SSGCID: Target Page

LpinA.19877.a

Putative lipoprotein

CENTER ID:	LpinA.19877.a
ORGANISM:	Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LpinA.19877.a.B2.GE41982	soluble domain		31	222

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|267671.5.peg.862
UniProt:	Q72UF3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNQIRSTYFK ILEYSIIVFI GVLGCIKPSS GKFPPLFLSS VTPYTPADLG IETPVADSPL ATLPAISDKE RESIEEAFRL MNENGQLDQK FVKESSIASR DLLFNRPLSD AELEAKVEAE LKGQSYPTPT YGTEQQILLQ ESQAADVFYD RVETDLPNMT VPQLIKVREN FTLSLVMIRF MIDYGNTPNG IPASFLIMAR EKAVAIRQKV NLELIKRGVK SL

NT Sequence

atgaatcaga ttcgttctac atattttaaa attttagaat attcgattat tgtttttata ggagtcctgg gttgtattaa gcctagttcc ggtaaatttc cgccattatt cttatcttcc gtgactccat atactcctgc cgatttgggt atcgaaaccc cggtggcgga ttctccttta gcaacgttac ccgctatttc agacaaggaa cgagaatcta ttgaagaagc gtttcgttta atgaacgaaa atggtcaact agatcagaaa tttgtaaagg aatcttctat agcttccaga gacttacttt ttaatcgccc gttatccgat gccgaattag aagcaaaggt agaagcggaa ttgaaaggtc aatcttatcc gactcctaca tatggtactg aacaacaaat tcttcttcaa gaaagtcagg ctgcagacgt tttttacgat agggtggaaa ctgatcttcc taatatgacg gtccctcagc tgatcaaagt ccgagaaaac tttaccttaa gcttggttat gattcggttt atgatcgatt atggaaatac tcctaacgga attcctgctt cttttttaat tatggcccgc gagaaggcgg tggcgatccg ccaaaaagta aacttagaac tcataaaacg tggtgttaaa tccttatga

Details for LpinA.19877.a.B2.GE41982

HARVESTED ON:	10/3/2017
SEQUENCED ON:	10/12/2017
EXPECTED MW:	22kDa
OBSERVED MW:	23kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG KFPPLFLSSV TPYTPADLGI ETPVADSPLA TLPAISDKER ESIEEAFRLM NENGQLDQKF VKESSIASRD LLFNRPLSDA ELEAKVEAEL KGQSYPTPTY GTEQQILLQE SQAADVFYDR VETDLPNMTV PQLIKVRENF TLSLVMIRFM IDYGNTPNGI PASFLIMARE KAVAIRQKVN LELIKRGVKS L

Validated NT Sequence

atcttactca cttataagga tttaacacca cgttttatga gttctaagtt tactttttgg cggatcgcca ccgccttctc gcgggccata attaaaaaag aagcaggaat tccgttagga gtatttccat aatcgatcat aaaccgaatc ataaccaagc ttaaggtaaa gttttctcgg actttgatca gctgagggac cgtcatatta ggaagatcag tttccaccct atcgtaaaaa acgtctgcag cctgactttc ttgaagaaga atttgttgtt cagtaccata tgtaggagtc ggataagatt gacctttcaa ttccgcttct acctttgctt ctaattcggc atcggataac gggcgattaa aaagtaagtc tctggaagct atagaagatt cctttacaaa tttctgatct agttgaccat tttcgttcat taaacgaaac gcttcttcaa tagattctcg ttccttgtct gaaatagcgg gtaacgttgc taaaggagaa tccgccaccg gggtttcgat acccaaatcg gcaggagtat atggagtcac ggaagataag aataatggcg gaaatttacc catatggtgg tggtggtggt gagccat

Expected Protein Sequence

MAHHHHHHGK FPPLFLSSVT PYTPADLGIE TPVADSPLAT LPAISDKERE SIEEAFRLMN ENGQLDQKFV KESSIASRDL LFNRPLSDAE LEAKVEAELK GQSYPTPTYG TEQQILLQES QAADVFYDRV ETDLPNMTVP QLIKVRENFT LSLVMIRFMI DYGNTPNGIP ASFLIMAREK AVAIRQKVNL ELIKRGVKSL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggtaaat ttccgccatt attcttatct tccgtgactc catatactcc tgccgatttg ggtatcgaaa ccccggtggc ggattctcct ttagcaacgt tacccgctat ttcagacaag gaacgagaat ctattgaaga agcgtttcgt ttaatgaacg aaaatggtca actagatcag aaatttgtaa aggaatcttc tatagcttcc agagacttac tttttaatcg cccgttatcc gatgccgaat tagaagcaaa ggtagaagcg gaattgaaag gtcaatctta tccgactcct acatatggta ctgaacaaca aattcttctt caagaaagtc aggctgcaga cgttttttac gatagggtgg aaactgatct tcctaatatg acggtccctc agctgatcaa agtccgagaa aactttacct taagcttggt tatgattcgg tttatgatcg attatggaaa tactcctaac ggaattcctg cttctttttt aattatggcc cgcgagaagg cggtggcgat ccgccaaaaa gtaaacttag aactcataaa acgtggtgtt aaatccttat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat