LpinA.19850.a

ABC transporter periplasmic dipeptide-binding protein

CENTER ID: LpinA.19850.a
ORGANISM: Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpinA.19850.a.B2.GE41977 mature protein 30 519
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|267671.5.peg.631
UniProt: Q72UX4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDGFSLLNTY LTFFAGILVL SLLFSGCKSR EEDPEELILS IPSDPISLDP LFSTDLSSRT LGKFLYPFLF QKVQNDKSNY QLVESFQLVG TGKIRSLKLK LRTYTLSNGE KLKAEQVLES LNRLVNTPGP RKSQYSFIKE INVLSEKEIE LKITGGLRHA VDLLSLPPAG IVCCKTADSK KLEPESFETL EKKNVLEIPK AGRYFLREWK KNNYILLEKN PYVNEDLPKK IRLRILASAS TGVFLFSKGR MDLMRIPNFL LKNPHIQKEN LRFRKGGGIQ YIAINANEPC FDLNFRKALN TSVDANLIIQ KLLEGNADLT FGPVPLGTIG LSKIRKIIEE NPEILSIQNH SHKKANEYLK KSSCYPKILE REIDFRMRGD EENNANGLAL VSFLNKLGLK VKIRPMEKTT LYKENSEGKG DLTLLFWYAD LPGVWNFIDP LFSGRDKGNG GNRAHYENPE LENLFSKVRN SDSLSLESEA TQALRILLRE NPWIFLWSPY EAFLISEKAK RYSNLEDYL
NT Sequence
ttggacgggt ttagtctttt gaatacttat ctaacctttt ttgcaggaat cctcgttttg tcccttcttt tttccggttg taaatctaga gaagaggatc ctgaagaatt gattctttct attccatccg atccgattag tttggatccg cttttctcta ccgatttgtc ttctcgcacg ctcggaaaat ttttatatcc gtttttattc caaaaagtac agaacgataa atctaactat caacttgtag aatcgtttca attggtaggt accggaaaaa ttcgttctct caaactcaag cttagaacgt atactttgtc caacggggaa aaattaaaag cagaacaggt tctagaatct ttgaatcggt tagtgaatac tcctggtcct agaaaaagcc aatactcttt tattaaagaa atcaacgttc tttcggaaaa agaaatagaa ctaaaaatta cgggcggact taggcacgca gtggatttac tttctttacc acctgctgga attgtttgtt gcaaaaccgc agattcaaaa aaattggaac ctgaatcgtt tgaaacatta gaaaaaaaga atgtattgga gattcctaag gccggaagat actttctgag agagtggaaa aaaaataatt atatactttt agaaaaaaat ccttatgtaa acgaagatct tccaaaaaaa atccgtttga gaattttggc ttccgcttct acgggtgtgt ttcttttttc caagggaaga atggatctga tgcgtattcc aaatttctta ttaaaaaatc ctcatattca aaaagagaat cttagattta gaaaaggagg aggtattcaa tacatagcta tcaatgcgaa cgaaccttgt tttgatttga attttagaaa agctttaaat acttctgtag acgctaatct aataatccaa aaattattgg aagggaatgc ggatctaact tttggtccgg ttcctttggg aactatcggg ttatctaaaa ttagaaagat tatagaggag aatcccgaaa ttctttcgat tcaaaatcat tctcataaga aagcgaatga atatctaaaa aagtcttctt gttatccgaa aattttagaa agagaaattg attttagaat gagaggagac gaggagaata acgcgaatgg tttggctttg gtcagttttt taaacaaact cggattaaag gttaaaattc gtcctatgga aaaaacgact ctttataaag aaaattcaga aggaaaagga gatctaacgt tacttttttg gtacgcggat ttaccggggg tttggaactt tatagatccc ttattttctg gtagagacaa gggtaatggc ggaaacaggg cgcactatga aaatccggaa cttgaaaatt tattttccaa ggttagaaat tccgattcct tgagtttgga atcggaagcg actcaggctt taaggattct tctcagagaa aatccttgga tctttctttg gtcaccatat gaagcttttc taatatccga aaaagcaaaa cgttattcta atttggagga ttatctgtag
Details for LpinA.19850.a.B2.GE41977
HARVESTED ON: 10/3/2017
SEQUENCED ON: 10/12/2017
EXPECTED MW: 57kDa
OBSERVED MW: 57kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMR EEDPEELILS IPSDPISLDP LFSTDLSSRT LGKFLYPFLF QKVQNDKSNY QLVESFQLVG TGKIRSLKLK LRTYTLSNGE KLKAEQVLES LNRLVNTPGP RKSQYSFIKE INVLSEKEIE LKITGGLRHA VDLLSLPPAG IVCCKTADSK KLEPESFETL EKKNVLEIPK AGRYFLREWK KNNYILLEKN PYVNEDLPKK IRLRILASAS TGVFLFSKGR MDLMRIPNFL LKNPHIQKEN LRFRKGGGIQ YIAINANEPC FDLNFRKALN TSVDANLIIQ KLLEGNADLT FGPVPLGTIG LSKIRKIIEE NPEILSIQNH SHKKANEYLK KSSCYPKILE REIDFRMRGD EENNANGLAL VSFLNKLGLK VKIRPMEKTT LYKENSEGKG DLTLLFWYAD LPGVWNFIDP LFSGRDKGNG GNRAHYENPE LENLFSKVRN SDSLSLESEA TQALRILLRE NPWIFLWSPY EAFLISEKA
Validated NT Sequence
tttgcttttt cggatattag aaaagcttca tatggtgacc aaagaaagat ccaaggattt tctctgagaa gaatccttaa agcctgagtc gcttccgatt ccaaactcaa ggaatcggaa tttctaacct tggaaaataa attttcaagt tccggatttt catagtgcgc cctgtttccg ccattaccct tgtctctacc agaaaataag ggatctataa agttccaaac ccccggtaaa tccgcgtacc aaaaaagtaa cgttagatct ccttttcctt ctgaattttc tttataaaga gtcgtttttt ccataggacg aattttaacc tttaatccga gtttgtttaa aaaactgacc aaagccaaac cattcgcgtt attctcctcg tctcctctca ttctaaaatc aatttctctt tctaaaattt tcggataaca agaagacttt tttagatatt cattcgcttt cttatgagaa tgattttgaa tcgaaagaat ttcgggattc tcctctataa tctttctaat tttagataac ccgatagttc ccaaaggaac cggaccaaaa gttagatccg cattcccttc caataatttt tggattatta gattagcgtc tacagaagta tttaaagctt ttctaaaatt caaatcaaaa caaggttcgt tcgcattgat agctatgtat tgaatacctc ctccttttct aaatctaaga ttctcttttt gaatatgagg attttttaat aagaaatttg gaatacgcat cagatccatt cttcccttgg aaaaaagaaa cacacccgta gaagcggaag ccaaaattct caaacggatt ttttttggaa gatcttcgtt tacataagga tttttttcta aaagtatata attatttttt ttccactctc tcagaaagta tcttccggcc ttaggaatct ccaatacatt ctttttttct aatgtttcaa acgattcagg ttccaatttt tttgaatctg cggttttgca acaaacaatt ccagcaggtg gtaaagaaag taaatccact gcgtgcctaa gtccgcccgt aatttttagt tctatttctt tttccgaaag aacgttgatt tctttaataa aagagtattg gctttttcta ggaccaggag tattcactaa ccgattcaaa gattctagaa cctgttctgc ttttaatttt tccccgttgg acaaagtata cgttctaagc ttgagtttga gagaacgaat ttttccggta cctaccaatt gaaacgattc tacaagttga tagttagatt tatcgttctg tactttttgg aataaaaacg gatataaaaa ttttccgagc gtgcgagaag acaaatcggt agagaaaagc ggatccaaac taatcggatc ggatggaata gaaagaatca attcttcagg atcctcttct ctcatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHRE EDPEELILSI PSDPISLDPL FSTDLSSRTL GKFLYPFLFQ KVQNDKSNYQ LVESFQLVGT GKIRSLKLKL RTYTLSNGEK LKAEQVLESL NRLVNTPGPR KSQYSFIKEI NVLSEKEIEL KITGGLRHAV DLLSLPPAGI VCCKTADSKK LEPESFETLE KKNVLEIPKA GRYFLREWKK NNYILLEKNP YVNEDLPKKI RLRILASAST GVFLFSKGRM DLMRIPNFLL KNPHIQKENL RFRKGGGIQY IAINANEPCF DLNFRKALNT SVDANLIIQK LLEGNADLTF GPVPLGTIGL SKIRKIIEEN PEILSIQNHS HKKANEYLKK SSCYPKILER EIDFRMRGDE ENNANGLALV SFLNKLGLKV KIRPMEKTTL YKENSEGKGD LTLLFWYADL PGVWNFIDPL FSGRDKGNGG NRAHYENPEL ENLFSKVRNS DSLSLESEAT QALRILLREN PWIFLWSPYE AFLISEKAKR YSNLEDYL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catagagaag aggatcctga agaattgatt ctttctattc catccgatcc gattagtttg gatccgcttt tctctaccga tttgtcttct cgcacgctcg gaaaattttt atatccgttt ttattccaaa aagtacagaa cgataaatct aactatcaac ttgtagaatc gtttcaattg gtaggtaccg gaaaaattcg ttctctcaaa ctcaagctta gaacgtatac tttgtccaac ggggaaaaat taaaagcaga acaggttcta gaatctttga atcggttagt gaatactcct ggtcctagaa aaagccaata ctcttttatt aaagaaatca acgttctttc ggaaaaagaa atagaactaa aaattacggg cggacttagg cacgcagtgg atttactttc tttaccacct gctggaattg tttgttgcaa aaccgcagat tcaaaaaaat tggaacctga atcgtttgaa acattagaaa aaaagaatgt attggagatt cctaaggccg gaagatactt tctgagagag tggaaaaaaa ataattatat acttttagaa aaaaatcctt atgtaaacga agatcttcca aaaaaaatcc gtttgagaat tttggcttcc gcttctacgg gtgtgtttct tttttccaag ggaagaatgg atctgatgcg tattccaaat ttcttattaa aaaatcctca tattcaaaaa gagaatctta gatttagaaa aggaggaggt attcaataca tagctatcaa tgcgaacgaa ccttgttttg atttgaattt tagaaaagct ttaaatactt ctgtagacgc taatctaata atccaaaaat tattggaagg gaatgcggat ctaacttttg gtccggttcc tttgggaact atcgggttat ctaaaattag aaagattata gaggagaatc ccgaaattct ttcgattcaa aatcattctc ataagaaagc gaatgaatat ctaaaaaagt cttcttgtta tccgaaaatt ttagaaagag aaattgattt tagaatgaga ggagacgagg agaataacgc gaatggtttg gctttggtca gttttttaaa caaactcgga ttaaaggtta aaattcgtcc tatggaaaaa acgactcttt ataaagaaaa ttcagaagga aaaggagatc taacgttact tttttggtac gcggatttac cgggggtttg gaactttata gatcccttat tttctggtag agacaagggt aatggcggaa acagggcgca ctatgaaaat ccggaacttg aaaatttatt ttccaaggtt agaaattccg attccttgag tttggaatcg gaagcgactc aggctttaag gattcttctc agagaaaatc cttggatctt tctttggtca ccatatgaag cttttctaat atccgaaaaa gcaaaacgtt attctaattt ggaggattat ctgtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat