LpinA.19822.a

Alpha/beta hydrolase superfamily

CENTER ID: LpinA.19822.a
ORGANISM: Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpinA.19822.a.B2.GE42083 mature protein 18 287
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|267671.5.peg.3116
UniProt: Q72P77

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MYSKFVFFLF LFLGSYCSIP EDFKKKPKES LILLKNSGIN FKEFNFTVEG KQVYGVASGC NLKNQNILIF IHGSPGGWQN YYWYLGNKTL NKKFCIFAMD RPGFGNSNPN EVIPNVEKQA FILGKTIQFF LNQFPLDKNT KIILVGHSYG APIAARISIV FSYKIRTLVL LAAPLSSKEE EIRWYNQIAD WTWVKNLLPL SLKNSNDEMF PLKSQLESLE KFWKLIPCKI ILIHGKKDSL VSFHNLEFFK SIFSPSKLTT IELEKEDHFI PWTQKVFLEK IFLESIE
NT Sequence
atgtattcta aatttgtttt tttcctattt ctttttttgg gaagttattg tagcattcca gaggatttta aaaaaaagcc aaaagaaagt ttaatccttc tcaaaaattc tggaatcaat ttcaaagagt ttaattttac ggtggaagga aaacaagtct atggggttgc atcagggtgt aatttaaaaa atcaaaatat tttaatattt atacatggtt ctcccggagg ttggcaaaat tattattggt atttgggaaa taaaacttta aacaaaaaat tttgtatttt tgccatggac agacccggtt tcggaaattc aaacccaaac gaagtgattc caaacgtgga aaaacaagcc tttatcttag gaaaaacaat tcaatttttt ttaaatcagt tcccattaga caaaaatact aaaatcattc ttgtaggaca ttcttacgga gccccaatcg ctgcaagaat ctctatagtt ttttcttaca agattagaac actcgtttta cttgctgcac ctctaagttc caaagaagaa gaaattcgtt ggtataacca aattgcagat tggacttggg ttaaaaatct tttaccactc agtctcaaaa atagtaatga cgaaatgttt cctcttaaat ctcaattaga atctttagaa aaattttgga aattgattcc ctgtaaaatt attttaatcc acggaaaaaa agattcttta gtatcttttc ataatttaga attttttaaa tctatatttt caccttctaa actcactact atagaactgg aaaaagaaga tcattttatt ccttggactc aaaaagtttt tttagaaaag atttttttag agtcaattga ataa
Details for LpinA.19822.a.B2.GE42083
HARVESTED ON: 12/5/2017
SEQUENCED ON: 12/7/2017
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS IPEDFKKKPK ESLILLKNSG INFKEFNFTV EGKQVYGVAS GCNLKNQNIL IFIHGSPGGW QNYYWYLGNK TLNKKFCIFA MDRPGFGNSN PNEVIPNVEK QAFILGKTIQ FFLNQFPLDK NTKIILVGHS YGAPIAARIS IVFSYKIRTL VLLAAPLSSK EEEIRWYNQI ADWTWVKNLL PLSLKNSNDE MFPLKSQLES LEKFWKLIPC KIILIHGKKD SLVSFHNLEF FKSIFSPSKL TTIELEKEDH FIPWTQKVFL EXXXLESIE
Validated NT Sequence
atcttactca cttattcaat tgactctaaa nnnnnntttt ctaaaaaaac tttttgagtc caaggaataa aatgatcttc tttttccagt tctatagtag tgagtttaga aggtgaaaat atagatttaa aaaattctaa attatgaaaa gatactaaag aatctttttt tccgtggatt aaaataattt tacagggaat caatttccaa aatttttcta aagattctaa ttgagattta agaggaaaca tttcgtcatt actatttttg agactgagtg gtaaaagatt tttaacccaa gtccaatctg caatttggtt ataccaacga atttcttctt ctttggaact tagaggtgca gcaagtaaaa cgagtgttct aatcttgtaa gaaaaaacta tagagattct tgcagcgatt ggggctccgt aagaatgtcc tacaagaatg attttagtat ttttgtctaa tgggaactga tttaaaaaaa attgaattgt ttttcctaag ataaaggctt gtttttccac gtttggaatc acttcgtttg ggtttgaatt tccgaaaccg ggtctgtcca tggcaaaaat acaaaatttt ttgtttaaag ttttatttcc caaataccaa taataatttt gccaacctcc gggagaacca tgtataaata ttaaaatatt ttgatttttt aaattacacc ctgatgcaac cccatagact tgttttcctt ccaccgtaaa attaaactct ttgaaattga ttccagaatt tttgagaagg attaaacttt cttttggctt ttttttaaaa tcctctggaa tgctcatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHSI PEDFKKKPKE SLILLKNSGI NFKEFNFTVE GKQVYGVASG CNLKNQNILI FIHGSPGGWQ NYYWYLGNKT LNKKFCIFAM DRPGFGNSNP NEVIPNVEKQ AFILGKTIQF FLNQFPLDKN TKIILVGHSY GAPIAARISI VFSYKIRTLV LLAAPLSSKE EEIRWYNQIA DWTWVKNLLP LSLKNSNDEM FPLKSQLESL EKFWKLIPCK IILIHGKKDS LVSFHNLEFF KSIFSPSKLT TIELEKEDHF IPWTQKVFLE KIFLESIE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catagcattc cagaggattt taaaaaaaag ccaaaagaaa gtttaatcct tctcaaaaat tctggaatca atttcaaaga gtttaatttt acggtggaag gaaaacaagt ctatggggtt gcatcagggt gtaatttaaa aaatcaaaat attttaatat ttatacatgg ttctcccgga ggttggcaaa attattattg gtatttggga aataaaactt taaacaaaaa attttgtatt tttgccatgg acagacccgg tttcggaaat tcaaacccaa acgaagtgat tccaaacgtg gaaaaacaag cctttatctt aggaaaaaca attcaatttt ttttaaatca gttcccatta gacaaaaata ctaaaatcat tcttgtagga cattcttacg gagccccaat cgctgcaaga atctctatag ttttttctta caagattaga acactcgttt tacttgctgc acctctaagt tccaaagaag aagaaattcg ttggtataac caaattgcag attggacttg ggttaaaaat cttttaccac tcagtctcaa aaatagtaat gacgaaatgt ttcctcttaa atctcaatta gaatctttag aaaaattttg gaaattgatt ccctgtaaaa ttattttaat ccacggaaaa aaagattctt tagtatcttt tcataattta gaatttttta aatctatatt ttcaccttct aaactcacta ctatagaact ggaaaaagaa gatcatttta ttccttggac tcaaaaagtt tttttagaaa agattttttt agagtcaatt gaatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat