa cagcttaacc ttgcccatcc cgaacggaat tcggaactga ggattttaga tataccgacg cattacgtgg attccatctc cggaaacaac tccaatgcag gtactaagtc cgccccctac cgtacaatca caaaggcgat tatagcatcc aaggccgacg gtacaaaagt gattctagtt gcgcccggaa cctacgacaa gtccctagga gagacatttc ctatttatat tccggatgga gttaacttat acggagacta tgatggaaag ggtttggtcg gcggttcttc ctccctttac gcgggaccac cgggaaccac acctaaaaca ggacccactt ggatcaacgg gggaggagac gacattcccc acgtgaatgc caccatcatc ccgaacaata actctcaaat tgccggattt aaaatcacca acccgaaccc ggatattcca gatgattatt cagttagagg gatttctata cagaatttcg tatccataaa gattaaaaat aatacgatca ctggaatgcc cttgggaatc ggtatccagt acgaatatta tcacgtaacg gctgtcggtc ataatataat ctctggaaat caactcactt ttaattatca tgggatcatc aataatggaa ttcgagcttc ctatgacaaa gtggagaaca atgtaatttc taaaaattac ataggtatcg gcagtccaat tggacttgat ttaggacagg ggccgaccgg aagcgcggga aataatacta tatcctgcaa ttcatacaat gatatatgga ttccaggaaa tggtaacgaa acacaaatta tatacgcaat gaataacaat tgggatcact ttccgcctca gatttctttc gaccacagca aggctggctt agacatagga cacgttagta atgcaactct tgtttactac gaggatgggg gattggctcc aaacgcttgt aaatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat