LpinA.19852.a

Carboxypeptidase T

CENTER ID: LpinA.19852.a
ORGANISM: Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpinA.19852.a.B2.GE41978 soluble domain 28 506
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|267671.5.peg.1217
UniProt: Q72TN4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKSYLPSNTI FIVYICLFLF VSCAGFFRKK IPNSPLNDPR KMILVRIDPG RLGEFKEKTG GKYNISYQES DSYYSVINKQ DIDKFGFPSP GISVVKQYLP FKYYSGNYQE LINERFTSLE DLKKGYKDNI LNIHYLLGIA NLYSEQSRME VIGKTARGRE IPALLLTNTT TPDEEKISVL FNCAHHANEV ISIEHCYDII YSVLSRPKEY QEILNKMKIW IVPIVNPDGA RHFWHVSNLM GRKNGYPGSG PVNDKLNPGV DINRNYPFYW GKAGGGYSSS NPSNYFYRGP SPGSESETKA MMDLANRERF AASISYHAYA NCLLIPYSID SLNNPEPDVA KEIGKKIAAS VTSLNPEKEF EAKKNIYPID GVDQDYFYFA HGTLAYLLET THLNPEYKEV EKVNVSLRKA WNLLLNEVLE GKKIFLKITD EFGTPLEAKV EIERIKYFQE EIRVSNPING FFFQLFPDRK ETKVKISKEG YEPTEIQTRP NGKWEPLKIV LKKNRI
NT Sequence
atgaaatcgt atcttccttc aaacaccata tttatagtct atatttgtct gtttttattt gtttcctgtg ctggtttctt tcgtaaaaag attccaaatt ctcccttaaa cgatccaaga aaaatgatac tcgttcggat cgatccagga cgactgggag aatttaaaga aaaaaccgga ggaaagtata acatcagtta tcaagaatcg gattcttatt attccgtaat caataaacaa gacatagata agttcggatt tccatctccc ggaatttccg tcgttaaaca atacttacca tttaaatatt attccggcaa ttatcaggaa ttgatcaacg aaagatttac aagtcttgaa gatctaaaaa aaggttataa ggataacata ttaaatattc attatttact tggaatcgca aatttatatt cggaacaatc cagaatggaa gtgatcggca aaaccgctag aggaagagaa attccggccc tacttctaac aaacacaacc actccagacg aggaaaaaat ttccgtactt tttaactgcg ctcaccacgc aaacgaagtt atatccattg aacactgtta cgatataatc tattccgtat tatctagacc aaaagaatat caggaaatcc taaataagat gaaaatctgg attgtaccta ttgtcaatcc agatggcgcc agacattttt ggcacgtatc taacctgatg ggaagaaaaa acggttatcc aggttctggt ccagtaaacg acaagttaaa tccaggagta gatatcaatc gcaattatcc gttttattgg ggaaaagccg gaggaggata ttcttcttct aatccttcta actattttta tagaggcccc tcgccagggt cagaatcaga aacaaaagca atgatggacc ttgcaaatcg ggaaaggttt gcggcatcca ttagctatca tgcatatgca aattgtctgt taattccata ttcgatcgat tcattaaata atccggaacc agacgtggcc aaggaaatag gaaaaaaaat cgctgcttct gtgactagct taaatccaga aaaagaattc gaagcaaaaa aaaatatcta tccaatagac ggagtcgatc aagactactt ctactttgca catggaactc tcgcttatct attggaaacc acacatctca acccggaata caaggaagtg gaaaaagtaa acgtatcctt gaggaaggct tggaacttat tgttaaacga agttttagaa ggaaaaaaga tttttctaaa aatcacggat gaattcggaa caccactcga agcaaaagta gaaatcgaaa gaataaaata ctttcaggaa gaaattcgag tttctaatcc gatcaacggt tttttctttc aactgtttcc agatagaaag gaaactaaag ttaaaatttc gaaagaaggt tacgaaccga cagaaataca aaccagacca aacggcaaat gggaacctct aaaaatcgta cttaagaaaa atagaatata a
Details for LpinA.19852.a.B2.GE41978
HARVESTED ON: 10/3/2017
SEQUENCED ON: 10/12/2017
EXPECTED MW: 56kDa
OBSERVED MW: 56kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMR KKIPNSPLND PRKMILVRID PGRLGEFKEK TGGKYNISYQ ESDSYYSVIN KQDIDKFGFP SPGISVVKQY LPFKYYSGNY QELINERFTS LEDLKKGYKD NILNIHYLLG IANLYSEQSR MEVIGKTARG REIPALLLTN TTTPDEEKIS VLFNCAHHAN EVISIEHCYD IIYSVLSRPK EYQEILNKMK IWIVPIVNPD GARHFWHVSN LMGRKNGYPG SGPVNDKLNP GVDINRNYPF YWGKAGGGYS SSNPSNYFYR GPSPGSESET KAMMDLANRE RFAASISYHA YANCLLIPYS IDSLNNPEPD VAKEIGKKIA ASVTSLNPEK EFEAKKNIYP IDGVDQDYFY FAHGTLAYLL ETTHLNPEYK EVEKVNVSLR KAWNLLLNEV LEGKKIFLKI TDEFGTPLEA KVEIERIKYF QEEIRVSNPI NGFFFQLFPD RKETKVKISK EGYEPTEIQT RPNGKWEPLK
Validated NT Sequence
tttttagagg ttcccatttg ccgtttggtc tggtttgtat ttctgtcggt tcgtaacctt ctttcgaaat tttaacttta gtttcctttc tatctggaaa cagttgaaag aaaaaaccgt tgatcggatt agaaactcga atttcttcct gaaagtattt tattctttcg atttctactt ttgcttcgag tggtgttccg aattcatccg tgatttttag aaaaatcttt tttccttcta aaacttcgtt taacaataag ttccaagcct tcctcaagga tacgtttact ttttccactt ccttgtattc cgggttgaga tgtgtggttt ccaatagata agcgagagtt ccatgtgcaa agtagaagta gtcttgatcg actccgtcta ttggatagat attttttttt gcttcgaatt ctttttctgg atttaagcta gtcacagaag cagcgatttt ttttcctatt tccttggcca cgtctggttc cggattattt aatgaatcga tcgaatatgg aattaacaga caatttgcat atgcatgata gctaatggat gccgcaaacc tttcccgatt tgcaaggtcc atcattgctt ttgtttctga ttctgaccct ggcgaggggc ctctataaaa atagttagaa ggattagaag aagaatatcc tcctccggct tttccccaat aaaacggata attgcgattg atatctactc ctggatttaa cttgtcgttt actggaccag aacctggata accgtttttt cttcccatca ggttagatac gtgccaaaaa tgtctggcgc catctggatt gacaataggt acaatccaga ttttcatctt atttaggatt tcctgatatt cttttggtct agataatacg gaatagatta tatcgtaaca gtgttcaatg gatataactt cgtttgcgtg gtgagcgcag ttaaaaagta cggaaatttt ttcctcgtct ggagtggttg tgtttgttag aagtagggcc ggaatttctc ttcctctagc ggttttgccg atcacttcca ttctggattg ttccgaatat aaatttgcga ttccaagtaa ataatgaata tttaatatgt tatccttata accttttttt agatcttcaa gacttgtaaa tctttcgttg atcaattcct gataattgcc ggaataatat ttaaatggta agtattgttt aacgacggaa attccgggag atggaaatcc gaacttatct atgtcttgtt tattgattac ggaataataa gaatccgatt cttgataact gatgttatac tttcctccgg ttttttcttt aaattctccc agtcgtcctg gatcgatccg aacgagtatc atttttcttg gatcgtttaa gggagaattt ggaatctttt tacgcatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHRK KIPNSPLNDP RKMILVRIDP GRLGEFKEKT GGKYNISYQE SDSYYSVINK QDIDKFGFPS PGISVVKQYL PFKYYSGNYQ ELINERFTSL EDLKKGYKDN ILNIHYLLGI ANLYSEQSRM EVIGKTARGR EIPALLLTNT TTPDEEKISV LFNCAHHANE VISIEHCYDI IYSVLSRPKE YQEILNKMKI WIVPIVNPDG ARHFWHVSNL MGRKNGYPGS GPVNDKLNPG VDINRNYPFY WGKAGGGYSS SNPSNYFYRG PSPGSESETK AMMDLANRER FAASISYHAY ANCLLIPYSI DSLNNPEPDV AKEIGKKIAA SVTSLNPEKE FEAKKNIYPI DGVDQDYFYF AHGTLAYLLE TTHLNPEYKE VEKVNVSLRK AWNLLLNEVL EGKKIFLKIT DEFGTPLEAK VEIERIKYFQ EEIRVSNPIN GFFFQLFPDR KETKVKISKE GYEPTEIQTR PNGKWEPLKI VLKKNRI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcgtaaaa agattccaaa ttctccctta aacgatccaa gaaaaatgat actcgttcgg atcgatccag gacgactggg agaatttaaa gaaaaaaccg gaggaaagta taacatcagt tatcaagaat cggattctta ttattccgta atcaataaac aagacataga taagttcgga tttccatctc ccggaatttc cgtcgttaaa caatacttac catttaaata ttattccggc aattatcagg aattgatcaa cgaaagattt acaagtcttg aagatctaaa aaaaggttat aaggataaca tattaaatat tcattattta cttggaatcg caaatttata ttcggaacaa tccagaatgg aagtgatcgg caaaaccgct agaggaagag aaattccggc cctacttcta acaaacacaa ccactccaga cgaggaaaaa atttccgtac tttttaactg cgctcaccac gcaaacgaag ttatatccat tgaacactgt tacgatataa tctattccgt attatctaga ccaaaagaat atcaggaaat cctaaataag atgaaaatct ggattgtacc tattgtcaat ccagatggcg ccagacattt ttggcacgta tctaacctga tgggaagaaa aaacggttat ccaggttctg gtccagtaaa cgacaagtta aatccaggag tagatatcaa tcgcaattat ccgttttatt ggggaaaagc cggaggagga tattcttctt ctaatccttc taactatttt tatagaggcc cctcgccagg gtcagaatca gaaacaaaag caatgatgga ccttgcaaat cgggaaaggt ttgcggcatc cattagctat catgcatatg caaattgtct gttaattcca tattcgatcg attcattaaa taatccggaa ccagacgtgg ccaaggaaat aggaaaaaaa atcgctgctt ctgtgactag cttaaatcca gaaaaagaat tcgaagcaaa aaaaaatatc tatccaatag acggagtcga tcaagactac ttctactttg cacatggaac tctcgcttat ctattggaaa ccacacatct caacccggaa tacaaggaag tggaaaaagt aaacgtatcc ttgaggaagg cttggaactt attgttaaac gaagttttag aaggaaaaaa gatttttcta aaaatcacgg atgaattcgg aacaccactc gaagcaaaag tagaaatcga aagaataaaa tactttcagg aagaaattcg agtttctaat ccgatcaacg gttttttctt tcaactgttt ccagatagaa aggaaactaa agttaaaatt tcgaaagaag gttacgaacc gacagaaata caaaccagac caaacggcaa atgggaacct ctaaaaatcg tacttaagaa aaatagaata tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat