SSGCID: Target Page

LpinA.19839.a

Putative lipoprotein

CENTER ID:	LpinA.19839.a
ORGANISM:	Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LpinA.19839.a.B2.GE41973	mature protein		29	238

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|267671.5.peg.3722
UniProt:	Q72MW4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MANNVLMFSL RIFLLFFFFL SFSSACYLNP VFKDLVSSDE KEESLIPLLL ILSSTAVSPP VIQGETIFFP STGLTWMRCS RGQTYNASSD SCTGFLVSPQ YCSSLDNQCN GTSGGTLISG SAFDSCSTFQ VSGKSATWRV PTHSELKGII FCSNGTDLTQ SQDYSKTCAS SGTSYEAPTI RKDWFPGNPT GSLIEFWSST SDPQNSAVAW KVNFTNGSTN ITSPKNVSGY LRCVSNSR

NT Sequence

atggcgaata acgttctaat gttttcttta agaatttttc ttttgttttt tttcttttta agttttagct ctgcttgtta tttgaatcct gtttttaagg atttagtttc ttccgatgaa aaagaagaat ctttaattcc tttgttattg atcttatctt cgactgcggt ttctcctcca gtgatccaag gagaaacgat ctttttccca tctaccggtc ttacttggat gagatgttct agaggtcaga cctacaacgc ctcttccgat tcttgtactg gttttttagt ttctcctcag tattgttcta gtttagacaa ccaatgtaat ggaacttcgg gaggtacttt aatttctgga tctgctttcg attcttgttc cactttccag gtttccggta agtctgcaac ttggagagtt cctacacatt ccgaattaaa gggaattata ttttgttcga acggaacgga tctgacccag tcacaggatt attctaaaac ctgtgcttct tctggaacca gttatgaagc tcctacaatt cgaaaggatt ggtttcctgg aaatcctact ggaagtctaa ttgaattttg gagtagcacg agtgatcctc agaattctgc agttgcttgg aaggtaaatt ttacgaacgg ttctacaaac attacttctc caaaaaatgt ttccgggtat cttcgttgtg tgagtaattc tcgttga

Details for LpinA.19839.a.B2.GE41973

HARVESTED ON:	10/3/2017
SEQUENCED ON:	10/12/2017
EXPECTED MW:	24kDa
OBSERVED MW:	24kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMN PVFKDLVSSD EKEESLIPLL LILSSTAVSP PVIQGETIFF PSTGLTWMRC SRGQTYNASS DSCTGFLVSP QYCSSLDNQC NGTSGGTLIS GSAFDSCSTF QVSGKSATWR VPTHSELKGI IFCSNGTDLT QSQDYSKTCA SSGTSYEAPT IRKDWFPGNP TGSLIEFWSS TSDPQNSAVA WKVNFTNGST NITSPKNVSG YLRCVSNSR

Validated NT Sequence

atcttactca cttaacgaga attactcaca caacgaagat acccggaaac attttttgga gaagtaatgt ttgtagaacc gttcgtaaaa tttaccttcc aagcaactgc agaattctga ggatcactcg tgctactcca aaattcaatt agacttccag taggatttcc aggaaaccaa tcctttcgaa ttgtaggagc ttcataactg gttccagaag aagcacaggt tttagaataa tcctgtgact gggtcagatc cgttccgttc gaacaaaata taattccctt taattcggaa tgtgtaggaa ctctccaagt tgcagactta ccggaaacct ggaaagtgga acaagaatcg aaagcagatc cagaaattaa agtacctccc gaagttccat tacattggtt gtctaaacta gaacaatact gaggagaaac taaaaaacca gtacaagaat cggaagaggc gttgtaggtc tgacctctag aacatctcat ccaagtaaga ccggtagatg ggaaaaagat cgtttctcct tggatcactg gaggagaaac cgcagtcgaa gataagatca ataacaaagg aattaaagat tcttcttttt catcggaaga aactaaatcc ttaaaaacag gattcatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

MAHHHHHHNP VFKDLVSSDE KEESLIPLLL ILSSTAVSPP VIQGETIFFP STGLTWMRCS RGQTYNASSD SCTGFLVSPQ YCSSLDNQCN GTSGGTLISG SAFDSCSTFQ VSGKSATWRV PTHSELKGII FCSNGTDLTQ SQDYSKTCAS SGTSYEAPTI RKDWFPGNPT GSLIEFWSST SDPQNSAVAW KVNFTNGSTN ITSPKNVSGY LRCVSNSR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataatcctg tttttaagga tttagtttct tccgatgaaa aagaagaatc tttaattcct ttgttattga tcttatcttc gactgcggtt tctcctccag tgatccaagg agaaacgatc tttttcccat ctaccggtct tacttggatg agatgttcta gaggtcagac ctacaacgcc tcttccgatt cttgtactgg ttttttagtt tctcctcagt attgttctag tttagacaac caatgtaatg gaacttcggg aggtacttta atttctggat ctgctttcga ttcttgttcc actttccagg tttccggtaa gtctgcaact tggagagttc ctacacattc cgaattaaag ggaattatat tttgttcgaa cggaacggat ctgacccagt cacaggatta ttctaaaacc tgtgcttctt ctggaaccag ttatgaagct cctacaattc gaaaggattg gtttcctgga aatcctactg gaagtctaat tgaattttgg agtagcacga gtgatcctca gaattctgca gttgcttgga aggtaaattt tacgaacggt tctacaaaca ttacttctcc aaaaaatgtt tccgggtatc ttcgttgtgt gagtaattct cgttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat