LpinA.19842.a

Uncharacterized protein

CENTER ID: LpinA.19842.a
ORGANISM: Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpinA.19842.a.B2.GE41974 mature protein 18 358
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|267671.5.peg.1190
UniProt: Q72TP9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFYRILIFVL VLVSCSPASI YNPSIFMSQA WIENTILDCI LKECYLCSLK ITNKPVVSLF AGTGVAASVD GTTSTASFKT PFGLEVDTSG NIYVSDQINN LIRKIDPSGN VKTLSTNLPL QDPSGIKFDP LTGDKYVSCK DSNQIYKIDS TEQFSLFAGS SSDLSGLQNG DRLNSLFDSP FFMDIDPERN LYVGELSNHT IRKINLNSGT VSTLSGGISG YLDGDLASAR FKSPLGIAYN RKMNSLLAAD IQDHRIRKID LKNSTVSTLL GNGIGADVDG NGTNASFFGP AFISIDNSGY MFVSDANSNR IRIVDPLLNV STIDHTFMEI GTVKVDCLNQ RLLVADSRAN QIFQVKFE
NT Sequence
atgttctata gaattttgat tttcgttcta gtattggttt cctgttctcc tgcctctatt tataatccga gtatttttat gagccaggct tggattgaaa acacgatcct ggactgtatt cttaaagaat gttatctttg ttctctcaaa attacaaaca aacctgttgt tagtttgttt gccggtactg gtgtagcagc aagcgtagat ggtaccactt ccacagcctc tttcaaaact cctttcggtt tagaagtaga tacttccgga aatatctatg tgagcgatca aataaacaac ctgattcgta aaattgatcc ctccggaaat gtaaaaactc tttctacaaa cttaccttta caagatcctt ccggcatcaa gttcgatccg cttactggag acaaatacgt ttcctgcaaa gacagtaatc aaatttataa gatagattct acggaacaat tttccttatt tgccggaagt tcttccgact tgagtggtct tcaaaacggg gatagactca attccttgtt tgacagtcct ttttttatgg acatcgatcc agaaagaaac ttatacgtgg gagagttaag caatcatacg attcgaaaaa tcaatctcaa ctcaggtacg gtgagtactc tttccggagg aatttctggt tatttagacg gagatttagc ttcagctcgt tttaaatctc cattaggaat tgcttataat cgtaaaatga atagtttatt ggctgcagat attcaggatc atagaattcg aaaaatagat ctcaaaaatt ctaccgtatc cactcttttg ggaaacggaa tcggagcaga cgtagatgga aatggaacaa acgcttcgtt ttttggtccc gctttcattt ctatagataa cagcggttat atgtttgtat cggacgctaa ttctaacaga atccgcattg tggatccttt acttaacgtt tctacaattg atcatacgtt tatggagata gggacagtta aagttgattg tttgaaccaa agattgttag tcgccgattc tcgtgcaaat cagattttcc aagttaaatt tgaatga
Details for LpinA.19842.a.B2.GE41974
HARVESTED ON: 10/3/2017
SEQUENCED ON: 10/12/2017
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMA SIYNPSIFMS QAWIENTILD CILKECYLCS LKITNKPVVS LFAGTGVAAS VDGTTSTASF KTPFGLEVDT SGNIYVSDQI NNLIRKIDPS GNVKTLSTNL PLQDPSGIKF DPLTGDKYVS CKDSNQIYKI DSTEQFSLFA GSSSDLSGLQ NGDRLNSLFD SPFFMDIDPE RNLYVGELSN HTIRKINLNS GTVSTLSGGI SGYLDGDLAS ARFKSPLGIA YNRKMNSLLA ADIQDHRIRK IDLKNSTVST LLGNGIGADV DGNGTNASFF GPAFISIDNS GYMFVSDANS NRIRIVDPLL NVSTIDHTFM EIGTVKVDCL NQRLLVADSR AN
Validated NT Sequence
tgatttgcac gagaatcggc gactaacaat ctttggttca aacaatcaac tttaactgtc cctatctcca taaacgtatg atcaattgta gaaacgttaa gtaaaggatc cacaatgcgg attctgttag aattagcgtc cgatacaaac atataaccgc tgttatctat agaaatgaaa gcgggaccaa aaaacgaagc gtttgttcca tttccatcta cgtctgctcc gattccgttt cccaaaagag tggatacggt agaatttttg agatctattt ttcgaattct atgatcctga atatctgcag ccaataaact attcatttta cgattataag caattcctaa tggagattta aaacgagctg aagctaaatc tccgtctaaa taaccagaaa ttcctccgga aagagtactc accgtacctg agttgagatt gatttttcga atcgtatgat tgcttaactc tcccacgtat aagtttcttt ctggatcgat gtccataaaa aaaggactgt caaacaagga attgagtcta tccccgtttt gaagaccact caagtcggaa gaacttccgg caaataagga aaattgttcc gtagaatcta tcttataaat ttgattactg tctttgcagg aaacgtattt gtctccagta agcggatcga acttgatgcc ggaaggatct tgtaaaggta agtttgtaga aagagttttt acatttccgg agggatcaat tttacgaatc aggttgttta tttgatcgct cacatagata tttccggaag tatctacttc taaaccgaaa ggagttttga aagaggctgt ggaagtggta ccatctacgc ttgctgctac accagtaccg gcaaacaaac taacaacagg tttgtttgta attttgagag aacaaagata acattcttta agaatacagt ccaggatcgt gttttcaatc caagcctggc tcataaaaat actcggatta taaatagagg ccatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHAS IYNPSIFMSQ AWIENTILDC ILKECYLCSL KITNKPVVSL FAGTGVAASV DGTTSTASFK TPFGLEVDTS GNIYVSDQIN NLIRKIDPSG NVKTLSTNLP LQDPSGIKFD PLTGDKYVSC KDSNQIYKID STEQFSLFAG SSSDLSGLQN GDRLNSLFDS PFFMDIDPER NLYVGELSNH TIRKINLNSG TVSTLSGGIS GYLDGDLASA RFKSPLGIAY NRKMNSLLAA DIQDHRIRKI DLKNSTVSTL LGNGIGADVD GNGTNASFFG PAFISIDNSG YMFVSDANSN RIRIVDPLLN VSTIDHTFME IGTVKVDCLN QRLLVADSRA NQIFQVKFE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcctcta tttataatcc gagtattttt atgagccagg cttggattga aaacacgatc ctggactgta ttcttaaaga atgttatctt tgttctctca aaattacaaa caaacctgtt gttagtttgt ttgccggtac tggtgtagca gcaagcgtag atggtaccac ttccacagcc tctttcaaaa ctcctttcgg tttagaagta gatacttccg gaaatatcta tgtgagcgat caaataaaca acctgattcg taaaattgat ccctccggaa atgtaaaaac tctttctaca aacttacctt tacaagatcc ttccggcatc aagttcgatc cgcttactgg agacaaatac gtttcctgca aagacagtaa tcaaatttat aagatagatt ctacggaaca attttcctta tttgccggaa gttcttccga cttgagtggt cttcaaaacg gggatagact caattccttg tttgacagtc ctttttttat ggacatcgat ccagaaagaa acttatacgt gggagagtta agcaatcata cgattcgaaa aatcaatctc aactcaggta cggtgagtac tctttccgga ggaatttctg gttatttaga cggagattta gcttcagctc gttttaaatc tccattagga attgcttata atcgtaaaat gaatagttta ttggctgcag atattcagga tcatagaatt cgaaaaatag atctcaaaaa ttctaccgta tccactcttt tgggaaacgg aatcggagca gacgtagatg gaaatggaac aaacgcttcg ttttttggtc ccgctttcat ttctatagat aacagcggtt atatgtttgt atcggacgct aattctaaca gaatccgcat tgtggatcct ttacttaacg tttctacaat tgatcatacg tttatggaga tagggacagt taaagttgat tgtttgaacc aaagattgtt agtcgccgat tctcgtgcaa atcagatttt ccaagttaaa tttgaatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t