LpinA.19796.a

Putative lipoprotein

CENTER ID: LpinA.19796.a
ORGANISM: Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LpinA.19796.a.B2.GE42340 mature protein 23 216
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
Molecular models
METHOD RESULTS
comparative modelling Robetta_2673
External Resources
RESOURCE REFERENCE ID
PATRIC ID: fig|267671.5.peg.3101
UniProt: Q72P88
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTFNTTIITV ITLFLFISGC TSSQKTVDSG LSQELVIRSK IQSIDSQLSS SQLDEKKRSS LLMEKAKLLI QIESYKEATV VLKEIQNSKE GKSLEHLDHY LGTAYLGIND TENAIVHFRK SESVDKNYES TNRKKMYAKA LYQEEKYGLA LGILGRASRE KDFEKDILFY ETVANSFMRI KEFKRCQMVL EEGLQKFPES PVLKEIQEQL SQVLPR
NT Sequence
atgacattca atacaacaat cattactgta ataaccttgt ttctatttat atctggttgt actagcagtc agaaaacagt ggattccgga ctttctcagg aattagtgat ccgttctaaa attcaatcga ttgattctca actttcctct tcgcaattag atgaaaaaaa acgttcttct ttgttgatgg aaaaggcaaa gttattgatt caaattgaat cttataaaga agcaacggtt gtcttaaaag agattcaaaa ttctaaagaa ggcaaaagtc tagaacatct agatcattat ttgggaacgg cttatttagg aatcaatgat actgaaaacg cgatcgttca ttttagaaag tccgagtccg tagataaaaa ctatgaatct acaaatcgta aaaagatgta tgcgaaagca ctctatcaag aagaaaaata cggcttggct ctgggcattt taggtcgtgc ttctagagaa aaagattttg aaaaagatat tctattttat gaaaccgtcg caaatagttt tatgcgaatt aaggaattca aaagatgtca gatggttctg gaagaagggc ttcagaaatt tccggaaagt ccggttctga aagaaatcca agagcaactt tctcaggttc ttccaagata a
Details for LpinA.19796.a.B2.GE42340
HARVESTED ON: 4/13/2018
SEQUENCED ON: 4/18/2018
EXPECTED MW: 23kDa
OBSERVED MW: 23kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS QKTVDSGLSQ ELVIRSKIQS IDSQLSSSQL DEKKRSSLLM EKAKLLIQIE SYKEATVVLK EIQNSKEGKS LEHLDHYLGT AYLGINDTEN AIVHFRKSES VDKNYESTNR KKMYAKALYQ EEKYGLALGI LGRASREKDF EKDILFYETV ANSFMRIKEF KRCQMVLEEG LQKFPESPVL KEIQEQLSQV LPR
Validated NT Sequence
atcttactca cttatcttgg aagaacctga gaaagttgct cttggatttc tttcagaacc ggactttccg gaaatttctg aagcccttct tccagaacca tctgacatct tttgaattcc ttaattcgca taaaactatt tgcgacggtt tcataaaata gaatatcttt ttcaaaatct ttttctctag aagcacgacc taaaatgccc agagccaagc cgtatttttc ttcttgatag agtgctttcg catacatctt tttacgattt gtagattcat agtttttatc tacggactcg gactttctaa aatgaacgat cgcgttttca gtatcattga ttcctaaata agccgttccc aaataatgat ctagatgttc tagacttttg ccttctttag aattttgaat ctcttttaag acaaccgttg cttctttata agattcaatt tgaatcaata actttgcctt ttccatcaac aaagaagaac gttttttttc atctaattgc gaagaggaaa gttgagaatc aatcgattga attttagaac ggatcactaa ttcctgagaa agtccggaat ccactgtttt ctgactcata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHSQ KTVDSGLSQE LVIRSKIQSI DSQLSSSQLD EKKRSSLLME KAKLLIQIES YKEATVVLKE IQNSKEGKSL EHLDHYLGTA YLGINDTENA IVHFRKSESV DKNYESTNRK KMYAKALYQE EKYGLALGIL GRASREKDFE KDILFYETVA NSFMRIKEFK RCQMVLEEGL QKFPESPVLK EIQEQLSQVL PR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catagtcaga aaacagtgga ttccggactt tctcaggaat tagtgatccg ttctaaaatt caatcgattg attctcaact ttcctcttcg caattagatg aaaaaaaacg ttcttctttg ttgatggaaa aggcaaagtt attgattcaa attgaatctt ataaagaagc aacggttgtc ttaaaagaga ttcaaaattc taaagaaggc aaaagtctag aacatctaga tcattatttg ggaacggctt atttaggaat caatgatact gaaaacgcga tcgttcattt tagaaagtcc gagtccgtag ataaaaacta tgaatctaca aatcgtaaaa agatgtatgc gaaagcactc tatcaagaag aaaaatacgg cttggctctg ggcattttag gtcgtgcttc tagagaaaaa gattttgaaa aagatattct attttatgaa accgtcgcaa atagttttat gcgaattaag gaattcaaaa gatgtcagat ggttctggaa gaagggcttc agaaatttcc ggaaagtccg gttctgaaag aaatccaaga gcaactttct caggttcttc caagatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat