LpinA.19836.a

Putative lipoprotein

CENTER ID: LpinA.19836.a
ORGANISM: Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpinA.19836.a.B2.GE41971 mature protein 21 347
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpinA.19836.a.B2.PS38502 mature protein 21 347

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|267671.5.peg.102
UniProt: Q72W49

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFHFKTILFV LTGFIFFVSA CKTPPPKDPW IVPSLKENEK LFLAPLKVDE FVSKDLGGRH YPASNELRID LFKPYIENLE GGYLGAGTDQ NFTFIVWAKS KYVWLMDFDY TICLINRIHL LFFRIAVDPE SYRELWARKN KHTSFEIIKK EWEKDPEWPL IREAWEVAHR GKSDVPQRWN ELDRTSQRFG LKTFIHSKEE YNYIRNMVLQ GRIQILKGDI NAEKSMRSVA ERAARLNVPI RVVYLSNIED YFSYSDSFRD NLLSLPTDEK GVVLRTMQNG TKEEYGSPDG EKIPVDYPLH YNVQPLENLQ DWMLLSGHLH KGILMQFRTP IQKGFSIIKS GPVEVLK
NT Sequence
atgtttcatt ttaaaacgat cctgtttgtt ttaactggat ttattttttt cgtttcggct tgtaaaacgc ctcctcctaa agatccgtgg atcgttcctt ctcttaaaga gaatgaaaaa ctatttttag ctcctttgaa agttgatgag tttgtttcta aagatttagg aggaaggcat tatcctgcat cgaacgaact cagaatcgat cttttcaaac cgtatattga aaatttagaa ggtggttatt taggtgctgg aacggatcag aactttacat ttatcgtttg ggcaaagagt aaatatgtat ggttaatgga ttttgattat acgatttgtc taattaatcg aattcatctt ttgtttttta gaattgccgt agatcctgaa tcgtatcggg agctttgggc tcgaaaaaat aaacatactt cttttgaaat cataaagaaa gaatgggaga aagatccaga atggccactc attcgagaag cttgggaagt ggctcataga ggcaaatcag acgttccaca aagatggaat gagcttgatc gaacgtcgca aagatttggc cttaagacgt ttattcattc taaagaagaa tacaattaca ttcgaaacat ggttcttcaa ggaagaatac aaatattaaa aggggacatt aatgctgaaa aaagtatgag atctgtggcg gagagagccg caagattgaa cgttccaatt cgagttgttt atctttctaa tatagaagac tatttttctt acagtgatag ttttcgagac aacctactga gtttacccac ggatgaaaag ggagttgttt tgagaacaat gcaaaatgga accaaggaag aatatggatc acctgacgga gaaaaaattc cggtggatta tcctttacat tataatgttc aacctctgga aaatttgcag gattggatgt tattatctgg tcatcttcat aagggaatcc taatgcaatt cagaactcct attcaaaaag gtttttctat aatcaaaagt gggcccgtag aagttttgaa atga
Details for LpinA.19836.a.B2.GE41971
HARVESTED ON: 10/3/2017
SEQUENCED ON: 10/12/2017
EXPECTED MW: 39kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMC KTPPPKDPWI VPSLKENEKL FLAPLKVDEF VSKDLGGRHY PASNELRIDL FKPYIENLEG GYLGAGTDQN FTFIVWAKSK YVWLMDFDYT ICLINRIHLL FFRIAVDPES YRELWARKNK HTSFEIIKKE WEKDPEWPLI REAWEVAHRG KSDVPQRWNE LDRTSQRFGL KTFIHSKEEY NYIRNMVLQG RIQILKGDIN AEKSMRSVAE RAARLNVPIR VVYLSNIEDY FSYSDSFRDN LLSLPTDEKG VVLRTMQNGT KEEYGSPDGE KIPVDYPLHY NVQPLENLQD WMLLSGHLHK GILMQFRTPI QKGFSIIKXX
Validated NT Sequence
gggccnnctt ttgattatag aaaaaccttt ttgaatagga gttctgaatt gcattaggat tcccttatga agatgaccag ataataacat ccaatcctgc aaattttcca gaggttgaac attataatgt aaaggataat ccaccggaat tttttctccg tcaggtgatc catattcttc cttggttcca ttttgcattg ttctcaaaac aactcccttt tcatccgtgg gtaaactcag taggttgtct cgaaaactat cactgtaaga aaaatagtct tctatattag aaagataaac aactcgaatt ggaacgttca atcttgcggc tctctccgcc acagatctca tacttttttc agcattaatg tcccctttta atatttgtat tcttccttga agaaccatgt ttcgaatgta attgtattct tctttagaat gaataaacgt cttaaggcca aatctttgcg acgttcgatc aagctcattc catctttgtg gaacgtctga tttgcctcta tgagccactt cccaagcttc tcgaatgagt ggccattctg gatctttctc ccattctttc tttatgattt caaaagaagt atgtttattt tttcgagccc aaagctcccg atacgattca ggatctacgg caattctaaa aaacaaaaga tgaattcgat taattagaca aatcgtataa tcaaaatcca ttaaccatac atatttactc tttgcccaaa cgataaatgt aaagttctga tccgttccag cacctaaata accaccttct aaattttcaa tatacggttt gaaaagatcg attctgagtt cgttcgatgc aggataatgc cttcctccta aatctttaga aacaaactca tcaactttca aaggagctaa aaatagtttt tcattctctt taagagaagg aacgatccac ggatctttag gaggaggcgt tttacacata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHCK TPPPKDPWIV PSLKENEKLF LAPLKVDEFV SKDLGGRHYP ASNELRIDLF KPYIENLEGG YLGAGTDQNF TFIVWAKSKY VWLMDFDYTI CLINRIHLLF FRIAVDPESY RELWARKNKH TSFEIIKKEW EKDPEWPLIR EAWEVAHRGK SDVPQRWNEL DRTSQRFGLK TFIHSKEEYN YIRNMVLQGR IQILKGDINA EKSMRSVAER AARLNVPIRV VYLSNIEDYF SYSDSFRDNL LSLPTDEKGV VLRTMQNGTK EEYGSPDGEK IPVDYPLHYN VQPLENLQDW MLLSGHLHKG ILMQFRTPIQ KGFSIIKSGP VEVLK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattgtaaaa cgcctcctcc taaagatccg tggatcgttc cttctcttaa agagaatgaa aaactatttt tagctccttt gaaagttgat gagtttgttt ctaaagattt aggaggaagg cattatcctg catcgaacga actcagaatc gatcttttca aaccgtatat tgaaaattta gaaggtggtt atttaggtgc tggaacggat cagaacttta catttatcgt ttgggcaaag agtaaatatg tatggttaat ggattttgat tatacgattt gtctaattaa tcgaattcat cttttgtttt ttagaattgc cgtagatcct gaatcgtatc gggagctttg ggctcgaaaa aataaacata cttcttttga aatcataaag aaagaatggg agaaagatcc agaatggcca ctcattcgag aagcttggga agtggctcat agaggcaaat cagacgttcc acaaagatgg aatgagcttg atcgaacgtc gcaaagattt ggccttaaga cgtttattca ttctaaagaa gaatacaatt acattcgaaa catggttctt caaggaagaa tacaaatatt aaaaggggac attaatgctg aaaaaagtat gagatctgtg gcggagagag ccgcaagatt gaacgttcca attcgagttg tttatctttc taatatagaa gactattttt cttacagtga tagttttcga gacaacctac tgagtttacc cacggatgaa aagggagttg ttttgagaac aatgcaaaat ggaaccaagg aagaatatgg atcacctgac ggagaaaaaa ttccggtgga ttatccttta cattataatg ttcaacctct ggaaaatttg caggattgga tgttattatc tggtcatctt cataagggaa tcctaatgca attcagaact cctattcaaa aaggtttttc tataatcaaa agtgggcccg tagaagtttt gaaatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for LpinA.19836.a.B2.PS38502
PURIFICATION DATe: 9/11/2018
CONCENTRATION: 39.48mg/ml
OBSERVED MW: 37kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 1
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMC KTPPPKDPWI VPSLKENEKL FLAPLKVDEF VSKDLGGRHY PASNELRIDL FKPYIENLEG GYLGAGTDQN FTFIVWAKSK YVWLMDFDYT ICLINRIHLL FFRIAVDPES YRELWARKNK HTSFEIIKKE WEKDPEWPLI REAWEVAHRG KSDVPQRWNE LDRTSQRFGL KTFIHSKEEY NYIRNMVLQG RIQILKGDIN AEKSMRSVAE RAARLNVPIR VVYLSNIEDY FSYSDSFRDN LLSLPTDEKG VVLRTMQNGT KEEYGSPDGE KIPVDYPLHY NVQPLENLQD WMLLSGHLHK GILMQFRTPI QKGFSIIKXX
Validated NT Sequence
gggccnnctt ttgattatag aaaaaccttt ttgaatagga gttctgaatt gcattaggat tcccttatga agatgaccag ataataacat ccaatcctgc aaattttcca gaggttgaac attataatgt aaaggataat ccaccggaat tttttctccg tcaggtgatc catattcttc cttggttcca ttttgcattg ttctcaaaac aactcccttt tcatccgtgg gtaaactcag taggttgtct cgaaaactat cactgtaaga aaaatagtct tctatattag aaagataaac aactcgaatt ggaacgttca atcttgcggc tctctccgcc acagatctca tacttttttc agcattaatg tcccctttta atatttgtat tcttccttga agaaccatgt ttcgaatgta attgtattct tctttagaat gaataaacgt cttaaggcca aatctttgcg acgttcgatc aagctcattc catctttgtg gaacgtctga tttgcctcta tgagccactt cccaagcttc tcgaatgagt ggccattctg gatctttctc ccattctttc tttatgattt caaaagaagt atgtttattt tttcgagccc aaagctcccg atacgattca ggatctacgg caattctaaa aaacaaaaga tgaattcgat taattagaca aatcgtataa tcaaaatcca ttaaccatac atatttactc tttgcccaaa cgataaatgt aaagttctga tccgttccag cacctaaata accaccttct aaattttcaa tatacggttt gaaaagatcg attctgagtt cgttcgatgc aggataatgc cttcctccta aatctttaga aacaaactca tcaactttca aaggagctaa aaatagtttt tcattctctt taagagaagg aacgatccac ggatctttag gaggaggcgt tttacacata tggtggtggt ggtggtgagc cat
Expressed Protein Sequence
MAHHHHHHCK TPPPKDPWIV PSLKENEKLF LAPLKVDEFV SKDLGGRHYP ASNELRIDLF KPYIENLEGG YLGAGTDQNF TFIVWAKSKY VWLMDFDYTI CLINRIHLLF FRIAVDPESY RELWARKNKH TSFEIIKKEW EKDPEWPLIR EAWEVAHRGK SDVPQRWNEL DRTSQRFGLK TFIHSKEEYN YIRNMVLQGR IQILKGDINA EKSMRSVAER AARLNVPIRV VYLSNIEDYF SYSDSFRDNL LSLPTDEKGV VLRTMQNGTK EEYGSPDGEK IPVDYPLHYN VQPLENLQDW MLLSGHLHKG ILMQFRTPIQ KGFSIIKSGP VEVLK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTGTAAAA CGCCTCCTCC TAAAGATCCG TGGATCGTTC CTTCTCTTAA AGAGAATGAA AAACTATTTT TAGCTCCTTT GAAAGTTGAT GAGTTTGTTT CTAAAGATTT AGGAGGAAGG CATTATCCTG CATCGAACGA ACTCAGAATC GATCTTTTCA AACCGTATAT TGAAAATTTA GAAGGTGGTT ATTTAGGTGC TGGAACGGAT CAGAACTTTA CATTTATCGT TTGGGCAAAG AGTAAATATG TATGGTTAAT GGATTTTGAT TATACGATTT GTCTAATTAA TCGAATTCAT CTTTTGTTTT TTAGAATTGC CGTAGATCCT GAATCGTATC GGGAGCTTTG GGCTCGAAAA AATAAACATA CTTCTTTTGA AATCATAAAG AAAGAATGGG AGAAAGATCC AGAATGGCCA CTCATTCGAG AAGCTTGGGA AGTGGCTCAT AGAGGCAAAT CAGACGTTCC ACAAAGATGG AATGAGCTTG ATCGAACGTC GCAAAGATTT GGCCTTAAGA CGTTTATTCA TTCTAAAGAA GAATACAATT ACATTCGAAA CATGGTTCTT CAAGGAAGAA TACAAATATT AAAAGGGGAC ATTAATGCTG AAAAAAGTAT GAGATCTGTG GCGGAGAGAG CCGCAAGATT GAACGTTCCA ATTCGAGTTG TTTATCTTTC TAATATAGAA GACTATTTTT CTTACAGTGA TAGTTTTCGA GACAACCTAC TGAGTTTACC CACGGATGAA AAGGGAGTTG TTTTGAGAAC AATGCAAAAT GGAACCAAGG AAGAATATGG ATCACCTGAC GGAGAAAAAA TTCCGGTGGA TTATCCTTTA CATTATAATG TTCAACCTCT GGAAAATTTG CAGGATTGGA TGTTATTATC TGGTCATCTT CATAAGGGAA TCCTAATGCA ATTCAGAACT CCTATTCAAA AAGGTTTTTC TATAATCAAA AGTGGGCCCG TAGAAGTTTT GAAATGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT